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The map label for this gene is potD [C]

Identifier: 91790253

GI number: 91790253

Start: 4665793

End: 4666821

Strand: Reverse

Name: potD [C]

Synonym: Bpro_4420

Alternate gene names: 91790253

Gene position: 4666821-4665793 (Counterclockwise)

Preceding gene: 91790254

Following gene: 91790252

Centisome position: 89.74

GC content: 54.62

Gene sequence:

>1029_bases
ATGAGACAAATTAAAGCATTCTGTTTTGCTCTTGCATTAATTGGCGCGGGCCATTCGGTGGCGCAACAGCCCACCCTTTA
TGTGGGTGGTCCTGGTGGCAGTGCGCAAAAGCTATTCCAAGAAAGAATCATTCCTGCCTTTGAGGCCACCCACAAAGTCA
AGATCACCTATGTTCCTGGTATATCCAGCGAACTGGTCGCCAAGTTGCAAGCCCAACGTGGCAAGCAGGAGATGAATGTG
GTCATAGTTGACGACGGTCCGATGTACCAGGCCATCCAGTATGGTTACTGCCAACCTCTTACAAACGCTCCCGTCTATTC
CGACGTTTACGAAACGGCCCGTTTTGGTGGTAACGCCATCGGTGTGGGCTTGGTCGCCACGGGTATCGCCTACAACAAAG
AGGCGTTCGCGAAAAAAGGCTGGAAGGCGCCGGTGTCATGGCAAGACCTGACCGACCCAAAGTACAACCAGCGCTTCACG
ACATCGTCATTGTCTGGCACCTACGGCGTTCACACCTTGGTTATGTTTGCGCGCGCGAACGGCGGAAGTGAAAAAAATAT
CGAGCCCGGTTTTGACGTCATTGGAAAAAAACTGGCCCCCAATGTGTTGAGCTGGTCTTCATCGCCGGCCAAGCTCGCCG
AGATGTTCCAGAATCGCGATGTGGACGTGGCGATCTGGGGTAGTAGTCGCGTATATGCATTGAAAAAGACCGGCTTCCCG
ATCGAGTTCGTTTATCCGAAGGAGGGGGCTGCAGCCCTGGTGCTGGCGGCCTGCCCGGTCGTGCAAAACAGTGCGCCGGA
ACAGTCTCAGGCCTTCGTGCAGCACCTGGTTTCACCGCAAGTGCAGCAATGGCTAGCTGAAGAGGGCTTTGGCCCCACCA
ATAGCAAAACCAAGCTCGAGGGAGAAATTGCCAAAAACGTGCCGTACGGGCCGGACTTCATCAAGAAGCTTGCCAAGGTC
GACTGGGATGTCGTGAACCAGAAGCGCGCCGAATGGACCAACCGCTGGAACCGCACCATAGAACGTTGA

Upstream 100 bases:

>100_bases
CTACACGTTGTGAACTGGTTGATGGCTTAGAACCCAGCGTGAGGCTACTTTCCCCGCCAGCCCTGGTAGGGACCCACAAA
TTCTATAAAGCGGTGAAATG

Downstream 100 bases:

>100_bases
TCCTTTTGTACCCGCTTTTATTCCCTTTCTGGAGGGGGACCCTGTCCCCACATGTCCCATGAGCTTTTTATCACTCCGAG
CTTTGTCCAAGACGTACGGC

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: Twin-Arginine Translocation Pathway Signal; ABC Transporter

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MRQIKAFCFALALIGAGHSVAQQPTLYVGGPGGSAQKLFQERIIPAFEATHKVKITYVPGISSELVAKLQAQRGKQEMNV
VIVDDGPMYQAIQYGYCQPLTNAPVYSDVYETARFGGNAIGVGLVATGIAYNKEAFAKKGWKAPVSWQDLTDPKYNQRFT
TSSLSGTYGVHTLVMFARANGGSEKNIEPGFDVIGKKLAPNVLSWSSSPAKLAEMFQNRDVDVAIWGSSRVYALKKTGFP
IEFVYPKEGAAALVLAACPVVQNSAPEQSQAFVQHLVSPQVQQWLAEEGFGPTNSKTKLEGEIAKNVPYGPDFIKKLAKV
DWDVVNQKRAEWTNRWNRTIER

Sequences:

>Translated_342_residues
MRQIKAFCFALALIGAGHSVAQQPTLYVGGPGGSAQKLFQERIIPAFEATHKVKITYVPGISSELVAKLQAQRGKQEMNV
VIVDDGPMYQAIQYGYCQPLTNAPVYSDVYETARFGGNAIGVGLVATGIAYNKEAFAKKGWKAPVSWQDLTDPKYNQRFT
TSSLSGTYGVHTLVMFARANGGSEKNIEPGFDVIGKKLAPNVLSWSSSPAKLAEMFQNRDVDVAIWGSSRVYALKKTGFP
IEFVYPKEGAAALVLAACPVVQNSAPEQSQAFVQHLVSPQVQQWLAEEGFGPTNSKTKLEGEIAKNVPYGPDFIKKLAKV
DWDVVNQKRAEWTNRWNRTIER
>Mature_342_residues
MRQIKAFCFALALIGAGHSVAQQPTLYVGGPGGSAQKLFQERIIPAFEATHKVKITYVPGISSELVAKLQAQRGKQEMNV
VIVDDGPMYQAIQYGYCQPLTNAPVYSDVYETARFGGNAIGVGLVATGIAYNKEAFAKKGWKAPVSWQDLTDPKYNQRFT
TSSLSGTYGVHTLVMFARANGGSEKNIEPGFDVIGKKLAPNVLSWSSSPAKLAEMFQNRDVDVAIWGSSRVYALKKTGFP
IEFVYPKEGAAALVLAACPVVQNSAPEQSQAFVQHLVSPQVQQWLAEEGFGPTNSKTKLEGEIAKNVPYGPDFIKKLAKV
DWDVVNQKRAEWTNRWNRTIER

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 37509; Mature: 37509

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQIKAFCFALALIGAGHSVAQQPTLYVGGPGGSAQKLFQERIIPAFEATHKVKITYVPG
CCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHCCHHHCCCEEEEEEECCC
ISSELVAKLQAQRGKQEMNVVIVDDGPMYQAIQYGYCQPLTNAPVYSDVYETARFGGNAI
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEE
GVGLVATGIAYNKEAFAKKGWKAPVSWQDLTDPKYNQRFTTSSLSGTYGVHTLVMFARAN
EHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHEEECC
GGSEKNIEPGFDVIGKKLAPNVLSWSSSPAKLAEMFQNRDVDVAIWGSSRVYALKKTGFP
CCCCCCCCCCHHHHHHHHCCHHHCCCCCHHHHHHHHHCCCCEEEEECCCEEEEEECCCCC
IEFVYPKEGAAALVLAACPVVQNSAPEQSQAFVQHLVSPQVQQWLAEEGFGPTNSKTKLE
EEEEECCCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCEEC
GEIAKNVPYGPDFIKKLAKVDWDVVNQKRAEWTNRWNRTIER
CHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRQIKAFCFALALIGAGHSVAQQPTLYVGGPGGSAQKLFQERIIPAFEATHKVKITYVPG
CCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHCCHHHCCCEEEEEEECCC
ISSELVAKLQAQRGKQEMNVVIVDDGPMYQAIQYGYCQPLTNAPVYSDVYETARFGGNAI
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEE
GVGLVATGIAYNKEAFAKKGWKAPVSWQDLTDPKYNQRFTTSSLSGTYGVHTLVMFARAN
EHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHEEECC
GGSEKNIEPGFDVIGKKLAPNVLSWSSSPAKLAEMFQNRDVDVAIWGSSRVYALKKTGFP
CCCCCCCCCCHHHHHHHHCCHHHCCCCCHHHHHHHHHCCCCEEEEECCCEEEEEECCCCC
IEFVYPKEGAAALVLAACPVVQNSAPEQSQAFVQHLVSPQVQQWLAEEGFGPTNSKTKLE
EEEEECCCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCEEC
GEIAKNVPYGPDFIKKLAKVDWDVVNQKRAEWTNRWNRTIER
CHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA