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The map label for this gene is yiaO [H]

Identifier: 92112792

GI number: 92112792

Start: 738316

End: 739353

Strand: Direct

Name: yiaO [H]

Synonym: Csal_0660

Alternate gene names: 92112792

Gene position: 738316-739353 (Clockwise)

Preceding gene: 92112791

Following gene: 92112793

Centisome position: 19.97

GC content: 60.12

Gene sequence:

>1038_bases
ATGCGTATCCTCACCCCGCGCAACGTCCTGCTGGCCGCCGTCACGACCGGCATGATGGCCACCGTGAGCCTGGCCAATGC
GGCGACCACCCTCAATCTGAGCTACAACGGCCCCCCCGATACCGACAAGAATGCCGTGCACCTCTTTGCCAGCAACCTCA
AGCGACTGGTAGAGGAGAAGACCGATGGCGACATTCAGCTCAAGCTTTACCCCAACTCCATGCTCGGCGAAGAGCAGGAA
CGCATGGAGCAGGTCATCAATACCCCGAGCTTGAACATTGCATCGTTCGCCGGCCTGTCGCCGATCGTGCCGGAGATTTA
TGTCAGCGCGATTCCCTTCTTGTTCGAGGACTATGAAGCCGCCCACCAGTTCTTCGACGAGGGCGATTACTGGAACAAGG
TCGAGGATACTCTCGAGGAGCGTACCGGCGCCGAGCTGCTCGGTGTCATCGAGGAGGGCGGCTTCCTCGACTTCACCAAC
TCGAAGCGTCCCATCAGCAGTCCGGAGGATTTCGAGGGCCTGCGCTTTCGTGCCATGGACCCCAGCCAGGTAGCGCTTTA
CGAGGCCTTTGGTGCCTCCGGCACGCCGATCCCCTGGACCGATACCTACATGGCGCTCAAGACCAACGTCGCCGATGGCC
AGATGAACCCGCCGATGTACATCATCATGGGCAGCCTCTACGAGGTGCAGAAGTATCTGACACTGGCCAATGTGCAGTAT
TCGGACCAGTTCTTGATTGCCAACGGCGAATGGTACGACGACCTTTCCGAAGAAAACCGCCAAGCGATCGAGGCGGCTGT
CCAGGAAGCCAGCGAGCTCAACCGGGAAGATGTCGAGAAGCGGGTCGACGAGCGCATCCAGTTCCTGGCCGACCAGGGCA
TGGAAGTCATCGAGCCCACCGAGGATGAGCTTGCTGCCTTCCGCGAGAAGGGGCAGCCGGCCTATATCGAGTGGCTGACG
GACGAGCAGGGCATTGACCGGGCATGGATCGAAATGGCCCTCGAAGATGCTGGGCAGAGTGACCTGCTCGCCAACTGA

Upstream 100 bases:

>100_bases
CAATCGCTTGAAAGGTCAACAACGCAACGGCTAGCGTTGGCATGTCGTCGTGGTTCGACACCGATCCCTCTCGACTCTGC
ATAACAACAAGGAGACATCC

Downstream 100 bases:

>100_bases
GGTTTTCTTCCTTCACTTCCTTGAGGCGCGTGTCGCGATCGGCAGGTAGTCGGTCGCGACACGCGGGATCATCATGTATC
GAGATACCTTGCTGAGACTA

Product: TRAP dicarboxylate transporter subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MRILTPRNVLLAAVTTGMMATVSLANAATTLNLSYNGPPDTDKNAVHLFASNLKRLVEEKTDGDIQLKLYPNSMLGEEQE
RMEQVINTPSLNIASFAGLSPIVPEIYVSAIPFLFEDYEAAHQFFDEGDYWNKVEDTLEERTGAELLGVIEEGGFLDFTN
SKRPISSPEDFEGLRFRAMDPSQVALYEAFGASGTPIPWTDTYMALKTNVADGQMNPPMYIIMGSLYEVQKYLTLANVQY
SDQFLIANGEWYDDLSEENRQAIEAAVQEASELNREDVEKRVDERIQFLADQGMEVIEPTEDELAAFREKGQPAYIEWLT
DEQGIDRAWIEMALEDAGQSDLLAN

Sequences:

>Translated_345_residues
MRILTPRNVLLAAVTTGMMATVSLANAATTLNLSYNGPPDTDKNAVHLFASNLKRLVEEKTDGDIQLKLYPNSMLGEEQE
RMEQVINTPSLNIASFAGLSPIVPEIYVSAIPFLFEDYEAAHQFFDEGDYWNKVEDTLEERTGAELLGVIEEGGFLDFTN
SKRPISSPEDFEGLRFRAMDPSQVALYEAFGASGTPIPWTDTYMALKTNVADGQMNPPMYIIMGSLYEVQKYLTLANVQY
SDQFLIANGEWYDDLSEENRQAIEAAVQEASELNREDVEKRVDERIQFLADQGMEVIEPTEDELAAFREKGQPAYIEWLT
DEQGIDRAWIEMALEDAGQSDLLAN
>Mature_345_residues
MRILTPRNVLLAAVTTGMMATVSLANAATTLNLSYNGPPDTDKNAVHLFASNLKRLVEEKTDGDIQLKLYPNSMLGEEQE
RMEQVINTPSLNIASFAGLSPIVPEIYVSAIPFLFEDYEAAHQFFDEGDYWNKVEDTLEERTGAELLGVIEEGGFLDFTN
SKRPISSPEDFEGLRFRAMDPSQVALYEAFGASGTPIPWTDTYMALKTNVADGQMNPPMYIIMGSLYEVQKYLTLANVQY
SDQFLIANGEWYDDLSEENRQAIEAAVQEASELNREDVEKRVDERIQFLADQGMEVIEPTEDELAAFREKGQPAYIEWLT
DEQGIDRAWIEMALEDAGQSDLLAN

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=298, Percent_Identity=32.2147651006711, Blast_Score=147, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 38700; Mature: 38700

Theoretical pI: Translated: 3.89; Mature: 3.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILTPRNVLLAAVTTGMMATVSLANAATTLNLSYNGPPDTDKNAVHLFASNLKRLVEEK
CCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHC
TDGDIQLKLYPNSMLGEEQERMEQVINTPSLNIASFAGLSPIVPEIYVSAIPFLFEDYEA
CCCCEEEEECCCHHCCCHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
AHQFFDEGDYWNKVEDTLEERTGAELLGVIEEGGFLDFTNSKRPISSPEDFEGLRFRAMD
HHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCEEEECC
PSQVALYEAFGASGTPIPWTDTYMALKTNVADGQMNPPMYIIMGSLYEVQKYLTLANVQY
CHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECHHHHHHHHHHHHCCEE
SDQFLIANGEWYDDLSEENRQAIEAAVQEASELNREDVEKRVDERIQFLADQGMEVIEPT
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHCCCC
EDELAAFREKGQPAYIEWLTDEQGIDRAWIEMALEDAGQSDLLAN
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRILTPRNVLLAAVTTGMMATVSLANAATTLNLSYNGPPDTDKNAVHLFASNLKRLVEEK
CCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHC
TDGDIQLKLYPNSMLGEEQERMEQVINTPSLNIASFAGLSPIVPEIYVSAIPFLFEDYEA
CCCCEEEEECCCHHCCCHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
AHQFFDEGDYWNKVEDTLEERTGAELLGVIEEGGFLDFTNSKRPISSPEDFEGLRFRAMD
HHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCEEEECC
PSQVALYEAFGASGTPIPWTDTYMALKTNVADGQMNPPMYIIMGSLYEVQKYLTLANVQY
CHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECHHHHHHHHHHHHCCEE
SDQFLIANGEWYDDLSEENRQAIEAAVQEASELNREDVEKRVDERIQFLADQGMEVIEPT
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHCCCC
EDELAAFREKGQPAYIEWLTDEQGIDRAWIEMALEDAGQSDLLAN
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]