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The map label for this gene is ywbI [H]

Identifier: 91789656

GI number: 91789656

Start: 4001179

End: 4002144

Strand: Reverse

Name: ywbI [H]

Synonym: Bpro_3812

Alternate gene names: 91789656

Gene position: 4002144-4001179 (Counterclockwise)

Preceding gene: 91789666

Following gene: 91789650

Centisome position: 76.96

GC content: 64.6

Gene sequence:

>966_bases
ATGGATGTCCGCTACCTGCAAAGCTTCGTCACCGTCGTGGAAGTCGGCTCGCTGGCGGAAGCCGCCAGAAGACTGGACTT
GACGCCCGCCGCCATTGCGGCGCGTGTTCATGCACTGGAGGAGGAACTGGGTGTGGCGCTCATCAAGCGCGCCGGGCGAT
CCGTCAAGCCGACCGAGGCGGGCGTGAAAATCCTGGAGCCTTCCCGCTCCGTGCTGCGTGACATACGCGATCTGCGCGTG
GTGGCGGGAGACGGGATTGCGTTGGGCGAATTGCGGCTGGGCGTGTTCGCGTCGGCCATGACCAGCGTGCTTCCGCCGGT
GCTCAAGCGACTCTATGCCAGGTACCCGGACCTCAATGTCTTCGTGGCCACGGGGTCCTCGGTGGAGTTGTGCCGGCAGG
TCAGCACCGGTGCCATTGATGCCGCGATTGTTGTGGAGCCTCAATTTGCCGTGGCCAAAAGTTGCGCATGGCGCTCGCTT
GTGGCGGAACCCCTGGTGGTCGTGGCGCCCGCTGAACTGGCCGGTCATGAGGCCCACGAACTGTTGCGATCAGAGCCATT
CATCCGCTATGACCGGTCGGTCCTCGGAGGGCAACTGGCCGATCGGTATTTGCGCGACCAAAGTATCCGGCCCAGGCAGC
GTCTTGAGATTGACGGCCTCATGGCGATTGCCGCCCTGGTGGCGCAAGGCCTCGGTGTGGCCCTGTTGCCGGACTGGATA
CCGCTGTGGCAATCGGGTCTGGCCATCACCCGCATTCCCTTGCCGGAGCGCCCACCGGTCCGGCACGTCGGGCTGATCTG
GGGCGAGTATGGTCCACGCGCCTCATTGGCTCGCGCATTTCTGGCCGAGGCTGAAGCAGTGTTTGCCAGGCCGGTCCCAC
AGCAGGCCCCCACCCAGACCATCAAGCAGACCATCAAGCCGGCAGCAAGCAGCCGCCGGAAGAAACAAATCGCTGACGCG
GGCTAG

Upstream 100 bases:

>100_bases
CAGAGTTTCCTTGCACACACTGTCAAATAAAACAGGCTTTTCATGTGTTCATACGATAAAATTTTTAACTTTCCGAGCAG
CGTTTCCCCAAGGTGAAATC

Downstream 100 bases:

>100_bases
CCGATGCGGGGCGTGGGCGACAGCGCTGGCTGCCCGTTCAGGACGGCATGGAGGTTGTCCAGCGTCAGACGCAACATGGC
CGTCCGTGCTTCCCGGGTGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MDVRYLQSFVTVVEVGSLAEAARRLDLTPAAIAARVHALEEELGVALIKRAGRSVKPTEAGVKILEPSRSVLRDIRDLRV
VAGDGIALGELRLGVFASAMTSVLPPVLKRLYARYPDLNVFVATGSSVELCRQVSTGAIDAAIVVEPQFAVAKSCAWRSL
VAEPLVVVAPAELAGHEAHELLRSEPFIRYDRSVLGGQLADRYLRDQSIRPRQRLEIDGLMAIAALVAQGLGVALLPDWI
PLWQSGLAITRIPLPERPPVRHVGLIWGEYGPRASLARAFLAEAEAVFARPVPQQAPTQTIKQTIKPAASSRRKKQIADA
G

Sequences:

>Translated_321_residues
MDVRYLQSFVTVVEVGSLAEAARRLDLTPAAIAARVHALEEELGVALIKRAGRSVKPTEAGVKILEPSRSVLRDIRDLRV
VAGDGIALGELRLGVFASAMTSVLPPVLKRLYARYPDLNVFVATGSSVELCRQVSTGAIDAAIVVEPQFAVAKSCAWRSL
VAEPLVVVAPAELAGHEAHELLRSEPFIRYDRSVLGGQLADRYLRDQSIRPRQRLEIDGLMAIAALVAQGLGVALLPDWI
PLWQSGLAITRIPLPERPPVRHVGLIWGEYGPRASLARAFLAEAEAVFARPVPQQAPTQTIKQTIKPAASSRRKKQIADA
G
>Mature_321_residues
MDVRYLQSFVTVVEVGSLAEAARRLDLTPAAIAARVHALEEELGVALIKRAGRSVKPTEAGVKILEPSRSVLRDIRDLRV
VAGDGIALGELRLGVFASAMTSVLPPVLKRLYARYPDLNVFVATGSSVELCRQVSTGAIDAAIVVEPQFAVAKSCAWRSL
VAEPLVVVAPAELAGHEAHELLRSEPFIRYDRSVLGGQLADRYLRDQSIRPRQRLEIDGLMAIAALVAQGLGVALLPDWI
PLWQSGLAITRIPLPERPPVRHVGLIWGEYGPRASLARAFLAEAEAVFARPVPQQAPTQTIKQTIKPAASSRRKKQIADA
G

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=292, Percent_Identity=28.4246575342466, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1790208, Length=246, Percent_Identity=29.2682926829268, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788297, Length=250, Percent_Identity=28.4, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1790399, Length=309, Percent_Identity=26.537216828479, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1788887, Length=286, Percent_Identity=25.1748251748252, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1789639, Length=125, Percent_Identity=32.8, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787806, Length=240, Percent_Identity=26.6666666666667, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI157672245, Length=298, Percent_Identity=25.1677852348993, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI145693105, Length=249, Percent_Identity=24.0963855421687, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787601, Length=268, Percent_Identity=23.8805970149254, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082132, Length=250, Percent_Identity=25.6, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787530, Length=249, Percent_Identity=27.3092369477912, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI2367136, Length=250, Percent_Identity=26.4, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788748, Length=269, Percent_Identity=24.1635687732342, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34724; Mature: 34724

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVRYLQSFVTVVEVGSLAEAARRLDLTPAAIAARVHALEEELGVALIKRAGRSVKPTEA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
GVKILEPSRSVLRDIRDLRVVAGDGIALGELRLGVFASAMTSVLPPVLKRLYARYPDLNV
CCEEECCCHHHHHHHHHHEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
FVATGSSVELCRQVSTGAIDAAIVVEPQFAVAKSCAWRSLVAEPLVVVAPAELAGHEAHE
EEECCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCEEEEECHHHHCHHHHH
LLRSEPFIRYDRSVLGGQLADRYLRDQSIRPRQRLEIDGLMAIAALVAQGLGVALLPDWI
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCHH
PLWQSGLAITRIPLPERPPVRHVGLIWGEYGPRASLARAFLAEAEAVFARPVPQQAPTQT
HHHHCCCEEEECCCCCCCCCCEEHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHH
IKQTIKPAASSRRKKQIADAG
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDVRYLQSFVTVVEVGSLAEAARRLDLTPAAIAARVHALEEELGVALIKRAGRSVKPTEA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
GVKILEPSRSVLRDIRDLRVVAGDGIALGELRLGVFASAMTSVLPPVLKRLYARYPDLNV
CCEEECCCHHHHHHHHHHEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
FVATGSSVELCRQVSTGAIDAAIVVEPQFAVAKSCAWRSLVAEPLVVVAPAELAGHEAHE
EEECCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCEEEEECHHHHCHHHHH
LLRSEPFIRYDRSVLGGQLADRYLRDQSIRPRQRLEIDGLMAIAALVAQGLGVALLPDWI
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCHH
PLWQSGLAITRIPLPERPPVRHVGLIWGEYGPRASLARAFLAEAEAVFARPVPQQAPTQT
HHHHCCCEEEECCCCCCCCCCEEHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHH
IKQTIKPAASSRRKKQIADAG
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]