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The map label for this gene is truA [H]

Identifier: 92113385

GI number: 92113385

Start: 1437926

End: 1438798

Strand: Direct

Name: truA [H]

Synonym: Csal_1259

Alternate gene names: 92113385

Gene position: 1437926-1438798 (Clockwise)

Preceding gene: 92113384

Following gene: 92113386

Centisome position: 38.9

GC content: 65.98

Gene sequence:

>873_bases
ATGGGCGTCGAGTATGATGGCAGTGCGTATTGTGGCTGGCAGCGGCTCAAGCACGCTGCATCGGTGCAGGGGACGCTGGA
AGCCGCGCTGTCGCGGATCGCCAATGCGCCGGTTCAGGTCCTGTGCAGCGGGCGGACCGACAGTGGCGTGCATGCGACGC
GCCAGGTGGTGCATTTCGACGCCCCGGTGACACGCACCCAGAAGGCTTGGCTCTTCGGTGCCAATGCCAACCTGCCGCGC
GATGTCGCGGTACGCTGGGTCGTGCCGGTCGCCGACGATTTCCATGCACGCCTCTCCGCACTGGCCCGGCGCTATCGCTA
CGTGATCCTCAATCAGCCATCGCGGCCGGTGCTGGAGCGCGCCAATGCGACCTGGTGCCGTGAGCCTCTGGATGCCGAGC
GCATGCATGCCGCTGCCCAGGCGCTGGTCGGCGAGCATGACTTTTCCAGTTTTCGTGCCGCGGGTTGTCAGTCGAAGAGC
CGGTGGCGGCATGTGCACTTCATCGAGGTGCGTCGCTATGGCCAACTGGTGGTGATCGACATTCAGGCCAATGCCTTCTT
GCATCACATGATTCGCAATATCGCCGGCGCCCTGGTGGCGGTCGGGCGGGGCGATCACTCGACCGGTTACCTCGGCGAGC
TGCTGGCGCTTCGCGACCGGACGCAAGGCAATGTCACGGCGCCGGGATGTGGACTGCATTTCGTCGATGCGCTGTATGAC
GAGCGCTTCGTGTTGCCGAAGGAGCCGCTGGGGCCCAATCTGCTGGCGTTTCTCGGCGAGTGGACCGGGGAGCGCCGCCT
GCCCGATAGCGAGTGGGCGCGAGCGCGGCGTCAGCGTCGTGTCCATCCGCCGCGCCGGGAGGTGTCATCATGA

Upstream 100 bases:

>100_bases
GGCCGTTCCGTGATCGATATTCGAGGGTTTCGCGTGGCTGATTTCGATGGCATTTTTTGAGTTTCAGGATGATCGCCAGC
CGCTACAGGGGCGCCTTGCC

Downstream 100 bases:

>100_bases
CGTCGCCGATGCAGCGTACGCGCGTCAAGATCTGCGGCCTGACCCGGGAGGAGGACATCGACGCCGCGGTGGCGGCGGGC
GCCGATGCGCTGGGGTTCGT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MGVEYDGSAYCGWQRLKHAASVQGTLEAALSRIANAPVQVLCSGRTDSGVHATRQVVHFDAPVTRTQKAWLFGANANLPR
DVAVRWVVPVADDFHARLSALARRYRYVILNQPSRPVLERANATWCREPLDAERMHAAAQALVGEHDFSSFRAAGCQSKS
RWRHVHFIEVRRYGQLVVIDIQANAFLHHMIRNIAGALVAVGRGDHSTGYLGELLALRDRTQGNVTAPGCGLHFVDALYD
ERFVLPKEPLGPNLLAFLGEWTGERRLPDSEWARARRQRRVHPPRREVSS

Sequences:

>Translated_290_residues
MGVEYDGSAYCGWQRLKHAASVQGTLEAALSRIANAPVQVLCSGRTDSGVHATRQVVHFDAPVTRTQKAWLFGANANLPR
DVAVRWVVPVADDFHARLSALARRYRYVILNQPSRPVLERANATWCREPLDAERMHAAAQALVGEHDFSSFRAAGCQSKS
RWRHVHFIEVRRYGQLVVIDIQANAFLHHMIRNIAGALVAVGRGDHSTGYLGELLALRDRTQGNVTAPGCGLHFVDALYD
ERFVLPKEPLGPNLLAFLGEWTGERRLPDSEWARARRQRRVHPPRREVSS
>Mature_289_residues
GVEYDGSAYCGWQRLKHAASVQGTLEAALSRIANAPVQVLCSGRTDSGVHATRQVVHFDAPVTRTQKAWLFGANANLPRD
VAVRWVVPVADDFHARLSALARRYRYVILNQPSRPVLERANATWCREPLDAERMHAAAQALVGEHDFSSFRAAGCQSKSR
WRHVHFIEVRRYGQLVVIDIQANAFLHHMIRNIAGALVAVGRGDHSTGYLGELLALRDRTQGNVTAPGCGLHFVDALYDE
RFVLPKEPLGPNLLAFLGEWTGERRLPDSEWARARRQRRVHPPRREVSS

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=271, Percent_Identity=32.4723247232472, Blast_Score=85, Evalue=9e-17,
Organism=Escherichia coli, GI1788657, Length=256, Percent_Identity=55.859375, Blast_Score=294, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI71985046, Length=206, Percent_Identity=27.6699029126214, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 32463; Mature: 32332

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVEYDGSAYCGWQRLKHAASVQGTLEAALSRIANAPVQVLCSGRTDSGVHATRQVVHFD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHEEEEC
APVTRTQKAWLFGANANLPRDVAVRWVVPVADDFHARLSALARRYRYVILNQPSRPVLER
CCCCCCCEEEEEECCCCCCHHEEEEEEEECCCHHHHHHHHHHHHHEEEEECCCCCHHHHH
ANATWCREPLDAERMHAAAQALVGEHDFSSFRAAGCQSKSRWRHVHFIEVRRYGQLVVID
CCCHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHCCEEEEEEEECCCCEEEEE
IQANAFLHHMIRNIAGALVAVGRGDHSTGYLGELLALRDRTQGNVTAPGCGLHFVDALYD
ECHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHCCCCCCEECCCCCHHHHHHHHC
ERFVLPKEPLGPNLLAFLGEWTGERRLPDSEWARARRQRRVHPPRREVSS
CCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure 
GVEYDGSAYCGWQRLKHAASVQGTLEAALSRIANAPVQVLCSGRTDSGVHATRQVVHFD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHEEEEC
APVTRTQKAWLFGANANLPRDVAVRWVVPVADDFHARLSALARRYRYVILNQPSRPVLER
CCCCCCCEEEEEECCCCCCHHEEEEEEEECCCHHHHHHHHHHHHHEEEEECCCCCHHHHH
ANATWCREPLDAERMHAAAQALVGEHDFSSFRAAGCQSKSRWRHVHFIEVRRYGQLVVID
CCCHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHCCEEEEEEEECCCCEEEEE
IQANAFLHHMIRNIAGALVAVGRGDHSTGYLGELLALRDRTQGNVTAPGCGLHFVDALYD
ECHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHCCCCCCEECCCCCHHHHHHHHC
ERFVLPKEPLGPNLLAFLGEWTGERRLPDSEWARARRQRRVHPPRREVSS
CCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA