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The map label for this gene is gsiB [H]

Identifier: 91790170

GI number: 91790170

Start: 4572241

End: 4573821

Strand: Reverse

Name: gsiB [H]

Synonym: Bpro_4335

Alternate gene names: 91790170

Gene position: 4573821-4572241 (Counterclockwise)

Preceding gene: 91790171

Following gene: 91790168

Centisome position: 87.95

GC content: 58.06

Gene sequence:

>1581_bases
ATGAAGGCGGTCGACTATGTGATCGCGTGTCGAGCGCTCCTCATCGCCGCATGTATTGGCCTTGTCGGGCCGGTATTTGC
TCAGGAGTCCATCCTCCGCATCGGAAGTGATGCGATTGACATCACTACTCTGGATCCGCACCGCACGGCGGTGTCGGAAG
AAAAGGGCTTGATTAGCATGATCTTCGGTGGCTTGGTGAGATTTCCACCCGGTTCCGCTGACCCCGAGAAAATAGAAGGT
GATTTGGCCGAGAGCTGGCAGGCTTCTGCTGATGGACTGACCTGGACATTCAAGCTGCGTCACGGTGTGCAGTTCCACCG
CAACTATGGCGAGGTGAATGCGGAGGATGTGGTTTATAGCCTAATGCGCGCAGCCGATCCAAAGCGGTCCTCGTTTTCGT
CTACCTTTGAGCCGGTGAAGGAAGTGAGCGCGCTCGATTCGCATACGGTGCGCATACAGCTCAAGGCGCCAGTACCCAGT
CTGCTGGGACTGGTTGCCAACTACCACGGCGGAATGGTCGTAAGCCGCAAGGCCGACCAGGATCTGAAAGACGCGTTCAA
ATTGAAGCCAGTTGGCTTTGGGCCCTTCGAGTTCGTTGAACATCAGAAACAGCGCGAGGTGGTGCTGAAGGCGCACGACA
AGTATTTCCGTGGGAAGCCGAAGATAGAGCGCATCGTTTACCGATTCATCCCCTCCGAGGCCGTCCGGGGGCTAGCGTTC
GCTACTGGCGAGCTTGACCTGGTCGCCGGCCAGCGCGATCAGCGCTGGGTGCAGCGGGCCCGGCTCTGGGCGCCGCCTAA
GGACCAATCGTCCGTCAAAGTCGATGTCTTCGGACCAGGTGAATTTCGTACCCTGATGCTCAATCGGCGCATCAAGCCGC
TGGACGACCCGCGTGTTCGGGAAGCCGTAGCACGCGCCGTGGATGTGCAGGAACTAGTTCACTTCGTCGGCGCGGATATC
GTCAAGCCTGGCAGATCTGTCATTCCGCCAGGCTACGCAGGTGAGGTAGACGTTGGGCCAAAGTTTCCTTATAACGTCGG
CAAGTCAAAAGCTTTGCTGACGGAAGCCGGCTACGCCAACGGCATCACCCTAAGGGCGGTAGTGTCCAGTACCGCCTCGC
AACTGTCGGTTATGGATGTGGTGCAGAAACAGCTCAAGCGCGCGGGCATCAACTTAACCATGGACGTGGTCGAGCACGCC
GCTTATCACGCGCAGATCCGCAAGGACGTGAGTGCAATCGTCTTCTACGGCGCTGCTCGATTCCCGGTGGCCGATTCCTA
CCTCACAGAGTTCTATCACTCCCGCTCCGAGATCGGCGCACCTACTCAGGTTACCAATTTCTCCCACTGCAATGCAGCCG
ACAAGGAAATTGATGCGGCGCGCGCAACCCCAAATGCGGCTGCGCGAAGCTCGCTTTGGCGCGTGGCACAGGTGAAGATC
AACGCAGATCTCTGCGCCATCCCGCTATTTGATCTGCAGCAGGTCTGGGCCCGTCGCGGTGCGCTCGACTATGGAGTGCC
TCTTGAGGGCGCAATGAACCTCTTTCCGCCGATCAACGAAAAATCGACACTGAAGAAATGA

Upstream 100 bases:

>100_bases
GACGTCCACCCGTGCCACTGCCCAGTGCGAGATGGGAGCACCCGACGTTCTTATAGAGATGATCGTCACTGCGGCCATCG
CAAAAAAGGAAGTAGCATAA

Downstream 100 bases:

>100_bases
TCGCTACGAGCAGTACCTGAGTGGTGAACTGCGATCTCGCCGTAATCGGTTGCGGGGTCGACGGCTTCAGTTGCGTACTA
CTCGGCCGCGTGATCAGTGG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MKAVDYVIACRALLIAACIGLVGPVFAQESILRIGSDAIDITTLDPHRTAVSEEKGLISMIFGGLVRFPPGSADPEKIEG
DLAESWQASADGLTWTFKLRHGVQFHRNYGEVNAEDVVYSLMRAADPKRSSFSSTFEPVKEVSALDSHTVRIQLKAPVPS
LLGLVANYHGGMVVSRKADQDLKDAFKLKPVGFGPFEFVEHQKQREVVLKAHDKYFRGKPKIERIVYRFIPSEAVRGLAF
ATGELDLVAGQRDQRWVQRARLWAPPKDQSSVKVDVFGPGEFRTLMLNRRIKPLDDPRVREAVARAVDVQELVHFVGADI
VKPGRSVIPPGYAGEVDVGPKFPYNVGKSKALLTEAGYANGITLRAVVSSTASQLSVMDVVQKQLKRAGINLTMDVVEHA
AYHAQIRKDVSAIVFYGAARFPVADSYLTEFYHSRSEIGAPTQVTNFSHCNAADKEIDAARATPNAAARSSLWRVAQVKI
NADLCAIPLFDLQQVWARRGALDYGVPLEGAMNLFPPINEKSTLKK

Sequences:

>Translated_526_residues
MKAVDYVIACRALLIAACIGLVGPVFAQESILRIGSDAIDITTLDPHRTAVSEEKGLISMIFGGLVRFPPGSADPEKIEG
DLAESWQASADGLTWTFKLRHGVQFHRNYGEVNAEDVVYSLMRAADPKRSSFSSTFEPVKEVSALDSHTVRIQLKAPVPS
LLGLVANYHGGMVVSRKADQDLKDAFKLKPVGFGPFEFVEHQKQREVVLKAHDKYFRGKPKIERIVYRFIPSEAVRGLAF
ATGELDLVAGQRDQRWVQRARLWAPPKDQSSVKVDVFGPGEFRTLMLNRRIKPLDDPRVREAVARAVDVQELVHFVGADI
VKPGRSVIPPGYAGEVDVGPKFPYNVGKSKALLTEAGYANGITLRAVVSSTASQLSVMDVVQKQLKRAGINLTMDVVEHA
AYHAQIRKDVSAIVFYGAARFPVADSYLTEFYHSRSEIGAPTQVTNFSHCNAADKEIDAARATPNAAARSSLWRVAQVKI
NADLCAIPLFDLQQVWARRGALDYGVPLEGAMNLFPPINEKSTLKK
>Mature_526_residues
MKAVDYVIACRALLIAACIGLVGPVFAQESILRIGSDAIDITTLDPHRTAVSEEKGLISMIFGGLVRFPPGSADPEKIEG
DLAESWQASADGLTWTFKLRHGVQFHRNYGEVNAEDVVYSLMRAADPKRSSFSSTFEPVKEVSALDSHTVRIQLKAPVPS
LLGLVANYHGGMVVSRKADQDLKDAFKLKPVGFGPFEFVEHQKQREVVLKAHDKYFRGKPKIERIVYRFIPSEAVRGLAF
ATGELDLVAGQRDQRWVQRARLWAPPKDQSSVKVDVFGPGEFRTLMLNRRIKPLDDPRVREAVARAVDVQELVHFVGADI
VKPGRSVIPPGYAGEVDVGPKFPYNVGKSKALLTEAGYANGITLRAVVSSTASQLSVMDVVQKQLKRAGINLTMDVVEHA
AYHAQIRKDVSAIVFYGAARFPVADSYLTEFYHSRSEIGAPTQVTNFSHCNAADKEIDAARATPNAAARSSLWRVAQVKI
NADLCAIPLFDLQQVWARRGALDYGVPLEGAMNLFPPINEKSTLKK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=499, Percent_Identity=27.8557114228457, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1789966, Length=454, Percent_Identity=27.3127753303965, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1787551, Length=442, Percent_Identity=25.1131221719457, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787762, Length=494, Percent_Identity=25.1012145748988, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789887, Length=479, Percent_Identity=25.0521920668058, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787495, Length=380, Percent_Identity=25, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI87081878, Length=435, Percent_Identity=23.2183908045977, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57845; Mature: 57845

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVDYVIACRALLIAACIGLVGPVFAQESILRIGSDAIDITTLDPHRTAVSEEKGLISM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCHHHH
IFGGLVRFPPGSADPEKIEGDLAESWQASADGLTWTFKLRHGVQFHRNYGEVNAEDVVYS
HHCCHHCCCCCCCCHHHHCCHHHHHHCCCCCCEEEEEEECCCHHHHHCCCCCCHHHHHHH
LMRAADPKRSSFSSTFEPVKEVSALDSHTVRIQLKAPVPSLLGLVANYHGGMVVSRKADQ
HHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCH
DLKDAFKLKPVGFGPFEFVEHQKQREVVLKAHDKYFRGKPKIERIVYRFIPSEAVRGLAF
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHEE
ATGELDLVAGQRDQRWVQRARLWAPPKDQSSVKVDVFGPGEFRTLMLNRRIKPLDDPRVR
ECCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCCCCHHHH
EAVARAVDVQELVHFVGADIVKPGRSVIPPGYAGEVDVGPKFPYNVGKSKALLTEAGYAN
HHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCC
GITLRAVVSSTASQLSVMDVVQKQLKRAGINLTMDVVEHAAYHAQIRKDVSAIVFYGAAR
CEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHEEEECCCC
FPVADSYLTEFYHSRSEIGAPTQVTNFSHCNAADKEIDAARATPNAAARSSLWRVAQVKI
CCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHEEEEEEE
NADLCAIPLFDLQQVWARRGALDYGVPLEGAMNLFPPINEKSTLKK
CCCEEECCHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCHHCCCC
>Mature Secondary Structure
MKAVDYVIACRALLIAACIGLVGPVFAQESILRIGSDAIDITTLDPHRTAVSEEKGLISM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCHHHH
IFGGLVRFPPGSADPEKIEGDLAESWQASADGLTWTFKLRHGVQFHRNYGEVNAEDVVYS
HHCCHHCCCCCCCCHHHHCCHHHHHHCCCCCCEEEEEEECCCHHHHHCCCCCCHHHHHHH
LMRAADPKRSSFSSTFEPVKEVSALDSHTVRIQLKAPVPSLLGLVANYHGGMVVSRKADQ
HHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCH
DLKDAFKLKPVGFGPFEFVEHQKQREVVLKAHDKYFRGKPKIERIVYRFIPSEAVRGLAF
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHEE
ATGELDLVAGQRDQRWVQRARLWAPPKDQSSVKVDVFGPGEFRTLMLNRRIKPLDDPRVR
ECCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCCCCHHHH
EAVARAVDVQELVHFVGADIVKPGRSVIPPGYAGEVDVGPKFPYNVGKSKALLTEAGYAN
HHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCC
GITLRAVVSSTASQLSVMDVVQKQLKRAGINLTMDVVEHAAYHAQIRKDVSAIVFYGAAR
CEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHEEEECCCC
FPVADSYLTEFYHSRSEIGAPTQVTNFSHCNAADKEIDAARATPNAAARSSLWRVAQVKI
CCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHEEEEEEE
NADLCAIPLFDLQQVWARRGALDYGVPLEGAMNLFPPINEKSTLKK
CCCEEECCHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA