Click here to switch to the map view.

The map label for this gene is gmd [H]

Identifier: 91789852

GI number: 91789852

Start: 4211781

End: 4212854

Strand: Direct

Name: gmd [H]

Synonym: Bpro_4012

Alternate gene names: 91789852

Gene position: 4211781-4212854 (Clockwise)

Preceding gene: 91789851

Following gene: 91789853

Centisome position: 80.99

GC content: 57.64

Gene sequence:

>1074_bases
ATGAAAAAAGCGATCATCACCGGAATCTCCGGCCAGGACGGCGCCTACCTCACCCAGCTCCTGCTCGACAAGGGCTACAC
CGTCTACGGCACCTACCGCCGAACCAGTTCGGTCAACTTCTGGCGCATTGAAGAGCTGGGCATCAAAGACCACCCCAACC
TTCATCTCGTTGAATACGATTTGACGGACCTTGGCTCCAACATTGCGCTAGTGCAAAAAGTGCAGCCAGACGAGATCTAC
AACCTGGCCGCCCAAAGCTTTGTGGGCGTGAGCTTTGACCAGCCCACCACCACGGCGCAGATCACTGGCATTGGCGCGCT
GAACCTGCTGGAGGCCATTCGCCTGGTCAACCCCAAGATTCGTTTTTACCAGGCCAGCACGTCGGAGATGTTTGGCAAGG
TGCAGGCTATCCCTCAGGTGGAAGACACGCCTTTTTACCCGCGCAGCCCCTATGGCGTGGCCAAGCTTTACGCACATTGG
ATGACGGTGAACTACCGCGAGAGCTACAACATCTTTGGCAGCAGCGGCATTTTGTTCAACCATGAGAGCCCGCTGCGCGG
CCGCGAGTTTGTGACACGCAAGATCACTGACAGTGTGGCCAAAATCAAGCTGGGCAAGCTTGACTGTATGGATCTTGGCA
ACATCGACGCCAAGCGCGACTGGGGCTTTGCCAAAGAATATGTTGAAGGCATGTGGCGCATGCTGCAGGCCGATGAACCC
GACACTTTTGTGCTCGCAACCAACCGCACCGAGACCGTGCGTGACTTTGTGCGTATGGCATTCAAGGGCGCGGGCATTGC
GGTGGAGTTCAAGGGGTCGGCTGAAGCCGAAACTGCGGTGGTGGCGGCCCTCACCCCTGCCCTCGCCCAGGGAGAGAGGG
AGCTAAAAGTTGGGCAAACCGTGATGCGCATCAACCCAAAGTTTTATCGCCCGGCAGAGGTGGAGTTGCTGATAGGCAAC
CCGGCCAAGGCCAAAGCCAAGTTGGGCTGGGAACCAAAGACGACGCTTGAACAGCTGTGCCAGATGATGGTCGAGGCTGA
CCTGCGGCGCAACCAGGCGGGGTTTTCGTTTTGA

Upstream 100 bases:

>100_bases
CATAGCCTTGACTGAAAGCAGGGTTGGCCCAGCCTTTTCTTCGTTCGGAATAGGACTGCGCCATGTCAATATATTCAACC
GACTCAAAGTGAATGAAACC

Downstream 100 bases:

>100_bases
GAATTTTATTGACAGGTGCCAATGGGTTTACCGGACGGCACTTTACCGCCCTGGCCCAACAAAAAGGGCACGAGGTAGTG
GCCCTTGCGGCAGACTTGAC

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKKAIITGISGQDGAYLTQLLLDKGYTVYGTYRRTSSVNFWRIEELGIKDHPNLHLVEYDLTDLGSNIALVQKVQPDEIY
NLAAQSFVGVSFDQPTTTAQITGIGALNLLEAIRLVNPKIRFYQASTSEMFGKVQAIPQVEDTPFYPRSPYGVAKLYAHW
MTVNYRESYNIFGSSGILFNHESPLRGREFVTRKITDSVAKIKLGKLDCMDLGNIDAKRDWGFAKEYVEGMWRMLQADEP
DTFVLATNRTETVRDFVRMAFKGAGIAVEFKGSAEAETAVVAALTPALAQGERELKVGQTVMRINPKFYRPAEVELLIGN
PAKAKAKLGWEPKTTLEQLCQMMVEADLRRNQAGFSF

Sequences:

>Translated_357_residues
MKKAIITGISGQDGAYLTQLLLDKGYTVYGTYRRTSSVNFWRIEELGIKDHPNLHLVEYDLTDLGSNIALVQKVQPDEIY
NLAAQSFVGVSFDQPTTTAQITGIGALNLLEAIRLVNPKIRFYQASTSEMFGKVQAIPQVEDTPFYPRSPYGVAKLYAHW
MTVNYRESYNIFGSSGILFNHESPLRGREFVTRKITDSVAKIKLGKLDCMDLGNIDAKRDWGFAKEYVEGMWRMLQADEP
DTFVLATNRTETVRDFVRMAFKGAGIAVEFKGSAEAETAVVAALTPALAQGERELKVGQTVMRINPKFYRPAEVELLIGN
PAKAKAKLGWEPKTTLEQLCQMMVEADLRRNQAGFSF
>Mature_357_residues
MKKAIITGISGQDGAYLTQLLLDKGYTVYGTYRRTSSVNFWRIEELGIKDHPNLHLVEYDLTDLGSNIALVQKVQPDEIY
NLAAQSFVGVSFDQPTTTAQITGIGALNLLEAIRLVNPKIRFYQASTSEMFGKVQAIPQVEDTPFYPRSPYGVAKLYAHW
MTVNYRESYNIFGSSGILFNHESPLRGREFVTRKITDSVAKIKLGKLDCMDLGNIDAKRDWGFAKEYVEGMWRMLQADEP
DTFVLATNRTETVRDFVRMAFKGAGIAVEFKGSAEAETAVVAALTPALAQGERELKVGQTVMRINPKFYRPAEVELLIGN
PAKAKAKLGWEPKTTLEQLCQMMVEADLRRNQAGFSF

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=354, Percent_Identity=51.1299435028249, Blast_Score=359, Evalue=2e-99,
Organism=Escherichia coli, GI1788366, Length=352, Percent_Identity=51.1363636363636, Blast_Score=367, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17539424, Length=354, Percent_Identity=51.9774011299435, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI133901790, Length=354, Percent_Identity=51.9774011299435, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI133901788, Length=354, Percent_Identity=51.9774011299435, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17539422, Length=354, Percent_Identity=51.9774011299435, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI133901786, Length=354, Percent_Identity=51.9774011299435, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17507723, Length=356, Percent_Identity=51.685393258427, Blast_Score=359, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24158427, Length=359, Percent_Identity=48.7465181058496, Blast_Score=337, Evalue=8e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 39974; Mature: 39974

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAIITGISGQDGAYLTQLLLDKGYTVYGTYRRTSSVNFWRIEELGIKDHPNLHLVEYD
CCCEEEECCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCCCCEEEEEEE
LTDLGSNIALVQKVQPDEIYNLAAQSFVGVSFDQPTTTAQITGIGALNLLEAIRLVNPKI
HHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCE
RFYQASTSEMFGKVQAIPQVEDTPFYPRSPYGVAKLYAHWMTVNYRESYNIFGSSGILFN
EEEECCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCCEEECCCEEEC
HESPLRGREFVTRKITDSVAKIKLGKLDCMDLGNIDAKRDWGFAKEYVEGMWRMLQADEP
CCCCCCHHHHHHHHHHHHHHHEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
DTFVLATNRTETVRDFVRMAFKGAGIAVEFKGSAEAETAVVAALTPALAQGERELKVGQT
CEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHEEHHHHHHHHHCCCHHHHHCCE
VMRINPKFYRPAEVELLIGNPAKAKAKLGWEPKTTLEQLCQMMVEADLRRNQAGFSF
EEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKAIITGISGQDGAYLTQLLLDKGYTVYGTYRRTSSVNFWRIEELGIKDHPNLHLVEYD
CCCEEEECCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCCCCEEEEEEE
LTDLGSNIALVQKVQPDEIYNLAAQSFVGVSFDQPTTTAQITGIGALNLLEAIRLVNPKI
HHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCE
RFYQASTSEMFGKVQAIPQVEDTPFYPRSPYGVAKLYAHWMTVNYRESYNIFGSSGILFN
EEEECCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCCEEECCCEEEC
HESPLRGREFVTRKITDSVAKIKLGKLDCMDLGNIDAKRDWGFAKEYVEGMWRMLQADEP
CCCCCCHHHHHHHHHHHHHHHEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
DTFVLATNRTETVRDFVRMAFKGAGIAVEFKGSAEAETAVVAALTPALAQGERELKVGQT
CEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHEEHHHHHHHHHCCCHHHHHCCE
VMRINPKFYRPAEVELLIGNPAKAKAKLGWEPKTTLEQLCQMMVEADLRRNQAGFSF
EEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]