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The map label for this gene is mdlA [H]

Identifier: 91789802

GI number: 91789802

Start: 4154185

End: 4155297

Strand: Reverse

Name: mdlA [H]

Synonym: Bpro_3962

Alternate gene names: 91789802

Gene position: 4155297-4154185 (Counterclockwise)

Preceding gene: 91789810

Following gene: 91789801

Centisome position: 79.91

GC content: 66.85

Gene sequence:

>1113_bases
ATGAAACCGGAAGTAACGATCGAATCCATCCGCCTGCGGCAGGTCAATTTGCCGCCCAAGGTCGTGCGCACCGATGCCAT
CCAGTCCTTTGTGACGCAGGAGACGCTGCTGCTCACCTTGCGCTGCAGCGATGGCGTGGAGGGCACCGGTTATGCGTACA
CCATTGGCACCGGCGGCTCATCGGTGATGGCCTTGCTGCACGACCACCTGGCGCCGCGCCTGCTGGGCCGCAACCCGCTG
CTGATTGAAGCGATCTGGAAAGACCTGTTTTTCCATACCCATGCCACCGCCGTCGGGGCCATCACCAGCCTGGCGCTGGC
CTGCGTGGACACCGCGCTGTGGGACTGGCGCGCCCGCAGCCAGGGCCTGCCCTTGTGGCAATTGCTGGGTGGCGCGCAGC
CGCGGGTGCCGGTCTACACCACCGAAGGCGGCTGGCTGCACCTGAGCGCGGACGAACTGGTGGACCAGACGCTGGCCGCC
AAGGCCGAGGGTTTCAAGGGCGCCAAGATCAAGGTCGGCCGGCCGCATGTCAGCGAGGACGTGGCGCGCCTGTCCAAGGT
GCGCGAGGCGGTCGGTGCGGGGTTCGACATCATGACCGATGCGAACCAGGGCTTCAACCGCCCCGAAGCCGTGCGCCGGG
CGGCGGCTTTTGCCGCGCTGGACCTCGCCTGGCTGGAAGAGCCGCTGCCCGCGGAAAACATCTCCGGCCACCGGCATTTG
CGTGAACACACGGCCATTCCGGTGGCGGTGGGCGAGTCGATGTACCACCTCAGCCAGTTCCGCGAATACCTGGAGCAGGG
CGCCTGCAGCATCGTCCAGCCCGACGTGGCCCGCATTGGCGGCATCACGCCCTGGATCAAGGTGGCGCATCTGGCCGAGG
GCTTTGATGTGCCGGTGTGTCCGCACTTCCTGATGGAGCTGCATGTGAGCCTGTGCGCCGCAGTGCCCAACGCGGCCTGG
GTCGAGTACATACCGCAGCTCGACGACATCACGACCAGCCGCATCCGCGTCGACGGCGGCTACGCCTTCCCGCCGGACAC
GCCCGGCCTGGGCATCGAATGGGACTGGCCCGTCATCGGCAAGCGGCAGCTTGCCGACGTGACCATCAAGTAA

Upstream 100 bases:

>100_bases
ATCCACCGGCGGTGGCATCCGGCTAAACCCTGTAATACCGTTCATGACCTGAATAACCGACCTGAATAACCGCGAATAAC
CCTGGATACCCTTCTGCACC

Downstream 100 bases:

>100_bases
GTCATCGCAATCATCACAACCATCGTCAACAACCAAGGAAAAAGCATGAGACTCCAGGGAAAAACCGCACTGGTCACGGC
CGCCGGACAGGGCATCGGAA

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: MR [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKPEVTIESIRLRQVNLPPKVVRTDAIQSFVTQETLLLTLRCSDGVEGTGYAYTIGTGGSSVMALLHDHLAPRLLGRNPL
LIEAIWKDLFFHTHATAVGAITSLALACVDTALWDWRARSQGLPLWQLLGGAQPRVPVYTTEGGWLHLSADELVDQTLAA
KAEGFKGAKIKVGRPHVSEDVARLSKVREAVGAGFDIMTDANQGFNRPEAVRRAAAFAALDLAWLEEPLPAENISGHRHL
REHTAIPVAVGESMYHLSQFREYLEQGACSIVQPDVARIGGITPWIKVAHLAEGFDVPVCPHFLMELHVSLCAAVPNAAW
VEYIPQLDDITTSRIRVDGGYAFPPDTPGLGIEWDWPVIGKRQLADVTIK

Sequences:

>Translated_370_residues
MKPEVTIESIRLRQVNLPPKVVRTDAIQSFVTQETLLLTLRCSDGVEGTGYAYTIGTGGSSVMALLHDHLAPRLLGRNPL
LIEAIWKDLFFHTHATAVGAITSLALACVDTALWDWRARSQGLPLWQLLGGAQPRVPVYTTEGGWLHLSADELVDQTLAA
KAEGFKGAKIKVGRPHVSEDVARLSKVREAVGAGFDIMTDANQGFNRPEAVRRAAAFAALDLAWLEEPLPAENISGHRHL
REHTAIPVAVGESMYHLSQFREYLEQGACSIVQPDVARIGGITPWIKVAHLAEGFDVPVCPHFLMELHVSLCAAVPNAAW
VEYIPQLDDITTSRIRVDGGYAFPPDTPGLGIEWDWPVIGKRQLADVTIK
>Mature_370_residues
MKPEVTIESIRLRQVNLPPKVVRTDAIQSFVTQETLLLTLRCSDGVEGTGYAYTIGTGGSSVMALLHDHLAPRLLGRNPL
LIEAIWKDLFFHTHATAVGAITSLALACVDTALWDWRARSQGLPLWQLLGGAQPRVPVYTTEGGWLHLSADELVDQTLAA
KAEGFKGAKIKVGRPHVSEDVARLSKVREAVGAGFDIMTDANQGFNRPEAVRRAAAFAALDLAWLEEPLPAENISGHRHL
REHTAIPVAVGESMYHLSQFREYLEQGACSIVQPDVARIGGITPWIKVAHLAEGFDVPVCPHFLMELHVSLCAAVPNAAW
VEYIPQLDDITTSRIRVDGGYAFPPDTPGLGIEWDWPVIGKRQLADVTIK

Specific function: Probably Involved In The Degradation Of Homoserine Lactone (Hsl) Or Of A Metabolite Of Hsl That Signals Starvation. [C]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=390, Percent_Identity=26.1538461538462, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI42544117, Length=386, Percent_Identity=25.6476683937824, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI186972148, Length=323, Percent_Identity=26.9349845201238, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI1787864, Length=387, Percent_Identity=28.4237726098191, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI48994953, Length=344, Percent_Identity=27.6162790697674, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI226510960, Length=350, Percent_Identity=26, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.1.2.2 [H]

Molecular weight: Translated: 40459; Mature: 40459

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPEVTIESIRLRQVNLPPKVVRTDAIQSFVTQETLLLTLRCSDGVEGTGYAYTIGTGGS
CCCCCCHHHEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEECCCCH
SVMALLHDHLAPRLLGRNPLLIEAIWKDLFFHTHATAVGAITSLALACVDTALWDWRARS
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QGLPLWQLLGGAQPRVPVYTTEGGWLHLSADELVDQTLAAKAEGFKGAKIKVGRPHVSED
CCCCHHHHHCCCCCCCCEEECCCCEEEECHHHHHHHHHHHHHCCCCCCEEEECCCCHHHH
VARLSKVREAVGAGFDIMTDANQGFNRPEAVRRAAAFAALDLAWLEEPLPAENISGHRHL
HHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
REHTAIPVAVGESMYHLSQFREYLEQGACSIVQPDVARIGGITPWIKVAHLAEGFDVPVC
HHHCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCH
PHFLMELHVSLCAAVPNAAWVEYIPQLDDITTSRIRVDGGYAFPPDTPGLGIEWDWPVIG
HHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEECCCEECCCCCCCCCEEECCCCCC
KRQLADVTIK
CCCCCCEECC
>Mature Secondary Structure
MKPEVTIESIRLRQVNLPPKVVRTDAIQSFVTQETLLLTLRCSDGVEGTGYAYTIGTGGS
CCCCCCHHHEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEECCCCH
SVMALLHDHLAPRLLGRNPLLIEAIWKDLFFHTHATAVGAITSLALACVDTALWDWRARS
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QGLPLWQLLGGAQPRVPVYTTEGGWLHLSADELVDQTLAAKAEGFKGAKIKVGRPHVSED
CCCCHHHHHCCCCCCCCEEECCCCEEEECHHHHHHHHHHHHHCCCCCCEEEECCCCHHHH
VARLSKVREAVGAGFDIMTDANQGFNRPEAVRRAAAFAALDLAWLEEPLPAENISGHRHL
HHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
REHTAIPVAVGESMYHLSQFREYLEQGACSIVQPDVARIGGITPWIKVAHLAEGFDVPVC
HHHCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCH
PHFLMELHVSLCAAVPNAAWVEYIPQLDDITTSRIRVDGGYAFPPDTPGLGIEWDWPVIG
HHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEECCCEECCCCCCCCCEEECCCCCC
KRQLADVTIK
CCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2831968; 2271624; 2215699; 1909893; 1892834; 7893689; 7893690 [H]