Click here to switch to the map view.

The map label for this gene is tilS [H]

Identifier: 92112713

GI number: 92112713

Start: 644991

End: 646283

Strand: Direct

Name: tilS [H]

Synonym: Csal_0580

Alternate gene names: 92112713

Gene position: 644991-646283 (Clockwise)

Preceding gene: 92112712

Following gene: 92112715

Centisome position: 17.45

GC content: 72.08

Gene sequence:

>1293_bases
ATGGCGCGCCCCAATAACGCATCGTCCTCCCCCGACGCCCTTGTCGTTGACGCCCTGGCGATGACCTCGCCGGGGCGTTG
CGTTTGGGTGGCCCTTTCCGGCGGACTGGATTCGTCGCTGCTGCTGACACTCGCAGTCGCCGCCTGCCGCCGCCATCCGC
GACCGCTCTATGCGGTGCACGTCAATCACGGCCTGCAGGACGCCGCTGCCGACTTCGAGGCGCATGCGCGGTGGCTGTGC
GGCCGTCTCGGGGTGCCGTTGTTCGTGGAGCGCGCCAGGGTCGAGCGCGGTGCACGGGGACTCGAAGCTCACGCCCGCGA
TGCTCGGCTGGCGGCCTTTGCCAGGCGCGTCGCGCCGGGCGAGACGCTATTGCTTGCCCAGCACGCCGACGACCAGGCGG
AGACCCTGTTGCTGGCGGGACTGCGAGGCAGCGGCTTGCGCGGCATGGCCGCCATGCCCGAAAGGCGCGAATGGCAGGGG
CGTGTCATGGTCCGGCCCTGGCTGTCCGTGACGCGGGCGGCGCTCGCGCGCGAGGCCGAGGCGCGCGGCCTGCGCTGGAT
CGACGATCCGAGCAACGCCGACGAACGCTTCGACCGCAACTATCTGCGGCGTAGCGTCATGCCGGCGCTGGCGTCACGCT
GGCCGGAGGCGGCGACGTCGCTGTCACGCACCGCCGAGCAGCTGGGCGAGAGCGATGCGTTACTGGAAGAGCTGGCGGGG
GAAACCTTGCAGGCCCTGGGCGGCGACGCCGCCTGCTTGCCGGTGGCGGATCTCCTGGCGCTGTCCCGCCCACGCCAGCG
GCTTCTCGTGCGCCACGCCTGTCGGCAACTCTCTCTGCCCACGCCGCCGCGGGCGCGTCTCGAGAGCCTGCTCGAACAGC
TCGAGGCGCGCGGCGACGCACAGATCCATGTCGACTGGCCGGGCGGGGAAGCCCGGCGCTGGCAAGGGCACCTCCATCTG
CTGGCGCCGCGCGAGCCTGTCGATGTCGACTGGCAAGCCGTGTGGGATGGCGTCACTCCTCTGGAAACGCCGTCGGGCCC
TCTGGACTGGCAGCTGGTAGCCGAGTCGCCGCGTGATGTCCGTCTCACGCTGGGCCGGCGACGGGGCGGCGAGACGCTGC
GCGTGGCGGGACGCGGCCGGCGCGACGTCAAGCGGCTTCTGCAGGAAGCCGGGATTCCCCCCTGGCAGCGCGAACGCGTA
TGGCTGGTCTGGGAGGGCGAGACCCTGGTCGGGGTACTGGGCATCGCCGCCGCCGAGGGCTGGCGGCTGCGGGCGATGCC
GACTAGCGGCTGA

Upstream 100 bases:

>100_bases
GCCGGTCATATCAAGGACGCGCTGGGTGCCAGCCTCGAGCGACTGGAGTCCATGGAAATGGACGATCTGCTGTCGCGCCG
TTACGAGCGCCTGATGAGTT

Downstream 100 bases:

>100_bases
GCTCGAGCCGAAACCCGGCGTTGCGCTGATAGAGCGCTTCCTGTTCCAGGTCGGCGCTCAGCAGGGGCTGATCGCTGGCC
TGGTCGAGGTCCACGTAGAG

Product: tRNA(Ile)-lysidine synthetase-like protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MARPNNASSSPDALVVDALAMTSPGRCVWVALSGGLDSSLLLTLAVAACRRHPRPLYAVHVNHGLQDAAADFEAHARWLC
GRLGVPLFVERARVERGARGLEAHARDARLAAFARRVAPGETLLLAQHADDQAETLLLAGLRGSGLRGMAAMPERREWQG
RVMVRPWLSVTRAALAREAEARGLRWIDDPSNADERFDRNYLRRSVMPALASRWPEAATSLSRTAEQLGESDALLEELAG
ETLQALGGDAACLPVADLLALSRPRQRLLVRHACRQLSLPTPPRARLESLLEQLEARGDAQIHVDWPGGEARRWQGHLHL
LAPREPVDVDWQAVWDGVTPLETPSGPLDWQLVAESPRDVRLTLGRRRGGETLRVAGRGRRDVKRLLQEAGIPPWQRERV
WLVWEGETLVGVLGIAAAEGWRLRAMPTSG

Sequences:

>Translated_430_residues
MARPNNASSSPDALVVDALAMTSPGRCVWVALSGGLDSSLLLTLAVAACRRHPRPLYAVHVNHGLQDAAADFEAHARWLC
GRLGVPLFVERARVERGARGLEAHARDARLAAFARRVAPGETLLLAQHADDQAETLLLAGLRGSGLRGMAAMPERREWQG
RVMVRPWLSVTRAALAREAEARGLRWIDDPSNADERFDRNYLRRSVMPALASRWPEAATSLSRTAEQLGESDALLEELAG
ETLQALGGDAACLPVADLLALSRPRQRLLVRHACRQLSLPTPPRARLESLLEQLEARGDAQIHVDWPGGEARRWQGHLHL
LAPREPVDVDWQAVWDGVTPLETPSGPLDWQLVAESPRDVRLTLGRRRGGETLRVAGRGRRDVKRLLQEAGIPPWQRERV
WLVWEGETLVGVLGIAAAEGWRLRAMPTSG
>Mature_429_residues
ARPNNASSSPDALVVDALAMTSPGRCVWVALSGGLDSSLLLTLAVAACRRHPRPLYAVHVNHGLQDAAADFEAHARWLCG
RLGVPLFVERARVERGARGLEAHARDARLAAFARRVAPGETLLLAQHADDQAETLLLAGLRGSGLRGMAAMPERREWQGR
VMVRPWLSVTRAALAREAEARGLRWIDDPSNADERFDRNYLRRSVMPALASRWPEAATSLSRTAEQLGESDALLEELAGE
TLQALGGDAACLPVADLLALSRPRQRLLVRHACRQLSLPTPPRARLESLLEQLEARGDAQIHVDWPGGEARRWQGHLHLL
APREPVDVDWQAVWDGVTPLETPSGPLDWQLVAESPRDVRLTLGRRRGGETLRVAGRGRRDVKRLLQEAGIPPWQRERVW
LVWEGETLVGVLGIAAAEGWRLRAMPTSG

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=404, Percent_Identity=37.8712871287129, Blast_Score=208, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 47291; Mature: 47159

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPNNASSSPDALVVDALAMTSPGRCVWVALSGGLDSSLLLTLAVAACRRHPRPLYAVH
CCCCCCCCCCCCCEEEHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEE
VNHGLQDAAADFEAHARWLCGRLGVPLFVERARVERGARGLEAHARDARLAAFARRVAPG
ECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
ETLLLAQHADDQAETLLLAGLRGSGLRGMAAMPERREWQGRVMVRPWLSVTRAALAREAE
CEEEEEECCCCHHHEEEEECCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHH
ARGLRWIDDPSNADERFDRNYLRRSVMPALASRWPEAATSLSRTAEQLGESDALLEELAG
HCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHH
ETLQALGGDAACLPVADLLALSRPRQRLLVRHACRQLSLPTPPRARLESLLEQLEARGDA
HHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
QIHVDWPGGEARRWQGHLHLLAPREPVDVDWQAVWDGVTPLETPSGPLDWQLVAESPRDV
EEEEECCCCCCCEECCCEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEECCCCCE
RLTLGRRRGGETLRVAGRGRRDVKRLLQEAGIPPWQRERVWLVWEGETLVGVLGIAAAEG
EEEECCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEHHHHHHHCCC
WRLRAMPTSG
CEEEECCCCC
>Mature Secondary Structure 
ARPNNASSSPDALVVDALAMTSPGRCVWVALSGGLDSSLLLTLAVAACRRHPRPLYAVH
CCCCCCCCCCCCEEEHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEE
VNHGLQDAAADFEAHARWLCGRLGVPLFVERARVERGARGLEAHARDARLAAFARRVAPG
ECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
ETLLLAQHADDQAETLLLAGLRGSGLRGMAAMPERREWQGRVMVRPWLSVTRAALAREAE
CEEEEEECCCCHHHEEEEECCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHH
ARGLRWIDDPSNADERFDRNYLRRSVMPALASRWPEAATSLSRTAEQLGESDALLEELAG
HCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHH
ETLQALGGDAACLPVADLLALSRPRQRLLVRHACRQLSLPTPPRARLESLLEQLEARGDA
HHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
QIHVDWPGGEARRWQGHLHLLAPREPVDVDWQAVWDGVTPLETPSGPLDWQLVAESPRDV
EEEEECCCCCCCEECCCEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEECCCCCE
RLTLGRRRGGETLRVAGRGRRDVKRLLQEAGIPPWQRERVWLVWEGETLVGVLGIAAAEG
EEEECCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEHHHHHHHCCC
WRLRAMPTSG
CEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]