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The map label for this gene is maeB [H]

Identifier: 92112738

GI number: 92112738

Start: 672559

End: 673857

Strand: Direct

Name: maeB [H]

Synonym: Csal_0605

Alternate gene names: 92112738

Gene position: 672559-673857 (Clockwise)

Preceding gene: 92112737

Following gene: 92112741

Centisome position: 18.19

GC content: 63.43

Gene sequence:

>1299_bases
ATGCAGATGACTGGACAAAAGCTCATGAGTGACGAAAAAAGACAAGCGGCGCTGGATTATCATGCCAAGCCGATTCCCGG
CAAGTTATCCGTCGAGTTGACCAAGCCGACCGAGACATCGAAGCACCTGGCGCTGGCGTATAGCCCCGGCGTGGCCGAAC
CCTGCCGCGAGATCGCCAAGGACGTCGAGAACGCGTACCTGTATACCGGCAAGGGCAATCTGGTCGCCGTGGTGTCCGAT
GGCAGCGCGATCCTGGGGCTGGGCAATCTGGGGCCGCTGTCCAGCAAGCCGGTCATGGAAGGCAAGGGCGTCCTGTTCAA
GCGCTTTGCCGGCATCAATTCCGTGGACATCGAAGTCGATGCCGAAAGCCCGCAGGCCTTCATCGACACCGTGGCGCGTA
TCGCCGATACCTGGGGCGGCATCAACCTCGAGGATATCAAGGCGCCGGAGTGCTTCGAGATCGAGCAGGCGTTGATCGAG
CAGTGCAACATTCCGGTGTTTCACGACGATCAGCACGGCACGGCCATCGTCACGGCCGCGGGGCTGCTCAATGCACTGGA
TATCGCCGGCAAGCGCTTGGAAGACGCCAGGATCGTGTGCCTGGGGGCAGGGGCGGCGGCCATCGCCTGCATGAAGCTGC
TGGTCTCGTGCGGTGCGCGTGCCGAGAATATCTTCATGCTCGACCGCAAGGGCGTCGTGCACAGCGAGCGCGATGACCTC
AACCAGTACAAGGCGATGTTCGCCACCGAGACGTCGCGCCGCACGCTGGACGACGCCATCGAAGGGGCCGATGTCTTCGT
GGGTCTCTCCGGGCCCAACCTGCTCAAGCCCGAGCAGCTCCAGCGCATGGCCGCGAACCCGGTGGTCTTCGCCTGCTCCA
ATCCGGACCCGGAAATCCACCCGGACGTCGCCAACGAAACGCGTGATGACGTGATCATGGCCACCGGTCGTTCCGACTAC
CCCAACCAGGTCAACAACGTGCTGGGCTTCCCGTTCATCTTCCGCGGGGCCCTGGATGTGCGCGCCACGCGCATCAACGA
GGAGATGAAAGTGGCGGCTGTGCATGCCTTGAAGGATCTGGCGCGCGAGCCGGTGACTCGCGAGGTACTGGATGGCTATG
GCATCGAGTCGCTCGAGTTCGGGCGGGGCTATATCATTCCCACGCCGCTGGACTCGCGGTTGCTCGATCGTGTCTCCTCG
GCGGTGGCGCAGGCGGCGGTGGACACCGGCGTGGCCCGCAAGCCCTATCCGGCGCACTACCCGCTGACCAGCATCAAGGA
CGTCTACGGTACGCCGTAA

Upstream 100 bases:

>100_bases
GGTAAATGCTTCAAACGATCGCTTAAAAGTCTTTAATGTCGCGAACTTGGCATAAGTTGTTCGGTGGAACGTTTTTCTAT
ATTCTGGCGGTCACGCCTCC

Downstream 100 bases:

>100_bases
ACCGCCTTGTCAGACGCCTGCGCTCGCAAGATGCGAGGCGCCAGGAACGCACGGGCCGCTCCATGGAGCGGCCCGTGCGT
CGTTGTGGCAGTGGCGAAGT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MQMTGQKLMSDEKRQAALDYHAKPIPGKLSVELTKPTETSKHLALAYSPGVAEPCREIAKDVENAYLYTGKGNLVAVVSD
GSAILGLGNLGPLSSKPVMEGKGVLFKRFAGINSVDIEVDAESPQAFIDTVARIADTWGGINLEDIKAPECFEIEQALIE
QCNIPVFHDDQHGTAIVTAAGLLNALDIAGKRLEDARIVCLGAGAAAIACMKLLVSCGARAENIFMLDRKGVVHSERDDL
NQYKAMFATETSRRTLDDAIEGADVFVGLSGPNLLKPEQLQRMAANPVVFACSNPDPEIHPDVANETRDDVIMATGRSDY
PNQVNNVLGFPFIFRGALDVRATRINEEMKVAAVHALKDLAREPVTREVLDGYGIESLEFGRGYIIPTPLDSRLLDRVSS
AVAQAAVDTGVARKPYPAHYPLTSIKDVYGTP

Sequences:

>Translated_432_residues
MQMTGQKLMSDEKRQAALDYHAKPIPGKLSVELTKPTETSKHLALAYSPGVAEPCREIAKDVENAYLYTGKGNLVAVVSD
GSAILGLGNLGPLSSKPVMEGKGVLFKRFAGINSVDIEVDAESPQAFIDTVARIADTWGGINLEDIKAPECFEIEQALIE
QCNIPVFHDDQHGTAIVTAAGLLNALDIAGKRLEDARIVCLGAGAAAIACMKLLVSCGARAENIFMLDRKGVVHSERDDL
NQYKAMFATETSRRTLDDAIEGADVFVGLSGPNLLKPEQLQRMAANPVVFACSNPDPEIHPDVANETRDDVIMATGRSDY
PNQVNNVLGFPFIFRGALDVRATRINEEMKVAAVHALKDLAREPVTREVLDGYGIESLEFGRGYIIPTPLDSRLLDRVSS
AVAQAAVDTGVARKPYPAHYPLTSIKDVYGTP
>Mature_432_residues
MQMTGQKLMSDEKRQAALDYHAKPIPGKLSVELTKPTETSKHLALAYSPGVAEPCREIAKDVENAYLYTGKGNLVAVVSD
GSAILGLGNLGPLSSKPVMEGKGVLFKRFAGINSVDIEVDAESPQAFIDTVARIADTWGGINLEDIKAPECFEIEQALIE
QCNIPVFHDDQHGTAIVTAAGLLNALDIAGKRLEDARIVCLGAGAAAIACMKLLVSCGARAENIFMLDRKGVVHSERDDL
NQYKAMFATETSRRTLDDAIEGADVFVGLSGPNLLKPEQLQRMAANPVVFACSNPDPEIHPDVANETRDDVIMATGRSDY
PNQVNNVLGFPFIFRGALDVRATRINEEMKVAAVHALKDLAREPVTREVLDGYGIESLEFGRGYIIPTPLDSRLLDRVSS
AVAQAAVDTGVARKPYPAHYPLTSIKDVYGTP

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=398, Percent_Identity=26.1306532663317, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1788806, Length=409, Percent_Identity=58.1907090464548, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI87081919, Length=286, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17537199, Length=360, Percent_Identity=26.1111111111111, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322823, Length=232, Percent_Identity=28.0172413793103, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI78707242, Length=353, Percent_Identity=27.7620396600567, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI78707238, Length=353, Percent_Identity=27.7620396600567, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI78707240, Length=353, Percent_Identity=27.7620396600567, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281362672, Length=458, Percent_Identity=26.4192139737991, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281362674, Length=458, Percent_Identity=26.4192139737991, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21356279, Length=458, Percent_Identity=26.4192139737991, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24646388, Length=397, Percent_Identity=25.6926952141058, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24646386, Length=397, Percent_Identity=25.6926952141058, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 46600; Mature: 46600

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMTGQKLMSDEKRQAALDYHAKPIPGKLSVELTKPTETSKHLALAYSPGVAEPCREIAK
CCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHH
DVENAYLYTGKGNLVAVVSDGSAILGLGNLGPLSSKPVMEGKGVLFKRFAGINSVDIEVD
HHCCCEEEECCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEE
AESPQAFIDTVARIADTWGGINLEDIKAPECFEIEQALIEQCNIPVFHDDQHGTAIVTAA
CCCCHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEHH
GLLNALDIAGKRLEDARIVCLGAGAAAIACMKLLVSCGARAENIFMLDRKGVVHSERDDL
HHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHH
NQYKAMFATETSRRTLDDAIEGADVFVGLSGPNLLKPEQLQRMAANPVVFACSNPDPEIH
HHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCEEEEECCCCCCCC
PDVANETRDDVIMATGRSDYPNQVNNVLGFPFIFRGALDVRATRINEEMKVAAVHALKDL
CCCCCCCCCCEEEEECCCCCCHHHHHHCCCHHHHHCCHHHHHHHCCHHHHHHHHHHHHHH
AREPVTREVLDGYGIESLEFGRGYIIPTPLDSRLLDRVSSAVAQAAVDTGVARKPYPAHY
HHCCHHHHHHHCCCCCEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PLTSIKDVYGTP
CHHHHHHHCCCC
>Mature Secondary Structure
MQMTGQKLMSDEKRQAALDYHAKPIPGKLSVELTKPTETSKHLALAYSPGVAEPCREIAK
CCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHH
DVENAYLYTGKGNLVAVVSDGSAILGLGNLGPLSSKPVMEGKGVLFKRFAGINSVDIEVD
HHCCCEEEECCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEE
AESPQAFIDTVARIADTWGGINLEDIKAPECFEIEQALIEQCNIPVFHDDQHGTAIVTAA
CCCCHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEHH
GLLNALDIAGKRLEDARIVCLGAGAAAIACMKLLVSCGARAENIFMLDRKGVVHSERDDL
HHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHH
NQYKAMFATETSRRTLDDAIEGADVFVGLSGPNLLKPEQLQRMAANPVVFACSNPDPEIH
HHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCEEEEECCCCCCCC
PDVANETRDDVIMATGRSDYPNQVNNVLGFPFIFRGALDVRATRINEEMKVAAVHALKDL
CCCCCCCCCCEEEEECCCCCCHHHHHHCCCHHHHHCCHHHHHHHCCHHHHHHHHHHHHHH
AREPVTREVLDGYGIESLEFGRGYIIPTPLDSRLLDRVSSAVAQAAVDTGVARKPYPAHY
HHCCHHHHHHHCCCCCEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PLTSIKDVYGTP
CHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]