Click here to switch to the map view.

The map label for this gene is ilvA [H]

Identifier: 92112156

GI number: 92112156

Start: 24625

End: 26139

Strand: Direct

Name: ilvA [H]

Synonym: Csal_0020

Alternate gene names: 92112156

Gene position: 24625-26139 (Clockwise)

Preceding gene: 92112154

Following gene: 92112159

Centisome position: 0.67

GC content: 63.83

Gene sequence:

>1515_bases
ATGCTAGAAGAGACCGTCAAGAAAATCCTCCAGGCTCGCGTTTACGAAGCGGCCCGGGAAACGCCGATATCCCCTGCTCC
CTTTCTCTCCCGCCGTCTCAACAACACGATTCTGATCAAGCGCGAGGATTTGCAGCCGGTCTATTCCTTCAAGATTCGCG
GCGCCTACAACAAGATGGCCCAGCTGAGCGATGAACAGAAGGCCAAGGGCGTGATCGCCGCGTCCGCCGGCAACCATGCC
CAGGGCCTGGCCATGGCCGCCAAGCAGATGGGCGTCAAGGCGGTCATCGTGATGCCGCGCATCACGCCCGACATCAAGGT
CCAGGCCGTGCGCGCGCGTGGCGCCAAGGTCGTGCTCAAGGGCGATGCCTTCGGCGAGGCGCTGGCACATGCGCGCGAGC
TGATCGACGAGCATGGCTACACCTACATTCCGCCCTTCGACGATAACGACGTGATCGCCGGCCAGGGCACGGTGGGCATG
GAGATCCTGCGTCAGCACAGCGGACCGCTGGACGCGGTATTCGTGCCCGTGGGCGGTGGCGGCCTGCTCGCCGGCGTGGT
GGCGTACATCAAGTACCTGCGCCCCGAGATCAAGGTGTACGGGGTCGAGGCGGAAGACGCTGCCTGCCTCAAGGCGGCCC
TGGAAGCCGGCGAACGGGTCACCCTCGACCAGGTCGGCGTGTTCGCCGAGGGCGTCGCCGTGGCGCAGATCGGCGAAGCG
CCGTTCGAGATCCTGCGCCACTGGGTGGATGGCGTGATCACCGTCACCACCGATGAGATGTGCGCGGCGGTCAAGGACAT
CTTCGAGGATACGCGGGCGGTCGCCGAGACCTCCGGCGCGCTGTCGCTGGCGGGGCTCAAGAAATACATCCAGCAGCAGA
ACGCCGAGGGCGAGACCCTGCTGTGCATCAACTCGGGCGCCAACACCAATTTCGATCGTCTGCAGCACATCGCCGAGCGC
ACGGAGCTGGGCGAGCAGCGCGAGGCGATTCTGGCGGTGACGATTCCCGAACGGCCGGGCAGTTTCAAGAAATTCTGCAA
GACCATCGGCAAGCGCATGGTCACCGAGTTCAATTACCGCTATGCCGACCCCGACCACGCGCACATCTTCGTCGGCGTGC
AGGTCAAGCCGGGCGGCGAGGACCGCCAGGCGGTGATCGACAAGTTGCGCGAGGCCGGTTATCCGGTGGAGGACCTCACC
GACAACGAACTGGCCAAGCTGCATATTCGCCATCTCGGTGGCGGGCGTCCCAAGGAGCACTTCAGCGAAGAAGTCTACCG
GTTCGAGTTCCCCGAACGCCCCGGGGCGCTGATGAACTTCCTGACTCATCTGCCCGGCGACTGGAACATTTCACTGTTCC
ACTACCGCAACCATGGCGCGGCGTATGGCCGAGTGCTGGTGGGCATGCAGATCCCCAATGGCGCCCGGGCGCATGTCGAG
GAACATTTCGAACGCATCGGCTATCGCTACTGGAAGGAATCCGACAATCCCGCCTATCGTCTGTTCATGGCCTGA

Upstream 100 bases:

>100_bases
CGTGCCGCTCGCGGCGACGGCGTCTTGCCCGATTCGGCGGGGCGCGGGGAGTGGGAGCCGGTTTTCCAATGAGACGCGCG
AATTCGCAGGACACACCAGA

Downstream 100 bases:

>100_bases
GACGGGGACTTCGAGACGACGCTGCCTTCATAGCCGCCGATTATGAGAAACTGTCTCCCGATAGGCTCTCATGTCGGCGG
CATGAAGGAAGTCCGGCGCG

Product: L-threonine ammonia-lyase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MLEETVKKILQARVYEAARETPISPAPFLSRRLNNTILIKREDLQPVYSFKIRGAYNKMAQLSDEQKAKGVIAASAGNHA
QGLAMAAKQMGVKAVIVMPRITPDIKVQAVRARGAKVVLKGDAFGEALAHARELIDEHGYTYIPPFDDNDVIAGQGTVGM
EILRQHSGPLDAVFVPVGGGGLLAGVVAYIKYLRPEIKVYGVEAEDAACLKAALEAGERVTLDQVGVFAEGVAVAQIGEA
PFEILRHWVDGVITVTTDEMCAAVKDIFEDTRAVAETSGALSLAGLKKYIQQQNAEGETLLCINSGANTNFDRLQHIAER
TELGEQREAILAVTIPERPGSFKKFCKTIGKRMVTEFNYRYADPDHAHIFVGVQVKPGGEDRQAVIDKLREAGYPVEDLT
DNELAKLHIRHLGGGRPKEHFSEEVYRFEFPERPGALMNFLTHLPGDWNISLFHYRNHGAAYGRVLVGMQIPNGARAHVE
EHFERIGYRYWKESDNPAYRLFMA

Sequences:

>Translated_504_residues
MLEETVKKILQARVYEAARETPISPAPFLSRRLNNTILIKREDLQPVYSFKIRGAYNKMAQLSDEQKAKGVIAASAGNHA
QGLAMAAKQMGVKAVIVMPRITPDIKVQAVRARGAKVVLKGDAFGEALAHARELIDEHGYTYIPPFDDNDVIAGQGTVGM
EILRQHSGPLDAVFVPVGGGGLLAGVVAYIKYLRPEIKVYGVEAEDAACLKAALEAGERVTLDQVGVFAEGVAVAQIGEA
PFEILRHWVDGVITVTTDEMCAAVKDIFEDTRAVAETSGALSLAGLKKYIQQQNAEGETLLCINSGANTNFDRLQHIAER
TELGEQREAILAVTIPERPGSFKKFCKTIGKRMVTEFNYRYADPDHAHIFVGVQVKPGGEDRQAVIDKLREAGYPVEDLT
DNELAKLHIRHLGGGRPKEHFSEEVYRFEFPERPGALMNFLTHLPGDWNISLFHYRNHGAAYGRVLVGMQIPNGARAHVE
EHFERIGYRYWKESDNPAYRLFMA
>Mature_504_residues
MLEETVKKILQARVYEAARETPISPAPFLSRRLNNTILIKREDLQPVYSFKIRGAYNKMAQLSDEQKAKGVIAASAGNHA
QGLAMAAKQMGVKAVIVMPRITPDIKVQAVRARGAKVVLKGDAFGEALAHARELIDEHGYTYIPPFDDNDVIAGQGTVGM
EILRQHSGPLDAVFVPVGGGGLLAGVVAYIKYLRPEIKVYGVEAEDAACLKAALEAGERVTLDQVGVFAEGVAVAQIGEA
PFEILRHWVDGVITVTTDEMCAAVKDIFEDTRAVAETSGALSLAGLKKYIQQQNAEGETLLCINSGANTNFDRLQHIAER
TELGEQREAILAVTIPERPGSFKKFCKTIGKRMVTEFNYRYADPDHAHIFVGVQVKPGGEDRQAVIDKLREAGYPVEDLT
DNELAKLHIRHLGGGRPKEHFSEEVYRFEFPERPGALMNFLTHLPGDWNISLFHYRNHGAAYGRVLVGMQIPNGARAHVE
EHFERIGYRYWKESDNPAYRLFMA

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=330, Percent_Identity=33.030303030303, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI19923959, Length=316, Percent_Identity=27.8481012658228, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI33469958, Length=313, Percent_Identity=25.2396166134185, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1790207, Length=501, Percent_Identity=56.8862275449102, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI1789505, Length=312, Percent_Identity=37.8205128205128, Blast_Score=191, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI71991565, Length=372, Percent_Identity=34.4086021505376, Blast_Score=178, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17537387, Length=343, Percent_Identity=34.1107871720117, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17508781, Length=280, Percent_Identity=36.0714285714286, Blast_Score=144, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320930, Length=512, Percent_Identity=50, Blast_Score=464, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6322631, Length=326, Percent_Identity=36.8098159509202, Blast_Score=180, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6319788, Length=240, Percent_Identity=26.6666666666667, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21355833, Length=310, Percent_Identity=31.9354838709677, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24645328, Length=249, Percent_Identity=30.9236947791165, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 55702; Mature: 55702

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEETVKKILQARVYEAARETPISPAPFLSRRLNNTILIKREDLQPVYSFKIRGAYNKMA
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEECCHHHHHH
QLSDEQKAKGVIAASAGNHAQGLAMAAKQMGVKAVIVMPRITPDIKVQAVRARGAKVVLK
HCCCHHHHCEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCCEEEEE
GDAFGEALAHARELIDEHGYTYIPPFDDNDVIAGQGTVGMEILRQHSGPLDAVFVPVGGG
CCHHHHHHHHHHHHHHHCCCEECCCCCCCCEEECCCCHHHHHHHHCCCCCCEEEEEECCC
GLLAGVVAYIKYLRPEIKVYGVEAEDAACLKAALEAGERVTLDQVGVFAEGVAVAQIGEA
HHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCEEEHHHHHHHHCCCHHHHHCCC
PFEILRHWVDGVITVTTDEMCAAVKDIFEDTRAVAETSGALSLAGLKKYIQQQNAEGETL
HHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEE
LCINSGANTNFDRLQHIAERTELGEQREAILAVTIPERPGSFKKFCKTIGKRMVTEFNYR
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCE
YADPDHAHIFVGVQVKPGGEDRQAVIDKLREAGYPVEDLTDNELAKLHIRHLGGGRPKEH
ECCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHH
FSEEVYRFEFPERPGALMNFLTHLPGDWNISLFHYRNHGAAYGRVLVGMQIPNGARAHVE
HHHHHHEEECCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCHHHHH
EHFERIGYRYWKESDNPAYRLFMA
HHHHHHCHHHHCCCCCCEEEEEEC
>Mature Secondary Structure
MLEETVKKILQARVYEAARETPISPAPFLSRRLNNTILIKREDLQPVYSFKIRGAYNKMA
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEECCHHHHHH
QLSDEQKAKGVIAASAGNHAQGLAMAAKQMGVKAVIVMPRITPDIKVQAVRARGAKVVLK
HCCCHHHHCEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCCEEEEE
GDAFGEALAHARELIDEHGYTYIPPFDDNDVIAGQGTVGMEILRQHSGPLDAVFVPVGGG
CCHHHHHHHHHHHHHHHCCCEECCCCCCCCEEECCCCHHHHHHHHCCCCCCEEEEEECCC
GLLAGVVAYIKYLRPEIKVYGVEAEDAACLKAALEAGERVTLDQVGVFAEGVAVAQIGEA
HHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCEEEHHHHHHHHCCCHHHHHCCC
PFEILRHWVDGVITVTTDEMCAAVKDIFEDTRAVAETSGALSLAGLKKYIQQQNAEGETL
HHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEE
LCINSGANTNFDRLQHIAERTELGEQREAILAVTIPERPGSFKKFCKTIGKRMVTEFNYR
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCE
YADPDHAHIFVGVQVKPGGEDRQAVIDKLREAGYPVEDLTDNELAKLHIRHLGGGRPKEH
ECCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHH
FSEEVYRFEFPERPGALMNFLTHLPGDWNISLFHYRNHGAAYGRVLVGMQIPNGARAHVE
HHHHHHEEECCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCHHHHH
EHFERIGYRYWKESDNPAYRLFMA
HHHHHHCHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA