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The map label for this gene is speE [H]

Identifier: 91789725

GI number: 91789725

Start: 4076519

End: 4077286

Strand: Reverse

Name: speE [H]

Synonym: Bpro_3883

Alternate gene names: 91789725

Gene position: 4077286-4076519 (Counterclockwise)

Preceding gene: 91789726

Following gene: 91789724

Centisome position: 78.41

GC content: 60.16

Gene sequence:

>768_bases
ATGGCGAAAAAAATTACCGAGAACACCACCCTGGAGTTGCCAGAGGTCAATTTCTCCGACTATGGCGATGTGCGTTTTCT
GCATTTGGGTACTGAATGGGTGCAGGGCTCCATGCTGCGCGATGCGCCTTATGACATTGAGCTGGAGTATGTGCAGCGCA
TGATGGTGGGGCTGCTGTTCATGGACGCTACGGCCGTGTCAAAAAAGCATGCGATGCAGCTTGGGCTGGGCTCGGCGGCG
CTGACCAAGTTCTGCTACAAAAAGCTGCGCATGAAAACCACGGCCATCGAGATCAACCCGCAGGTGATCGCCGCTTGCCG
GCTCTGGTTCAAATTGCCGCGCGACGACCTCAGGCTGCATGTCATCGAGGCCGATGCCGCCAAAGAAATCCAGAACCCGC
GGCATCAGGGGAGTGTTGATCTCCTGCATGTCGACCTGTACGACCACGAAGCGGCGGCGCCGGTGCTGGACAGTGCTGAT
TTTTACGCTGATTGCCGGCGCCTTCTCAGTGATGAGGGGGTGATGACGGTGAACCTCTTCGGCCGGGATTCGAGTTATGT
GCACTCGCTGACCAAGATCGCCGAGGCCTTTGGCCCGGAGGCGGTCTGGGCCTTCCGGCCGACGCGTGAGGGCAACACGG
TCGTGATGGCCCAGCGCGAGCCCACGCGCCCGGACCGGGCCGAACTCCTGCTGCAGGCGGGCGTCATTGAATCACGTTGG
GACCTGCCCGCCAAAAAGTGGCTGCGGCTTTTCAAGCCGGTGACCTGA

Upstream 100 bases:

>100_bases
TTTACCGGTTACCTTCCACCAGCCCGGCCAATGCGTCCTGGTCTTTTGAGCGCAAACTTAGCTCCTGGCGCGAAGTTTTT
GAAAAACACGAATTGATCTG

Downstream 100 bases:

>100_bases
TGCCGGATGAGGACGGGGTGGCAATCGCCGGAGTGGATCGGGTACAGTGCCATCCATGAGCGCCGTGATACCCCCCCCAC
GCCCCACTTCCTCGCGGGAC

Product: hypothetical protein

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MAKKITENTTLELPEVNFSDYGDVRFLHLGTEWVQGSMLRDAPYDIELEYVQRMMVGLLFMDATAVSKKHAMQLGLGSAA
LTKFCYKKLRMKTTAIEINPQVIAACRLWFKLPRDDLRLHVIEADAAKEIQNPRHQGSVDLLHVDLYDHEAAAPVLDSAD
FYADCRRLLSDEGVMTVNLFGRDSSYVHSLTKIAEAFGPEAVWAFRPTREGNTVVMAQREPTRPDRAELLLQAGVIESRW
DLPAKKWLRLFKPVT

Sequences:

>Translated_255_residues
MAKKITENTTLELPEVNFSDYGDVRFLHLGTEWVQGSMLRDAPYDIELEYVQRMMVGLLFMDATAVSKKHAMQLGLGSAA
LTKFCYKKLRMKTTAIEINPQVIAACRLWFKLPRDDLRLHVIEADAAKEIQNPRHQGSVDLLHVDLYDHEAAAPVLDSAD
FYADCRRLLSDEGVMTVNLFGRDSSYVHSLTKIAEAFGPEAVWAFRPTREGNTVVMAQREPTRPDRAELLLQAGVIESRW
DLPAKKWLRLFKPVT
>Mature_254_residues
AKKITENTTLELPEVNFSDYGDVRFLHLGTEWVQGSMLRDAPYDIELEYVQRMMVGLLFMDATAVSKKHAMQLGLGSAAL
TKFCYKKLRMKTTAIEINPQVIAACRLWFKLPRDDLRLHVIEADAAKEIQNPRHQGSVDLLHVDLYDHEAAAPVLDSADF
YADCRRLLSDEGVMTVNLFGRDSSYVHSLTKIAEAFGPEAVWAFRPTREGNTVVMAQREPTRPDRAELLLQAGVIESRWD
LPAKKWLRLFKPVT

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 28964; Mature: 28833

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKITENTTLELPEVNFSDYGDVRFLHLGTEWVQGSMLRDAPYDIELEYVQRMMVGLLF
CCCCCCCCCEEECCCCCCCCCCCEEEEEECHHHHCCCCCCCCCCCEEHHHHHHHHHHHHH
MDATAVSKKHAMQLGLGSAALTKFCYKKLRMKTTAIEINPQVIAACRLWFKLPRDDLRLH
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHCCCCCEEEE
VIEADAAKEIQNPRHQGSVDLLHVDLYDHEAAAPVLDSADFYADCRRLLSDEGVMTVNLF
EEECCHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEE
GRDSSYVHSLTKIAEAFGPEAVWAFRPTREGNTVVMAQREPTRPDRAELLLQAGVIESRW
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCC
DLPAKKWLRLFKPVT
CCCHHHHHHHHCCCC
>Mature Secondary Structure 
AKKITENTTLELPEVNFSDYGDVRFLHLGTEWVQGSMLRDAPYDIELEYVQRMMVGLLF
CCCCCCCCEEECCCCCCCCCCCEEEEEECHHHHCCCCCCCCCCCEEHHHHHHHHHHHHH
MDATAVSKKHAMQLGLGSAALTKFCYKKLRMKTTAIEINPQVIAACRLWFKLPRDDLRLH
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHCCCCCEEEE
VIEADAAKEIQNPRHQGSVDLLHVDLYDHEAAAPVLDSADFYADCRRLLSDEGVMTVNLF
EEECCHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEE
GRDSSYVHSLTKIAEAFGPEAVWAFRPTREGNTVVMAQREPTRPDRAELLLQAGVIESRW
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCC
DLPAKKWLRLFKPVT
CCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]