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The map label for this gene is paaF [C]

Identifier: 91789524

GI number: 91789524

Start: 3888879

End: 3889634

Strand: Direct

Name: paaF [C]

Synonym: Bpro_3677

Alternate gene names: 91789524

Gene position: 3888879-3889634 (Clockwise)

Preceding gene: 91789523

Following gene: 91789525

Centisome position: 74.78

GC content: 62.04

Gene sequence:

>756_bases
ATGTCACAAGTACTGTTTGAAAAACACGGGCCCGTCGCCATCGTCACGCTCAACCGCCCGGAGCGCCTGAATGCCATCAG
CGAAACGCTGCTGGACGACCTGCACGCCGCCCTGCTCAAGGCCCAGCTTGATGAGAGCATCAAGACCATTGTGCTTGCCG
GCGCAGGCCGGGCGTTTTGCGCGGGTGCCGACCTGAAGGAATTTTCCGGGCAAGCCGCCACCGCCCAGGACACATCGAGC
TATGCCGAGAAAATCCAGCAGGTCACGCGCGACATCATGTTCAGCGGCAAGCCCGTTGTCGGGGCCATCCAGGGGTTTGC
CGTGGGCGGGGGTTTCGAGTGGGTGCTCAACTGCGACATGGTGGTAGCGGCCGACGATGTGGTCTGTTTCTTCCCCGAAA
TGAGCTGGGGGCAGTTTGTGACCGGTGGCGTCACCCACCTGTTGCCCCAGGCTGTGGGCCATCAGCGTGCCATGGAACTC
TGGTTGCTGGGTGAAAAGCAAAGTGCCGACACGCTCTACCGGCTCGGCCTGGTCAACCGCGTGGTGCCCAAAGAGAAAGT
GCTGGAGACCGCGATCGCGCTGGCCGAAAAGATCAGCGAGCGCTCCACGTTCACCGTGTCGCGCCTGAAAAAGATGGTCA
ATACGCAACTGTCGGGTCAGCTGGCAACCGCCTTGCAGCTGGAAGCCCAGGCAGCGGCCAGTGCCATGGGGAACGAGGAC
ACCCAACAGCGCGTTGCGGGCTTTACCGCCCGATAA

Upstream 100 bases:

>100_bases
CCTGGGCTGAGGTGAGCCGAAAATAAGGGGGGCAGGTTGGGCCGCAAAAGGCTGCTGCCCACCGAACCCGACACACTGGA
AGAAACCATGGAGACAAGCC

Downstream 100 bases:

>100_bases
CCCGGGCAGCACCGCGCACCGCTGCAGCCTGTTTTTGAATCGCACCCCTGCAAAATCGCTCGTTTTCGGCTGGCGTAGCC
AGGCTTGGGCACGGGCGCTA

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MSQVLFEKHGPVAIVTLNRPERLNAISETLLDDLHAALLKAQLDESIKTIVLAGAGRAFCAGADLKEFSGQAATAQDTSS
YAEKIQQVTRDIMFSGKPVVGAIQGFAVGGGFEWVLNCDMVVAADDVVCFFPEMSWGQFVTGGVTHLLPQAVGHQRAMEL
WLLGEKQSADTLYRLGLVNRVVPKEKVLETAIALAEKISERSTFTVSRLKKMVNTQLSGQLATALQLEAQAAASAMGNED
TQQRVAGFTAR

Sequences:

>Translated_251_residues
MSQVLFEKHGPVAIVTLNRPERLNAISETLLDDLHAALLKAQLDESIKTIVLAGAGRAFCAGADLKEFSGQAATAQDTSS
YAEKIQQVTRDIMFSGKPVVGAIQGFAVGGGFEWVLNCDMVVAADDVVCFFPEMSWGQFVTGGVTHLLPQAVGHQRAMEL
WLLGEKQSADTLYRLGLVNRVVPKEKVLETAIALAEKISERSTFTVSRLKKMVNTQLSGQLATALQLEAQAAASAMGNED
TQQRVAGFTAR
>Mature_250_residues
SQVLFEKHGPVAIVTLNRPERLNAISETLLDDLHAALLKAQLDESIKTIVLAGAGRAFCAGADLKEFSGQAATAQDTSSY
AEKIQQVTRDIMFSGKPVVGAIQGFAVGGGFEWVLNCDMVVAADDVVCFFPEMSWGQFVTGGVTHLLPQAVGHQRAMELW
LLGEKQSADTLYRLGLVNRVVPKEKVLETAIALAEKISERSTFTVSRLKKMVNTQLSGQLATALQLEAQAAASAMGNEDT
QQRVAGFTAR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=244, Percent_Identity=34.4262295081967, Blast_Score=137, Evalue=7e-33,
Organism=Homo sapiens, GI68989263, Length=193, Percent_Identity=36.2694300518135, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI31542718, Length=238, Percent_Identity=29.8319327731092, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI70995211, Length=256, Percent_Identity=29.6875, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI37594469, Length=187, Percent_Identity=29.9465240641711, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI37594471, Length=187, Percent_Identity=29.9465240641711, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI20127408, Length=209, Percent_Identity=28.2296650717703, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI4502327, Length=252, Percent_Identity=26.5873015873016, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1787659, Length=250, Percent_Identity=32.4, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788597, Length=210, Percent_Identity=29.5238095238095, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787660, Length=242, Percent_Identity=33.0578512396694, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1788682, Length=218, Percent_Identity=32.1100917431193, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI221142681, Length=217, Percent_Identity=29.4930875576037, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI87082183, Length=202, Percent_Identity=27.2277227722772, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1790281, Length=217, Percent_Identity=27.1889400921659, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=249, Percent_Identity=33.3333333333333, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17554946, Length=240, Percent_Identity=31.6666666666667, Blast_Score=122, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17540714, Length=226, Percent_Identity=30.0884955752212, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17560910, Length=198, Percent_Identity=32.3232323232323, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17558304, Length=180, Percent_Identity=32.2222222222222, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25144276, Length=180, Percent_Identity=36.1111111111111, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17508951, Length=180, Percent_Identity=36.1111111111111, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17508953, Length=180, Percent_Identity=36.1111111111111, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=249, Percent_Identity=27.3092369477912, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=245, Percent_Identity=28.1632653061224, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=222, Percent_Identity=25.2252252252252, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=241, Percent_Identity=25.3112033195021, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=234, Percent_Identity=24.3589743589744, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25144157, Length=235, Percent_Identity=28.0851063829787, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI25144160, Length=235, Percent_Identity=28.0851063829787, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=197, Percent_Identity=26.9035532994924, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI20129971, Length=240, Percent_Identity=33.3333333333333, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24653477, Length=240, Percent_Identity=33.3333333333333, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24650670, Length=199, Percent_Identity=32.6633165829146, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21357171, Length=205, Percent_Identity=28.780487804878, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI19922422, Length=246, Percent_Identity=26.4227642276423, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19920382, Length=245, Percent_Identity=28.5714285714286, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24653139, Length=245, Percent_Identity=26.9387755102041, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24654903, Length=254, Percent_Identity=24.0157480314961, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571729, Length=195, Percent_Identity=27.6923076923077, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28571730, Length=195, Percent_Identity=27.6923076923077, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27009; Mature: 26878

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVLFEKHGPVAIVTLNRPERLNAISETLLDDLHAALLKAQLDESIKTIVLAGAGRAFC
CCCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEE
AGADLKEFSGQAATAQDTSSYAEKIQQVTRDIMFSGKPVVGAIQGFAVGGGFEWVLNCDM
CCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCEEECCCEEEEEECCE
VVAADDVVCFFPEMSWGQFVTGGVTHLLPQAVGHQRAMELWLLGEKQSADTLYRLGLVNR
EEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHH
VVPKEKVLETAIALAEKISERSTFTVSRLKKMVNTQLSGQLATALQLEAQAAASAMGNED
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCH
TQQRVAGFTAR
HHHHHHCCCCC
>Mature Secondary Structure 
SQVLFEKHGPVAIVTLNRPERLNAISETLLDDLHAALLKAQLDESIKTIVLAGAGRAFC
CCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEE
AGADLKEFSGQAATAQDTSSYAEKIQQVTRDIMFSGKPVVGAIQGFAVGGGFEWVLNCDM
CCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCEEECCCEEEEEECCE
VVAADDVVCFFPEMSWGQFVTGGVTHLLPQAVGHQRAMELWLLGEKQSADTLYRLGLVNR
EEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHH
VVPKEKVLETAIALAEKISERSTFTVSRLKKMVNTQLSGQLATALQLEAQAAASAMGNED
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCH
TQQRVAGFTAR
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA