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The map label for this gene is lspL [H]

Identifier: 91789853

GI number: 91789853

Start: 4212851

End: 4213723

Strand: Direct

Name: lspL [H]

Synonym: Bpro_4013

Alternate gene names: 91789853

Gene position: 4212851-4213723 (Clockwise)

Preceding gene: 91789852

Following gene: 91789854

Centisome position: 81.01

GC content: 55.21

Gene sequence:

>873_bases
TTGAGAATTTTATTGACAGGTGCCAATGGGTTTACCGGACGGCACTTTACCGCCCTGGCCCAACAAAAAGGGCACGAGGT
AGTGGCCCTTGCGGCAGACTTGACGGACGCCGAAGCGCTGAAGGCGGAAGTTGCCTCTATACAGCCCTCTCATGTGGTAC
ATCTCGCTGCCATTAGCTTCGTAGGCCATGCTGATGTGCGGGCTTTTTATGATGTCAATCTGTTCGGCACGCTCAACCTT
CTGAACGCGATCGCAGCGCTTGAGATCTCACCACGTTCGGTCATTCTTACCAGCAGTGCCAATGTGTATGGTAATTGCGC
TGCATCGCCTATTCCCGAAGACCAAATTCCGGCGCCATTGAACCACTACGGCATCAGTAAACTGGCGATGGAGCATTTGG
CACGCACCTATTCAGACATATTACCCATAGTCATTGTCCGCCCGTTTAATTACACGGGGTCAGGACAGGCGCTCACATTT
GTCATTCCCAAACTGATAGACCACTTCAGCCGCGGCGCACCGCGCATTCAGTTAGGAGACGTCAGCGTAGAGCGAGAGTT
CAACGACATACTGTTTGTTTGCGAGGCTTACCTCCAGCTGCTGGAGCACGGCCAGCCTGGCGAAACGTATAACGTGTGCT
CTGGCCAGCCCTATACATTGCAGCACGTGATTGACACTCTTGTACGTATCACGGGGCACAGCATCGAGGTCGAGGTCGAC
CCCGCCTTTGTGCGCTCCAACGAGGTAAACCGCCTGTGCGGCAGCCCGACCAAGCTAAAGGCGCTGCTGGCCAGGCATGA
CTACACGCTAAACAATCTGCCACTGGAAGACACGCTGCGGCGCATGCTGGCCGAGGCGGATGCTAACGCCTGA

Upstream 100 bases:

>100_bases
CCAAAGCCAAGTTGGGCTGGGAACCAAAGACGACGCTTGAACAGCTGTGCCAGATGATGGTCGAGGCTGACCTGCGGCGC
AACCAGGCGGGGTTTTCGTT

Downstream 100 bases:

>100_bases
ACGGCAATGTTCACCGTGAACCACGCATCGGCCATGCCGAGACTGACTTATGCACGGGCTGGTGCGCAGATTCTGCAGGC
GCATCTGAAGTTGGGCACCG

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: UDP-glucuronic acid epimerase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRILLTGANGFTGRHFTALAQQKGHEVVALAADLTDAEALKAEVASIQPSHVVHLAAISFVGHADVRAFYDVNLFGTLNL
LNAIAALEISPRSVILTSSANVYGNCAASPIPEDQIPAPLNHYGISKLAMEHLARTYSDILPIVIVRPFNYTGSGQALTF
VIPKLIDHFSRGAPRIQLGDVSVEREFNDILFVCEAYLQLLEHGQPGETYNVCSGQPYTLQHVIDTLVRITGHSIEVEVD
PAFVRSNEVNRLCGSPTKLKALLARHDYTLNNLPLEDTLRRMLAEADANA

Sequences:

>Translated_290_residues
MRILLTGANGFTGRHFTALAQQKGHEVVALAADLTDAEALKAEVASIQPSHVVHLAAISFVGHADVRAFYDVNLFGTLNL
LNAIAALEISPRSVILTSSANVYGNCAASPIPEDQIPAPLNHYGISKLAMEHLARTYSDILPIVIVRPFNYTGSGQALTF
VIPKLIDHFSRGAPRIQLGDVSVEREFNDILFVCEAYLQLLEHGQPGETYNVCSGQPYTLQHVIDTLVRITGHSIEVEVD
PAFVRSNEVNRLCGSPTKLKALLARHDYTLNNLPLEDTLRRMLAEADANA
>Mature_290_residues
MRILLTGANGFTGRHFTALAQQKGHEVVALAADLTDAEALKAEVASIQPSHVVHLAAISFVGHADVRAFYDVNLFGTLNL
LNAIAALEISPRSVILTSSANVYGNCAASPIPEDQIPAPLNHYGISKLAMEHLARTYSDILPIVIVRPFNYTGSGQALTF
VIPKLIDHFSRGAPRIQLGDVSVEREFNDILFVCEAYLQLLEHGQPGETYNVCSGQPYTLQHVIDTLVRITGHSIEVEVD
PAFVRSNEVNRLCGSPTKLKALLARHDYTLNNLPLEDTLRRMLAEADANA

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=259, Percent_Identity=27.4131274131274, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1788366, Length=353, Percent_Identity=23.2294617563739, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=260, Percent_Identity=24.2307692307692, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=260, Percent_Identity=24.2307692307692, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=260, Percent_Identity=24.2307692307692, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=260, Percent_Identity=24.2307692307692, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=260, Percent_Identity=24.2307692307692, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71982035, Length=305, Percent_Identity=23.9344262295082, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.12 [H]

Molecular weight: Translated: 31600; Mature: 31600

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILLTGANGFTGRHFTALAQQKGHEVVALAADLTDAEALKAEVASIQPSHVVHLAAISF
CEEEEECCCCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHH
VGHADVRAFYDVNLFGTLNLLNAIAALEISPRSVILTSSANVYGNCAASPIPEDQIPAPL
HCCCCCEEEEEEEHHHHHHHHHHHHHEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCH
NHYGISKLAMEHLARTYSDILPIVIVRPFNYTGSGQALTFVIPKLIDHFSRGAPRIQLGD
HHCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECC
VSVEREFNDILFVCEAYLQLLEHGQPGETYNVCSGQPYTLQHVIDTLVRITGHSIEVEVD
EEEHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEHHHHHHHHHHHCCCEEEEEEC
PAFVRSNEVNRLCGSPTKLKALLARHDYTLNNLPLEDTLRRMLAEADANA
HHHCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRILLTGANGFTGRHFTALAQQKGHEVVALAADLTDAEALKAEVASIQPSHVVHLAAISF
CEEEEECCCCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHH
VGHADVRAFYDVNLFGTLNLLNAIAALEISPRSVILTSSANVYGNCAASPIPEDQIPAPL
HCCCCCEEEEEEEHHHHHHHHHHHHHEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCH
NHYGISKLAMEHLARTYSDILPIVIVRPFNYTGSGQALTFVIPKLIDHFSRGAPRIQLGD
HHCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECC
VSVEREFNDILFVCEAYLQLLEHGQPGETYNVCSGQPYTLQHVIDTLVRITGHSIEVEVD
EEEHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEHHHHHHHHHHHCCCEEEEEEC
PAFVRSNEVNRLCGSPTKLKALLARHDYTLNNLPLEDTLRRMLAEADANA
HHHCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9765575; 11481430 [H]