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The map label for this gene is ilvI [H]

Identifier: 92112634

GI number: 92112634

Start: 551389

End: 553146

Strand: Direct

Name: ilvI [H]

Synonym: Csal_0501

Alternate gene names: 92112634

Gene position: 551389-553146 (Clockwise)

Preceding gene: 92112632

Following gene: 92112635

Centisome position: 14.92

GC content: 61.89

Gene sequence:

>1758_bases
ATGGCACTCGCTATCTCCAGGGGAGAACTACCAGTGGAATTGCTTTCCGGCGCCGATATGATCGCCCGCTTCCTGCAGGA
TGAGGGCGTCGAGCGTATCTATGGCTATCCCGGCGGCGCGGCGTTGCACATCTATGACGCGCTGTTTCGCCAGGACAAGG
TCAAACACATTCTGGTTCGCCATGAGCAGGCCGCCACTCACGCCGCCGATGGCTATGCCCGTGCCTCGGGCAAGCCGGGC
ACGGTGCTGGTCACGTCCGGCCCCGGGGCGACCAATGCCGTGACCGGCATTGCCACCGCCTACATGGACTCGATTCCCAT
GGTCGTGCTGTGTGGCCAGGTGGCCAGCCACCTGATCGGTGAAGACGCCTTCCAGGAAACCGATATCGTGGGCGTGACAC
GGCCCATCGTGAAGCACAGCTTCGCCATCAAGCATCCCTCGGAAATTCCCGAGGTGCTCAAGAAGGCCTATTACCTCGCC
TCGACCGGTCGTCCCGGGCCGGTGGTCGTGGACATTCCCAAGGATATGACGGCGCCCACCGAGCGTTATGAGTACGTCTA
TCCGAAGAAGGTCAAGATTCGCTCCTACAACCCGGTCACGCGGGGGCACAGCGGCCAGATCAAGAAGGCCGTGGACATGA
TGCTCAAGGCACGGCGTCCGGTGTTCTATACCGGGGGTGGCATCGTCACCAGCGGCGCCAGCGAGAACCTGACCGCACTG
GTGCGTCGCCTGGGCTTCCCGATCACCACCACGCTGATGGGGATCGGCGCCTATCCGCAGACCGATGCGCAATCGCTGAG
CTGGCTGGGCATGCATGGCAGCTACGAGGCCAACATGGCCATGCACCATGCCGATCTGATCATCGCCATCGGCGCGCGCT
TCGATGATCGCGTGACCAACAATACGTCCAAGTTCTGCCCCACCGCCAAGATCGTGCACGTGGATATCGACCCCAGCTCG
ATTTCCAAGACGGTGCGCGCCGATGTGCCGATCGTGGGGCCGGCAGACAGCGTGATCAATGAGATGATCAGCCTGCTGCA
AGGTCGTGAAATGGCCAATGCGGAAGCCCTGCCGGAGTGGTGGGCGACCATCGACGGCTGGCGCGCCGAGCGCGAGGGCA
AGCTCTACGAGCCGTCCCAGCCGGGCGAACGCCTCAAACCGCAGGAGGTCGTCGAGGCGGTGTATCACATCACGCGCGGC
GAGGCGTTCGTGACCACCGACGTCGGCCAGCACCAGATGTTCGCGGCACAGTACTACAAGTTCGACAAGCCCAACCGCTG
GATCACGTCCGGGGGCCTGGGCACGATGGGCTTCGGGTTTCCGGCGGCGATGGGCATCAAGCAGAACTTCCCCGACGACA
TCGTCGTCTGCTTCACGGGGGAAGGCAGCTTTCAGATGATGATGCAGGAGTTGTCGACCTGTAAGCAGTTCGGCACCGGA
GTGAAGATCATCAATCTCAACAACGGGTCGCTGGGCATGGTGCGCCAGTGGCAGGATCTCAACTACAAGTCGCGGCATGC
GCATTCCTACATGGAATCGCTGCCGGATTTCCACAAGTTGATCGAAGCCTACGGCTTCACGGCGCTCAAGGTGGAACGCC
ACGACCAGCTCGACGCGGCACTGGAACGCATGTTCGCCGACAACGACGAACTGGTGTTCCTGGATGTCGATGTCGATCCT
CGTGAACACGTCTACCCGATGCAGGTGCCTTTGGGCGCCATGCGTGACATGCTGCTTTCGAAGACGGAGCGGACCTGA

Upstream 100 bases:

>100_bases
TCCGGGCCGAAGGACGCCAGGGCATGGCATCAGGTGCCGGCGCAGCCGGCAGCGGCGTACCGCCGCATCGACACTTCGTC
GTCCGTAACGTGACGGCGAA

Downstream 100 bases:

>100_bases
TGCGCCACATCATCTCGATCCTGCTGGAAAACGAACCCGGCGCCCTGTCCCGCGTGGTGGGCCTGTTTTCGCAGCGCAAC
TTCAACATCGAAACCCTCAA

Product: acetolactate synthase, large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MALAISRGELPVELLSGADMIARFLQDEGVERIYGYPGGAALHIYDALFRQDKVKHILVRHEQAATHAADGYARASGKPG
TVLVTSGPGATNAVTGIATAYMDSIPMVVLCGQVASHLIGEDAFQETDIVGVTRPIVKHSFAIKHPSEIPEVLKKAYYLA
STGRPGPVVVDIPKDMTAPTERYEYVYPKKVKIRSYNPVTRGHSGQIKKAVDMMLKARRPVFYTGGGIVTSGASENLTAL
VRRLGFPITTTLMGIGAYPQTDAQSLSWLGMHGSYEANMAMHHADLIIAIGARFDDRVTNNTSKFCPTAKIVHVDIDPSS
ISKTVRADVPIVGPADSVINEMISLLQGREMANAEALPEWWATIDGWRAEREGKLYEPSQPGERLKPQEVVEAVYHITRG
EAFVTTDVGQHQMFAAQYYKFDKPNRWITSGGLGTMGFGFPAAMGIKQNFPDDIVVCFTGEGSFQMMMQELSTCKQFGTG
VKIINLNNGSLGMVRQWQDLNYKSRHAHSYMESLPDFHKLIEAYGFTALKVERHDQLDAALERMFADNDELVFLDVDVDP
REHVYPMQVPLGAMRDMLLSKTERT

Sequences:

>Translated_585_residues
MALAISRGELPVELLSGADMIARFLQDEGVERIYGYPGGAALHIYDALFRQDKVKHILVRHEQAATHAADGYARASGKPG
TVLVTSGPGATNAVTGIATAYMDSIPMVVLCGQVASHLIGEDAFQETDIVGVTRPIVKHSFAIKHPSEIPEVLKKAYYLA
STGRPGPVVVDIPKDMTAPTERYEYVYPKKVKIRSYNPVTRGHSGQIKKAVDMMLKARRPVFYTGGGIVTSGASENLTAL
VRRLGFPITTTLMGIGAYPQTDAQSLSWLGMHGSYEANMAMHHADLIIAIGARFDDRVTNNTSKFCPTAKIVHVDIDPSS
ISKTVRADVPIVGPADSVINEMISLLQGREMANAEALPEWWATIDGWRAEREGKLYEPSQPGERLKPQEVVEAVYHITRG
EAFVTTDVGQHQMFAAQYYKFDKPNRWITSGGLGTMGFGFPAAMGIKQNFPDDIVVCFTGEGSFQMMMQELSTCKQFGTG
VKIINLNNGSLGMVRQWQDLNYKSRHAHSYMESLPDFHKLIEAYGFTALKVERHDQLDAALERMFADNDELVFLDVDVDP
REHVYPMQVPLGAMRDMLLSKTERT
>Mature_584_residues
ALAISRGELPVELLSGADMIARFLQDEGVERIYGYPGGAALHIYDALFRQDKVKHILVRHEQAATHAADGYARASGKPGT
VLVTSGPGATNAVTGIATAYMDSIPMVVLCGQVASHLIGEDAFQETDIVGVTRPIVKHSFAIKHPSEIPEVLKKAYYLAS
TGRPGPVVVDIPKDMTAPTERYEYVYPKKVKIRSYNPVTRGHSGQIKKAVDMMLKARRPVFYTGGGIVTSGASENLTALV
RRLGFPITTTLMGIGAYPQTDAQSLSWLGMHGSYEANMAMHHADLIIAIGARFDDRVTNNTSKFCPTAKIVHVDIDPSSI
SKTVRADVPIVGPADSVINEMISLLQGREMANAEALPEWWATIDGWRAEREGKLYEPSQPGERLKPQEVVEAVYHITRGE
AFVTTDVGQHQMFAAQYYKFDKPNRWITSGGLGTMGFGFPAAMGIKQNFPDDIVVCFTGEGSFQMMMQELSTCKQFGTGV
KIINLNNGSLGMVRQWQDLNYKSRHAHSYMESLPDFHKLIEAYGFTALKVERHDQLDAALERMFADNDELVFLDVDVDPR
EHVYPMQVPLGAMRDMLLSKTERT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=567, Percent_Identity=25.2204585537919, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI21361361, Length=562, Percent_Identity=24.5551601423488, Blast_Score=149, Evalue=8e-36,
Organism=Escherichia coli, GI87081685, Length=578, Percent_Identity=57.0934256055363, Blast_Score=704, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=564, Percent_Identity=38.2978723404255, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1786717, Length=543, Percent_Identity=30.5709023941068, Blast_Score=268, Evalue=7e-73,
Organism=Escherichia coli, GI1787096, Length=564, Percent_Identity=27.4822695035461, Blast_Score=185, Evalue=7e-48,
Organism=Escherichia coli, GI1788716, Length=561, Percent_Identity=23.3511586452763, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=562, Percent_Identity=26.3345195729537, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17531301, Length=562, Percent_Identity=26.3345195729537, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17542570, Length=505, Percent_Identity=25.3465346534653, Blast_Score=121, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323755, Length=571, Percent_Identity=43.0823117338004, Blast_Score=454, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6320816, Length=489, Percent_Identity=23.9263803680982, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321524, Length=500, Percent_Identity=21.6, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI19922626, Length=581, Percent_Identity=23.7521514629948, Blast_Score=146, Evalue=3e-35,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64518; Mature: 64386

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAISRGELPVELLSGADMIARFLQDEGVERIYGYPGGAALHIYDALFRQDKVKHILVR
CEEEECCCCCCHHHHCCHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHH
HEQAATHAADGYARASGKPGTVLVTSGPGATNAVTGIATAYMDSIPMVVLCGQVASHLIG
HHHHHHHHCCCHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
EDAFQETDIVGVTRPIVKHSFAIKHPSEIPEVLKKAYYLASTGRPGPVVVDIPKDMTAPT
CHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCH
ERYEYVYPKKVKIRSYNPVTRGHSGQIKKAVDMMLKARRPVFYTGGGIVTSGASENLTAL
HHHCEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEECCCCCHHHHH
VRRLGFPITTTLMGIGAYPQTDAQSLSWLGMHGSYEANMAMHHADLIIAIGARFDDRVTN
HHHHCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCEEECCEEEEECCCCCCHHCC
NTSKFCPTAKIVHVDIDPSSISKTVRADVPIVGPADSVINEMISLLQGREMANAEALPEW
CCCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHCHHH
WATIDGWRAEREGKLYEPSQPGERLKPQEVVEAVYHITRGEAFVTTDVGQHQMFAAQYYK
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHC
FDKPNRWITSGGLGTMGFGFPAAMGIKQNFPDDIVVCFTGEGSFQMMMQELSTCKQFGTG
CCCCCCEEECCCCCCCCCCCHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCC
VKIINLNNGSLGMVRQWQDLNYKSRHAHSYMESLPDFHKLIEAYGFTALKVERHDQLDAA
EEEEEECCCCCCCEEHHCCCCCHHHHHHHHHHHCHHHHHHHHHHCCEEEEECCHHHHHHH
LERMFADNDELVFLDVDVDPREHVYPMQVPLGAMRDMLLSKTERT
HHHHHCCCCCEEEEEECCCCCCCEEECCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALAISRGELPVELLSGADMIARFLQDEGVERIYGYPGGAALHIYDALFRQDKVKHILVR
EEEECCCCCCHHHHCCHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHH
HEQAATHAADGYARASGKPGTVLVTSGPGATNAVTGIATAYMDSIPMVVLCGQVASHLIG
HHHHHHHHCCCHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
EDAFQETDIVGVTRPIVKHSFAIKHPSEIPEVLKKAYYLASTGRPGPVVVDIPKDMTAPT
CHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCH
ERYEYVYPKKVKIRSYNPVTRGHSGQIKKAVDMMLKARRPVFYTGGGIVTSGASENLTAL
HHHCEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEECCCCCHHHHH
VRRLGFPITTTLMGIGAYPQTDAQSLSWLGMHGSYEANMAMHHADLIIAIGARFDDRVTN
HHHHCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCEEECCEEEEECCCCCCHHCC
NTSKFCPTAKIVHVDIDPSSISKTVRADVPIVGPADSVINEMISLLQGREMANAEALPEW
CCCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHCHHH
WATIDGWRAEREGKLYEPSQPGERLKPQEVVEAVYHITRGEAFVTTDVGQHQMFAAQYYK
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHC
FDKPNRWITSGGLGTMGFGFPAAMGIKQNFPDDIVVCFTGEGSFQMMMQELSTCKQFGTG
CCCCCCEEECCCCCCCCCCCHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCC
VKIINLNNGSLGMVRQWQDLNYKSRHAHSYMESLPDFHKLIEAYGFTALKVERHDQLDAA
EEEEEECCCCCCCEEHHCCCCCHHHHHHHHHHHCHHHHHHHHHHCCEEEEECCHHHHHHH
LERMFADNDELVFLDVDVDPREHVYPMQVPLGAMRDMLLSKTERT
HHHHHCCCCCEEEEEECCCCCCCEEECCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9688822; 12089438 [H]