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The map label for this gene is pcaF [H]

Identifier: 92112435

GI number: 92112435

Start: 339824

End: 341029

Strand: Direct

Name: pcaF [H]

Synonym: Csal_0301

Alternate gene names: 92112435

Gene position: 339824-341029 (Clockwise)

Preceding gene: 92112434

Following gene: 92112436

Centisome position: 9.19

GC content: 68.57

Gene sequence:

>1206_bases
ATGAGCGATGTTTGGTTGTGTCACCCCACGCGTTCCGCCGTGGGACGTTTCGGCGGCTCGCTGGCCAGCGTCCGCCCCGA
CGACCTGGCGGCCACGATTTTCCGGGCCGTGCTGGCCCAGGCGCCGGACCTCGATCCGGCGGCCATCGATGAAGTGATCA
TGGGCTGCGCCAACCAGGCCGGCGAGGACAATCGCAACGTCGCGCGCATGTCGGCGCTGCTGGCCGGGCTGCCGACCAGC
GTGCCCGGCACCACCTTGAATCGCCTGTGCGGGTCGGGCATGGATGCCGTGGGGACGGCATTCCGCGCCATCAAGGCGGG
CGAGTTCGAGCTGGCGCTGGCGGGGGGCGTGGAGTCCATGTCGCGCGCCCCGTTCGTGATGGGCAAGGCCGAGACGGCAT
TCTCGCGCAGCCAGGCGATCGAGGACACGACCATCGGCTGGCGTTTCGTCAACCCGCTGATGAAGAACCACTACGGCGTC
GAGTCGATGCCAGAGACCGCCGAGAACGTGGCCGAGCAGTTCCACGTGTCCCGCGAGGATCAGGATGCCTTCGCCGTGCG
CTCGCAGCATAAGACCGAGCGCGCCCAGCAGTCCGGGCGTCTGGCGCAGGAGATCACGCCGGTCGAGGTGCCGCGTCGCA
AGCAGGAGCCCCTGATCGTCGATCGGGATGAGCATCCGCGCGCAGGCACCACGCTGGAAAAGCTGGCCAAGTTGCCCACG
CCGTTTCGCGATGGCGGCAGCGTGACCGCGGGCAATGCGTCGGGCGTCAACGACGGGGCGGCGGCGATGCTGGTTGCCAG
CGACGCCGCCGTCGCGCGCCATGGTCTCACCCCGATGGCGCGGATCCTGGGCATGGCCACCGCCGGCGTCGAACCCCGCA
TCATGGGCATGGGCCCGGTACCGGCGACGCGCAAGCTGCTCGAGCGTCTGGGTATCGGCATCGATGAGGTCGACATCATC
GAGCTCAACGAAGCCTTTGCCGCCCAGGGGCTGGCATGCCTGCGCGAACTAGGCGTGGCCGATGACGATCCGCGCGTCAA
TCCCAATGGCGGCGCGATCGCGCTGGGGCATCCGCTGGGCATGTCCGGGGCGCGGCTGCTGCTGACCGCGGCGCACGAGC
TGCACGTACAGAACAAGCGTTACGCCCTTTGCACCATGTGTGTCGGCGTGGGCCAGGGCGTGGCCACTTTGATCGAACGC
GTTTGA

Upstream 100 bases:

>100_bases
GACACTACGCCGATGCCTTCCGCGAATGAGCTCGACGTGCTGCGGGATCTCAAGGCGCGTACCGAACGCAAACATGCCGG
CGAGGCATGAGGAGAATTCG

Downstream 100 bases:

>100_bases
GGAGAACGGCATGGCTTTCTTGACGGTGGCGGAGCGCACGATCGCCTATCGCGACAGCGGTGAAGCCGCGCTGCCGGCGG
TGCTGCTGGCACACCCGCTG

Product: Beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSDVWLCHPTRSAVGRFGGSLASVRPDDLAATIFRAVLAQAPDLDPAAIDEVIMGCANQAGEDNRNVARMSALLAGLPTS
VPGTTLNRLCGSGMDAVGTAFRAIKAGEFELALAGGVESMSRAPFVMGKAETAFSRSQAIEDTTIGWRFVNPLMKNHYGV
ESMPETAENVAEQFHVSREDQDAFAVRSQHKTERAQQSGRLAQEITPVEVPRRKQEPLIVDRDEHPRAGTTLEKLAKLPT
PFRDGGSVTAGNASGVNDGAAAMLVASDAAVARHGLTPMARILGMATAGVEPRIMGMGPVPATRKLLERLGIGIDEVDII
ELNEAFAAQGLACLRELGVADDDPRVNPNGGAIALGHPLGMSGARLLLTAAHELHVQNKRYALCTMCVGVGQGVATLIER
V

Sequences:

>Translated_401_residues
MSDVWLCHPTRSAVGRFGGSLASVRPDDLAATIFRAVLAQAPDLDPAAIDEVIMGCANQAGEDNRNVARMSALLAGLPTS
VPGTTLNRLCGSGMDAVGTAFRAIKAGEFELALAGGVESMSRAPFVMGKAETAFSRSQAIEDTTIGWRFVNPLMKNHYGV
ESMPETAENVAEQFHVSREDQDAFAVRSQHKTERAQQSGRLAQEITPVEVPRRKQEPLIVDRDEHPRAGTTLEKLAKLPT
PFRDGGSVTAGNASGVNDGAAAMLVASDAAVARHGLTPMARILGMATAGVEPRIMGMGPVPATRKLLERLGIGIDEVDII
ELNEAFAAQGLACLRELGVADDDPRVNPNGGAIALGHPLGMSGARLLLTAAHELHVQNKRYALCTMCVGVGQGVATLIER
V
>Mature_400_residues
SDVWLCHPTRSAVGRFGGSLASVRPDDLAATIFRAVLAQAPDLDPAAIDEVIMGCANQAGEDNRNVARMSALLAGLPTSV
PGTTLNRLCGSGMDAVGTAFRAIKAGEFELALAGGVESMSRAPFVMGKAETAFSRSQAIEDTTIGWRFVNPLMKNHYGVE
SMPETAENVAEQFHVSREDQDAFAVRSQHKTERAQQSGRLAQEITPVEVPRRKQEPLIVDRDEHPRAGTTLEKLAKLPTP
FRDGGSVTAGNASGVNDGAAAMLVASDAAVARHGLTPMARILGMATAGVEPRIMGMGPVPATRKLLERLGIGIDEVDIIE
LNEAFAAQGLACLRELGVADDDPRVNPNGGAIALGHPLGMSGARLLLTAAHELHVQNKRYALCTMCVGVGQGVATLIERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=398, Percent_Identity=40.7035175879397, Blast_Score=284, Evalue=1e-76,
Organism=Homo sapiens, GI148539872, Length=393, Percent_Identity=41.4758269720102, Blast_Score=280, Evalue=1e-75,
Organism=Homo sapiens, GI4501853, Length=404, Percent_Identity=38.6138613861386, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI4557237, Length=402, Percent_Identity=34.8258706467662, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI4504327, Length=416, Percent_Identity=30.2884615384615, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI194353979, Length=400, Percent_Identity=28.5, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=66.0847880299252, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=44.1687344913151, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI87082165, Length=402, Percent_Identity=43.7810945273632, Blast_Score=292, Evalue=3e-80,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=41.4814814814815, Blast_Score=274, Evalue=7e-75,
Organism=Escherichia coli, GI1788683, Length=387, Percent_Identity=28.9405684754522, Blast_Score=142, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI133906874, Length=400, Percent_Identity=42.25, Blast_Score=295, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI25147385, Length=399, Percent_Identity=35.5889724310777, Blast_Score=232, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI17535921, Length=411, Percent_Identity=33.8199513381995, Blast_Score=207, Evalue=7e-54,
Organism=Caenorhabditis elegans, GI17551802, Length=431, Percent_Identity=29.2343387470998, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17535917, Length=403, Percent_Identity=30.0248138957816, Blast_Score=155, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6322031, Length=412, Percent_Identity=36.6504854368932, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6325229, Length=403, Percent_Identity=34.7394540942928, Blast_Score=212, Evalue=8e-56,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=43.640897755611, Blast_Score=329, Evalue=2e-90,
Organism=Drosophila melanogaster, GI17648125, Length=401, Percent_Identity=40.1496259351621, Blast_Score=278, Evalue=4e-75,
Organism=Drosophila melanogaster, GI24640423, Length=404, Percent_Identity=33.4158415841584, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17137578, Length=412, Percent_Identity=30.5825242718447, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 42325; Mature: 42194

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVWLCHPTRSAVGRFGGSLASVRPDDLAATIFRAVLAQAPDLDPAAIDEVIMGCANQA
CCCCEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
GEDNRNVARMSALLAGLPTSVPGTTLNRLCGSGMDAVGTAFRAIKAGEFELALAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCHHHH
SRAPFVMGKAETAFSRSQAIEDTTIGWRFVNPLMKNHYGVESMPETAENVAEQFHVSRED
HCCCEEECCCHHHHHHHHHCCHHHCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCC
QDAFAVRSQHKTERAQQSGRLAQEITPVEVPRRKQEPLIVDRDEHPRAGTTLEKLAKLPT
CHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHCCC
PFRDGGSVTAGNASGVNDGAAAMLVASDAAVARHGLTPMARILGMATAGVEPRIMGMGPV
CCCCCCCEECCCCCCCCCCCEEEEEECCHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCC
PATRKLLERLGIGIDEVDIIELNEAFAAQGLACLRELGVADDDPRVNPNGGAIALGHPLG
HHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCC
MSGARLLLTAAHELHVQNKRYALCTMCVGVGQGVATLIERV
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure 
SDVWLCHPTRSAVGRFGGSLASVRPDDLAATIFRAVLAQAPDLDPAAIDEVIMGCANQA
CCCEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
GEDNRNVARMSALLAGLPTSVPGTTLNRLCGSGMDAVGTAFRAIKAGEFELALAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCHHHH
SRAPFVMGKAETAFSRSQAIEDTTIGWRFVNPLMKNHYGVESMPETAENVAEQFHVSRED
HCCCEEECCCHHHHHHHHHCCHHHCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCC
QDAFAVRSQHKTERAQQSGRLAQEITPVEVPRRKQEPLIVDRDEHPRAGTTLEKLAKLPT
CHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHCCC
PFRDGGSVTAGNASGVNDGAAAMLVASDAAVARHGLTPMARILGMATAGVEPRIMGMGPV
CCCCCCCEECCCCCCCCCCCEEEEEECCHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCC
PATRKLLERLGIGIDEVDIIELNEAFAAQGLACLRELGVADDDPRVNPNGGAIALGHPLG
HHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCC
MSGARLLLTAAHELHVQNKRYALCTMCVGVGQGVATLIERV
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]