Click here to switch to the map view.

The map label for this gene is potB [H]

Identifier: 91790213

GI number: 91790213

Start: 4619751

End: 4620668

Strand: Reverse

Name: potB [H]

Synonym: Bpro_4378

Alternate gene names: 91790213

Gene position: 4620668-4619751 (Counterclockwise)

Preceding gene: 91790214

Following gene: 91790212

Centisome position: 88.85

GC content: 66.45

Gene sequence:

>918_bases
ATGGCTGAAGCGACTGCCGCCCTGTCCGCGGCCGGCGCTGTGCCGCGCCGCAAATCCACCGAAGGCGCGGGCGCCGCGCC
CTGGCTGCTGGCATCGCCCGCGCTGCTGCTGTTCATCGGCTTGCTGCTGGTGCCGCTGCTGTTGACGGCGCTGCTGTCCT
TCCACGCCTTCGACGGCACGCGCGGCGTGCTTCCCGACTACACCTGGCGCAACTACCTCGAGGTGTTCTCCGATGGTTAT
TACCACGAGATCTTCCTGCGCACCGGCGGCCTGGCGCTGGGCGTGACCTTGTTGTGCATGGTCTTGGGCGTGCCGGAGAC
GCTGATCCTGGCGCGCATGAAAAGCCCCTGGCGCGCGCTCTTCCTCATCGTCATCCTCGGCCCCTTGCTGATCTCGGTGG
TGGTGCGCACGCTGGGCTGGGCCATCCTCTTCGGCAACAACGGGCTGATCAACAGCACGCTGATGGCCCTTGGCATCACC
AGCGAGCCGGTACGCCTGCTTTTCAGCATGACGGGCGTGGCCATCGCGCTGACCCACGTGCTGATTCCCTTCATGGTGAT
CTCGGTCTGGGCCAACCTGCAAAAGCTTGATCCGCAGGTGGAAAACGCCGGCCTTTCGCTGGGCGCCCCGCCGCTCAAGG
TGTTCTGGCGCGTGATGCTGCCGCAGCTGCTGCCCGGCATCCTCTCGGGCTGCATCATCGTCTTCGCCCTGTCGGCTTCG
GCCTTCGCCACGCCGGCCCTGCTGGGCGGGCGCAAGCTCAAGGTGGTCGCCACGGCCGCTTATGACGAGTTCCTCAACAC
GCTGAACTGGCCGCTGGGAGCCACCATCGCCATGCTGCTGCTGTTGGCCAACGTGCTCATCATCCTCGGCGCCAACCGCT
GGGTGGAGCGCCGCTTCAGCCAGGTCTTCGACGCCTGA

Upstream 100 bases:

>100_bases
CTGCCCAACATCGGCGAAGTGCCGCCGGCCGAAGGCAGCACCGTCGGCGTGAGCTGGTCGGACGACGACCTGCGCGTGCT
GCCCCTGCAGGAGGTGGCTC

Downstream 100 bases:

>100_bases
GCACCCCAGAACATCCAAGGACTTCCCCATGCGCAAGAACGGTTTCTTCTCATTGCTCTACCACGCCCTGTTCATCGGCT
TCATCATCGCGCCACTGGTG

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MAEATAALSAAGAVPRRKSTEGAGAAPWLLASPALLLFIGLLLVPLLLTALLSFHAFDGTRGVLPDYTWRNYLEVFSDGY
YHEIFLRTGGLALGVTLLCMVLGVPETLILARMKSPWRALFLIVILGPLLISVVVRTLGWAILFGNNGLINSTLMALGIT
SEPVRLLFSMTGVAIALTHVLIPFMVISVWANLQKLDPQVENAGLSLGAPPLKVFWRVMLPQLLPGILSGCIIVFALSAS
AFATPALLGGRKLKVVATAAYDEFLNTLNWPLGATIAMLLLLANVLIILGANRWVERRFSQVFDA

Sequences:

>Translated_305_residues
MAEATAALSAAGAVPRRKSTEGAGAAPWLLASPALLLFIGLLLVPLLLTALLSFHAFDGTRGVLPDYTWRNYLEVFSDGY
YHEIFLRTGGLALGVTLLCMVLGVPETLILARMKSPWRALFLIVILGPLLISVVVRTLGWAILFGNNGLINSTLMALGIT
SEPVRLLFSMTGVAIALTHVLIPFMVISVWANLQKLDPQVENAGLSLGAPPLKVFWRVMLPQLLPGILSGCIIVFALSAS
AFATPALLGGRKLKVVATAAYDEFLNTLNWPLGATIAMLLLLANVLIILGANRWVERRFSQVFDA
>Mature_304_residues
AEATAALSAAGAVPRRKSTEGAGAAPWLLASPALLLFIGLLLVPLLLTALLSFHAFDGTRGVLPDYTWRNYLEVFSDGYY
HEIFLRTGGLALGVTLLCMVLGVPETLILARMKSPWRALFLIVILGPLLISVVVRTLGWAILFGNNGLINSTLMALGITS
EPVRLLFSMTGVAIALTHVLIPFMVISVWANLQKLDPQVENAGLSLGAPPLKVFWRVMLPQLLPGILSGCIIVFALSASA
FATPALLGGRKLKVVATAAYDEFLNTLNWPLGATIAMLLLLANVLIILGANRWVERRFSQVFDA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=249, Percent_Identity=31.7269076305221, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1787080, Length=231, Percent_Identity=28.5714285714286, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787713, Length=211, Percent_Identity=31.7535545023697, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788764, Length=220, Percent_Identity=26.3636363636364, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32785; Mature: 32654

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEATAALSAAGAVPRRKSTEGAGAAPWLLASPALLLFIGLLLVPLLLTALLSFHAFDGT
CCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
RGVLPDYTWRNYLEVFSDGYYHEIFLRTGGLALGVTLLCMVLGVPETLILARMKSPWRAL
CCCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
FLIVILGPLLISVVVRTLGWAILFGNNGLINSTLMALGITSEPVRLLFSMTGVAIALTHV
HHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LIPFMVISVWANLQKLDPQVENAGLSLGAPPLKVFWRVMLPQLLPGILSGCIIVFALSAS
HHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFATPALLGGRKLKVVATAAYDEFLNTLNWPLGATIAMLLLLANVLIILGANRWVERRFS
HHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QVFDA
HHHCC
>Mature Secondary Structure 
AEATAALSAAGAVPRRKSTEGAGAAPWLLASPALLLFIGLLLVPLLLTALLSFHAFDGT
CCHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
RGVLPDYTWRNYLEVFSDGYYHEIFLRTGGLALGVTLLCMVLGVPETLILARMKSPWRAL
CCCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
FLIVILGPLLISVVVRTLGWAILFGNNGLINSTLMALGITSEPVRLLFSMTGVAIALTHV
HHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LIPFMVISVWANLQKLDPQVENAGLSLGAPPLKVFWRVMLPQLLPGILSGCIIVFALSAS
HHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFATPALLGGRKLKVVATAAYDEFLNTLNWPLGATIAMLLLLANVLIILGANRWVERRFS
HHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QVFDA
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]