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The map label for this gene is betT [H]

Identifier: 92112358

GI number: 92112358

Start: 249948

End: 252008

Strand: Direct

Name: betT [H]

Synonym: Csal_0223

Alternate gene names: 92112358

Gene position: 249948-252008 (Clockwise)

Preceding gene: 92112354

Following gene: 92112359

Centisome position: 6.76

GC content: 62.15

Gene sequence:

>2061_bases
ATGACGACACAATCCAATAACGAGGCACCGACACGCGATCGCCTCAATGGGCCGGTGTTCTATAGCTCGGTGATCGGCAT
CGTCGCCTTCTCGCTGTGGACCATGGTCGCTACCGACCAGGCCAATACCATCATCAATGCCATACTCGGCTGGATCTCCA
ACACCTTCGGCTGGTACTACTTCCTGACCGTCGTGATCTACCTGGTCTTCGTGATCTATCTGGGGGTCTCGCGCTACGGC
AGCATCCGACTGGGGCCAGAGCATTCGCGCCCCGACTTCAATATCTTCTCGTGGTCGGCGATGCTGTTCTCCGCGGGGAT
CGGCATCGGCGTGATCTTCTTCGCGCTGGCCGAGCCGTTGACGCAGTTCTACAACGCGCCCGACGCGCCCGAGGATCAGG
TCGCCGCGGCACGCCACGCCATGGAACTGACCTTTCTGCACTGGGGGCTTTCCGGCTGGGGCATCTACACCCTGGTGGGC
ATGGCGCTGGCCTTCTTCAGCTATCGGCACAACCTGCCGCTGACCATCCGCAGCGCGCTCTACCCGATCTTCGGCGAGCG
GATCTACGGCATGATCGGCCACGTGGTCGACACCGCTGCCGTGCTGGGCACGGTATTCGGCATCGCGGCCAGCCTGGGGA
TCGGGGTCATCCAGCTCAACTTCGGCCTGGACTACATGTTCGGCATTCCCAAGGGTACCTGGACCCAGGTGGTGCTGGTG
CTGGGCATCGTGCTGTTCGCGACCATTTCCGCCGTGACCGGGGTGGAACGCGGCATCCGCCGTCTTTCCGAGTTCAATAT
CCTGCTCGCGGTCCTGCTGCTGCTGTTCGTGCTGTTTGCCGGCAAGACGATCTTTTTGCTCAACGCGCTGGTGATGAACA
TCGGCGACTACCTGACCAACTTCGTCAGCCTGTCGTTCAACACCTATGCCTTCGACCGGCCGACCGGCTGGTTGAACGGC
TGGACGCTGTTCTTCTGGGCCTGGTGGATCGCGTGGGGGCCGTTCGTCGGGCTGTTCCTGGCGCGCATCTCGCGGGGCCG
GACGATCCGCACCTTCGTGCTCGGCACCATGACGCTGCCGATCATCTTCATGTTCCTGTGGATGTCGCTGCTGGGTAACA
GTGCCATCGACATGGCGATGAACGGCGCCAGCGAGTTCGGCGAGCAGGTGATGAACAACCCGCCGGCGGGGATCTATCTG
TTCCTCGAATCGTATCCGATGCCGCTTTTGACCACCGCGGCGGTGAGCATCCTGGCGATCGTGTTCTTCATCACCTCGGG
GGATTCCGGGGCGCTGGTTCTCTCGAACTTCACCTCGAAGCTCAAGAACGTCAACAGCGATGCGCCGGTCTGGATGCGTA
TTCTGTGGTCGGCGGTGATCGGCATCCTGACCCTGTCGCTGCTGCTCGCCGGGGGGCTGACCACCTTGCAGAGCGCGGTG
GTGATCACCGGACTGCCATTCTCGATCGTGCTGTTCTTCATGATGGCGGGGCTGCTCAAGGCACTGAAGCTCGAGGCCTT
CAAGGAAGACAGCCGGCGCCTGAGCCTGGCCGGCCAGCTTTCCGGTCGTACCGGTGGCGGCGAGCGCGACTCCCGCAATT
GGCAGCAGCGCCTTCGTCGTGCCATGAGCTTCCCCGGCAAGAAGCAGGCACGGCGCTTCATGGAGGAAACCTGCAAGCCC
GCCATGGAAGCCGTGCGCGACTCGCTGCAGGAGCAAGGCGTGTCCGTCGAGATCAATCAGGGCGTGCAGAACGGCGACGA
CTACCTGTCGCTCAACGTCGATTTCGAGGACGAGCAGAACTTCACCTACCAGGTCTGGAGTCAGGGCTTCTCGACGCCCG
GATTCGCGATGCATGCCCCGCATGCCGACTCGCGCTACTACCGGCTGGAGGTCTACCTGCTCGAGGGTAGCCAGGGTTAC
GACCTGATGGGCTACACCCGCGATCAGGTGATCGGCGACATCCTCGACCAGTACGAACTGCACATGCAGTTCCTGCACCT
CAACCGGATAGAGCCGGGCAACATCAACATGCCCGACAGCCCGGAACAGCCGCCTTCATAA

Upstream 100 bases:

>100_bases
TGCCTCTAAGCTAGTAACTGCGCCGTGACGAGCGCCGTGAAACGCTCGTGTGCCGGCTTCCGTGAACGCAAACCTTGCCT
GAACCACATCAGGAACTCCT

Downstream 100 bases:

>100_bases
GGCGTGCCCGGCCGGCCGCCACCGTCAGGCGGCCGGCCTTCTTGAGAAGCTTGAGCCCCCGGTCGCGATGCGGCCGTGGG
TTTTTGCGTGGGAGGGGCGA

Product: choline transport protein BetT

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 686; Mature: 685

Protein sequence:

>686_residues
MTTQSNNEAPTRDRLNGPVFYSSVIGIVAFSLWTMVATDQANTIINAILGWISNTFGWYYFLTVVIYLVFVIYLGVSRYG
SIRLGPEHSRPDFNIFSWSAMLFSAGIGIGVIFFALAEPLTQFYNAPDAPEDQVAAARHAMELTFLHWGLSGWGIYTLVG
MALAFFSYRHNLPLTIRSALYPIFGERIYGMIGHVVDTAAVLGTVFGIAASLGIGVIQLNFGLDYMFGIPKGTWTQVVLV
LGIVLFATISAVTGVERGIRRLSEFNILLAVLLLLFVLFAGKTIFLLNALVMNIGDYLTNFVSLSFNTYAFDRPTGWLNG
WTLFFWAWWIAWGPFVGLFLARISRGRTIRTFVLGTMTLPIIFMFLWMSLLGNSAIDMAMNGASEFGEQVMNNPPAGIYL
FLESYPMPLLTTAAVSILAIVFFITSGDSGALVLSNFTSKLKNVNSDAPVWMRILWSAVIGILTLSLLLAGGLTTLQSAV
VITGLPFSIVLFFMMAGLLKALKLEAFKEDSRRLSLAGQLSGRTGGGERDSRNWQQRLRRAMSFPGKKQARRFMEETCKP
AMEAVRDSLQEQGVSVEINQGVQNGDDYLSLNVDFEDEQNFTYQVWSQGFSTPGFAMHAPHADSRYYRLEVYLLEGSQGY
DLMGYTRDQVIGDILDQYELHMQFLHLNRIEPGNINMPDSPEQPPS

Sequences:

>Translated_686_residues
MTTQSNNEAPTRDRLNGPVFYSSVIGIVAFSLWTMVATDQANTIINAILGWISNTFGWYYFLTVVIYLVFVIYLGVSRYG
SIRLGPEHSRPDFNIFSWSAMLFSAGIGIGVIFFALAEPLTQFYNAPDAPEDQVAAARHAMELTFLHWGLSGWGIYTLVG
MALAFFSYRHNLPLTIRSALYPIFGERIYGMIGHVVDTAAVLGTVFGIAASLGIGVIQLNFGLDYMFGIPKGTWTQVVLV
LGIVLFATISAVTGVERGIRRLSEFNILLAVLLLLFVLFAGKTIFLLNALVMNIGDYLTNFVSLSFNTYAFDRPTGWLNG
WTLFFWAWWIAWGPFVGLFLARISRGRTIRTFVLGTMTLPIIFMFLWMSLLGNSAIDMAMNGASEFGEQVMNNPPAGIYL
FLESYPMPLLTTAAVSILAIVFFITSGDSGALVLSNFTSKLKNVNSDAPVWMRILWSAVIGILTLSLLLAGGLTTLQSAV
VITGLPFSIVLFFMMAGLLKALKLEAFKEDSRRLSLAGQLSGRTGGGERDSRNWQQRLRRAMSFPGKKQARRFMEETCKP
AMEAVRDSLQEQGVSVEINQGVQNGDDYLSLNVDFEDEQNFTYQVWSQGFSTPGFAMHAPHADSRYYRLEVYLLEGSQGY
DLMGYTRDQVIGDILDQYELHMQFLHLNRIEPGNINMPDSPEQPPS
>Mature_685_residues
TTQSNNEAPTRDRLNGPVFYSSVIGIVAFSLWTMVATDQANTIINAILGWISNTFGWYYFLTVVIYLVFVIYLGVSRYGS
IRLGPEHSRPDFNIFSWSAMLFSAGIGIGVIFFALAEPLTQFYNAPDAPEDQVAAARHAMELTFLHWGLSGWGIYTLVGM
ALAFFSYRHNLPLTIRSALYPIFGERIYGMIGHVVDTAAVLGTVFGIAASLGIGVIQLNFGLDYMFGIPKGTWTQVVLVL
GIVLFATISAVTGVERGIRRLSEFNILLAVLLLLFVLFAGKTIFLLNALVMNIGDYLTNFVSLSFNTYAFDRPTGWLNGW
TLFFWAWWIAWGPFVGLFLARISRGRTIRTFVLGTMTLPIIFMFLWMSLLGNSAIDMAMNGASEFGEQVMNNPPAGIYLF
LESYPMPLLTTAAVSILAIVFFITSGDSGALVLSNFTSKLKNVNSDAPVWMRILWSAVIGILTLSLLLAGGLTTLQSAVV
ITGLPFSIVLFFMMAGLLKALKLEAFKEDSRRLSLAGQLSGRTGGGERDSRNWQQRLRRAMSFPGKKQARRFMEETCKPA
MEAVRDSLQEQGVSVEINQGVQNGDDYLSLNVDFEDEQNFTYQVWSQGFSTPGFAMHAPHADSRYYRLEVYLLEGSQGYD
LMGYTRDQVIGDILDQYELHMQFLHLNRIEPGNINMPDSPEQPPS

Specific function: High-affinity uptake of choline driven by a proton- motive force [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=680, Percent_Identity=51.7647058823529, Blast_Score=727, Evalue=0.0,
Organism=Escherichia coli, GI1788102, Length=450, Percent_Identity=30.2222222222222, Blast_Score=185, Evalue=8e-48,
Organism=Escherichia coli, GI1786224, Length=500, Percent_Identity=27, Blast_Score=169, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 76313; Mature: 76182

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQSNNEAPTRDRLNGPVFYSSVIGIVAFSLWTMVATDQANTIINAILGWISNTFGWYY
CCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FLTVVIYLVFVIYLGVSRYGSIRLGPEHSRPDFNIFSWSAMLFSAGIGIGVIFFALAEPL
HHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
TQFYNAPDAPEDQVAAARHAMELTFLHWGLSGWGIYTLVGMALAFFSYRHNLPLTIRSAL
HHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHH
YPIFGERIYGMIGHVVDTAAVLGTVFGIAASLGIGVIQLNFGLDYMFGIPKGTWTQVVLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEECCCCHHHHHHHH
LGIVLFATISAVTGVERGIRRLSEFNILLAVLLLLFVLFAGKTIFLLNALVMNIGDYLTN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FVSLSFNTYAFDRPTGWLNGWTLFFWAWWIAWGPFVGLFLARISRGRTIRTFVLGTMTLP
HHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHH
IIFMFLWMSLLGNSAIDMAMNGASEFGEQVMNNPPAGIYLFLESYPMPLLTTAAVSILAI
HHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHH
VFFITSGDSGALVLSNFTSKLKNVNSDAPVWMRILWSAVIGILTLSLLLAGGLTTLQSAV
HHHHCCCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VITGLPFSIVLFFMMAGLLKALKLEAFKEDSRRLSLAGQLSGRTGGGERDSRNWQQRLRR
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHCCCCCCCCCCCCCHHHHHHHHH
AMSFPGKKQARRFMEETCKPAMEAVRDSLQEQGVSVEINQGVQNGDDYLSLNVDFEDEQN
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCCCCC
FTYQVWSQGFSTPGFAMHAPHADSRYYRLEVYLLEGSQGYDLMGYTRDQVIGDILDQYEL
CHHHHHHCCCCCCCCEEECCCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
HMQFLHLNRIEPGNINMPDSPEQPPS
HHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTQSNNEAPTRDRLNGPVFYSSVIGIVAFSLWTMVATDQANTIINAILGWISNTFGWYY
CCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FLTVVIYLVFVIYLGVSRYGSIRLGPEHSRPDFNIFSWSAMLFSAGIGIGVIFFALAEPL
HHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
TQFYNAPDAPEDQVAAARHAMELTFLHWGLSGWGIYTLVGMALAFFSYRHNLPLTIRSAL
HHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHH
YPIFGERIYGMIGHVVDTAAVLGTVFGIAASLGIGVIQLNFGLDYMFGIPKGTWTQVVLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEECCCCHHHHHHHH
LGIVLFATISAVTGVERGIRRLSEFNILLAVLLLLFVLFAGKTIFLLNALVMNIGDYLTN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FVSLSFNTYAFDRPTGWLNGWTLFFWAWWIAWGPFVGLFLARISRGRTIRTFVLGTMTLP
HHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHH
IIFMFLWMSLLGNSAIDMAMNGASEFGEQVMNNPPAGIYLFLESYPMPLLTTAAVSILAI
HHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHH
VFFITSGDSGALVLSNFTSKLKNVNSDAPVWMRILWSAVIGILTLSLLLAGGLTTLQSAV
HHHHCCCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VITGLPFSIVLFFMMAGLLKALKLEAFKEDSRRLSLAGQLSGRTGGGERDSRNWQQRLRR
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHCCCCCCCCCCCCCHHHHHHHHH
AMSFPGKKQARRFMEETCKPAMEAVRDSLQEQGVSVEINQGVQNGDDYLSLNVDFEDEQN
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCCCCC
FTYQVWSQGFSTPGFAMHAPHADSRYYRLEVYLLEGSQGYDLMGYTRDQVIGDILDQYEL
CHHHHHHCCCCCCCCEEECCCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
HMQFLHLNRIEPGNINMPDSPEQPPS
HHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]