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The map label for this gene is opuD [H]

Identifier: 92112786

GI number: 92112786

Start: 732371

End: 734005

Strand: Direct

Name: opuD [H]

Synonym: Csal_0654

Alternate gene names: 92112786

Gene position: 732371-734005 (Clockwise)

Preceding gene: 92112778

Following gene: 92112788

Centisome position: 19.81

GC content: 61.9

Gene sequence:

>1635_bases
ATGGCCAACAATGGAAGGCAACGCGTATTTATCGTCTCCGCCTTGATCGTCGCGGTTCTCGTCGCCATCGGGGCGGCGTT
TCCCGAGCGTTTCGGCAGCGCGGCGTCGTCGGCATTATCCGGCATTTCGCACTATTTCGGGTGGTTCTACCTGTTTTCGG
TGTTCGGGTTCGTGGTGTTCTTGCTGACGCTGGCGTGCAGCAAGTATGGCAAGATTCGGCTCGGCCCCCAGGACAGTTCG
CCCTCCTACAGTTTCTTCTCGTGGGTCAGCATGCTGCTGGCGGCGGGGTTCGGCGTCGGCCTGGTGTTCTACGGCATGGC
CGAGCCGATGACGCACTTCCTCGAACCGCCCTACGGTGATGTGGAAGGCGGTACCGAAGAGGCGGCACGCTATGCCATCC
AATACAGCTTCTTCAACTGGGGCATTCATCAGTGGGCCGCGTTTTCCGTGGTGGGGCTGATCATTGCCTATTATCAGTTC
CGCAAGGGCCAGGCGGGGCTGGTCTCCAACGTGCTTTCGAGCATGACCGCCAAGCGTCCCAAGATGCGCAAGCTGGGACC
GGCGCTGGATGTCTTTGCCGTGGTCGCGACGGTGATGGGGGTGGCGACCTCCATCGGTCTGGCGGTGCTGCAGATCAACG
GCGGCCTGCATGCGGTCTTCGGTGTCGAAGAGGGCATGACGTGGCAGTTCATCATCATGGGGGCGATGTTTTTGTGTTAC
ATGGCCTCCACCTGGTCGGGGCTGGACAAGGGCATCAAGCGCCTTTCCAACCTCAACATGGCGCTGTGCTTCGCGTTGAT
GTTCTACGTGCTGTTCACGGGCCCCACCGTGGCCATTCTCGAGACCATCACCCTGGGGATCGGCGATTACCTGCAGAACA
TCGTGGGCATGAGCCTGCGGGTCGCGCCGTATAGCGACAACACCTGGGCCAGCAACTGGACGATCTTCTATTGGGCCTGG
GTCATCGCCTGGTCGCCGTTCGTGGGCACCTTCGTGGCGCGCGTCTCGCGTGGGCGCACCATCAAGGAGTACGTGTTCGG
CGTGTTGATCGTGCCGCCGCTGCTGGCCTGCCTGTGGATCGGGGTCTTCGGCGGCGCGGCGCTCAACATGGAGCTCACCG
GCGACGTGGGACTGGCCTCGGCCACGGCAGACAACATCACGGTGGCGCTGTTCCGGATGTTCGAGCTGATGCCGTTCTCC
AATGTGCTGTCGGTGGTGGCGCTGTCGCTGATCTTCATTTTCCTGGTGACCTCGGCGGACTCGGCGACCTATATCGTGTC
GCAGATGACCGATGGCGGTTCGCTGAATCCGCCGCTGTTCAAGCGGGTGATCTGGGGGGTACTGATCGCGGCGATCTGTC
TGACCCTGCTGATTGCCGGCGGGTTGAATGGCCTGCAATCGGCGGCGGTGCTGGCGGCGTTACCCTTCACCTTCATCCTG
TACGGCATGATTGCCGTGCTGGTGAAGGAATTGCGCGCCGATCGCAAGGCGATGCTGACATCGCTTTATCATCGTCATGG
GGAAACGCCGGTAGGCGCCGATGCCTTCGAGGCGGAAACGCTGGCGGAAGCCGAGCGGTACCGGCGTGCACCGAACGTGG
TCAACCGGCGCATCAATACGCGCGACGGTACCTGA

Upstream 100 bases:

>100_bases
GCTTTGGTACTCACATGCATTGCAGACCTGCCGAGCACGGTGATAAGAAGCCCGAAAGGCATGGAATATCGCCTTGTGGG
TGACTCAAGAGGATAGCAGC

Downstream 100 bases:

>100_bases
GGAACGCGCTCGATGGTGGCGAACGCCCGGCTTCGGCCGGGCGTTTTGCGTCGTCCTCTGGTGGAATCGGACCTCAGCCC
TTATCGTTAGTCGGATCGTT

Product: choline/carnitine/betaine transport

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MANNGRQRVFIVSALIVAVLVAIGAAFPERFGSAASSALSGISHYFGWFYLFSVFGFVVFLLTLACSKYGKIRLGPQDSS
PSYSFFSWVSMLLAAGFGVGLVFYGMAEPMTHFLEPPYGDVEGGTEEAARYAIQYSFFNWGIHQWAAFSVVGLIIAYYQF
RKGQAGLVSNVLSSMTAKRPKMRKLGPALDVFAVVATVMGVATSIGLAVLQINGGLHAVFGVEEGMTWQFIIMGAMFLCY
MASTWSGLDKGIKRLSNLNMALCFALMFYVLFTGPTVAILETITLGIGDYLQNIVGMSLRVAPYSDNTWASNWTIFYWAW
VIAWSPFVGTFVARVSRGRTIKEYVFGVLIVPPLLACLWIGVFGGAALNMELTGDVGLASATADNITVALFRMFELMPFS
NVLSVVALSLIFIFLVTSADSATYIVSQMTDGGSLNPPLFKRVIWGVLIAAICLTLLIAGGLNGLQSAAVLAALPFTFIL
YGMIAVLVKELRADRKAMLTSLYHRHGETPVGADAFEAETLAEAERYRRAPNVVNRRINTRDGT

Sequences:

>Translated_544_residues
MANNGRQRVFIVSALIVAVLVAIGAAFPERFGSAASSALSGISHYFGWFYLFSVFGFVVFLLTLACSKYGKIRLGPQDSS
PSYSFFSWVSMLLAAGFGVGLVFYGMAEPMTHFLEPPYGDVEGGTEEAARYAIQYSFFNWGIHQWAAFSVVGLIIAYYQF
RKGQAGLVSNVLSSMTAKRPKMRKLGPALDVFAVVATVMGVATSIGLAVLQINGGLHAVFGVEEGMTWQFIIMGAMFLCY
MASTWSGLDKGIKRLSNLNMALCFALMFYVLFTGPTVAILETITLGIGDYLQNIVGMSLRVAPYSDNTWASNWTIFYWAW
VIAWSPFVGTFVARVSRGRTIKEYVFGVLIVPPLLACLWIGVFGGAALNMELTGDVGLASATADNITVALFRMFELMPFS
NVLSVVALSLIFIFLVTSADSATYIVSQMTDGGSLNPPLFKRVIWGVLIAAICLTLLIAGGLNGLQSAAVLAALPFTFIL
YGMIAVLVKELRADRKAMLTSLYHRHGETPVGADAFEAETLAEAERYRRAPNVVNRRINTRDGT
>Mature_543_residues
ANNGRQRVFIVSALIVAVLVAIGAAFPERFGSAASSALSGISHYFGWFYLFSVFGFVVFLLTLACSKYGKIRLGPQDSSP
SYSFFSWVSMLLAAGFGVGLVFYGMAEPMTHFLEPPYGDVEGGTEEAARYAIQYSFFNWGIHQWAAFSVVGLIIAYYQFR
KGQAGLVSNVLSSMTAKRPKMRKLGPALDVFAVVATVMGVATSIGLAVLQINGGLHAVFGVEEGMTWQFIIMGAMFLCYM
ASTWSGLDKGIKRLSNLNMALCFALMFYVLFTGPTVAILETITLGIGDYLQNIVGMSLRVAPYSDNTWASNWTIFYWAWV
IAWSPFVGTFVARVSRGRTIKEYVFGVLIVPPLLACLWIGVFGGAALNMELTGDVGLASATADNITVALFRMFELMPFSN
VLSVVALSLIFIFLVTSADSATYIVSQMTDGGSLNPPLFKRVIWGVLIAAICLTLLIAGGLNGLQSAAVLAALPFTFILY
GMIAVLVKELRADRKAMLTSLYHRHGETPVGADAFEAETLAEAERYRRAPNVVNRRINTRDGT

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=461, Percent_Identity=36.2255965292842, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI1788102, Length=489, Percent_Identity=30.4703476482618, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1786224, Length=510, Percent_Identity=25.2941176470588, Blast_Score=160, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 59142; Mature: 59011

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANNGRQRVFIVSALIVAVLVAIGAAFPERFGSAASSALSGISHYFGWFYLFSVFGFVVF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLTLACSKYGKIRLGPQDSSPSYSFFSWVSMLLAAGFGVGLVFYGMAEPMTHFLEPPYGD
HHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCC
VEGGTEEAARYAIQYSFFNWGIHQWAAFSVVGLIIAYYQFRKGQAGLVSNVLSSMTAKRP
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
KMRKLGPALDVFAVVATVMGVATSIGLAVLQINGGLHAVFGVEEGMTWQFIIMGAMFLCY
HHHHHCHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHH
MASTWSGLDKGIKRLSNLNMALCFALMFYVLFTGPTVAILETITLGIGDYLQNIVGMSLR
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEE
VAPYSDNTWASNWTIFYWAWVIAWSPFVGTFVARVSRGRTIKEYVFGVLIVPPLLACLWI
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GVFGGAALNMELTGDVGLASATADNITVALFRMFELMPFSNVLSVVALSLIFIFLVTSAD
HHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
SATYIVSQMTDGGSLNPPLFKRVIWGVLIAAICLTLLIAGGLNGLQSAAVLAALPFTFIL
CHHEEHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
YGMIAVLVKELRADRKAMLTSLYHRHGETPVGADAFEAETLAEAERYRRAPNVVNRRINT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCC
RDGT
CCCC
>Mature Secondary Structure 
ANNGRQRVFIVSALIVAVLVAIGAAFPERFGSAASSALSGISHYFGWFYLFSVFGFVVF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLTLACSKYGKIRLGPQDSSPSYSFFSWVSMLLAAGFGVGLVFYGMAEPMTHFLEPPYGD
HHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCC
VEGGTEEAARYAIQYSFFNWGIHQWAAFSVVGLIIAYYQFRKGQAGLVSNVLSSMTAKRP
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
KMRKLGPALDVFAVVATVMGVATSIGLAVLQINGGLHAVFGVEEGMTWQFIIMGAMFLCY
HHHHHCHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHH
MASTWSGLDKGIKRLSNLNMALCFALMFYVLFTGPTVAILETITLGIGDYLQNIVGMSLR
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEE
VAPYSDNTWASNWTIFYWAWVIAWSPFVGTFVARVSRGRTIKEYVFGVLIVPPLLACLWI
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GVFGGAALNMELTGDVGLASATADNITVALFRMFELMPFSNVLSVVALSLIFIFLVTSAD
HHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
SATYIVSQMTDGGSLNPPLFKRVIWGVLIAAICLTLLIAGGLNGLQSAAVLAALPFTFIL
CHHEEHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
YGMIAVLVKELRADRKAMLTSLYHRHGETPVGADAFEAETLAEAERYRRAPNVVNRRINT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCC
RDGT
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]