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The map label for this gene is mhpA [H]

Identifier: 91790367

GI number: 91790367

Start: 4796363

End: 4798054

Strand: Direct

Name: mhpA [H]

Synonym: Bpro_4535

Alternate gene names: 91790367

Gene position: 4796363-4798054 (Clockwise)

Preceding gene: 91790366

Following gene: 91790368

Centisome position: 92.23

GC content: 66.13

Gene sequence:

>1692_bases
ATGAAACTGCAAGAACTGCAAGAACTTCCAAACACCCGGGGTCGCGGCGTTGATTACCAGAAGCTGGCGTTCGACTTTGA
GCGTCATGCCGACCAGGACGCCCACCCGCCCGCGCGCCACCCGGTGGTGGTGGTCGGTGCCGGTCCGGTGGGCCTGGCGC
TGGCCATCGACCTGGCACAGCAGAACGTGCCGGTAGTGCTGCTCGACAACGACTGCACGCTCTCGACCGGCTCGCGCGCC
ATCTGCTTTGCCAAACGCACGCTCGAGGTGTTCGACCGCCTCGGCTGCGGCCAGCGCATGACGGACAAGGGCGTGTCGTG
GAATGTCGGCAAGGTGTTCTTCAAGAACGATCAGATCTACAGCTTCAACCTGCTGCCCGAAGCGGGCCATGAACGGCCCG
CCTTCATCAACCTGCAGCAGTACTACGTCGAGGGCTACCTGGCCGAACGCGCGGCTGGGCTGCCGCTGATCGACCTGCGC
TGGAACAACAAGGTGGTGGGCATCGAGCAGCAGGCCGACCATGCCTTACTGAGCATCGAAACGCCCGAGGGCAACTACCA
AATCGCCGCCGGCTACGTCGCCGCCTGCGACGGCTCGCGTTCTTCGGTGCGCCAGCTCATCGGGCAGGAGAGCAAGGGCC
GAGTCTTCAAGGACCGTTTTCTCATTGCCGACGTGAAGATGAAGCTGGACCGGCCAGCCGAACGCTGGTTCTGGTTCGAC
CCCGAGTTTCATCCGAACCAGAGCGTGCTGCTGCACATGCAGCCCGACAACGTCTGGCGCATCGACTTCCAGCTCGGCTG
GGATGCCGATCCCGAGCACGAGAAAAAGCCCGAGAACATCATTCCGCGCGTCAAGGCCCTGCTGGCCAGGTCGGCGATGA
AGGACGCGCAGTTCGAGCTCGAATGGGCCAGCGTCTACACCTTCGCCTGCCTGCGCATGGACAGTTTCCGCCATGGCCGC
GTGCTGTTCGCCGGCGACTCGGCGCACGGCGTGTCGCCCTTCGGCGCCCGCGGCGCCAACTCGGGCGTGCAGGACGCCGA
GAATCTGGCGTGGAAACTGGCGGCGGTCGTGCATGGTGACGCGCCCGATGCGCTGCTCGACAGCTACGCCAGCGAGCGCG
AATACGCGGCCGACGAGAACATCCGCAATTCCACCCGCGCCACCGACTTCATCACGCCCAAGAGCGAGATCAGCCGGCTG
TTCCGCGACGCGGTGCTCGAACTGGCCAAGCGCCACGGCTTTGCGCGCACGCTGGTCAACAGCGGCCGGCTCTCGGTGCC
GTCCACGCTGCACGGCTCCGTGCTGAACACTGCCGATACCGACGTCTTCGACAGCCAGCTGGTGCCCGGCGCACCGGCCG
CCGACGCGCCTGTCGCTTGCACCGACGGCAGCGCCGGCTGGCTGCTGCGCGAACTGAACCCAGCCGGTTTCAGTGCACTG
GTGTTCGGTGACGACAGTGGTGGCGGTGATCTGGCCGAGCGCAGCGTGCGCGCGGTCACCAAGGCCGACCCAAGCGTGAA
GGTGGTGCGCATCGCGTTGAACAAAAGTCATGCACTCGCCGCGCAGCGCTACGACGCGCGGCCCGGCACCGTCTACCTGC
TGCGGCCCGACCAGCATGTTTGTGCGCGCTGGCGGCGGCCCAGCGCACAAGCCATCCGCGCCGCACTGGACCACGCACTC
GCCAAGGCATGA

Upstream 100 bases:

>100_bases
CGACGAGGCGACACAGTACCCCGACCCGCGCATCTGGACGGCCGAGCGCGACCAGCAGATGTGGGAAACGCTGGAAGGTT
GACCCCCTGAGGAGACAAAG

Downstream 100 bases:

>100_bases
AGGAGCCCGCCATGAATCTGATCACCTCCCCGAACCTCGACGCGCCCGACGATTTTTACGAAGCGCTGATCGAAGCGAAC
CAGGGACTCTCGACCGAAGA

Product: FAD-dependent oxidoreductase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase [H]

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MKLQELQELPNTRGRGVDYQKLAFDFERHADQDAHPPARHPVVVVGAGPVGLALAIDLAQQNVPVVLLDNDCTLSTGSRA
ICFAKRTLEVFDRLGCGQRMTDKGVSWNVGKVFFKNDQIYSFNLLPEAGHERPAFINLQQYYVEGYLAERAAGLPLIDLR
WNNKVVGIEQQADHALLSIETPEGNYQIAAGYVAACDGSRSSVRQLIGQESKGRVFKDRFLIADVKMKLDRPAERWFWFD
PEFHPNQSVLLHMQPDNVWRIDFQLGWDADPEHEKKPENIIPRVKALLARSAMKDAQFELEWASVYTFACLRMDSFRHGR
VLFAGDSAHGVSPFGARGANSGVQDAENLAWKLAAVVHGDAPDALLDSYASEREYAADENIRNSTRATDFITPKSEISRL
FRDAVLELAKRHGFARTLVNSGRLSVPSTLHGSVLNTADTDVFDSQLVPGAPAADAPVACTDGSAGWLLRELNPAGFSAL
VFGDDSGGGDLAERSVRAVTKADPSVKVVRIALNKSHALAAQRYDARPGTVYLLRPDQHVCARWRRPSAQAIRAALDHAL
AKA

Sequences:

>Translated_563_residues
MKLQELQELPNTRGRGVDYQKLAFDFERHADQDAHPPARHPVVVVGAGPVGLALAIDLAQQNVPVVLLDNDCTLSTGSRA
ICFAKRTLEVFDRLGCGQRMTDKGVSWNVGKVFFKNDQIYSFNLLPEAGHERPAFINLQQYYVEGYLAERAAGLPLIDLR
WNNKVVGIEQQADHALLSIETPEGNYQIAAGYVAACDGSRSSVRQLIGQESKGRVFKDRFLIADVKMKLDRPAERWFWFD
PEFHPNQSVLLHMQPDNVWRIDFQLGWDADPEHEKKPENIIPRVKALLARSAMKDAQFELEWASVYTFACLRMDSFRHGR
VLFAGDSAHGVSPFGARGANSGVQDAENLAWKLAAVVHGDAPDALLDSYASEREYAADENIRNSTRATDFITPKSEISRL
FRDAVLELAKRHGFARTLVNSGRLSVPSTLHGSVLNTADTDVFDSQLVPGAPAADAPVACTDGSAGWLLRELNPAGFSAL
VFGDDSGGGDLAERSVRAVTKADPSVKVVRIALNKSHALAAQRYDARPGTVYLLRPDQHVCARWRRPSAQAIRAALDHAL
AKA
>Mature_563_residues
MKLQELQELPNTRGRGVDYQKLAFDFERHADQDAHPPARHPVVVVGAGPVGLALAIDLAQQNVPVVLLDNDCTLSTGSRA
ICFAKRTLEVFDRLGCGQRMTDKGVSWNVGKVFFKNDQIYSFNLLPEAGHERPAFINLQQYYVEGYLAERAAGLPLIDLR
WNNKVVGIEQQADHALLSIETPEGNYQIAAGYVAACDGSRSSVRQLIGQESKGRVFKDRFLIADVKMKLDRPAERWFWFD
PEFHPNQSVLLHMQPDNVWRIDFQLGWDADPEHEKKPENIIPRVKALLARSAMKDAQFELEWASVYTFACLRMDSFRHGR
VLFAGDSAHGVSPFGARGANSGVQDAENLAWKLAAVVHGDAPDALLDSYASEREYAADENIRNSTRATDFITPKSEISRL
FRDAVLELAKRHGFARTLVNSGRLSVPSTLHGSVLNTADTDVFDSQLVPGAPAADAPVACTDGSAGWLLRELNPAGFSAL
VFGDDSGGGDLAERSVRAVTKADPSVKVVRIALNKSHALAAQRYDARPGTVYLLRPDQHVCARWRRPSAQAIRAALDHAL
AKA

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=380, Percent_Identity=28.9473684210526, Blast_Score=135, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 62038; Mature: 62038

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQELQELPNTRGRGVDYQKLAFDFERHADQDAHPPARHPVVVVGAGPVGLALAIDLAQ
CCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEEEHHH
QNVPVVLLDNDCTLSTGSRAICFAKRTLEVFDRLGCGQRMTDKGVSWNVGKVFFKNDQIY
CCCCEEEECCCCEECCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCEEE
SFNLLPEAGHERPAFINLQQYYVEGYLAERAAGLPLIDLRWNNKVVGIEQQADHALLSIE
EEECCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCCCCEEEEEE
TPEGNYQIAAGYVAACDGSRSSVRQLIGQESKGRVFKDRFLIADVKMKLDRPAERWFWFD
CCCCCEEEEEEEEEECCCCHHHHHHHHCCCCCCCEECCEEEEEEEEEECCCCHHHEEEEC
PEFHPNQSVLLHMQPDNVWRIDFQLGWDADPEHEKKPENIIPRVKALLARSAMKDAQFEL
CCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
EWASVYTFACLRMDSFRHGRVLFAGDSAHGVSPFGARGANSGVQDAENLAWKLAAVVHGD
EHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHEEEEEEEECC
APDALLDSYASEREYAADENIRNSTRATDFITPKSEISRLFRDAVLELAKRHGFARTLVN
CCHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHC
SGRLSVPSTLHGSVLNTADTDVFDSQLVPGAPAADAPVACTDGSAGWLLRELNPAGFSAL
CCCCCCCCCCCCCEECCCCCCHHCCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCEEEE
VFGDDSGGGDLAERSVRAVTKADPSVKVVRIALNKSHALAAQRYDARPGTVYLLRPDQHV
EEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHCCCCCCEEEEECCCHHH
CARWRRPSAQAIRAALDHALAKA
HHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLQELQELPNTRGRGVDYQKLAFDFERHADQDAHPPARHPVVVVGAGPVGLALAIDLAQ
CCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEEEHHH
QNVPVVLLDNDCTLSTGSRAICFAKRTLEVFDRLGCGQRMTDKGVSWNVGKVFFKNDQIY
CCCCEEEECCCCEECCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCEEE
SFNLLPEAGHERPAFINLQQYYVEGYLAERAAGLPLIDLRWNNKVVGIEQQADHALLSIE
EEECCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCCCCEEEEEE
TPEGNYQIAAGYVAACDGSRSSVRQLIGQESKGRVFKDRFLIADVKMKLDRPAERWFWFD
CCCCCEEEEEEEEEECCCCHHHHHHHHCCCCCCCEECCEEEEEEEEEECCCCHHHEEEEC
PEFHPNQSVLLHMQPDNVWRIDFQLGWDADPEHEKKPENIIPRVKALLARSAMKDAQFEL
CCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
EWASVYTFACLRMDSFRHGRVLFAGDSAHGVSPFGARGANSGVQDAENLAWKLAAVVHGD
EHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHEEEEEEEECC
APDALLDSYASEREYAADENIRNSTRATDFITPKSEISRLFRDAVLELAKRHGFARTLVN
CCHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHC
SGRLSVPSTLHGSVLNTADTDVFDSQLVPGAPAADAPVACTDGSAGWLLRELNPAGFSAL
CCCCCCCCCCCCCEECCCCCCHHCCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCEEEE
VFGDDSGGGDLAERSVRAVTKADPSVKVVRIALNKSHALAAQRYDARPGTVYLLRPDQHV
EEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHCCCCCCEEEEECCCHHH
CARWRRPSAQAIRAALDHALAKA
HHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA