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The map label for this gene is ptsN [H]

Identifier: 91790441

GI number: 91790441

Start: 4891933

End: 4892406

Strand: Reverse

Name: ptsN [H]

Synonym: Bpro_4612

Alternate gene names: 91790441

Gene position: 4892406-4891933 (Counterclockwise)

Preceding gene: 91790442

Following gene: 91790440

Centisome position: 94.08

GC content: 58.86

Gene sequence:

>474_bases
ATGAACCGTCTTTCGCAAATTTTGCCCCCCTCTCAAGTGCTGGTCAGTGTTGAAGCAACCAGCAAAAAGCGTGCTTTTGA
AGAAGCCGGTCTGTTGTTTGAGAACCAGCACGGTCTGAATCGCGCGCTGATCACCGACAGCCTGTTCGCGCGTGAGCGCC
TGGGCTCGACCGGTCTTGGCCATGGCGTGGCCATTCCGCATGGCCGCATCAAGGGCCTGAAGGCCCCGATGGCCGCAGTT
TTCCAGCTGCAGGCCCCCATCGGTTTCGATGCGCCCGATGAGCAGCCCGTCGGTTTGCTGATTTTTCTGCTGGTGCCGGA
GGCCGCCACGCAAAAACACCTTGAAATCCTCTCTGAAATTGCCGAGCTGCTCAGTGACAGCACGCTGCGCGAGCAGCTCA
AGACGAGCACGGATGCTGCGGCGCTACACCACCTGATTGCATCGTGGCACTCTGTCCATGAAGTCAGCTCCTAG

Upstream 100 bases:

>100_bases
GTGTTCTGACGAGCCGCTTGTACGCGCAAGCGGCTTTTTTATGCCCCTTCTGCCTTCAAACCCGCTGTAAAGTGCATAAT
TCGGCGACTAAAAGATCGTT

Downstream 100 bases:

>100_bases
GTTGGGGCGGTTTGAGTCGTGATCTTGAGTAACAGGCCGGCATGAAGCCCACCGTTGTCAGCGCAGACGTTCTGTTTGAG
GACCACCGCGCCGCCCTGAA

Product: PTS IIA-like protein nitrogen-regulatory protein PtsN

Products: protein histidine; sugarphosphate [C]

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MNRLSQILPPSQVLVSVEATSKKRAFEEAGLLFENQHGLNRALITDSLFARERLGSTGLGHGVAIPHGRIKGLKAPMAAV
FQLQAPIGFDAPDEQPVGLLIFLLVPEAATQKHLEILSEIAELLSDSTLREQLKTSTDAAALHHLIASWHSVHEVSS

Sequences:

>Translated_157_residues
MNRLSQILPPSQVLVSVEATSKKRAFEEAGLLFENQHGLNRALITDSLFARERLGSTGLGHGVAIPHGRIKGLKAPMAAV
FQLQAPIGFDAPDEQPVGLLIFLLVPEAATQKHLEILSEIAELLSDSTLREQLKTSTDAAALHHLIASWHSVHEVSS
>Mature_157_residues
MNRLSQILPPSQVLVSVEATSKKRAFEEAGLLFENQHGLNRALITDSLFARERLGSTGLGHGVAIPHGRIKGLKAPMAAV
FQLQAPIGFDAPDEQPVGLLIFLLVPEAATQKHLEILSEIAELLSDSTLREQLKTSTDAAALHHLIASWHSVHEVSS

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=147, Percent_Identity=38.7755102040816, Blast_Score=99, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 16982; Mature: 16982

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLSQILPPSQVLVSVEATSKKRAFEEAGLLFENQHGLNRALITDSLFARERLGSTGLG
CCCHHHHCCCHHHEEEECHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHCCCCCCC
HGVAIPHGRIKGLKAPMAAVFQLQAPIGFDAPDEQPVGLLIFLLVPEAATQKHLEILSEI
CCEECCCCHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEECCCHHHHHHHHHHHHH
AELLSDSTLREQLKTSTDAAALHHLIASWHSVHEVSS
HHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNRLSQILPPSQVLVSVEATSKKRAFEEAGLLFENQHGLNRALITDSLFARERLGSTGLG
CCCHHHHCCCHHHEEEECHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHCCCCCCC
HGVAIPHGRIKGLKAPMAAVFQLQAPIGFDAPDEQPVGLLIFLLVPEAATQKHLEILSEI
CCEECCCCHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEECCCHHHHHHHHHHHHH
AELLSDSTLREQLKTSTDAAALHHLIASWHSVHEVSS
HHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein Npi-phosphohistidine; sugar [C]

Specific reaction: Protein Npi-phosphohistidine + sugar = protein histidine + sugarphosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]