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The map label for this gene is 91790412

Identifier: 91790412

GI number: 91790412

Start: 4854320

End: 4855615

Strand: Reverse

Name: 91790412

Synonym: Bpro_4583

Alternate gene names: NA

Gene position: 4855615-4854320 (Counterclockwise)

Preceding gene: 91790413

Following gene: 91790411

Centisome position: 93.37

GC content: 61.19

Gene sequence:

>1296_bases
ATGGTTCCGCATCTCATCACCGCCCTGAACGGCCCCATCAACGAACTTGAGCAGCGCATTCTTGACTCCATGCCCGCGAT
CGAGCGCTGGTTCCGGCTGGAGTGGATGGAGCACACGCCGCCCTTTTACAGCTCCGTCGATATCCGCAACGCGGGCTTCA
AGCTCGCGCCGGTGGACACCAACCTCTATCCCGGCGGCTGGAACAACCTGTCGCCCGAGATGCTGCCGCTGGCGGTGCAG
GCGGCGATGGCCGCGATTGAAAAAATCTGCCCCGAAGCCCGCAACCTGCTGGTGGTGCCCGAGAACCACACGCGCAACAG
CTTTTACCTGTCGAATGTGCTGCAACTCAAGCGCATCTTTCACCAGGCGGGCCTCAACGTGCGCTTTGGCTCGCTCAGTC
CCGAGATCAAGGAGCCCACCACGCTGAACCTGCCGACCGGCGAGACCATCACGATCGAGCCGCTGATCCGCACCGACCGG
CGGCTGGGCCTGAAAAACTTCGACCCTTGCACGATCTTGCTGAACAACGATCTGTCGGCGGGCATTCCCGGCATGCTGGA
AGACCTGCACGAGCAGTACCTGCTGCCACCGCTGCATGCCGGCTGGTCGGTACGGCGCAAGTCCAGGCACTTCAAGGCCT
ACGAAGAGGTCTCCAAGCGCTTCGGCAAGCTGCTGGGCATCGACCCCTGGCTCATCAACCCGATGTTCACGCAATGCGGT
GAAATCAACTTTGCCGAGGGCGTGGGCCTGGAGTGCCTCACCACCAACGTCGATGCGCTGCTGACCAAAATCAAGCGAAA
GTACAAGGAATACGGCATCAACGAAAAGCCCTTTGTCGTGGTCAAGGCCGACAACGGCACCTATGGCATGGGCATCATGA
CGGTGCGCGATGTGAAAGACCTTGAGGTGTTGAACCGCAAGACCAAAAACAAGATGGGCGTGACCAAGGATGGCCAGACG
CTGTCGGAAGTCATCATCCAGGAAGGCGTGCTGACCAATGAGCGCGTCAACGATGCGGTGGCCGAGCCGGTGGTCTACAT
GATGGACCGCTATGTGGTCGGCGGCTTCTACCGCGTGCATGCCGAGCGCGGCGTCGATGAAAACCTCAACGCCCCCGGCG
CCAGCTTTGTGCCGCTGGCCTTTGCCGAAAGCGGCCGCCTGCCCCAGCCTGGTGAAAAACCGGGCTCCAGCAGCCCCAAC
CGCTTCTACATGTATGGCGTGATTGCCCGGCTGGCCATGCTGGCTGCCAGCTATGAACTGGAAGCGACCGATCCGGATGC
CGAGGTCTACGACTGA

Upstream 100 bases:

>100_bases
GGCTGTGCACCTTGTAACCGCCCTGCGCATCCCAATATCCCTAGGATTCGGTCAGATATTGCCGGATTTGATAACAAAAC
TCCCCTTTGAAAGTGGCCGC

Downstream 100 bases:

>100_bases
CGGGTGCTCTGGGGTGCTGGATTGTGGGGATTGAACCCCTTGACCTGCGGAGATTGTTGGCTATAAAAATAATAGCAATT
GGGTGGCCGGGCCGAGCGGT

Product: glutamate--cysteine ligase GshA

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Glutamate-Cysteine Ligase GshA; Glutamate-Cysteine Ligase-Related Protein; GshA Protein; Glutathione Synthetase

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MVPHLITALNGPINELEQRILDSMPAIERWFRLEWMEHTPPFYSSVDIRNAGFKLAPVDTNLYPGGWNNLSPEMLPLAVQ
AAMAAIEKICPEARNLLVVPENHTRNSFYLSNVLQLKRIFHQAGLNVRFGSLSPEIKEPTTLNLPTGETITIEPLIRTDR
RLGLKNFDPCTILLNNDLSAGIPGMLEDLHEQYLLPPLHAGWSVRRKSRHFKAYEEVSKRFGKLLGIDPWLINPMFTQCG
EINFAEGVGLECLTTNVDALLTKIKRKYKEYGINEKPFVVVKADNGTYGMGIMTVRDVKDLEVLNRKTKNKMGVTKDGQT
LSEVIIQEGVLTNERVNDAVAEPVVYMMDRYVVGGFYRVHAERGVDENLNAPGASFVPLAFAESGRLPQPGEKPGSSSPN
RFYMYGVIARLAMLAASYELEATDPDAEVYD

Sequences:

>Translated_431_residues
MVPHLITALNGPINELEQRILDSMPAIERWFRLEWMEHTPPFYSSVDIRNAGFKLAPVDTNLYPGGWNNLSPEMLPLAVQ
AAMAAIEKICPEARNLLVVPENHTRNSFYLSNVLQLKRIFHQAGLNVRFGSLSPEIKEPTTLNLPTGETITIEPLIRTDR
RLGLKNFDPCTILLNNDLSAGIPGMLEDLHEQYLLPPLHAGWSVRRKSRHFKAYEEVSKRFGKLLGIDPWLINPMFTQCG
EINFAEGVGLECLTTNVDALLTKIKRKYKEYGINEKPFVVVKADNGTYGMGIMTVRDVKDLEVLNRKTKNKMGVTKDGQT
LSEVIIQEGVLTNERVNDAVAEPVVYMMDRYVVGGFYRVHAERGVDENLNAPGASFVPLAFAESGRLPQPGEKPGSSSPN
RFYMYGVIARLAMLAASYELEATDPDAEVYD
>Mature_431_residues
MVPHLITALNGPINELEQRILDSMPAIERWFRLEWMEHTPPFYSSVDIRNAGFKLAPVDTNLYPGGWNNLSPEMLPLAVQ
AAMAAIEKICPEARNLLVVPENHTRNSFYLSNVLQLKRIFHQAGLNVRFGSLSPEIKEPTTLNLPTGETITIEPLIRTDR
RLGLKNFDPCTILLNNDLSAGIPGMLEDLHEQYLLPPLHAGWSVRRKSRHFKAYEEVSKRFGKLLGIDPWLINPMFTQCG
EINFAEGVGLECLTTNVDALLTKIKRKYKEYGINEKPFVVVKADNGTYGMGIMTVRDVKDLEVLNRKTKNKMGVTKDGQT
LSEVIIQEGVLTNERVNDAVAEPVVYMMDRYVVGGFYRVHAERGVDENLNAPGASFVPLAFAESGRLPQPGEKPGSSSPN
RFYMYGVIARLAMLAASYELEATDPDAEVYD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 48292; Mature: 48292

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPHLITALNGPINELEQRILDSMPAIERWFRLEWMEHTPPFYSSVDIRNAGFKLAPVDT
CCCCHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEECCCCEEEEECCC
NLYPGGWNNLSPEMLPLAVQAAMAAIEKICPEARNLLVVPENHTRNSFYLSNVLQLKRIF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHCCEEEECCCCCCCHHHHHHHHHHHHHH
HQAGLNVRFGSLSPEIKEPTTLNLPTGETITIEPLIRTDRRLGLKNFDPCTILLNNDLSA
HHCCCEEEECCCCCCCCCCEEEECCCCCEEEECHHHHHHHHCCCCCCCCEEEEECCCCCC
GIPGMLEDLHEQYLLPPLHAGWSVRRKSRHFKAYEEVSKRFGKLLGIDPWLINPMFTQCG
CCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHCC
EINFAEGVGLECLTTNVDALLTKIKRKYKEYGINEKPFVVVKADNGTYGMGIMTVRDVKD
CCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEEECCHHH
LEVLNRKTKNKMGVTKDGQTLSEVIIQEGVLTNERVNDAVAEPVVYMMDRYVVGGFYRVH
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEE
AERGVDENLNAPGASFVPLAFAESGRLPQPGEKPGSSSPNRFYMYGVIARLAMLAASYEL
ECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCEE
EATDPDAEVYD
ECCCCCCCCCC
>Mature Secondary Structure
MVPHLITALNGPINELEQRILDSMPAIERWFRLEWMEHTPPFYSSVDIRNAGFKLAPVDT
CCCCHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEECCCCEEEEECCC
NLYPGGWNNLSPEMLPLAVQAAMAAIEKICPEARNLLVVPENHTRNSFYLSNVLQLKRIF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHCCEEEECCCCCCCHHHHHHHHHHHHHH
HQAGLNVRFGSLSPEIKEPTTLNLPTGETITIEPLIRTDRRLGLKNFDPCTILLNNDLSA
HHCCCEEEECCCCCCCCCCEEEECCCCCEEEECHHHHHHHHCCCCCCCCEEEEECCCCCC
GIPGMLEDLHEQYLLPPLHAGWSVRRKSRHFKAYEEVSKRFGKLLGIDPWLINPMFTQCG
CCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHCC
EINFAEGVGLECLTTNVDALLTKIKRKYKEYGINEKPFVVVKADNGTYGMGIMTVRDVKD
CCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEEECCHHH
LEVLNRKTKNKMGVTKDGQTLSEVIIQEGVLTNERVNDAVAEPVVYMMDRYVVGGFYRVH
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEE
AERGVDENLNAPGASFVPLAFAESGRLPQPGEKPGSSSPNRFYMYGVIARLAMLAASYEL
ECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCEE
EATDPDAEVYD
ECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA