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The map label for this gene is miaA

Identifier: 92113402

GI number: 92113402

Start: 1456390

End: 1457328

Strand: Direct

Name: miaA

Synonym: Csal_1276

Alternate gene names: 92113402

Gene position: 1456390-1457328 (Clockwise)

Preceding gene: 92113401

Following gene: 92113403

Centisome position: 39.4

GC content: 66.24

Gene sequence:

>939_bases
ATGAGTGATACACGGCCACTGGCCCTTTTGCTGATGGGCCCGACGGCGGCCGGCAAGACCGATCTGGCCATCGCGCTACG
CGAGCGGCTGGGAGGAGAATTGATCAGCGTCGACTCGGCGATGATCTATCGTGGCATGGATATCGGCACCGCGAAGCCGT
CCGCCCAGGAGCTGGCGCGTGCGCCGCATCGCCTGATCGACATTCGCGATCCGGCCGAGACGTATTCCGCCGCCGAGTTT
CGCGACGATGCCCTGGCCGAGATGCGCGACATCTCGTCGCAGGGGCGTACGCCGATCCTCGTCGGTGGCACCATGATGTA
CATCAAGCGTTTGATCGATGGTGTGGCCTCGTTGCCGGCACGCGATCCGGCACTCCGGGAAGCGCTGAATGCCCGGGCCG
AGAGCGAGGGATTGGTGGCTCTGCACCGGGAGCTGTCGCGTGTCGATCCGGTAGCCGCCGAGACGATCCATCCGCACAAC
CGACAGCGATTGCTGCGGGCCCTGGAGGTCTATCAGCTCACGGGGCGGGCGCTGGGGGAACTGTGGGCCGAACAGGCCCG
CGAAACCTTTCCCTGGCGGCTCGTGTCCATAGCCCTGGCGCCGAATGCGCGCCACGTGCTGCATGCGCGCATCGCCGAGC
GCTTCGACAGCATGCTGGCGGCGGGCTTCCGCGATGAGGTGGCGGCGCTTCAGGCACGCGGAGACCTGCATCGAGGACTC
CCCGCCATACGTTGCGTGGGCTATCGTCAAATGTGGGAGCATCTGCGCGGCGAGACGGACGCGGCGACGATGCGCGAGCG
CGGCCTGGCCGCGACGCGACAACTCGCCAAGCGTCAGTTGACCTGGCTGCGCGGCTGGGAGGGCGTGCACTGGATCGACA
GCGATGCCTCGGACGCACATGAACAGGTATTGAAAATCGTGCGCGGAAGCAGCACTTAG

Upstream 100 bases:

>100_bases
CGGTCGTCCCACCTGGACCGAAATGTCGATGCATGAGCTGGACAAGTTGTTTCTACGCGGTCAGTGACCCCGCTGACCGG
CTACCGTTTCAGGACTTTCG

Downstream 100 bases:

>100_bases
CGTAAGATGTCCGGTCACGACAACGTCTGGCGAGCGTCTCCGCAAACGGCGGCGGCGGCCCGATCGGCGGCTGTGACCCT
TCGAAAACGACCATAAACCA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSDTRPLALLLMGPTAAGKTDLAIALRERLGGELISVDSAMIYRGMDIGTAKPSAQELARAPHRLIDIRDPAETYSAAEF
RDDALAEMRDISSQGRTPILVGGTMMYIKRLIDGVASLPARDPALREALNARAESEGLVALHRELSRVDPVAAETIHPHN
RQRLLRALEVYQLTGRALGELWAEQARETFPWRLVSIALAPNARHVLHARIAERFDSMLAAGFRDEVAALQARGDLHRGL
PAIRCVGYRQMWEHLRGETDAATMRERGLAATRQLAKRQLTWLRGWEGVHWIDSDASDAHEQVLKIVRGSST

Sequences:

>Translated_312_residues
MSDTRPLALLLMGPTAAGKTDLAIALRERLGGELISVDSAMIYRGMDIGTAKPSAQELARAPHRLIDIRDPAETYSAAEF
RDDALAEMRDISSQGRTPILVGGTMMYIKRLIDGVASLPARDPALREALNARAESEGLVALHRELSRVDPVAAETIHPHN
RQRLLRALEVYQLTGRALGELWAEQARETFPWRLVSIALAPNARHVLHARIAERFDSMLAAGFRDEVAALQARGDLHRGL
PAIRCVGYRQMWEHLRGETDAATMRERGLAATRQLAKRQLTWLRGWEGVHWIDSDASDAHEQVLKIVRGSST
>Mature_311_residues
SDTRPLALLLMGPTAAGKTDLAIALRERLGGELISVDSAMIYRGMDIGTAKPSAQELARAPHRLIDIRDPAETYSAAEFR
DDALAEMRDISSQGRTPILVGGTMMYIKRLIDGVASLPARDPALREALNARAESEGLVALHRELSRVDPVAAETIHPHNR
QRLLRALEVYQLTGRALGELWAEQARETFPWRLVSIALAPNARHVLHARIAERFDSMLAAGFRDEVAALQARGDLHRGLP
AIRCVGYRQMWEHLRGETDAATMRERGLAATRQLAKRQLTWLRGWEGVHWIDSDASDAHEQVLKIVRGSST

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=314, Percent_Identity=32.484076433121, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1790613, Length=312, Percent_Identity=58.0128205128205, Blast_Score=361, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25144712, Length=277, Percent_Identity=28.5198555956679, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324848, Length=289, Percent_Identity=28.719723183391, Blast_Score=130, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24649830, Length=236, Percent_Identity=37.2881355932203, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_CHRSD (Q1QY27)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_573330.1
- ProteinModelPortal:   Q1QY27
- SMR:   Q1QY27
- STRING:   Q1QY27
- GeneID:   4027749
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_1276
- NMPDR:   fig|290398.4.peg.1266
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- BioCyc:   CSAL290398:CSAL_1276-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34593; Mature: 34462

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTRPLALLLMGPTAAGKTDLAIALRERLGGELISVDSAMIYRGMDIGTAKPSAQELAR
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEECCHHHHHCCCCCCCCCCCHHHHHH
APHRLIDIRDPAETYSAAEFRDDALAEMRDISSQGRTPILVGGTMMYIKRLIDGVASLPA
CHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCC
RDPALREALNARAESEGLVALHRELSRVDPVAAETIHPHNRQRLLRALEVYQLTGRALGE
CCHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LWAEQARETFPWRLVSIALAPNARHVLHARIAERFDSMLAAGFRDEVAALQARGDLHRGL
HHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCC
PAIRCVGYRQMWEHLRGETDAATMRERGLAATRQLAKRQLTWLRGWEGVHWIDSDASDAH
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHH
EQVLKIVRGSST
HHHHHHHHCCCC
>Mature Secondary Structure 
SDTRPLALLLMGPTAAGKTDLAIALRERLGGELISVDSAMIYRGMDIGTAKPSAQELAR
CCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEECCHHHHHCCCCCCCCCCCHHHHHH
APHRLIDIRDPAETYSAAEFRDDALAEMRDISSQGRTPILVGGTMMYIKRLIDGVASLPA
CHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCC
RDPALREALNARAESEGLVALHRELSRVDPVAAETIHPHNRQRLLRALEVYQLTGRALGE
CCHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LWAEQARETFPWRLVSIALAPNARHVLHARIAERFDSMLAAGFRDEVAALQARGDLHRGL
HHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCC
PAIRCVGYRQMWEHLRGETDAATMRERGLAATRQLAKRQLTWLRGWEGVHWIDSDASDAH
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHH
EQVLKIVRGSST
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA