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The map label for this gene is eno

Identifier: 92112752

GI number: 92112752

Start: 693366

End: 694661

Strand: Direct

Name: eno

Synonym: Csal_0619

Alternate gene names: 92112752

Gene position: 693366-694661 (Clockwise)

Preceding gene: 92112751

Following gene: 92112755

Centisome position: 18.76

GC content: 65.2

Gene sequence:

>1296_bases
ATGGCTGAGATCAAAGACATCCACGCCCTCGAGGTGCTGGACTCACGCGGCAATCCGACCGTTCAGGCCGATGTGGTGCT
GGCCAGCGGCGTTCGCGGCACGGCCTGCGCGCCGAGCGGTGCCTCCACCGGCTCGCGCGAGGCCCTGGAGCTCCGCGACG
GCGATACGTCGCGCTATCTGGGCAAGGGCGTGCTCAAGGCAGTCGAGGCCGTCAACGGCAGGATTCGCGATGCCCTGGTA
GGCAAGGACGCCCTCGATCAGCGGGCCCTGGATGCCGCCATGCTCGAGCTCGACGGCACCGACAACAAGGCGGGGCTGGG
CGCCAATGCCATCCTCGCGGTGTCGCTGGCCGCCGCCAAGGCCGCCGCCATCGAGAAGGGCGTGCCGCTCTACGCGCACA
TCGCCGATCTGTACGGTCAGTCCGGCCAGTTCCGCATGCCGGTGCCGATGATGAATATCCTCAATGGCGGCGAGCACGCG
GACAACAACGTCGATATCCAGGAGTTCATGATCCAGCCGGTGGGGGCGCCCAGCTTCCGCGAGGCGCTGCGCATGGGCGC
CGAGATCTTCCATGCCCTCAAGAAGGTGCTCGCCGCGCGCGGCTTGTCGACCTCGGTGGGCGACGAGGGCGGCTTCGCGC
CCAATCTGGCATCCAACGCCGAGGCATTGGCGGTCATCCAGCAGGCGGTCGAGAAAGCCGGTTACGTGCTGGGCAAGGAT
GTGACCCTGGCGCTGGACTGTGCCTCGTCGGAGTTCTATCAGGACGGCCAGTACAACCTGTCGGGCGAAGGCAAGTCCTA
CGACGCAGCCGGCTTCGTCGATTACCTCGCGGCCCTTTGCGATCAGTATCCGATCGTCTCCATCGAAGATGGCATGGACG
AGTCCGACTGGGCAGGCTGGAAGGCCCTGACCGAGAAGCTGGGCGACAAGGTGCAGCTGGTCGGCGATGATCTGTTCGTG
ACCAACACGCGTATCCTCAAGCGCGGTATCGACGAGCACATCGGCAATTCCATCCTCATCAAGTTCAACCAGATCGGCTC
GTTGTCCGAGACGCTGGATGCCATCAAGATGGCGCAGGACGCCGGTTTCACCGCCGTCATTTCGCATCGCAGTGGCGAGA
CCGAAGATACCACCATCGCCGACCTGGCCGTGGGCACTTCCGCCGGCCAGATCAAGACCGGCTCGCTCTGTCGCAGCGAC
CGCGTGGCCAAGTACAACCGTCTGCTGGTCATCGAGCAGGAGCTGGAAGGCCAGGCCGTGTATCCGGGGCTGGCGGCGAT
CAAGGGCCAGGGCTGA

Upstream 100 bases:

>100_bases
AGTCGAGTACACGACGAGCGGAGCCGCCCGTGGCGGTCCATGGACCGCCGCCCTCTCGATATGTTGAAACGACCCATCGT
TAACACGAGGATGACTCGCA

Downstream 100 bases:

>100_bases
CGTCCTCGGGGTGCTCAGCACGGTATGAACGCCACGGCGGCCCTGAGGGCCGCCGTTTTGTTTGGTGCCTCGTGCGACGC
TTCCCTCCCGTGGTGGTCGT

Product: enolase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MAEIKDIHALEVLDSRGNPTVQADVVLASGVRGTACAPSGASTGSREALELRDGDTSRYLGKGVLKAVEAVNGRIRDALV
GKDALDQRALDAAMLELDGTDNKAGLGANAILAVSLAAAKAAAIEKGVPLYAHIADLYGQSGQFRMPVPMMNILNGGEHA
DNNVDIQEFMIQPVGAPSFREALRMGAEIFHALKKVLAARGLSTSVGDEGGFAPNLASNAEALAVIQQAVEKAGYVLGKD
VTLALDCASSEFYQDGQYNLSGEGKSYDAAGFVDYLAALCDQYPIVSIEDGMDESDWAGWKALTEKLGDKVQLVGDDLFV
TNTRILKRGIDEHIGNSILIKFNQIGSLSETLDAIKMAQDAGFTAVISHRSGETEDTTIADLAVGTSAGQIKTGSLCRSD
RVAKYNRLLVIEQELEGQAVYPGLAAIKGQG

Sequences:

>Translated_431_residues
MAEIKDIHALEVLDSRGNPTVQADVVLASGVRGTACAPSGASTGSREALELRDGDTSRYLGKGVLKAVEAVNGRIRDALV
GKDALDQRALDAAMLELDGTDNKAGLGANAILAVSLAAAKAAAIEKGVPLYAHIADLYGQSGQFRMPVPMMNILNGGEHA
DNNVDIQEFMIQPVGAPSFREALRMGAEIFHALKKVLAARGLSTSVGDEGGFAPNLASNAEALAVIQQAVEKAGYVLGKD
VTLALDCASSEFYQDGQYNLSGEGKSYDAAGFVDYLAALCDQYPIVSIEDGMDESDWAGWKALTEKLGDKVQLVGDDLFV
TNTRILKRGIDEHIGNSILIKFNQIGSLSETLDAIKMAQDAGFTAVISHRSGETEDTTIADLAVGTSAGQIKTGSLCRSD
RVAKYNRLLVIEQELEGQAVYPGLAAIKGQG
>Mature_430_residues
AEIKDIHALEVLDSRGNPTVQADVVLASGVRGTACAPSGASTGSREALELRDGDTSRYLGKGVLKAVEAVNGRIRDALVG
KDALDQRALDAAMLELDGTDNKAGLGANAILAVSLAAAKAAAIEKGVPLYAHIADLYGQSGQFRMPVPMMNILNGGEHAD
NNVDIQEFMIQPVGAPSFREALRMGAEIFHALKKVLAARGLSTSVGDEGGFAPNLASNAEALAVIQQAVEKAGYVLGKDV
TLALDCASSEFYQDGQYNLSGEGKSYDAAGFVDYLAALCDQYPIVSIEDGMDESDWAGWKALTEKLGDKVQLVGDDLFVT
NTRILKRGIDEHIGNSILIKFNQIGSLSETLDAIKMAQDAGFTAVISHRSGETEDTTIADLAVGTSAGQIKTGSLCRSDR
VAKYNRLLVIEQELEGQAVYPGLAAIKGQG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=52.5581395348837, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=51.1627906976744, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI301897477, Length=430, Percent_Identity=51.3953488372093, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI301897469, Length=430, Percent_Identity=51.3953488372093, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=46.7289719626168, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=27.9761904761905, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=27.9761904761905, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=27.9761904761905, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI310129182, Length=207, Percent_Identity=25.6038647342995, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI310110045, Length=207, Percent_Identity=25.6038647342995, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI310120572, Length=207, Percent_Identity=25.6038647342995, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1789141, Length=431, Percent_Identity=70.0696055684455, Blast_Score=597, Evalue=1e-172,
Organism=Caenorhabditis elegans, GI71995829, Length=430, Percent_Identity=50.9302325581395, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17536383, Length=430, Percent_Identity=50.9302325581395, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32563855, Length=189, Percent_Identity=44.973544973545, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6321693, Length=431, Percent_Identity=48.9559164733179, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=47.4537037037037, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=47.2222222222222, Blast_Score=372, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=47.2222222222222, Blast_Score=372, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6321968, Length=431, Percent_Identity=48.7238979118329, Blast_Score=353, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580918, Length=437, Percent_Identity=49.8855835240275, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580916, Length=437, Percent_Identity=49.8855835240275, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580920, Length=437, Percent_Identity=49.8855835240275, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580914, Length=437, Percent_Identity=49.8855835240275, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI281360527, Length=437, Percent_Identity=49.8855835240275, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17137654, Length=437, Percent_Identity=49.8855835240275, Blast_Score=381, Evalue=1e-106,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_CHRSD (Q1QZX7)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_572680.1
- ProteinModelPortal:   Q1QZX7
- SMR:   Q1QZX7
- STRING:   Q1QZX7
- GeneID:   4029088
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_0619
- NMPDR:   fig|290398.4.peg.422
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- BioCyc:   CSAL290398:CSAL_0619-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45375; Mature: 45243

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 209-209 ACT_SITE 341-341 BINDING 159-159 BINDING 168-168 BINDING 289-289 BINDING 316-316 BINDING 341-341 BINDING 392-392

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIKDIHALEVLDSRGNPTVQADVVLASGVRGTACAPSGASTGSREALELRDGDTSRYL
CCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVEAVNGRIRDALVGKDALDQRALDAAMLELDGTDNKAGLGANAILAVSLAAAK
HHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHH
AAAIEKGVPLYAHIADLYGQSGQFRMPVPMMNILNGGEHADNNVDIQEFMIQPVGAPSFR
HHHHHCCCCHHHHHHHHHCCCCCEECCCHHHHHHCCCCCCCCCCCHHHHHHCCCCCHHHH
EALRMGAEIFHALKKVLAARGLSTSVGDEGGFAPNLASNAEALAVIQQAVEKAGYVLGKD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCC
VTLALDCASSEFYQDGQYNLSGEGKSYDAAGFVDYLAALCDQYPIVSIEDGMDESDWAGW
EEEEEECCCCHHHCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHH
KALTEKLGDKVQLVGDDLFVTNTRILKRGIDEHIGNSILIKFNQIGSLSETLDAIKMAQD
HHHHHHCCCEEEEECCCEEEEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHC
AGFTAVISHRSGETEDTTIADLAVGTSAGQIKTGSLCRSDRVAKYNRLLVIEQELEGQAV
CCCEEEEECCCCCCCCCEEEHEEECCCCCCEECCCCCCCCCHHHHCEEEEEEECCCCCEE
YPGLAAIKGQG
CCCHHEECCCC
>Mature Secondary Structure 
AEIKDIHALEVLDSRGNPTVQADVVLASGVRGTACAPSGASTGSREALELRDGDTSRYL
CCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVEAVNGRIRDALVGKDALDQRALDAAMLELDGTDNKAGLGANAILAVSLAAAK
HHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHH
AAAIEKGVPLYAHIADLYGQSGQFRMPVPMMNILNGGEHADNNVDIQEFMIQPVGAPSFR
HHHHHCCCCHHHHHHHHHCCCCCEECCCHHHHHHCCCCCCCCCCCHHHHHHCCCCCHHHH
EALRMGAEIFHALKKVLAARGLSTSVGDEGGFAPNLASNAEALAVIQQAVEKAGYVLGKD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCC
VTLALDCASSEFYQDGQYNLSGEGKSYDAAGFVDYLAALCDQYPIVSIEDGMDESDWAGW
EEEEEECCCCHHHCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHH
KALTEKLGDKVQLVGDDLFVTNTRILKRGIDEHIGNSILIKFNQIGSLSETLDAIKMAQD
HHHHHHCCCEEEEECCCEEEEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHC
AGFTAVISHRSGETEDTTIADLAVGTSAGQIKTGSLCRSDRVAKYNRLLVIEQELEGQAV
CCCEEEEECCCCCCCCCEEEHEEECCCCCCEECCCCCCCCCHHHHCEEEEEEECCCCCEE
YPGLAAIKGQG
CCCHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA