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The map label for this gene is sspA [H]

Identifier: 91790014

GI number: 91790014

Start: 4391792

End: 4392469

Strand: Reverse

Name: sspA [H]

Synonym: Bpro_4176

Alternate gene names: 91790014

Gene position: 4392469-4391792 (Counterclockwise)

Preceding gene: 91790015

Following gene: 91790013

Centisome position: 84.47

GC content: 62.98

Gene sequence:

>678_bases
ATGGCCTCCCAACTCACATTGATCAGCCACAAGCTGTGCCCTTATGTCCAGCGCGCGGCGATTGTCATGGCCGAGAAGTG
CATTAGTTTTGAGCGGCAGGACATTGACCTGGCCCACAAGCCCGACTGGTTCCTCAAGGTCTCGCCGCTGGGCAAGACGC
CCGTGCTGCGGGTGGACGGCGAAGCGATTTTTGAATCCGCCGTCATCTGCGAATACCTCGACGAAACAGCCGCACCCCGC
CTGCACCCCGAGGGCGCCCTGCAACGTGCCCGGCACCGCAGCTGGATGGAGTTCGGTTCTGCGATCCTCAACACCATCGG
CGCCTTCTATAACGCGCCCAGTGAGGAAGCCCTGGCGGCCCGCGCCGCCGAGCTGCGCGCAAAGTTCCAGCTGGTCGAAG
CGGCCCTGGGAAGCGGCCCCTACTTCGCTGGCGAACAGTTCAGCATGGTGGATGCGGTCTTCGGCCCGGTCTTCCGGTAC
TTCGACGTGTTCGACGAAATCGGTGATTTCGGTGTTTTCGCCGATACGCCCAAGGTGAAGGCCTGGCGGCGCGCGCTGGC
ACAACGTGAATCGGTCCGCAACGCGGTTCGTCCTGATTACCCTGAATTTCTGCGCGCCTTTCTGCGGCAACGCGAGTCGG
CGCTGTCCCGGCGCATAACTGTCGCGCTGCCAGCTTGA

Upstream 100 bases:

>100_bases
CTTCCTGACAGTGGCCTCCGTGGCGCTTTGGCTCGCCGGCGACGGCGCCTTCGCCCTGCGCCGCAGCCTGCGTTTTGTGC
CGGCCTTGTGAAGGAGCACC

Downstream 100 bases:

>100_bases
GAGCCGGACATTTGCAAGGGAGCGAACTCTTTTTTTCAAGGTTTGCGGATCGGGTGTCGAAATGCAGGTTTGTCGTTCGT
CGTAGGGGTGTAAGGTTCAA

Product: glutathione S-transferase-like protein

Products: HX; R-S-glutathione

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MASQLTLISHKLCPYVQRAAIVMAEKCISFERQDIDLAHKPDWFLKVSPLGKTPVLRVDGEAIFESAVICEYLDETAAPR
LHPEGALQRARHRSWMEFGSAILNTIGAFYNAPSEEALAARAAELRAKFQLVEAALGSGPYFAGEQFSMVDAVFGPVFRY
FDVFDEIGDFGVFADTPKVKAWRRALAQRESVRNAVRPDYPEFLRAFLRQRESALSRRITVALPA

Sequences:

>Translated_225_residues
MASQLTLISHKLCPYVQRAAIVMAEKCISFERQDIDLAHKPDWFLKVSPLGKTPVLRVDGEAIFESAVICEYLDETAAPR
LHPEGALQRARHRSWMEFGSAILNTIGAFYNAPSEEALAARAAELRAKFQLVEAALGSGPYFAGEQFSMVDAVFGPVFRY
FDVFDEIGDFGVFADTPKVKAWRRALAQRESVRNAVRPDYPEFLRAFLRQRESALSRRITVALPA
>Mature_224_residues
ASQLTLISHKLCPYVQRAAIVMAEKCISFERQDIDLAHKPDWFLKVSPLGKTPVLRVDGEAIFESAVICEYLDETAAPRL
HPEGALQRARHRSWMEFGSAILNTIGAFYNAPSEEALAARAAELRAKFQLVEAALGSGPYFAGEQFSMVDAVFGPVFRYF
DVFDEIGDFGVFADTPKVKAWRRALAQRESVRNAVRPDYPEFLRAFLRQRESALSRRITVALPA

Specific function: Forms an equimolar complex with the RNA polymerase holoenzyme (RNAP) but not with the core enzyme [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI4758484, Length=218, Percent_Identity=30.2752293577982, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI300360534, Length=212, Percent_Identity=30.6603773584906, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI38016131, Length=192, Percent_Identity=30.2083333333333, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI300360532, Length=216, Percent_Identity=26.8518518518519, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI300360569, Length=188, Percent_Identity=30.3191489361702, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI300360567, Length=188, Percent_Identity=27.6595744680851, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1789624, Length=162, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25144449, Length=152, Percent_Identity=31.5789473684211, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI21355763, Length=198, Percent_Identity=31.8181818181818, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24660979, Length=201, Percent_Identity=30.3482587064677, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21355779, Length=220, Percent_Identity=30, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21355775, Length=157, Percent_Identity=33.1210191082803, Blast_Score=74, Evalue=6e-14,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 25226; Mature: 25095

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASQLTLISHKLCPYVQRAAIVMAEKCISFERQDIDLAHKPDWFLKVSPLGKTPVLRVDG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEECH
EAIFESAVICEYLDETAAPRLHPEGALQRARHRSWMEFGSAILNTIGAFYNAPSEEALAA
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
RAAELRAKFQLVEAALGSGPYFAGEQFSMVDAVFGPVFRYFDVFDEIGDFGVFADTPKVK
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
AWRRALAQRESVRNAVRPDYPEFLRAFLRQRESALSRRITVALPA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCEEEEEECC
>Mature Secondary Structure 
ASQLTLISHKLCPYVQRAAIVMAEKCISFERQDIDLAHKPDWFLKVSPLGKTPVLRVDG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEECH
EAIFESAVICEYLDETAAPRLHPEGALQRARHRSWMEFGSAILNTIGAFYNAPSEEALAA
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
RAAELRAKFQLVEAALGSGPYFAGEQFSMVDAVFGPVFRYFDVFDEIGDFGVFADTPKVK
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
AWRRALAQRESVRNAVRPDYPEFLRAFLRQRESALSRRITVALPA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]