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The map label for this gene is aceF [C]

Identifier: 92112984

GI number: 92112984

Start: 958102

End: 960189

Strand: Direct

Name: aceF [C]

Synonym: Csal_0856

Alternate gene names: 92112984

Gene position: 958102-960189 (Clockwise)

Preceding gene: 92112983

Following gene: 92112985

Centisome position: 25.92

GC content: 64.99

Gene sequence:

>2088_bases
TTGAGTAGCGAAATCATCAAAGTTCCCGATATCGGCGGCAGCGAAGGCGTCGAGATCATCGAGGTGACGGTGTCCGAGGG
CGATGTCATCGCGCCCGAGGACACCATGATCACGCTGGAATCCGACAAGGCCAGCATGGACGTCCCCGCGCCCAAGGGCG
GTAAGGTCGTCAAGGTGCTGGTCAAGGAAGGCGATACCGTCTCCGAGGGCGACGACATCCTCGAGCTCGAAGCGGAAGGC
GGTGGCGACGACGCCGGTGATACCGCCTCTCAGGACGAGGAAGCGTCACAGGACGCGAGTGCCTCCGAAGAGCAGGAAAT
GGCGGCACCCGAAAAGGCGGCGCCGAAGAAGTCCGGCGGTGGCGGCAAGCGTACCGTCGAGGTCAAGGTGCCGGATATCG
GCGGCAGTGAAGGCGTCGAGATCATCGAGGTACCGGTCAGCGAAGGCGACGAGATCGAGGCGGAAGACACCTTGATCACG
CTGGAGTCCGACAAGGCGTCCATGGACGTCCCGAGCCCGTACAGTGGCAAGCTGGTCTCGCTGGCGGTCAAGGAAGGCGA
CAGTGTTTCCGAAGGCGATGTGATCGGCAGCATGGAGATCGCCGGCGAAGGCGGGGACGACGACGAGACCGACGCGGCAC
CGGCGGAAGACGACGCGAGCAGCGCCCTGGCGGAAGACGAGGCCGACGGCGATGACAGCGAAGAGGACGCAGGCGAAGGC
GAGTCCGGCCGCAAGGAACTTCGCGTCCCCGATATCGGCGGCAGCGAAGGCGTCGAGATCATCGAGGTTGCCATCGGCGA
AGGTGACGAGATCGGCGAGGAAGATACGCTGATCACGCTGGAGTCCGACAAGGCCTCCATGGACGTTCCCAGCCCCTACA
AGGGCAAGGTGGTCGAAGTCTCGGTCAAGGAAGGCGACAGCGTTTCCGAGGGCGACCTGATCGGCTACATCGAGGTGGCC
GGCACTCCGGTGGCCGGCGCGAAGAAGCCGGCCAAGAAGTCCGATGGCGGCAAGAAGTCCGGCGCCGAGAAAGCCGACGC
CGGTCAGCAAAAGGCGACTGACTCCCGGCCGGCGCCGGCCAAGTCCGATGCCAAACGCGCTGCGCAGCCTGCAGGCGAGC
CGAGTCCCGAGGCACGTCACAAGCACGGCGACAAGAAGACTGCCGGTGCGCACGTGCATGCCGGGCCGGCGGTCCGCATG
CTGGCGCGTGAACTGGGCGTCGACCTGGCTCAGGTCACGCCCACCGGGCCCAAGAGTCGCGTACTGAAGGAAGACGTCCA
GGGTTACGTCAAGAACGCCCTTCAGCAGCGCGCCAGCGGCACCGGAAGCACGGCCGCGCCGACGGACGGTGCGGGCATCC
CGGCGGTGCCGGACATCGACTTCAGCCAGTTCGGCGAGGTCGAGGAAAAGCCCATGGGCCGCCTGATGAAGGCCGGTGCG
GCCAACCTGCATCGCAGCTGGCTCAACGTGCCGCACGTGACCCAGTTCGACGAAGCGGACATCACCGAGCTCGAGGCCTT
CCGCAAGTCGATGAAGGACGAGGCTGCCAAGCAGGACGCCAAGCTCACGCCGCTGCCGTTCCTGATCAAGGCCTGCGCCT
ATGCCTTGCAGAAATTCCCGCAGTTCAACGTCAGCCTGAAGAGCGACGGCGAGACCCTGGTGCAGAAGCATTACGTGCAC
ATCGGTATCGCCGTGGATACGCCGGACGGCCTGATGGTGCCGGTGATCCGCAACGCCGATCAGAAGTCGCTGATCGAGCT
GGCCAAGGAATCGGTCGAACTGGCCGGCAAGGCACAGTCGAAGAAGCTCAAGCGCGAGGAGATGACGGGCGGCTGCTTCA
CCATCTCCAGCCTGGGTTCCATCGGTGGGACTGCCTTCACGCCCATCGTCAACGCCCCGGAAGTCGCCATCCTCGGCGTC
TCCAAGGCGCAGATGAAGCCGGTGTGGGACGGTGCGGAGTTCGCCCCAAGGCTGATGATGCCGCTGTCGCTGTCCTACGA
TCACCGCGCGGTCAACGGTGCGGATGCGGCGCGCTTCACGGCCTTCCTGGCCCAGGCGCTGAGCGATATTCGTCGCCTGT
TGATGTAA

Upstream 100 bases:

>100_bases
GCGGTGGCGGCCATGGCCAGATTGCCTGGTCTGGTCGCGTCACCCGGTCGGCAGCGAGGACCGGAGCGACCAGACCGCCA
GATTGGAAAGGAGCGCGACC

Downstream 100 bases:

>100_bases
GCGGACGCATGCGCATCTTCGGCATGCCGAAAGCGGCTCCCCTCGGGGAGCCGCTTTTTTTCGTCGGGTGCTTGACGAGA
GGGGGCGTCCGGCGAGGAAT

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 695; Mature: 694

Protein sequence:

>695_residues
MSSEIIKVPDIGGSEGVEIIEVTVSEGDVIAPEDTMITLESDKASMDVPAPKGGKVVKVLVKEGDTVSEGDDILELEAEG
GGDDAGDTASQDEEASQDASASEEQEMAAPEKAAPKKSGGGGKRTVEVKVPDIGGSEGVEIIEVPVSEGDEIEAEDTLIT
LESDKASMDVPSPYSGKLVSLAVKEGDSVSEGDVIGSMEIAGEGGDDDETDAAPAEDDASSALAEDEADGDDSEEDAGEG
ESGRKELRVPDIGGSEGVEIIEVAIGEGDEIGEEDTLITLESDKASMDVPSPYKGKVVEVSVKEGDSVSEGDLIGYIEVA
GTPVAGAKKPAKKSDGGKKSGAEKADAGQQKATDSRPAPAKSDAKRAAQPAGEPSPEARHKHGDKKTAGAHVHAGPAVRM
LARELGVDLAQVTPTGPKSRVLKEDVQGYVKNALQQRASGTGSTAAPTDGAGIPAVPDIDFSQFGEVEEKPMGRLMKAGA
ANLHRSWLNVPHVTQFDEADITELEAFRKSMKDEAAKQDAKLTPLPFLIKACAYALQKFPQFNVSLKSDGETLVQKHYVH
IGIAVDTPDGLMVPVIRNADQKSLIELAKESVELAGKAQSKKLKREEMTGGCFTISSLGSIGGTAFTPIVNAPEVAILGV
SKAQMKPVWDGAEFAPRLMMPLSLSYDHRAVNGADAARFTAFLAQALSDIRRLLM

Sequences:

>Translated_695_residues
MSSEIIKVPDIGGSEGVEIIEVTVSEGDVIAPEDTMITLESDKASMDVPAPKGGKVVKVLVKEGDTVSEGDDILELEAEG
GGDDAGDTASQDEEASQDASASEEQEMAAPEKAAPKKSGGGGKRTVEVKVPDIGGSEGVEIIEVPVSEGDEIEAEDTLIT
LESDKASMDVPSPYSGKLVSLAVKEGDSVSEGDVIGSMEIAGEGGDDDETDAAPAEDDASSALAEDEADGDDSEEDAGEG
ESGRKELRVPDIGGSEGVEIIEVAIGEGDEIGEEDTLITLESDKASMDVPSPYKGKVVEVSVKEGDSVSEGDLIGYIEVA
GTPVAGAKKPAKKSDGGKKSGAEKADAGQQKATDSRPAPAKSDAKRAAQPAGEPSPEARHKHGDKKTAGAHVHAGPAVRM
LARELGVDLAQVTPTGPKSRVLKEDVQGYVKNALQQRASGTGSTAAPTDGAGIPAVPDIDFSQFGEVEEKPMGRLMKAGA
ANLHRSWLNVPHVTQFDEADITELEAFRKSMKDEAAKQDAKLTPLPFLIKACAYALQKFPQFNVSLKSDGETLVQKHYVH
IGIAVDTPDGLMVPVIRNADQKSLIELAKESVELAGKAQSKKLKREEMTGGCFTISSLGSIGGTAFTPIVNAPEVAILGV
SKAQMKPVWDGAEFAPRLMMPLSLSYDHRAVNGADAARFTAFLAQALSDIRRLLM
>Mature_694_residues
SSEIIKVPDIGGSEGVEIIEVTVSEGDVIAPEDTMITLESDKASMDVPAPKGGKVVKVLVKEGDTVSEGDDILELEAEGG
GDDAGDTASQDEEASQDASASEEQEMAAPEKAAPKKSGGGGKRTVEVKVPDIGGSEGVEIIEVPVSEGDEIEAEDTLITL
ESDKASMDVPSPYSGKLVSLAVKEGDSVSEGDVIGSMEIAGEGGDDDETDAAPAEDDASSALAEDEADGDDSEEDAGEGE
SGRKELRVPDIGGSEGVEIIEVAIGEGDEIGEEDTLITLESDKASMDVPSPYKGKVVEVSVKEGDSVSEGDLIGYIEVAG
TPVAGAKKPAKKSDGGKKSGAEKADAGQQKATDSRPAPAKSDAKRAAQPAGEPSPEARHKHGDKKTAGAHVHAGPAVRML
ARELGVDLAQVTPTGPKSRVLKEDVQGYVKNALQQRASGTGSTAAPTDGAGIPAVPDIDFSQFGEVEEKPMGRLMKAGAA
NLHRSWLNVPHVTQFDEADITELEAFRKSMKDEAAKQDAKLTPLPFLIKACAYALQKFPQFNVSLKSDGETLVQKHYVHI
GIAVDTPDGLMVPVIRNADQKSLIELAKESVELAGKAQSKKLKREEMTGGCFTISSLGSIGGTAFTPIVNAPEVAILGVS
KAQMKPVWDGAEFAPRLMMPLSLSYDHRAVNGADAARFTAFLAQALSDIRRLLM

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=467, Percent_Identity=28.6937901498929, Blast_Score=159, Evalue=7e-39,
Organism=Homo sapiens, GI31711992, Length=433, Percent_Identity=28.6374133949192, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI19923748, Length=225, Percent_Identity=35.1111111111111, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI203098816, Length=465, Percent_Identity=26.8817204301075, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI203098753, Length=465, Percent_Identity=26.8817204301075, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI260898739, Length=163, Percent_Identity=34.3558282208589, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1786305, Length=695, Percent_Identity=43.3093525179856, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI1786946, Length=429, Percent_Identity=27.2727272727273, Blast_Score=148, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17537937, Length=438, Percent_Identity=30.5936073059361, Blast_Score=180, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17560088, Length=438, Percent_Identity=32.1917808219178, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI25146366, Length=206, Percent_Identity=38.8349514563107, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17538894, Length=320, Percent_Identity=30, Blast_Score=118, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6320352, Length=244, Percent_Identity=35.655737704918, Blast_Score=154, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6324258, Length=440, Percent_Identity=26.5909090909091, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI18859875, Length=460, Percent_Identity=30.6521739130435, Blast_Score=163, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24645909, Length=209, Percent_Identity=37.3205741626794, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24582497, Length=232, Percent_Identity=30.6034482758621, Blast_Score=112, Evalue=9e-25,
Organism=Drosophila melanogaster, GI20129315, Length=226, Percent_Identity=31.4159292035398, Blast_Score=110, Evalue=5e-24,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 72488; Mature: 72357

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEIIKVPDIGGSEGVEIIEVTVSEGDVIAPEDTMITLESDKASMDVPAPKGGKVVKVL
CCCCEEECCCCCCCCCCEEEEEEECCCCEECCCCCEEEEECCCCCCCCCCCCCCCEEEEE
VKEGDTVSEGDDILELEAEGGGDDAGDTASQDEEASQDASASEEQEMAAPEKAAPKKSGG
EECCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCCCHHHHHCCCHHCCCCCCCC
GGKRTVEVKVPDIGGSEGVEIIEVPVSEGDEIEAEDTLITLESDKASMDVPSPYSGKLVS
CCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEE
LAVKEGDSVSEGDVIGSMEIAGEGGDDDETDAAPAEDDASSALAEDEADGDDSEEDAGEG
EEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHCCCC
ESGRKELRVPDIGGSEGVEIIEVAIGEGDEIGEEDTLITLESDKASMDVPSPYKGKVVEV
CCCCCEECCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEE
SVKEGDSVSEGDLIGYIEVAGTPVAGAKKPAKKSDGGKKSGAEKADAGQQKATDSRPAPA
EECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHCCCCCCCCC
KSDAKRAAQPAGEPSPEARHKHGDKKTAGAHVHAGPAVRMLARELGVDLAQVTPTGPKSR
CHHHHHHHCCCCCCCCHHHHHCCCCCCCCCEEECCHHHHHHHHHHCCCEEECCCCCCHHH
VLKEDVQGYVKNALQQRASGTGSTAAPTDGAGIPAVPDIDFSQFGEVEEKPMGRLMKAGA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHH
ANLHRSWLNVPHVTQFDEADITELEAFRKSMKDEAAKQDAKLTPLPFLIKACAYALQKFP
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
QFNVSLKSDGETLVQKHYVHIGIAVDTPDGLMVPVIRNADQKSLIELAKESVELAGKAQS
CCCEEECCCCHHHHHEEEEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHCCHHH
KKLKREEMTGGCFTISSLGSIGGTAFTPIVNAPEVAILGVSKAQMKPVWDGAEFAPRLMM
HHHHHHHCCCCEEEHHHHHCCCCCEECCCCCCCCEEEEECCHHHCCCCCCCHHHCCHHHC
PLSLSYDHRAVNGADAARFTAFLAQALSDIRRLLM
CEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SSEIIKVPDIGGSEGVEIIEVTVSEGDVIAPEDTMITLESDKASMDVPAPKGGKVVKVL
CCCEEECCCCCCCCCCEEEEEEECCCCEECCCCCEEEEECCCCCCCCCCCCCCCEEEEE
VKEGDTVSEGDDILELEAEGGGDDAGDTASQDEEASQDASASEEQEMAAPEKAAPKKSGG
EECCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCCCHHHHHCCCHHCCCCCCCC
GGKRTVEVKVPDIGGSEGVEIIEVPVSEGDEIEAEDTLITLESDKASMDVPSPYSGKLVS
CCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEE
LAVKEGDSVSEGDVIGSMEIAGEGGDDDETDAAPAEDDASSALAEDEADGDDSEEDAGEG
EEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHCCCC
ESGRKELRVPDIGGSEGVEIIEVAIGEGDEIGEEDTLITLESDKASMDVPSPYKGKVVEV
CCCCCEECCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEE
SVKEGDSVSEGDLIGYIEVAGTPVAGAKKPAKKSDGGKKSGAEKADAGQQKATDSRPAPA
EECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHCCCCCCCCC
KSDAKRAAQPAGEPSPEARHKHGDKKTAGAHVHAGPAVRMLARELGVDLAQVTPTGPKSR
CHHHHHHHCCCCCCCCHHHHHCCCCCCCCCEEECCHHHHHHHHHHCCCEEECCCCCCHHH
VLKEDVQGYVKNALQQRASGTGSTAAPTDGAGIPAVPDIDFSQFGEVEEKPMGRLMKAGA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHH
ANLHRSWLNVPHVTQFDEADITELEAFRKSMKDEAAKQDAKLTPLPFLIKACAYALQKFP
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
QFNVSLKSDGETLVQKHYVHIGIAVDTPDGLMVPVIRNADQKSLIELAKESVELAGKAQS
CCCEEECCCCHHHHHEEEEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHCCHHH
KKLKREEMTGGCFTISSLGSIGGTAFTPIVNAPEVAILGVSKAQMKPVWDGAEFAPRLMM
HHHHHHHCCCCEEEHHHHHCCCCCEECCCCCCCCEEEEECCHHHCCCCCCCHHHCCHHHC
PLSLSYDHRAVNGADAARFTAFLAQALSDIRRLLM
CEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3292237; 3691494; 3191993; 1549782; 8068086; 9119000 [H]