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The map label for this gene is potB [H]

Identifier: 91790188

GI number: 91790188

Start: 4591799

End: 4592620

Strand: Reverse

Name: potB [H]

Synonym: Bpro_4353

Alternate gene names: 91790188

Gene position: 4592620-4591799 (Counterclockwise)

Preceding gene: 91790189

Following gene: 91790187

Centisome position: 88.32

GC content: 57.06

Gene sequence:

>822_bases
ATGAAGCGATATGGCGCTTGGCCCGCATGGCTGTTCATGCTGGCCTTCTTTTTCCTACCCTTGGCAGCACTGGTGCCAGA
GGCAATGACAGACGGCGGAACCGCGTTCTCGAGAATATTTTCGGACAACCTGTTCCTGGCCGCTACGCGCAACACGATCG
TGCTTGGATTACTTGCCGGCGGATTGTCCGCCGTAGTGGGTACGGCAATCGCTATCGAGCTGGCGCGGCAGCCTGAGCGG
CGTCGTCAGTGGATGATGATGCTGTTGGGACTACCACTGGCATTCTCCGGTTTGGTGATCGCTTACGGGTTCATCCTGGC
TTTCGGACGTGCCGGTTTTGTTACGCAGATCCTAGCATTCCTAGGAGCGGATCCCGCTCGCGTCGGCGGTTGGATTTACA
CGGTCTCCGGTCTGGGCTTTGCATACGCCTATTACCTGATACCTCGTGTGGCGCTCTCGCTCTATCCTGTGTTCGCAAAT
TTTGACGAGCGGCCTTTGCTAGCTGCGCGCACCTTGGGGGCTTCGAAATTTCGAGCATTTGTCGACACGGTCATCCCTGA
AGTCGCGCCAACTGTCCTGGCCTCAGCTTGCATGGTGGCCGCCCTGGCGATGGGTACGTACGGGACAGCCCTGGCTTTGG
TCGGCACTCAGCTGAACATACTTCCCTTGATGTTGCTTTCGAAGGTGAGCGATGGTGGCTCAGACTTCCCTGCTGCGGCT
GCCATGTCGCTGGTCTTGATGGCGATTTGCCTTATTGTTTTAGGAGTCGGTGATGTCATCACAAGTAAGCGCGAGCGAAG
CATGCTCGACGCGAGTCATTGA

Upstream 100 bases:

>100_bases
CCCGCTTGATGTTGTAAAGGCGTCCGAGCGCAAGGCGGAGATTGACCGCCTTTGGGCACAAGCTGTTCTTACCAAGTAAT
AACCCTTAAGCGGTTTGACT

Downstream 100 bases:

>100_bases
GTTGCTTGGGCGACTCTCAGCGATCCAGCGAAGTCCGAAGCGCTGTATCCAGCCTGTGGGAATTATTGGTCCGGGTGACG
GTGGGACTCGTGAATGCGAA

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKRYGAWPAWLFMLAFFFLPLAALVPEAMTDGGTAFSRIFSDNLFLAATRNTIVLGLLAGGLSAVVGTAIAIELARQPER
RRQWMMMLLGLPLAFSGLVIAYGFILAFGRAGFVTQILAFLGADPARVGGWIYTVSGLGFAYAYYLIPRVALSLYPVFAN
FDERPLLAARTLGASKFRAFVDTVIPEVAPTVLASACMVAALAMGTYGTALALVGTQLNILPLMLLSKVSDGGSDFPAAA
AMSLVLMAICLIVLGVGDVITSKRERSMLDASH

Sequences:

>Translated_273_residues
MKRYGAWPAWLFMLAFFFLPLAALVPEAMTDGGTAFSRIFSDNLFLAATRNTIVLGLLAGGLSAVVGTAIAIELARQPER
RRQWMMMLLGLPLAFSGLVIAYGFILAFGRAGFVTQILAFLGADPARVGGWIYTVSGLGFAYAYYLIPRVALSLYPVFAN
FDERPLLAARTLGASKFRAFVDTVIPEVAPTVLASACMVAALAMGTYGTALALVGTQLNILPLMLLSKVSDGGSDFPAAA
AMSLVLMAICLIVLGVGDVITSKRERSMLDASH
>Mature_273_residues
MKRYGAWPAWLFMLAFFFLPLAALVPEAMTDGGTAFSRIFSDNLFLAATRNTIVLGLLAGGLSAVVGTAIAIELARQPER
RRQWMMMLLGLPLAFSGLVIAYGFILAFGRAGFVTQILAFLGADPARVGGWIYTVSGLGFAYAYYLIPRVALSLYPVFAN
FDERPLLAARTLGASKFRAFVDTVIPEVAPTVLASACMVAALAMGTYGTALALVGTQLNILPLMLLSKVSDGGSDFPAAA
AMSLVLMAICLIVLGVGDVITSKRERSMLDASH

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=207, Percent_Identity=28.5024154589372, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29043; Mature: 29043

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRYGAWPAWLFMLAFFFLPLAALVPEAMTDGGTAFSRIFSDNLFLAATRNTIVLGLLAG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEECCHHHHHHHHH
GLSAVVGTAIAIELARQPERRRQWMMMLLGLPLAFSGLVIAYGFILAFGRAGFVTQILAF
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LGADPARVGGWIYTVSGLGFAYAYYLIPRVALSLYPVFANFDERPLLAARTLGASKFRAF
HCCCHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VDTVIPEVAPTVLASACMVAALAMGTYGTALALVGTQLNILPLMLLSKVSDGGSDFPAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHH
AMSLVLMAICLIVLGVGDVITSKRERSMLDASH
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKRYGAWPAWLFMLAFFFLPLAALVPEAMTDGGTAFSRIFSDNLFLAATRNTIVLGLLAG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEECCHHHHHHHHH
GLSAVVGTAIAIELARQPERRRQWMMMLLGLPLAFSGLVIAYGFILAFGRAGFVTQILAF
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LGADPARVGGWIYTVSGLGFAYAYYLIPRVALSLYPVFANFDERPLLAARTLGASKFRAF
HCCCHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VDTVIPEVAPTVLASACMVAALAMGTYGTALALVGTQLNILPLMLLSKVSDGGSDFPAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHH
AMSLVLMAICLIVLGVGDVITSKRERSMLDASH
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]