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The map label for this gene is purD [H]

Identifier: 92113037

GI number: 92113037

Start: 1022943

End: 1024241

Strand: Direct

Name: purD [H]

Synonym: Csal_0909

Alternate gene names: 92113037

Gene position: 1022943-1024241 (Clockwise)

Preceding gene: 92113036

Following gene: 92113038

Centisome position: 27.67

GC content: 68.51

Gene sequence:

>1299_bases
ATGAAGGTTTTGATCATCGGCGGCGGTGGCCGCGAGCACGCTCTCGCCTGGAAGGCAGCCCAATCCCCGCGCGTCGACGC
CGTTTTCGTGGCGCCGGGCAACGCCGGCACCGCGCGTGAGTCGAAGCTCACCAACGTGGCGATCGACGCCACGGACCTTG
CCGGTCTGGAGGCGTTCGCGAGAGACAACGGCGTCGACCTGACCATTGTCGGCCCCGAGGCTCCGCTGGTCGAAGGCGTG
GTCGATCGTTTCCGGGCCGCAGGGCTGGCGATCTTCGGCCCGACACAGGCCGCCGCCCAGCTGGAGGGATCCAAGGCCTT
CGCCAAGGATTTCCTCGCCCGGCATGCGATTCCCACCGCGGCCTACGCCACCTTCAGCGACGTCGAGCCGGCGCTGGCCT
ACGTGCGCGAGCAGGGGGCGCCGATCGTCATCAAGGCCGACGGGCTCGCCGCCGGCAAGGGCGTGATCGTCGCCATGAGC
GAGGCGGAGGCCGAGACGGCGGTGCGCGACATCCTGGCCGGCAACGCCTTCGGTGACGCCGGCGCCCGGGTGGTGATCGA
GGAGTTTCTGGAAGGCGAGGAAGCCAGCTTCATCGTCATGGTGGACGGCGAACACGTGCTGCCCATGGCCACCAGTCAGG
ATCACAAGCGTGCCGGAGATGGCGACACCGGACCCAACACCGGCGGCATGGGCGCCTACTCGCCGGCGCCGGTGGTGACC
CGGGAGGTCGAGAAACGCATCATGGACGAGGTCATACTGCCCACCGTGCGCGGCATGGCCGAAGAGGGACACCCCTATAC
CGGCTTTCTGTACGCTGGCTTGATGATCTCCCCGCAAGGTGCGCCCAAGGTGATCGAGTACAATTGCCGTTTCGGCGATC
CGGAAACCCAGCCGATCCTGCTGCGCTTGAAGTCGGACCTGGTGGGGCTCTGCCTGGCCGCGACCCGCGGGCAGCTCGCC
TCCCAGCAGTGCGACTGGGATACCCGCGCGGCGGTGGGCGTGGTCATGGCGGCGGGGGGCTATCCCGGTGATTATCGCAA
GGGCGACGTCATCGAGGGGCTCGACGGTGCCGAGGCCGTGGGATGCAAGGTCTTCCATGCCGGGACCGCCCTGCGCGACG
ATGGTCGAGTCGTGACCAGCGGCGGTCGCGTGCTATGTGTGACGGCCTTGGGCGCGCGCGTCACGGTGGCGCGCGAGCAT
GCCTACGAGGGGGTCGCGGCGATTCATTGGCAGGATGCGCTGTATCGTCGCGACATCGCGCATCGCGCCGTGGCGCGAGA
GCGCGACGCCAACACCTGA

Upstream 100 bases:

>100_bases
GACGACGACACCGAGCGCTGAGGGCTGGTCTGTCGCGGCGCGCGCTTTCTGACATAATAGTCGGATCATTTTCGTCATAG
CTCCATAAGGTCGTATCCAG

Downstream 100 bases:

>100_bases
GGGCACGGGGCATCACGCCCGCCAATCTAGAGCGACGAGCAGGGTGACGCGACATGGAACCGGTACGATCGAGCATCGCC
GGCCGCGCGGCCGGCATGAC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MKVLIIGGGGREHALAWKAAQSPRVDAVFVAPGNAGTARESKLTNVAIDATDLAGLEAFARDNGVDLTIVGPEAPLVEGV
VDRFRAAGLAIFGPTQAAAQLEGSKAFAKDFLARHAIPTAAYATFSDVEPALAYVREQGAPIVIKADGLAAGKGVIVAMS
EAEAETAVRDILAGNAFGDAGARVVIEEFLEGEEASFIVMVDGEHVLPMATSQDHKRAGDGDTGPNTGGMGAYSPAPVVT
REVEKRIMDEVILPTVRGMAEEGHPYTGFLYAGLMISPQGAPKVIEYNCRFGDPETQPILLRLKSDLVGLCLAATRGQLA
SQQCDWDTRAAVGVVMAAGGYPGDYRKGDVIEGLDGAEAVGCKVFHAGTALRDDGRVVTSGGRVLCVTALGARVTVAREH
AYEGVAAIHWQDALYRRDIAHRAVARERDANT

Sequences:

>Translated_432_residues
MKVLIIGGGGREHALAWKAAQSPRVDAVFVAPGNAGTARESKLTNVAIDATDLAGLEAFARDNGVDLTIVGPEAPLVEGV
VDRFRAAGLAIFGPTQAAAQLEGSKAFAKDFLARHAIPTAAYATFSDVEPALAYVREQGAPIVIKADGLAAGKGVIVAMS
EAEAETAVRDILAGNAFGDAGARVVIEEFLEGEEASFIVMVDGEHVLPMATSQDHKRAGDGDTGPNTGGMGAYSPAPVVT
REVEKRIMDEVILPTVRGMAEEGHPYTGFLYAGLMISPQGAPKVIEYNCRFGDPETQPILLRLKSDLVGLCLAATRGQLA
SQQCDWDTRAAVGVVMAAGGYPGDYRKGDVIEGLDGAEAVGCKVFHAGTALRDDGRVVTSGGRVLCVTALGARVTVAREH
AYEGVAAIHWQDALYRRDIAHRAVARERDANT
>Mature_432_residues
MKVLIIGGGGREHALAWKAAQSPRVDAVFVAPGNAGTARESKLTNVAIDATDLAGLEAFARDNGVDLTIVGPEAPLVEGV
VDRFRAAGLAIFGPTQAAAQLEGSKAFAKDFLARHAIPTAAYATFSDVEPALAYVREQGAPIVIKADGLAAGKGVIVAMS
EAEAETAVRDILAGNAFGDAGARVVIEEFLEGEEASFIVMVDGEHVLPMATSQDHKRAGDGDTGPNTGGMGAYSPAPVVT
REVEKRIMDEVILPTVRGMAEEGHPYTGFLYAGLMISPQGAPKVIEYNCRFGDPETQPILLRLKSDLVGLCLAATRGQLA
SQQCDWDTRAAVGVVMAAGGYPGDYRKGDVIEGLDGAEAVGCKVFHAGTALRDDGRVVTSGGRVLCVTALGARVTVAREH
AYEGVAAIHWQDALYRRDIAHRAVARERDANT

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=426, Percent_Identity=53.0516431924883, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI209869995, Length=426, Percent_Identity=53.0516431924883, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI209869993, Length=426, Percent_Identity=53.0516431924883, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI28416899, Length=426, Percent_Identity=53.0516431924883, Blast_Score=432, Evalue=1e-121,
Organism=Escherichia coli, GI1790438, Length=429, Percent_Identity=73.1934731934732, Blast_Score=634, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=424, Percent_Identity=42.2169811320755, Blast_Score=312, Evalue=3e-85,
Organism=Saccharomyces cerevisiae, GI6321203, Length=445, Percent_Identity=43.8202247191011, Blast_Score=358, Evalue=1e-99,
Organism=Drosophila melanogaster, GI62471619, Length=429, Percent_Identity=44.2890442890443, Blast_Score=328, Evalue=6e-90,
Organism=Drosophila melanogaster, GI24582400, Length=429, Percent_Identity=44.2890442890443, Blast_Score=327, Evalue=1e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45259; Mature: 45259

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLIIGGGGREHALAWKAAQSPRVDAVFVAPGNAGTARESKLTNVAIDATDLAGLEAFA
CEEEEEECCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHH
RDNGVDLTIVGPEAPLVEGVVDRFRAAGLAIFGPTQAAAQLEGSKAFAKDFLARHAIPTA
CCCCCEEEEECCCCHHHHHHHHHHHHCCEEEECCCHHHHHCCCCHHHHHHHHHHHCCCHH
AYATFSDVEPALAYVREQGAPIVIKADGLAAGKGVIVAMSEAEAETAVRDILAGNAFGDA
HHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCC
GARVVIEEFLEGEEASFIVMVDGEHVLPMATSQDHKRAGDGDTGPNTGGMGAYSPAPVVT
CHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
REVEKRIMDEVILPTVRGMAEEGHPYTGFLYAGLMISPQGAPKVIEYNCRFGDPETQPIL
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCEEEEECCCCCCCCCEEE
LRLKSDLVGLCLAATRGQLASQQCDWDTRAAVGVVMAAGGYPGDYRKGDVIEGLDGAEAV
EEEHHHHHHHHHHHHCCHHHHCCCCCCHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCHH
GCKVFHAGTALRDDGRVVTSGGRVLCVTALGARVTVAREHAYEGVAAIHWQDALYRRDIA
CEEEEECCCEECCCCEEEECCCCEEEEEECCCEEEEEHHHHHCCEEEEEHHHHHHHHHHH
HRAVARERDANT
HHHHHHHCCCCC
>Mature Secondary Structure
MKVLIIGGGGREHALAWKAAQSPRVDAVFVAPGNAGTARESKLTNVAIDATDLAGLEAFA
CEEEEEECCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHH
RDNGVDLTIVGPEAPLVEGVVDRFRAAGLAIFGPTQAAAQLEGSKAFAKDFLARHAIPTA
CCCCCEEEEECCCCHHHHHHHHHHHHCCEEEECCCHHHHHCCCCHHHHHHHHHHHCCCHH
AYATFSDVEPALAYVREQGAPIVIKADGLAAGKGVIVAMSEAEAETAVRDILAGNAFGDA
HHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCC
GARVVIEEFLEGEEASFIVMVDGEHVLPMATSQDHKRAGDGDTGPNTGGMGAYSPAPVVT
CHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
REVEKRIMDEVILPTVRGMAEEGHPYTGFLYAGLMISPQGAPKVIEYNCRFGDPETQPIL
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCEEEEECCCCCCCCCEEE
LRLKSDLVGLCLAATRGQLASQQCDWDTRAAVGVVMAAGGYPGDYRKGDVIEGLDGAEAV
EEEHHHHHHHHHHHHCCHHHHCCCCCCHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCHH
GCKVFHAGTALRDDGRVVTSGGRVLCVTALGARVTVAREHAYEGVAAIHWQDALYRRDIA
CEEEEECCCEECCCCEEEECCCCEEEEEECCCEEEEEHHHHHCCEEEEEHHHHHHHHHHH
HRAVARERDANT
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]