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The map label for this gene is gyrB [H]

Identifier: 92112140

GI number: 92112140

Start: 3937

End: 6354

Strand: Direct

Name: gyrB [H]

Synonym: Csal_0004

Alternate gene names: 92112140

Gene position: 3937-6354 (Clockwise)

Preceding gene: 92112139

Following gene: 92112144

Centisome position: 0.11

GC content: 61.21

Gene sequence:

>2418_bases
ATGAGCGAACAAGCTTACGACTCATCGAGCATCAAGGTCCTCAAGGGACTGGATGCGGTACGCAAGCGCCCCGGCATGTA
CATCGGCGATACCGATGACGGCACGGGCCTGCATCACATGGTCTTCGAACTGGTGGACAACTCCATCGACGAAGCGCTCG
CGGGGCATTGCAGCGAAATTCGCGTGGTGATCCATCCCGATGAGTCCATCACCGTCAGTGACAACGGGCGCGGTATCCCC
ACCGATATTCACTCGGAAGAGGGCGTTTCGGCAGCAGAGGTCATCATGACGGTGCTGCACGCCGGCGGCAAGTTCGACGA
CAACTCCTACAAGGTCTCCGGTGGGCTGCACGGGGTCGGCGTCTCCGTCGTCAACGCGCTCTCCGAGGAGCTCAAGCTGA
CCGTCTGGCGGGCCGGACAGGTCTACGAGCAGATCTACCATCACGGCGTTCCCGATTCACCGTTGGACGTGGTGGGCAAG
ACCGAGAAGAGTGGCACGCAGGTCAACTTCAAGCCCTCGACCGAGACGTTCGCCAACATCGAGTTTCACTACGACATCCT
GGCCAAGCGTCTGCGCGAGCTGTCGTTCCTCAATTCCGGTGTGGCGATCCGCTTGATCGACGAGCGTTCCGGCGCCGAGG
AGCTGTTCCACTACGAGGGCGGCCTGCGGGCGTTCGTCGATCACCTCAACACCAACAAGACGGCGTTGAACCAGATCTTC
CACTTCGAGACCCAGCGTGACGACGGGATTGCCGTCGAGGTCGCGATGCAGTGGAACGACACGTTCTCCGAGAACATCTT
CTGCTACACCAACAACATTCCCCAGCACGATGGCGGCACCCACCTGGCCGGCTTCCGGGCTGCGCTGACGCGGACGCTCA
ATCACTACATCGAAAGCGAAGGTTTGTTGAAGAAGGCGAAGGTGAACACCTCCGGCGATGATGCGCGCGAAGGTCTGACG
GCCATCGTCTCGGTGAAGGTGCCGGACCCGAAGTTTTCTTCGCAGACCAAGGACAAGCTGGTCTCCTCCGAGGTCAAGAC
GGCGGTCGAGCAGGAGATGGGGCGCCAGTTCTCGGACTACCTCGTCGAGCGGCCCAACGAAGCCAAGGCCATCGTCAACA
AGATGCTCGATGCGGCGCGGGCACGCGAAGCGGCACGCAAGGCACGCGACATGACGCGTCGCAAGGGGGCTCTGGATATC
GCCGGGTTGCCCGGCAAGCTGGCCGACTGCCAGGAAAAGGATCCCAGCCTTTCCGAACTCTATCTGGTCGAGGGCGACTC
GGCAGGCGGCAGTGCCAAGCAGGGCCGCGACCGTCGTACCCAGGCGATCCTGCCGCTCAAGGGCAAGATCCTGAACGTCG
AGAAGGCGCGCTTCGACAAGATGCTTTCCTCGGCGGAGGTCGGCACGCTGATCACGGCGCTTGGCTGCGGGATCGGGCGC
GAGGAGTTCAATCCCGACAAGCTGCGCTATCATTCGATCATCATCATGACCGACGCCGACGTCGACGGCTCGCACATCCG
CACGCTGCTGCTGACGTTCTTCTTCCGTCAGATGCCCGAGCTGATCGAGCGGGGACATGTCTTCATCGCCCAGCCGCCGC
TTTACAAGATCAAGCGTGGCAAGCAGGAGACGTACCTCAAGGACGAACAGGCGCTGACCGACTATCTGACGACCACGGCT
CTGGATGGCGCTCGCCTTTACGTGAACGCCAACGCGCCGGGCATCGCCGGTGAGCAGCTGGAAAACCTGGTCAGCCAGTA
TCGCGATGTCATGCGGCGCATCGACCGGCTCTCGCGCGTCTATCCCAACCTGGTGCTGCGCAAGATCGTGCATACCGCCA
AGCTTGAGAATGAAAGCCTCAAGGACCGCGTGACCATGCAGAACTGGATCGACCATCTGCAGGCGGAGATGGACAGCATC
ATCGCCTACGAGGGTGGCCCGCGTTACACCTTCCAGTTGCAGGAAGACACCGAACGAGGCTTGTTCCTGCCCAGCGTAGC
CTTGACCGCACATGGCGTGACCACCGATTACGTCTGGGGCGTCGACTTCTTCCGCAGTGCCGATTATCACGCCATGACGG
CACTCGGCGAGACGCTGGATGGCTTGCTCGAGGAAAGTGCCTATGTCGCGCGTGGCGAGCGTCAGCGCTCGGTGGGCACG
TTCTACGAAGCCCTCGACTGGCTGATGAGTGAAGCCCAGCGTGGCCTGAGCGTACAGCGCTACAAGGGACTCGGCGAGAT
GAACCCCGAGCAGTTGTGGGAAACCACCATGGATCCCCAGTCTCGGCGGATGCTGCGTGTATCCATCGAGGATGCCGTGG
CGTCTGACATGATGTTCAATACCCTGATGGGCGATGAAGTCGAACCGCGTCGTGACTTCATCGAACGCAATGCCCTGGTC
GCCAATCTGGATATCTGA

Upstream 100 bases:

>100_bases
GAGGCGCTCGCGAATGCATGGCAGTCGGAAACGGATGTCGCGATGTTTCACGTGGAACACGGGCGACTGCTCACGGAATG
ACTTCACGACGGAGTAGGCA

Downstream 100 bases:

>100_bases
TGTTTCACGTGAAACATCGTCGCTAGGCTGAGTCCAGTGCGGCGAGTAACAGCGGTATGAGAAGAGGCCGGCAACGTCAC
GTTGCCGGCCTTTTTTGTAT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 805; Mature: 804

Protein sequence:

>805_residues
MSEQAYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFELVDNSIDEALAGHCSEIRVVIHPDESITVSDNGRGIP
TDIHSEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEELKLTVWRAGQVYEQIYHHGVPDSPLDVVGK
TEKSGTQVNFKPSTETFANIEFHYDILAKRLRELSFLNSGVAIRLIDERSGAEELFHYEGGLRAFVDHLNTNKTALNQIF
HFETQRDDGIAVEVAMQWNDTFSENIFCYTNNIPQHDGGTHLAGFRAALTRTLNHYIESEGLLKKAKVNTSGDDAREGLT
AIVSVKVPDPKFSSQTKDKLVSSEVKTAVEQEMGRQFSDYLVERPNEAKAIVNKMLDAARAREAARKARDMTRRKGALDI
AGLPGKLADCQEKDPSLSELYLVEGDSAGGSAKQGRDRRTQAILPLKGKILNVEKARFDKMLSSAEVGTLITALGCGIGR
EEFNPDKLRYHSIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGHVFIAQPPLYKIKRGKQETYLKDEQALTDYLTTTA
LDGARLYVNANAPGIAGEQLENLVSQYRDVMRRIDRLSRVYPNLVLRKIVHTAKLENESLKDRVTMQNWIDHLQAEMDSI
IAYEGGPRYTFQLQEDTERGLFLPSVALTAHGVTTDYVWGVDFFRSADYHAMTALGETLDGLLEESAYVARGERQRSVGT
FYEALDWLMSEAQRGLSVQRYKGLGEMNPEQLWETTMDPQSRRMLRVSIEDAVASDMMFNTLMGDEVEPRRDFIERNALV
ANLDI

Sequences:

>Translated_805_residues
MSEQAYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFELVDNSIDEALAGHCSEIRVVIHPDESITVSDNGRGIP
TDIHSEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEELKLTVWRAGQVYEQIYHHGVPDSPLDVVGK
TEKSGTQVNFKPSTETFANIEFHYDILAKRLRELSFLNSGVAIRLIDERSGAEELFHYEGGLRAFVDHLNTNKTALNQIF
HFETQRDDGIAVEVAMQWNDTFSENIFCYTNNIPQHDGGTHLAGFRAALTRTLNHYIESEGLLKKAKVNTSGDDAREGLT
AIVSVKVPDPKFSSQTKDKLVSSEVKTAVEQEMGRQFSDYLVERPNEAKAIVNKMLDAARAREAARKARDMTRRKGALDI
AGLPGKLADCQEKDPSLSELYLVEGDSAGGSAKQGRDRRTQAILPLKGKILNVEKARFDKMLSSAEVGTLITALGCGIGR
EEFNPDKLRYHSIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGHVFIAQPPLYKIKRGKQETYLKDEQALTDYLTTTA
LDGARLYVNANAPGIAGEQLENLVSQYRDVMRRIDRLSRVYPNLVLRKIVHTAKLENESLKDRVTMQNWIDHLQAEMDSI
IAYEGGPRYTFQLQEDTERGLFLPSVALTAHGVTTDYVWGVDFFRSADYHAMTALGETLDGLLEESAYVARGERQRSVGT
FYEALDWLMSEAQRGLSVQRYKGLGEMNPEQLWETTMDPQSRRMLRVSIEDAVASDMMFNTLMGDEVEPRRDFIERNALV
ANLDI
>Mature_804_residues
SEQAYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFELVDNSIDEALAGHCSEIRVVIHPDESITVSDNGRGIPT
DIHSEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEELKLTVWRAGQVYEQIYHHGVPDSPLDVVGKT
EKSGTQVNFKPSTETFANIEFHYDILAKRLRELSFLNSGVAIRLIDERSGAEELFHYEGGLRAFVDHLNTNKTALNQIFH
FETQRDDGIAVEVAMQWNDTFSENIFCYTNNIPQHDGGTHLAGFRAALTRTLNHYIESEGLLKKAKVNTSGDDAREGLTA
IVSVKVPDPKFSSQTKDKLVSSEVKTAVEQEMGRQFSDYLVERPNEAKAIVNKMLDAARAREAARKARDMTRRKGALDIA
GLPGKLADCQEKDPSLSELYLVEGDSAGGSAKQGRDRRTQAILPLKGKILNVEKARFDKMLSSAEVGTLITALGCGIGRE
EFNPDKLRYHSIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGHVFIAQPPLYKIKRGKQETYLKDEQALTDYLTTTAL
DGARLYVNANAPGIAGEQLENLVSQYRDVMRRIDRLSRVYPNLVLRKIVHTAKLENESLKDRVTMQNWIDHLQAEMDSII
AYEGGPRYTFQLQEDTERGLFLPSVALTAHGVTTDYVWGVDFFRSADYHAMTALGETLDGLLEESAYVARGERQRSVGTF
YEALDWLMSEAQRGLSVQRYKGLGEMNPEQLWETTMDPQSRRMLRVSIEDAVASDMMFNTLMGDEVEPRRDFIERNALVA
NLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=560, Percent_Identity=26.0714285714286, Blast_Score=137, Evalue=5e-32,
Organism=Homo sapiens, GI19913406, Length=559, Percent_Identity=25.7602862254025, Blast_Score=134, Evalue=5e-31,
Organism=Escherichia coli, GI48994957, Length=804, Percent_Identity=69.0298507462687, Blast_Score=1142, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=550, Percent_Identity=40.3636363636364, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17535065, Length=535, Percent_Identity=26.1682242990654, Blast_Score=137, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI212645845, Length=497, Percent_Identity=24.1448692152917, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=155, Percent_Identity=34.8387096774194, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=587, Percent_Identity=25.3833049403748, Blast_Score=134, Evalue=5e-32,
Organism=Drosophila melanogaster, GI17136538, Length=517, Percent_Identity=24.9516441005803, Blast_Score=135, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 89882; Mature: 89750

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQAYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFELVDNSIDEALAGHCSEI
CCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
RVVIHPDESITVSDNGRGIPTDIHSEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVG
EEEECCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHH
VSVVNALSEELKLTVWRAGQVYEQIYHHGVPDSPLDVVGKTEKSGTQVNFKPSTETFANI
HHHHHHHHHHHEEEEEHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCEEEECCCCCCEEEE
EFHYDILAKRLRELSFLNSGVAIRLIDERSGAEELFHYEGGLRAFVDHLNTNKTALNQIF
EEHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCHHHHHHHHCCCHHHHHHHH
HFETQRDDGIAVEVAMQWNDTFSENIFCYTNNIPQHDGGTHLAGFRAALTRTLNHYIESE
HHCCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GLLKKAKVNTSGDDAREGLTAIVSVKVPDPKFSSQTKDKLVSSEVKTAVEQEMGRQFSDY
CCHHHHCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVERPNEAKAIVNKMLDAARAREAARKARDMTRRKGALDIAGLPGKLADCQEKDPSLSEL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCEE
YLVEGDSAGGSAKQGRDRRTQAILPLKGKILNVEKARFDKMLSSAEVGTLITALGCGIGR
EEEECCCCCCCCCCCCCCHHCEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EEFNPDKLRYHSIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGHVFIAQPPLYKIKRG
CCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHCC
KQETYLKDEQALTDYLTTTALDGARLYVNANAPGIAGEQLENLVSQYRDVMRRIDRLSRV
CHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YPNLVLRKIVHTAKLENESLKDRVTMQNWIDHLQAEMDSIIAYEGGPRYTFQLQEDTERG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEECCCCCCC
LFLPSVALTAHGVTTDYVWGVDFFRSADYHAMTALGETLDGLLEESAYVARGERQRSVGT
EECCCHHHHCCCCCCCEEECHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FYEALDWLMSEAQRGLSVQRYKGLGEMNPEQLWETTMDPQSRRMLRVSIEDAVASDMMFN
HHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHH
TLMGDEVEPRRDFIERNALVANLDI
HHCCCCCCHHHHHHHHCCEEEECCC
>Mature Secondary Structure 
SEQAYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFELVDNSIDEALAGHCSEI
CCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
RVVIHPDESITVSDNGRGIPTDIHSEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVG
EEEECCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHH
VSVVNALSEELKLTVWRAGQVYEQIYHHGVPDSPLDVVGKTEKSGTQVNFKPSTETFANI
HHHHHHHHHHHEEEEEHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCEEEECCCCCCEEEE
EFHYDILAKRLRELSFLNSGVAIRLIDERSGAEELFHYEGGLRAFVDHLNTNKTALNQIF
EEHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCHHHHHHHHCCCHHHHHHHH
HFETQRDDGIAVEVAMQWNDTFSENIFCYTNNIPQHDGGTHLAGFRAALTRTLNHYIESE
HHCCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GLLKKAKVNTSGDDAREGLTAIVSVKVPDPKFSSQTKDKLVSSEVKTAVEQEMGRQFSDY
CCHHHHCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVERPNEAKAIVNKMLDAARAREAARKARDMTRRKGALDIAGLPGKLADCQEKDPSLSEL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCEE
YLVEGDSAGGSAKQGRDRRTQAILPLKGKILNVEKARFDKMLSSAEVGTLITALGCGIGR
EEEECCCCCCCCCCCCCCHHCEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EEFNPDKLRYHSIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGHVFIAQPPLYKIKRG
CCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHCC
KQETYLKDEQALTDYLTTTALDGARLYVNANAPGIAGEQLENLVSQYRDVMRRIDRLSRV
CHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YPNLVLRKIVHTAKLENESLKDRVTMQNWIDHLQAEMDSIIAYEGGPRYTFQLQEDTERG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEECCCCCCC
LFLPSVALTAHGVTTDYVWGVDFFRSADYHAMTALGETLDGLLEESAYVARGERQRSVGT
EECCCHHHHCCCCCCCEEECHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FYEALDWLMSEAQRGLSVQRYKGLGEMNPEQLWETTMDPQSRRMLRVSIEDAVASDMMFN
HHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHH
TLMGDEVEPRRDFIERNALVANLDI
HHCCCCCCHHHHHHHHCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]