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The map label for this gene is braE [H]

Identifier: 91789639

GI number: 91789639

Start: 3980864

End: 3981949

Strand: Reverse

Name: braE [H]

Synonym: Bpro_3795

Alternate gene names: 91789639

Gene position: 3981949-3980864 (Counterclockwise)

Preceding gene: 91789640

Following gene: 91789638

Centisome position: 76.57

GC content: 60.41

Gene sequence:

>1086_bases
ATGAAACCCAACAAGCTGGTTACTTTTTTTATAGTTGCCGCCGCGCTGCTGGTGTTGCCCCTGCTGTTGCAGCAGGGCGG
TAACGCCTGGGTGCGCATTGTCGACACGGCGCTGCTCTATGTGCTGCTGGCCCTGGGCCTGAACATTGTGGTGGGCTATG
CCGGCCTGCTGGATCTCGGCTATGTGGCATTTTTTGCGCTCGGTGCCTATCTGTTTGCGCTGCTCGGTTCGCCGCATCTG
GCGGAGGCGTTTCCTGTGATTGCCGCAGCCTTCCCGGAAGGCTTGCACCTGCCCATCTGGGCCGCCATCCCGGCGGCTGC
CGCCCTGGCAGGGTTTTTCGGGGTGGTGCTGGGCGCGCCCACGCTCAAGCTGCGTGGTGACTACCTGGCCATCGTGACGT
TGGGCTTTGGTGAAATCATCCGCGTGTTCCTGAACAACCTGGACCGCCCCATCAACCTGACCAATGGCCCCAAAGGCATC
AACCAGATTGATTCGATGAAGTTTTTCGGCTTCGACCTGGGCAAGCCGCATGAATTTCTCGGCATCGAGGTCAACTCGGT
GTCGATGTACTACTACCTCTTTCTGGTGCTGGTGATCGTCTCGGTCATCATTTGCCACCGGCTGGAAAATTCGCGCATTG
GCCGCGCCTGGATGGCGATTCGCGAAGACGAAATTGCCGCCAAGGCCATGGGCATCAACACCCGCAACATGAAACTGCTG
GCCTTCGGCATGGGTGCTACCTTTGGTGGTGTGTCGGGTTCCATGTTTGCCGCCTTCCAGGGCTTTGTCTCGCCCGAGTC
CTTCAGCCTGATGGAGTCGATCATGATCGTCGCCATGGTGGTGCTGGGCGGCATCGGCCATTTGCCTGGCGTGATTCTGG
GTGCCTTGCTGCTGGCCGCCTTGCCCGAGGTGCTGCGCCATGTGGCCGGTCCGCTGCAGGCCATGACCGATGGCCGGCTG
GACTCTGCCATCCTGCGCCAGTTGCTGATTGCGCTGGCCATGATCGTCGTCATGCTGATGCGTCCGCGCGGTCTCTGGCC
CTCGCCAGAGCACGCCAAGCCGCTGGTCAAACCTGTTAAAAGCTAA

Upstream 100 bases:

>100_bases
ATCGTGCTGATTTTTGTGCTCACGCTGCGCCCATCCGGCCTGCTGGGTGAACGTGTGGCTGACCGGGCCTGATCCCCGGT
TGCCCTAAGGAGAAATTCTG

Downstream 100 bases:

>100_bases
GGAGAATGGACATGAGCGATACCATTCTTAAAGTGGCCGGTGTTTCCAAGCGCTTTGGCGGCCTGCAGGCCTTGAGCGAC
GTCGGCATCCATATCAAGCG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKPNKLVTFFIVAAALLVLPLLLQQGGNAWVRIVDTALLYVLLALGLNIVVGYAGLLDLGYVAFFALGAYLFALLGSPHL
AEAFPVIAAAFPEGLHLPIWAAIPAAAALAGFFGVVLGAPTLKLRGDYLAIVTLGFGEIIRVFLNNLDRPINLTNGPKGI
NQIDSMKFFGFDLGKPHEFLGIEVNSVSMYYYLFLVLVIVSVIICHRLENSRIGRAWMAIREDEIAAKAMGINTRNMKLL
AFGMGATFGGVSGSMFAAFQGFVSPESFSLMESIMIVAMVVLGGIGHLPGVILGALLLAALPEVLRHVAGPLQAMTDGRL
DSAILRQLLIALAMIVVMLMRPRGLWPSPEHAKPLVKPVKS

Sequences:

>Translated_361_residues
MKPNKLVTFFIVAAALLVLPLLLQQGGNAWVRIVDTALLYVLLALGLNIVVGYAGLLDLGYVAFFALGAYLFALLGSPHL
AEAFPVIAAAFPEGLHLPIWAAIPAAAALAGFFGVVLGAPTLKLRGDYLAIVTLGFGEIIRVFLNNLDRPINLTNGPKGI
NQIDSMKFFGFDLGKPHEFLGIEVNSVSMYYYLFLVLVIVSVIICHRLENSRIGRAWMAIREDEIAAKAMGINTRNMKLL
AFGMGATFGGVSGSMFAAFQGFVSPESFSLMESIMIVAMVVLGGIGHLPGVILGALLLAALPEVLRHVAGPLQAMTDGRL
DSAILRQLLIALAMIVVMLMRPRGLWPSPEHAKPLVKPVKS
>Mature_361_residues
MKPNKLVTFFIVAAALLVLPLLLQQGGNAWVRIVDTALLYVLLALGLNIVVGYAGLLDLGYVAFFALGAYLFALLGSPHL
AEAFPVIAAAFPEGLHLPIWAAIPAAAALAGFFGVVLGAPTLKLRGDYLAIVTLGFGEIIRVFLNNLDRPINLTNGPKGI
NQIDSMKFFGFDLGKPHEFLGIEVNSVSMYYYLFLVLVIVSVIICHRLENSRIGRAWMAIREDEIAAKAMGINTRNMKLL
AFGMGATFGGVSGSMFAAFQGFVSPESFSLMESIMIVAMVVLGGIGHLPGVILGALLLAALPEVLRHVAGPLQAMTDGRL
DSAILRQLLIALAMIVVMLMRPRGLWPSPEHAKPLVKPVKS

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=341, Percent_Identity=39.8826979472141, Blast_Score=201, Evalue=6e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 38687; Mature: 38687

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPNKLVTFFIVAAALLVLPLLLQQGGNAWVRIVDTALLYVLLALGLNIVVGYAGLLDLG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVAFFALGAYLFALLGSPHLAEAFPVIAAAFPEGLHLPIWAAIPAAAALAGFFGVVLGAP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
TLKLRGDYLAIVTLGFGEIIRVFLNNLDRPINLTNGPKGINQIDSMKFFGFDLGKPHEFL
CEEECCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCHHCCCCCCCCCCEE
GIEVNSVSMYYYLFLVLVIVSVIICHRLENSRIGRAWMAIREDEIAAKAMGINTRNMKLL
EEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEE
AFGMGATFGGVSGSMFAAFQGFVSPESFSLMESIMIVAMVVLGGIGHLPGVILGALLLAA
EEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LPEVLRHVAGPLQAMTDGRLDSAILRQLLIALAMIVVMLMRPRGLWPSPEHAKPLVKPVK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCC
S
C
>Mature Secondary Structure
MKPNKLVTFFIVAAALLVLPLLLQQGGNAWVRIVDTALLYVLLALGLNIVVGYAGLLDLG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVAFFALGAYLFALLGSPHLAEAFPVIAAAFPEGLHLPIWAAIPAAAALAGFFGVVLGAP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
TLKLRGDYLAIVTLGFGEIIRVFLNNLDRPINLTNGPKGINQIDSMKFFGFDLGKPHEFL
CEEECCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCHHCCCCCCCCCCEE
GIEVNSVSMYYYLFLVLVIVSVIICHRLENSRIGRAWMAIREDEIAAKAMGINTRNMKLL
EEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEE
AFGMGATFGGVSGSMFAAFQGFVSPESFSLMESIMIVAMVVLGGIGHLPGVILGALLLAA
EEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LPEVLRHVAGPLQAMTDGRLDSAILRQLLIALAMIVVMLMRPRGLWPSPEHAKPLVKPVK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]