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The map label for this gene is lcfB [H]

Identifier: 92112419

GI number: 92112419

Start: 321729

End: 323564

Strand: Direct

Name: lcfB [H]

Synonym: Csal_0285

Alternate gene names: 92112419

Gene position: 321729-323564 (Clockwise)

Preceding gene: 92112413

Following gene: 92112420

Centisome position: 8.7

GC content: 66.94

Gene sequence:

>1836_bases
ATGTCGTCCCCTTCCGGCAACCGGCCAGACGAGTCACGTTCCGTGAACGATGCGTCCCCGCAGACGCATTGGCTCTTCGG
TGGCGAGGTCTCGGAGTATCCCCGTTGCGTGACCCAGCGCCTCGTGCACTGGGCCGGCGAGACGCCGTCGCGCACGTTCC
TGGCCGAACGCGAGGGGACGCATCGTGCCTGGCGGACGCTGACCTTCGGTGAGGCGATGCCGCGCGTGGCGCGTCTGGCA
CAGGCCCTGCTGGACCATGGCCTGTCGGTCGAGCGGCCGCTGATGGTGCTCTCGGGCAATGGCATCGATCATGCGTTGCT
GGCCTGCGCCGCGATGCACGTGGGCATCCCCTATGTACCGGTATCGCCCTCGTACGCACTGTTGTCGGAGGACTTCGCCA
AGTTGCGTCACGTCGTTGACCTGGTGACGCCGGGGATGATCTTCGTCGACGACGAGGCGCGTTTCGAGGCGGCCCTGGCG
GCGGTTTGCGAGCCCGGCACGACCTGCGTGGCGGTACGTCCGAGCCAGCGGGCACTGGCGCTGGAAACCCTGGCGGCAAC
GCCGGCGAGCGGCGCGGTCGACGACGCCCATGCCGGCGTGACAGCGGATAGCATCGCCAAGCTGCTGTTCACCTCGGGAT
CGACGGGGATGCCAAAGGCGGTGATCAACACGCACCGCATGCTGTGCAGCAACCAGGCCATGCTCGCGCAACGCCTGGCG
TTCTTGTCGCGGCGCAAGCCCGTGCTGGTGGACTGGTTGCCCTGGCACCATACCTTCGGCGGCAACACGATCCTGGGCAT
GACCATCCACAACGGCGGTTCGCTGTACATCGACGACGGCAACCCCACCGCCGAGGGAGTAAGGCGCACGGTGGACAACC
TGCGCGAGATATCGCCGACCTTCTATTGCAACGTGCCCAAGGGGTTCGAGGCCCTGGTCGAGCATCTGCGCGACGATGAC
GCATTGCGCACGTCTTTCTTCCGCGACCTCGAGATGATGCACTTCGGCGGCGCCGTGCTGCCGGTGCATGTACGCGATGC
GCTGGATGCCTTCGGCGAGCGTTCGCTCGGGCATCCCGTGCCCATGCTGACGGGCCTGGGTTCCACCGAGGCGTGCCTGG
CGTTCTGCACCGAGGAGGCCAGCGACACGCCGGGGCTGATCGGTCGGCCCGTGCCGGGCATGTCGCTCAAGCTCGTGGCC
ACGGACGACAAGTGGGAGGCCCGTCTCAAGGGGCTCAATGTCACGCCGGGCTACTGGCGTGACGAGGCGCGCAGCGCCGA
GGCGTTCGACGACGAAGGCTACTATTGCACCGGGGACGCGATGCGTTTCGTCGATCCCGACCGCCCCGAGCGGGGGCTGA
TCTTCGACGGCCGGTTGAGCGAGAACTTCAAGCTCGTGACGGGCACCTGGGTCAATGTCGGCATTCTGCGCTTGCGTCTC
ATCGAGCACTTCGCCCCGCTGGTACAGGACGTGGTGGTGGTCGGCGAGGGGCGTGATGCCCTGGCCGGTTTCGTGGTGCT
GAACGTGCCGGCATGTCGGCAGCGTCTGGGCGATGCGGCACCGTCATCGCTGGACGCATTGGCGCACGACGACACCCTGC
ATCAATGGTTCGCCCAGCGTCTGGCGACGCATCGCCAGGGGCAGTCGAGCTCCATGCGCCTGGAGCGCCTGTTACTGCTG
GATCGGCCGCTGTCCGTCGACCGTGGCGAGCTGACCGACAAGGGCTCTGTCAATCAGCGGCTGGTGCGCGAGCGTTATAC
GGAACTCGCCGAGTGCCTGTATGCCGAGCCGCCTGGGGATGCGCGCGTGATCCTGCCGGATTCGTCGGTGTCGTGA

Upstream 100 bases:

>100_bases
AAAAAAATAGTTACCTATTATAACTATATCCATTGCGTCGTTGATGAAAGAACGAATCGCTCAATAACAAGAGACATCAC
AGACTAATGGAGCCCCTTTC

Downstream 100 bases:

>100_bases
CGTTTCCGCCTTGCCTCCACGCGACCGTTGTGTCACCACTCCCCAATCGAAATGGATGAAACGGTCGAGATGTGACGATG
AAATCCCCTGTACGCATCCT

Product: feruloyl-CoA synthase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 611; Mature: 610

Protein sequence:

>611_residues
MSSPSGNRPDESRSVNDASPQTHWLFGGEVSEYPRCVTQRLVHWAGETPSRTFLAEREGTHRAWRTLTFGEAMPRVARLA
QALLDHGLSVERPLMVLSGNGIDHALLACAAMHVGIPYVPVSPSYALLSEDFAKLRHVVDLVTPGMIFVDDEARFEAALA
AVCEPGTTCVAVRPSQRALALETLAATPASGAVDDAHAGVTADSIAKLLFTSGSTGMPKAVINTHRMLCSNQAMLAQRLA
FLSRRKPVLVDWLPWHHTFGGNTILGMTIHNGGSLYIDDGNPTAEGVRRTVDNLREISPTFYCNVPKGFEALVEHLRDDD
ALRTSFFRDLEMMHFGGAVLPVHVRDALDAFGERSLGHPVPMLTGLGSTEACLAFCTEEASDTPGLIGRPVPGMSLKLVA
TDDKWEARLKGLNVTPGYWRDEARSAEAFDDEGYYCTGDAMRFVDPDRPERGLIFDGRLSENFKLVTGTWVNVGILRLRL
IEHFAPLVQDVVVVGEGRDALAGFVVLNVPACRQRLGDAAPSSLDALAHDDTLHQWFAQRLATHRQGQSSSMRLERLLLL
DRPLSVDRGELTDKGSVNQRLVRERYTELAECLYAEPPGDARVILPDSSVS

Sequences:

>Translated_611_residues
MSSPSGNRPDESRSVNDASPQTHWLFGGEVSEYPRCVTQRLVHWAGETPSRTFLAEREGTHRAWRTLTFGEAMPRVARLA
QALLDHGLSVERPLMVLSGNGIDHALLACAAMHVGIPYVPVSPSYALLSEDFAKLRHVVDLVTPGMIFVDDEARFEAALA
AVCEPGTTCVAVRPSQRALALETLAATPASGAVDDAHAGVTADSIAKLLFTSGSTGMPKAVINTHRMLCSNQAMLAQRLA
FLSRRKPVLVDWLPWHHTFGGNTILGMTIHNGGSLYIDDGNPTAEGVRRTVDNLREISPTFYCNVPKGFEALVEHLRDDD
ALRTSFFRDLEMMHFGGAVLPVHVRDALDAFGERSLGHPVPMLTGLGSTEACLAFCTEEASDTPGLIGRPVPGMSLKLVA
TDDKWEARLKGLNVTPGYWRDEARSAEAFDDEGYYCTGDAMRFVDPDRPERGLIFDGRLSENFKLVTGTWVNVGILRLRL
IEHFAPLVQDVVVVGEGRDALAGFVVLNVPACRQRLGDAAPSSLDALAHDDTLHQWFAQRLATHRQGQSSSMRLERLLLL
DRPLSVDRGELTDKGSVNQRLVRERYTELAECLYAEPPGDARVILPDSSVS
>Mature_610_residues
SSPSGNRPDESRSVNDASPQTHWLFGGEVSEYPRCVTQRLVHWAGETPSRTFLAEREGTHRAWRTLTFGEAMPRVARLAQ
ALLDHGLSVERPLMVLSGNGIDHALLACAAMHVGIPYVPVSPSYALLSEDFAKLRHVVDLVTPGMIFVDDEARFEAALAA
VCEPGTTCVAVRPSQRALALETLAATPASGAVDDAHAGVTADSIAKLLFTSGSTGMPKAVINTHRMLCSNQAMLAQRLAF
LSRRKPVLVDWLPWHHTFGGNTILGMTIHNGGSLYIDDGNPTAEGVRRTVDNLREISPTFYCNVPKGFEALVEHLRDDDA
LRTSFFRDLEMMHFGGAVLPVHVRDALDAFGERSLGHPVPMLTGLGSTEACLAFCTEEASDTPGLIGRPVPGMSLKLVAT
DDKWEARLKGLNVTPGYWRDEARSAEAFDDEGYYCTGDAMRFVDPDRPERGLIFDGRLSENFKLVTGTWVNVGILRLRLI
EHFAPLVQDVVVVGEGRDALAGFVVLNVPACRQRLGDAAPSSLDALAHDDTLHQWFAQRLATHRQGQSSSMRLERLLLLD
RPLSVDRGELTDKGSVNQRLVRERYTELAECLYAEPPGDARVILPDSSVS

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=364, Percent_Identity=25.2747252747253, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI187761343, Length=364, Percent_Identity=25.2747252747253, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17564090, Length=354, Percent_Identity=25.9887005649718, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556552, Length=452, Percent_Identity=23.2300884955752, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17558820, Length=382, Percent_Identity=24.869109947644, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 66817; Mature: 66685

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPSGNRPDESRSVNDASPQTHWLFGGEVSEYPRCVTQRLVHWAGETPSRTFLAEREGT
CCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
HRAWRTLTFGEAMPRVARLAQALLDHGLSVERPLMVLSGNGIDHALLACAAMHVGIPYVP
HHHHHEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEE
VSPSYALLSEDFAKLRHVVDLVTPGMIFVDDEARFEAALAAVCEPGTTCVAVRPSQRALA
CCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCCCCEEEEECCCCHHHH
LETLAATPASGAVDDAHAGVTADSIAKLLFTSGSTGMPKAVINTHRMLCSNQAMLAQRLA
HHHHHCCCCCCCCCCHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHH
FLSRRKPVLVDWLPWHHTFGGNTILGMTIHNGGSLYIDDGNPTAEGVRRTVDNLREISPT
HHHCCCCEEEEECCCEEECCCCEEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCC
FYCNVPKGFEALVEHLRDDDALRTSFFRDLEMMHFGGAVLPVHVRDALDAFGERSLGHPV
EEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHCCCCCCCCC
PMLTGLGSTEACLAFCTEEASDTPGLIGRPVPGMSLKLVATDDKWEARLKGLNVTPGYWR
HHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCCCCC
DEARSAEAFDDEGYYCTGDAMRFVDPDRPERGLIFDGRLSENFKLVTGTWVNVGILRLRL
CCCCCCCCCCCCCCEECCCEEECCCCCCCCCCEEECCCCCCCEEEEEECHHHHHHHHHHH
IEHFAPLVQDVVVVGEGRDALAGFVVLNVPACRQRLGDAAPSSLDALAHDDTLHQWFAQR
HHHHHHHHHHHEEECCCCHHHEEEEEEECHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHH
LATHRQGQSSSMRLERLLLLDRPLSVDRGELTDKGSVNQRLVRERYTELAECLYAEPPGD
HHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
ARVILPDSSVS
CEEEECCCCCC
>Mature Secondary Structure 
SSPSGNRPDESRSVNDASPQTHWLFGGEVSEYPRCVTQRLVHWAGETPSRTFLAEREGT
CCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
HRAWRTLTFGEAMPRVARLAQALLDHGLSVERPLMVLSGNGIDHALLACAAMHVGIPYVP
HHHHHEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEE
VSPSYALLSEDFAKLRHVVDLVTPGMIFVDDEARFEAALAAVCEPGTTCVAVRPSQRALA
CCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCCCCEEEEECCCCHHHH
LETLAATPASGAVDDAHAGVTADSIAKLLFTSGSTGMPKAVINTHRMLCSNQAMLAQRLA
HHHHHCCCCCCCCCCHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHH
FLSRRKPVLVDWLPWHHTFGGNTILGMTIHNGGSLYIDDGNPTAEGVRRTVDNLREISPT
HHHCCCCEEEEECCCEEECCCCEEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCC
FYCNVPKGFEALVEHLRDDDALRTSFFRDLEMMHFGGAVLPVHVRDALDAFGERSLGHPV
EEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHCCCCCCCCC
PMLTGLGSTEACLAFCTEEASDTPGLIGRPVPGMSLKLVATDDKWEARLKGLNVTPGYWR
HHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCCCCC
DEARSAEAFDDEGYYCTGDAMRFVDPDRPERGLIFDGRLSENFKLVTGTWVNVGILRLRL
CCCCCCCCCCCCCCEECCCEEECCCCCCCCCCEEECCCCCCCEEEEEECHHHHHHHHHHH
IEHFAPLVQDVVVVGEGRDALAGFVVLNVPACRQRLGDAAPSSLDALAHDDTLHQWFAQR
HHHHHHHHHHHEEECCCCHHHEEEEEEECHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHH
LATHRQGQSSSMRLERLLLLDRPLSVDRGELTDKGSVNQRLVRERYTELAECLYAEPPGD
HHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
ARVILPDSSVS
CEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]