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The map label for this gene is ooxB [H]

Identifier: 91790249

GI number: 91790249

Start: 4661759

End: 4662895

Strand: Reverse

Name: ooxB [H]

Synonym: Bpro_4416

Alternate gene names: 91790249

Gene position: 4662895-4661759 (Counterclockwise)

Preceding gene: 91790250

Following gene: 91790248

Centisome position: 89.67

GC content: 60.16

Gene sequence:

>1137_bases
ATGCAAACACAAGAATTGGTAGTGATTGGCGGGGGCTTGGTCGGCGCTGCCATCGCCTACGGGGCCGCCCGGGCCGGCGC
CCCCGTGACCCTGCTTGACCAAGGCGATATCGCACATCGCGCCTCACGCGGAAATTTTGGTCTGGTGTGGGTGCAGAGCA
AAGGCGACAAGGTGCCGCGCTATGCGCGCTGGAGCCGTGACGCTCTGACATTTTGGCCCGCGCTGGCCAAAGAGTTGCTC
GAACTCACCGGTGTTGACGTAGCCCTGCACCAGCCCGGAGGCTTCTGGATTGGTTTTAGCGAAGCCGAGATGCAGGCGCG
TGAGGCCATGCTGGAACGAATCAATAGCTCAGTAGGGGGCATTCCCTATGAAATGCTCGACCACGCCGCGCTCAAGGCGC
GCCTTCCGGCCATTGGTCCTACGGTCGCGGGAGGCAGCTACTGCCCGCTAGACGGTCATGCTAATCCGCTCATGCTCTTG
CACGCCCTCCATGCCGGCCTTAAGGCACGCGGTGCGCGAGTGGTCTCTAGTGTTGATGTCGCGCAAATCACATACCAGGA
GAAAGGCACTGGCTTTGAAATCAAGAGCAAGGACGGAAAGAGCTGGCACGCCCCGCGCGTTGTACTCGCCGCTGGCTTGG
GCAACAACGAACTCGCTCCTCAGGTCGGCCTGCATGCACCGGTTTTGCCGAACAGAGGGCAAGTTCTCATTACCGAAAGG
CTCAAGCCTTTTTTGCCCTATCCCACCAACAAGGCAAGGCAAACCAACGAAGGCAGTGTCCAGTTGGGTTATTCGGTCGA
AGACGTCGGACTGGATGACAGCACCACAGTCTCTGCCGTTGAATGGATCGCCAAACGCGCGGTGACTACCTTTCCCATTC
TTTCCAGCGTGCGCCTCGTCCGCGCCTGGGGTGCCTTGCGGGTACTGACACCGGATGGCCTGCCGATCTACCAAGAGTCG
GTGTCGTGTCCAGGCGCATTCGTGGCGACCAGTCATAGCGGCGTATCGCTAGCGGCCGCCCATGCTCACGTGATTGGACC
TTGGGTGGCCGGGCTGGGTGCCGCGCCTGATGCCTTCGAAGTATTTGCAGGGGAACGATTTCTGGACCCCAACCGGAGCT
TTTCAAATGCACACTAA

Upstream 100 bases:

>100_bases
CCCTTTCTCTTTAACAGAAACATTAACAGGCCAAGCCTGTGAAGGACGGCGCTCGTCCCGACGAACGCCAAAGCCCCACA
TCGCAGGCAAGGAGAACATC

Downstream 100 bases:

>100_bases
AACCACTTCTCCTTGCCCTGCGCTATTTACATCGAACGGGGATTCCCCTTCAGACCCGGTCACGCTGCGATTCGATGACC
GCACGATTACCGCTCCCGCG

Product: FAD dependent oxidoreductase

Products: Dihydroxyacetone phosphate; Ubiquinol-8 [C]

Alternate protein names: Octopine oxidase subunit B [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MQTQELVVIGGGLVGAAIAYGAARAGAPVTLLDQGDIAHRASRGNFGLVWVQSKGDKVPRYARWSRDALTFWPALAKELL
ELTGVDVALHQPGGFWIGFSEAEMQAREAMLERINSSVGGIPYEMLDHAALKARLPAIGPTVAGGSYCPLDGHANPLMLL
HALHAGLKARGARVVSSVDVAQITYQEKGTGFEIKSKDGKSWHAPRVVLAAGLGNNELAPQVGLHAPVLPNRGQVLITER
LKPFLPYPTNKARQTNEGSVQLGYSVEDVGLDDSTTVSAVEWIAKRAVTTFPILSSVRLVRAWGALRVLTPDGLPIYQES
VSCPGAFVATSHSGVSLAAAHAHVIGPWVAGLGAAPDAFEVFAGERFLDPNRSFSNAH

Sequences:

>Translated_378_residues
MQTQELVVIGGGLVGAAIAYGAARAGAPVTLLDQGDIAHRASRGNFGLVWVQSKGDKVPRYARWSRDALTFWPALAKELL
ELTGVDVALHQPGGFWIGFSEAEMQAREAMLERINSSVGGIPYEMLDHAALKARLPAIGPTVAGGSYCPLDGHANPLMLL
HALHAGLKARGARVVSSVDVAQITYQEKGTGFEIKSKDGKSWHAPRVVLAAGLGNNELAPQVGLHAPVLPNRGQVLITER
LKPFLPYPTNKARQTNEGSVQLGYSVEDVGLDDSTTVSAVEWIAKRAVTTFPILSSVRLVRAWGALRVLTPDGLPIYQES
VSCPGAFVATSHSGVSLAAAHAHVIGPWVAGLGAAPDAFEVFAGERFLDPNRSFSNAH
>Mature_378_residues
MQTQELVVIGGGLVGAAIAYGAARAGAPVTLLDQGDIAHRASRGNFGLVWVQSKGDKVPRYARWSRDALTFWPALAKELL
ELTGVDVALHQPGGFWIGFSEAEMQAREAMLERINSSVGGIPYEMLDHAALKARLPAIGPTVAGGSYCPLDGHANPLMLL
HALHAGLKARGARVVSSVDVAQITYQEKGTGFEIKSKDGKSWHAPRVVLAAGLGNNELAPQVGLHAPVLPNRGQVLITER
LKPFLPYPTNKARQTNEGSVQLGYSVEDVGLDDSTTVSAVEWIAKRAVTTFPILSSVRLVRAWGALRVLTPDGLPIYQES
VSCPGAFVATSHSGVSLAAAHAHVIGPWVAGLGAAPDAFEVFAGERFLDPNRSFSNAH

Specific function: Oxidative cleavage of octopine into L-arginine and pyruvate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Loosely Bound To The Cytoplasm Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI24797151, Length=401, Percent_Identity=25.4364089775561, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI20130091, Length=240, Percent_Identity=26.25, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.1.99.5 [C]

Molecular weight: Translated: 40078; Mature: 40078

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTQELVVIGGGLVGAAIAYGAARAGAPVTLLDQGDIAHRASRGNFGLVWVQSKGDKVPR
CCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCCEEEEEEECCCCCCCC
YARWSRDALTFWPALAKELLELTGVDVALHQPGGFWIGFSEAEMQAREAMLERINSSVGG
HHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHCCC
IPYEMLDHAALKARLPAIGPTVAGGSYCPLDGHANPLMLLHALHAGLKARGARVVSSVDV
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCE
AQITYQEKGTGFEIKSKDGKSWHAPRVVLAAGLGNNELAPQVGLHAPVLPNRGQVLITER
EEEEEECCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECC
LKPFLPYPTNKARQTNEGSVQLGYSVEDVGLDDSTTVSAVEWIAKRAVTTFPILSSVRLV
CCCCCCCCCCCCCCCCCCCEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RAWGALRVLTPDGLPIYQESVSCPGAFVATSHSGVSLAAAHAHVIGPWVAGLGAAPDAFE
HHCCCEEEECCCCCCHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCHHH
VFAGERFLDPNRSFSNAH
HHCCCEECCCCCCCCCCC
>Mature Secondary Structure
MQTQELVVIGGGLVGAAIAYGAARAGAPVTLLDQGDIAHRASRGNFGLVWVQSKGDKVPR
CCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCCEEEEEEECCCCCCCC
YARWSRDALTFWPALAKELLELTGVDVALHQPGGFWIGFSEAEMQAREAMLERINSSVGG
HHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHCCC
IPYEMLDHAALKARLPAIGPTVAGGSYCPLDGHANPLMLLHALHAGLKARGARVVSSVDV
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCE
AQITYQEKGTGFEIKSKDGKSWHAPRVVLAAGLGNNELAPQVGLHAPVLPNRGQVLITER
EEEEEECCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECC
LKPFLPYPTNKARQTNEGSVQLGYSVEDVGLDDSTTVSAVEWIAKRAVTTFPILSSVRLV
CCCCCCCCCCCCCCCCCCCEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RAWGALRVLTPDGLPIYQESVSCPGAFVATSHSGVSLAAAHAHVIGPWVAGLGAAPDAFE
HHCCCEEEECCCCCCHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCHHH
VFAGERFLDPNRSFSNAH
HHCCCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glycerol 3-phosphate; Ubiquinone-8 [C]

Specific reaction: Glycerol 3-phosphate + Ubiquinone-8 --> Dihydroxyacetone phosphate + Ubiquinol-8 [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]