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The map label for this gene is rlmB [H]

Identifier: 92113015

GI number: 92113015

Start: 1000553

End: 1001338

Strand: Direct

Name: rlmB [H]

Synonym: Csal_0887

Alternate gene names: 92113015

Gene position: 1000553-1001338 (Clockwise)

Preceding gene: 92113014

Following gene: 92113016

Centisome position: 27.07

GC content: 68.96

Gene sequence:

>786_bases
ATGAAAGGTAAGTCGTCCCGGACGCGCGTCGCGACGCCCGAAGGTCTGGATGCCGTCTACGGCGTACATGCCGTGCGTGC
CTTGTTCGAGCGTGGCCAGCCGCCGCGCGAGCTGTGGATCCAGGAGGGGCGCGCCGAAGCGCGCCTGGAAGCGCTGGTGG
CGCTGGCGCGGCAGGCGGGCGTCAAGTGCGCCGGTGTGGCCCGCGAGACGCTCGATGGCGTGGCCAGAGGCGCCGTGCAT
CAGGGGGTGATCGCCTTTGCGCCGCCGCTGCAGGCCGAAAGCGAGGAGGCGTTATGGTTTCGCCTCGGCGCCTGGCAGCA
ATCGTCGCCGCCCTTGCTGCTGATCCTCGACGGGGTGACCGACCCGCATAATTTCGGTGCCTGTCTTCGCAGCGCGGATG
CGGCGGGCGCGCATGGCGTCATCGTGCCCAAGGACAAGGCCGCGCCGCTCAATGCCACGGTCCGCAAGGTCGCCTGCGGT
GCCGCCGATGTCACGCCCGTCTATCAAGTCACCAACCTGGCGCGGGCCATGGGTCGGCTCAAGACTCATGGGGTCTGGAT
CGTGGGTACCGCCGGCGAATCGGCGCAGTGCCTGTACGACATCGATCTGGCCGGGCCGCTGGCAGTGGTCATGGGCGCCG
AGGGCAAGGGCATGCGCCGCCTGACCCGCGATGCCTGTGACCTGCTCGCCAAGCTGCCCATGGCCGGCAGCGTTTCCAGT
CTCAACGTGTCGGTTGCCACCGGGGTCTGCCTGTTCGAGGCCGTTCGCCAGCGTCAGTCCTCGTGA

Upstream 100 bases:

>100_bases
TGCCACCAAGAAATCGCGCGACGGCAAGCCGCGCCGGCGTTCTCGGCGTGGCAAGGGAGACAAGCGCGGATGAGCGCCGG
ACACCGGAAAGTGAGGCCTC

Downstream 100 bases:

>100_bases
CGTCGGCCATGTAGCCCTTCGTGCGAGACGAGTTGCAAGCACGAGGGGCGATCACTACAATTGTGCGGTTCTCCGGGCGT
CGCTTGCGCGTGCCCTGGAG

Product: RNA methyltransferase TrmH

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKGKSSRTRVATPEGLDAVYGVHAVRALFERGQPPRELWIQEGRAEARLEALVALARQAGVKCAGVARETLDGVARGAVH
QGVIAFAPPLQAESEEALWFRLGAWQQSSPPLLLILDGVTDPHNFGACLRSADAAGAHGVIVPKDKAAPLNATVRKVACG
AADVTPVYQVTNLARAMGRLKTHGVWIVGTAGESAQCLYDIDLAGPLAVVMGAEGKGMRRLTRDACDLLAKLPMAGSVSS
LNVSVATGVCLFEAVRQRQSS

Sequences:

>Translated_261_residues
MKGKSSRTRVATPEGLDAVYGVHAVRALFERGQPPRELWIQEGRAEARLEALVALARQAGVKCAGVARETLDGVARGAVH
QGVIAFAPPLQAESEEALWFRLGAWQQSSPPLLLILDGVTDPHNFGACLRSADAAGAHGVIVPKDKAAPLNATVRKVACG
AADVTPVYQVTNLARAMGRLKTHGVWIVGTAGESAQCLYDIDLAGPLAVVMGAEGKGMRRLTRDACDLLAKLPMAGSVSS
LNVSVATGVCLFEAVRQRQSS
>Mature_261_residues
MKGKSSRTRVATPEGLDAVYGVHAVRALFERGQPPRELWIQEGRAEARLEALVALARQAGVKCAGVARETLDGVARGAVH
QGVIAFAPPLQAESEEALWFRLGAWQQSSPPLLLILDGVTDPHNFGACLRSADAAGAHGVIVPKDKAAPLNATVRKVACG
AADVTPVYQVTNLARAMGRLKTHGVWIVGTAGESAQCLYDIDLAGPLAVVMGAEGKGMRRLTRDACDLLAKLPMAGSVSS
LNVSVATGVCLFEAVRQRQSS

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=242, Percent_Identity=31.8181818181818, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1790623, Length=244, Percent_Identity=57.7868852459016, Blast_Score=268, Evalue=2e-73,
Organism=Escherichia coli, GI1788935, Length=243, Percent_Identity=29.6296296296296, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1790083, Length=157, Percent_Identity=34.3949044585987, Blast_Score=78, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17555908, Length=264, Percent_Identity=26.5151515151515, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324775, Length=165, Percent_Identity=29.6969696969697, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27423; Mature: 27423

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGKSSRTRVATPEGLDAVYGVHAVRALFERGQPPRELWIQEGRAEARLEALVALARQAG
CCCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHC
VKCAGVARETLDGVARGAVHQGVIAFAPPLQAESEEALWFRLGAWQQSSPPLLLILDGVT
CCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCC
DPHNFGACLRSADAAGAHGVIVPKDKAAPLNATVRKVACGAADVTPVYQVTNLARAMGRL
CCHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
KTHGVWIVGTAGESAQCLYDIDLAGPLAVVMGAEGKGMRRLTRDACDLLAKLPMAGSVSS
HHCCEEEEECCCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCC
LNVSVATGVCLFEAVRQRQSS
CEEHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKGKSSRTRVATPEGLDAVYGVHAVRALFERGQPPRELWIQEGRAEARLEALVALARQAG
CCCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHC
VKCAGVARETLDGVARGAVHQGVIAFAPPLQAESEEALWFRLGAWQQSSPPLLLILDGVT
CCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCC
DPHNFGACLRSADAAGAHGVIVPKDKAAPLNATVRKVACGAADVTPVYQVTNLARAMGRL
CCHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
KTHGVWIVGTAGESAQCLYDIDLAGPLAVVMGAEGKGMRRLTRDACDLLAKLPMAGSVSS
HHCCEEEEECCCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCC
LNVSVATGVCLFEAVRQRQSS
CEEHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]