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The map label for this gene is trmA [H]

Identifier: 92113465

GI number: 92113465

Start: 1531293

End: 1532468

Strand: Direct

Name: trmA [H]

Synonym: Csal_1339

Alternate gene names: 92113465

Gene position: 1531293-1532468 (Clockwise)

Preceding gene: 92113464

Following gene: 92113466

Centisome position: 41.42

GC content: 64.54

Gene sequence:

>1176_bases
ATGAGGCGGTATCGCCGTGGGCGAGACCGCGAGTTTTCGTTGAAAGGAGTTTTCGTGGCCGTACCCGTCGTCGAACCCGA
ACGTTACGAGGCCCAGCTCGACGCCAAGCGCGAGCGTGTCACGGCGCAATTCGCGCGCTTTTCACCGCCGGCGCTGGAGG
TCTTTCCCTCGCCGCCGAGCCATTACCGTCAGCGCTGCGAGTTTCGGCTGTGGCATGAAGGCGATGACCTGTTCTATGCC
ATGTTCGAGGTCGACCCGAGCGACCCCAAGCGCAAGGAGGTGATCCGCCTCGACGACTATCCTGTGGCCAGCGAGCGTAT
CAACGCCCTCATGCCGGCGTTGCGCGAGGCCCTGTTGGCCAGCGACGTGCTGCGGCGCAAGCTGTTCCAGGTTGAGTTCT
TGACCACCTTGAGCGGCGAGGCGCTCGTGACCTTGATCTATCACCGCCAGCTCGACGCGGCGTGGGAACAAGAGGCACGC
GCGCTGCAGACGACGCTGGGCGTATCGATCATCGGCCGGGCACGCAAGCAGCGCCTGGTGCTCGATCGTGACCATGTCTG
GGAGCGCCTGCAGGTCGAGGGGCGCGAGTTCGTCTATCAGCAGGTGGAAAACAGTTTTACCCAGCCCAATGCCGCGATTT
GCCAGTCGATGCTAGGCTGGGCGCGGGACGTGACCCGCGAGAGCCGCGAGGGCGACCTGGTGGAATTCTACTGCGGCAAC
GGCAATTTCACGGTGGCCCTGGCCGAGAACTTCCGGCGCGTGGTGGCCACCGAAATCTCCCGGACCTCGGTGGCGTCGGC
CAACGTCAATCTTGAAGCCAACGCCGTGGCCAATGCCGTGGTGGCGCGCATGTCCGCCGAGGAATTCAGCGCCGCCCTGG
CCGGCGAGAAGACCGGGCGGCGGGTGGCGGCGCTGGCGCTCGACGAGCACACCTTCACCACGGCCTTGGTGGACCCGCCG
CGGGCCGGACTCGATGCGCACAGCTGCGAGCAGCTCAAGGTCTATGAGCGCATCGTCTATATTTCCTGTAACCCGGATAC
GCTCGAGGCGAATCTCGAGCAATTGAGTGCCACGCATGTCGTGACCCGTTTCGCATTGTTCGATCAGTTTCCGTACACCG
ATCATTGCGAATGCGGCGTGTTGCTGGAGCGCCGGGCGCCGGCAGCGCGCGCGTGA

Upstream 100 bases:

>100_bases
CCGGGGCTGGCGCGGGCCCTGGCGGAATACGGTTTCAGCGCGCCGCGCGCCCTGTCGCTGCCCGACGTTTTCTCGGACAG
AGCTTGATCTGCTGATATTC

Downstream 100 bases:

>100_bases
GACCCCATGCACAACCGGCATACGCCATGAGGGAGCGTCACATCGGTGGGGTCGAGGGCGCGACAGGAGTAAGCTCGTAT
CACCGTGGCGATGCGTGCGG

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MRRYRRGRDREFSLKGVFVAVPVVEPERYEAQLDAKRERVTAQFARFSPPALEVFPSPPSHYRQRCEFRLWHEGDDLFYA
MFEVDPSDPKRKEVIRLDDYPVASERINALMPALREALLASDVLRRKLFQVEFLTTLSGEALVTLIYHRQLDAAWEQEAR
ALQTTLGVSIIGRARKQRLVLDRDHVWERLQVEGREFVYQQVENSFTQPNAAICQSMLGWARDVTRESREGDLVEFYCGN
GNFTVALAENFRRVVATEISRTSVASANVNLEANAVANAVVARMSAEEFSAALAGEKTGRRVAALALDEHTFTTALVDPP
RAGLDAHSCEQLKVYERIVYISCNPDTLEANLEQLSATHVVTRFALFDQFPYTDHCECGVLLERRAPAARA

Sequences:

>Translated_391_residues
MRRYRRGRDREFSLKGVFVAVPVVEPERYEAQLDAKRERVTAQFARFSPPALEVFPSPPSHYRQRCEFRLWHEGDDLFYA
MFEVDPSDPKRKEVIRLDDYPVASERINALMPALREALLASDVLRRKLFQVEFLTTLSGEALVTLIYHRQLDAAWEQEAR
ALQTTLGVSIIGRARKQRLVLDRDHVWERLQVEGREFVYQQVENSFTQPNAAICQSMLGWARDVTRESREGDLVEFYCGN
GNFTVALAENFRRVVATEISRTSVASANVNLEANAVANAVVARMSAEEFSAALAGEKTGRRVAALALDEHTFTTALVDPP
RAGLDAHSCEQLKVYERIVYISCNPDTLEANLEQLSATHVVTRFALFDQFPYTDHCECGVLLERRAPAARA
>Mature_391_residues
MRRYRRGRDREFSLKGVFVAVPVVEPERYEAQLDAKRERVTAQFARFSPPALEVFPSPPSHYRQRCEFRLWHEGDDLFYA
MFEVDPSDPKRKEVIRLDDYPVASERINALMPALREALLASDVLRRKLFQVEFLTTLSGEALVTLIYHRQLDAAWEQEAR
ALQTTLGVSIIGRARKQRLVLDRDHVWERLQVEGREFVYQQVENSFTQPNAAICQSMLGWARDVTRESREGDLVEFYCGN
GNFTVALAENFRRVVATEISRTSVASANVNLEANAVANAVVARMSAEEFSAALAGEKTGRRVAALALDEHTFTTALVDPP
RAGLDAHSCEQLKVYERIVYISCNPDTLEANLEQLSATHVVTRFALFDQFPYTDHCECGVLLERRAPAARA

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=193, Percent_Identity=27.4611398963731, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI51173878, Length=193, Percent_Identity=27.4611398963731, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790403, Length=361, Percent_Identity=53.185595567867, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1789148, Length=370, Percent_Identity=27.027027027027, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787083, Length=192, Percent_Identity=28.125, Blast_Score=69, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24666579, Length=199, Percent_Identity=28.643216080402, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011869
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.35 [H]

Molecular weight: Translated: 44400; Mature: 44400

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS01230 TRMA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRYRRGRDREFSLKGVFVAVPVVEPERYEAQLDAKRERVTAQFARFSPPALEVFPSPPS
CCCCCCCCCCCEEECEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHH
HYRQRCEFRLWHEGDDLFYAMFEVDPSDPKRKEVIRLDDYPVASERINALMPALREALLA
HHHHCCCEEEEECCCCEEEEEEEECCCCCCHHCEEEECCCCCHHHHHHHHHHHHHHHHHH
SDVLRRKLFQVEFLTTLSGEALVTLIYHRQLDAAWEQEARALQTTLGVSIIGRARKQRLV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LDRDHVWERLQVEGREFVYQQVENSFTQPNAAICQSMLGWARDVTRESREGDLVEFYCGN
CCHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
GNFTVALAENFRRVVATEISRTSVASANVNLEANAVANAVVARMSAEEFSAALAGEKTGR
CCEEEEEHHHHHHHHHHHHHHHEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RVAALALDEHTFTTALVDPPRAGLDAHSCEQLKVYERIVYISCNPDTLEANLEQLSATHV
EEEEEEECCCCEEEEEECCCCCCCCCCCHHHHEEEEEEEEEECCCCHHHHHHHHHHHHHH
VTRFALFDQFPYTDHCECGVLLERRAPAARA
HHHHHHHHCCCCCCCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MRRYRRGRDREFSLKGVFVAVPVVEPERYEAQLDAKRERVTAQFARFSPPALEVFPSPPS
CCCCCCCCCCCEEECEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHH
HYRQRCEFRLWHEGDDLFYAMFEVDPSDPKRKEVIRLDDYPVASERINALMPALREALLA
HHHHCCCEEEEECCCCEEEEEEEECCCCCCHHCEEEECCCCCHHHHHHHHHHHHHHHHHH
SDVLRRKLFQVEFLTTLSGEALVTLIYHRQLDAAWEQEARALQTTLGVSIIGRARKQRLV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LDRDHVWERLQVEGREFVYQQVENSFTQPNAAICQSMLGWARDVTRESREGDLVEFYCGN
CCHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
GNFTVALAENFRRVVATEISRTSVASANVNLEANAVANAVVARMSAEEFSAALAGEKTGR
CCEEEEEHHHHHHHHHHHHHHHEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RVAALALDEHTFTTALVDPPRAGLDAHSCEQLKVYERIVYISCNPDTLEANLEQLSATHV
EEEEEEECCCCEEEEEECCCCCCCCCCCHHHHEEEEEEEEEECCCCHHHHHHHHHHHHHH
VTRFALFDQFPYTDHCECGVLLERRAPAARA
HHHHHHHHCCCCCCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA