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The map label for this gene is ampD [H]

Identifier: 92112982

GI number: 92112982

Start: 954365

End: 954958

Strand: Direct

Name: ampD [H]

Synonym: Csal_0854

Alternate gene names: 92112982

Gene position: 954365-954958 (Clockwise)

Preceding gene: 92112980

Following gene: 92112983

Centisome position: 25.82

GC content: 66.16

Gene sequence:

>594_bases
ATGCAAGTCGATCCTCATCATTGGCTGACGGCGGCGCGGCACGTCGCCTCGCCCAATCATAACGCACGGCCCGAAGACGA
GGTGTCGGCGCTGGTGCTGCATTCCATCAGCTTGCCCCCGGGGCGCTTCGGGGGCGATGCCATCGAACGGTTGTTCACCA
ATCGTCTCGATCCCGCCGCGCATCCCTACTTTGCCACGATCCATGCCCTGCGGGTGTCGGCGCATGTGCTGATCCGCCGC
GATGGCGAAGCGGTACAGTTCGTGGGCTTCGACCGCCGCGCCTGGCATGCCGGGCGCTCGCGCTGGTGGGACGGACATCG
CGAACGTCGCGAACTCAACGATTTCAGCATCGGCATCGAGCTGGAAGGCGACGAGATTCACGCCTACCGCGAGGCACAGT
ATCGTTGCCTGGCGCGGGTGTTCCTGGCACTGCATGCGCGCTATCCCGCCTTGACGCTGGCGCGCGTGACCAGCCACGCG
CGAATTGCGCCGCTGCGCAAGACCGACCCGGGGCCGGCCTTCGACTGGGCCTACTTTCGCCAGTGCGTGCAGGCGTTGAC
GACGCAGGCAAGCGATGCCCGGGCATGCGTGTAA

Upstream 100 bases:

>100_bases
GGTGTGCGACAATACTCGCATTATATCGGGATGACGCCGAGGCGGTCAGGGGGTGTGCAGCCCCGTGTCACGGCGTATCG
TCAGGTTGGCAGGAGCACGC

Downstream 100 bases:

>100_bases
TTCCACTACATCGTGTAAGCCTTTGTCGCATGCCGAGGGCGATGCCGCTTTCCATGCCATGCATCAGCATTTTGCAACCC
GCTTGCGACCTATCGAATTG

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MQVDPHHWLTAARHVASPNHNARPEDEVSALVLHSISLPPGRFGGDAIERLFTNRLDPAAHPYFATIHALRVSAHVLIRR
DGEAVQFVGFDRRAWHAGRSRWWDGHRERRELNDFSIGIELEGDEIHAYREAQYRCLARVFLALHARYPALTLARVTSHA
RIAPLRKTDPGPAFDWAYFRQCVQALTTQASDARACV

Sequences:

>Translated_197_residues
MQVDPHHWLTAARHVASPNHNARPEDEVSALVLHSISLPPGRFGGDAIERLFTNRLDPAAHPYFATIHALRVSAHVLIRR
DGEAVQFVGFDRRAWHAGRSRWWDGHRERRELNDFSIGIELEGDEIHAYREAQYRCLARVFLALHARYPALTLARVTSHA
RIAPLRKTDPGPAFDWAYFRQCVQALTTQASDARACV
>Mature_197_residues
MQVDPHHWLTAARHVASPNHNARPEDEVSALVLHSISLPPGRFGGDAIERLFTNRLDPAAHPYFATIHALRVSAHVLIRR
DGEAVQFVGFDRRAWHAGRSRWWDGHRERRELNDFSIGIELEGDEIHAYREAQYRCLARVFLALHARYPALTLARVTSHA
RIAPLRKTDPGPAFDWAYFRQCVQALTTQASDARACV

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=182, Percent_Identity=58.2417582417582, Blast_Score=197, Evalue=4e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 22398; Mature: 22398

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVDPHHWLTAARHVASPNHNARPEDEVSALVLHSISLPPGRFGGDAIERLFTNRLDPAA
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCC
HPYFATIHALRVSAHVLIRRDGEAVQFVGFDRRAWHAGRSRWWDGHRERRELNDFSIGIE
CCHHHHHHHHHHHHEEEEEECCCEEEEECCCHHHHHHCHHHCCCCHHHHHHCCCEEEEEE
LEGDEIHAYREAQYRCLARVFLALHARYPALTLARVTSHARIAPLRKTDPGPAFDWAYFR
ECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHCCCCCCCCCCCCCCHHHHH
QCVQALTTQASDARACV
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQVDPHHWLTAARHVASPNHNARPEDEVSALVLHSISLPPGRFGGDAIERLFTNRLDPAA
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCC
HPYFATIHALRVSAHVLIRRDGEAVQFVGFDRRAWHAGRSRWWDGHRERRELNDFSIGIE
CCHHHHHHHHHHHHEEEEEECCCEEEEECCCHHHHHHCHHHCCCCHHHHHHCCCEEEEEE
LEGDEIHAYREAQYRCLARVFLALHARYPALTLARVTSHARIAPLRKTDPGPAFDWAYFR
ECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHCCCCCCCCCCCCCCHHHHH
QCVQALTTQASDARACV
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]