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The map label for this gene is lysS [H]

Identifier: 91789471

GI number: 91789471

Start: 3823160

End: 3824710

Strand: Direct

Name: lysS [H]

Synonym: Bpro_3620

Alternate gene names: 91789471

Gene position: 3823160-3824710 (Clockwise)

Preceding gene: 91789470

Following gene: 91789473

Centisome position: 73.52

GC content: 61.32

Gene sequence:

>1551_bases
ATGTCCATCCACCCCGCTTTGCCCGCAGCCGACAATGCGATTCCCACCCCGCCAGACGACAACCAGCTGATGGTCGAGCG
GCGCGAAAAGCTCAAGGCCCTGCGCAGCGCCGGCGTCGCTTTTCCGAATGACGTCCGACCCGAGCACCGGGCCGAGGCAC
TGTTTGCGCAGTACGACAGCAAGACCAAGGAAGAGCTGGGACCTTTGGCCGTGAAGGTGAGCGTCGCCGGCCGCATGATG
CTCAAGCGGGTGATGGGAAAAGCCAGCTTTGCCACCCTGCAGGACGCTTCGTTTGGTCCGTCGGGTGGGCGCATCCAGGT
GTACATCAACAACGAGGGCGTGGGCGAAGAAGTTCACAACGCCTTCAAGCATTGGGACCTGGGCGACATCGTCGCGGCCA
CCGGTACGCTGTTCAAGACCAAGACCGGCGAGCTGTCGATTCATGCCACCAGTATCCGGCTGGTCACCAAAAGCCTGCGC
CCCCTGCCCGACAAATTCCACGGCATGGCCGACCAGGAAGTCAAATACCGCCAGCGCTATGTGGACCTGATCATGGACGA
GTCCGCCCGCAAGCGCTTCATGGCGCGCAGCAAGGCGGTGAGTGGCCTTCGCGAATTCATGGTGGCCCATGATTTCCTCG
AGGTGGAAACTCCCATGCTGCATCCGATTCCGGGTGGCGCCAATGCCAGGCCGTTCAAGACGCATCACAACGCGCTGGAC
CAGGAAATGTTCCTGCGTATCGCGCCCGAGCTTTACCTGAAACGCCTGATCGTTGGCGGCTTTGAACGTGTGTTCGAGAT
CAACCGCAGCTACCGCAACGAAGGCATCAGCGTGCGGCACAACCCCGAGTTCACCATGATGGAGTTCTACGCGGCCTGGT
GGAATTATTCGGACCTGATGACCTTCACCGAAGCCCTGATTCGGGACGCGGCCCAGAAGGTGACGGGCTCCCTGCAATTG
AGCTATGGCGGCAAGGCGGTTGACCTGAGCCAGCCCTTTGAGCGTCTCACCATTCGCGAAGCCATTCTGAAGCACACCGA
AGCCGGCGAGCACGTTGACGACATGGTGTGGCTGACCAACGCCCTGAAGAAACTCGGTGCAACCGAAGCCAAGGACCGCT
TGTCCACACGCTCGCTGGCCAGCCTGCAGGTCCTGTATTTTGAGGAAACGGTCGAGGAAAAACTCTGGCAGCCAACCTTC
ATCATGGAGCACCCGACCGAAATCTCGCCGCTGGCGCGCGCCAACGATGCGCGCCCCGAGGTCACGGAACGCTTTGAGCT
CTACATCACCGGCCGCGAATTCGGCAATGGTTTTTCCGAGCTGAACGATGCCGAGGAGCAGGCGGCCCGCTTCAACGCGC
AGGTGGCTGCCAAGGACGACGGCGACGACGAAGCCATGTTCTACGACCATGACTTCATCCGCGCCCTCGAATACGGCATG
CCGCCGACCGGCGGCTGCGGCGTGGGCATAGACCGGCTGATGATGCTACTGACCGACAGCCCCAGCATTCGCGATGTGAT
CTTGTTCCCCGCGCTACGCCGCGAAGCGTAA

Upstream 100 bases:

>100_bases
GCCGCTTAAAAACCTAAAATCATGGGTTCGGCGCCTTTCGGCCATTTTGAAATGGCGCAGCGCCCTGCACTTTCGACCTT
TTTCCGGAACACCCACCCCT

Downstream 100 bases:

>100_bases
GCGCGCCGCAGGGGCGCGCCTGCGCAGGCAACACGCCGGACAGGCCGGACCCGGTTCAGCCCTTGCGCTTTTTGAGCTTG
TGCAGGTAGTTGTCCAGAAC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MSIHPALPAADNAIPTPPDDNQLMVERREKLKALRSAGVAFPNDVRPEHRAEALFAQYDSKTKEELGPLAVKVSVAGRMM
LKRVMGKASFATLQDASFGPSGGRIQVYINNEGVGEEVHNAFKHWDLGDIVAATGTLFKTKTGELSIHATSIRLVTKSLR
PLPDKFHGMADQEVKYRQRYVDLIMDESARKRFMARSKAVSGLREFMVAHDFLEVETPMLHPIPGGANARPFKTHHNALD
QEMFLRIAPELYLKRLIVGGFERVFEINRSYRNEGISVRHNPEFTMMEFYAAWWNYSDLMTFTEALIRDAAQKVTGSLQL
SYGGKAVDLSQPFERLTIREAILKHTEAGEHVDDMVWLTNALKKLGATEAKDRLSTRSLASLQVLYFEETVEEKLWQPTF
IMEHPTEISPLARANDARPEVTERFELYITGREFGNGFSELNDAEEQAARFNAQVAAKDDGDDEAMFYDHDFIRALEYGM
PPTGGCGVGIDRLMMLLTDSPSIRDVILFPALRREA

Sequences:

>Translated_516_residues
MSIHPALPAADNAIPTPPDDNQLMVERREKLKALRSAGVAFPNDVRPEHRAEALFAQYDSKTKEELGPLAVKVSVAGRMM
LKRVMGKASFATLQDASFGPSGGRIQVYINNEGVGEEVHNAFKHWDLGDIVAATGTLFKTKTGELSIHATSIRLVTKSLR
PLPDKFHGMADQEVKYRQRYVDLIMDESARKRFMARSKAVSGLREFMVAHDFLEVETPMLHPIPGGANARPFKTHHNALD
QEMFLRIAPELYLKRLIVGGFERVFEINRSYRNEGISVRHNPEFTMMEFYAAWWNYSDLMTFTEALIRDAAQKVTGSLQL
SYGGKAVDLSQPFERLTIREAILKHTEAGEHVDDMVWLTNALKKLGATEAKDRLSTRSLASLQVLYFEETVEEKLWQPTF
IMEHPTEISPLARANDARPEVTERFELYITGREFGNGFSELNDAEEQAARFNAQVAAKDDGDDEAMFYDHDFIRALEYGM
PPTGGCGVGIDRLMMLLTDSPSIRDVILFPALRREA
>Mature_515_residues
SIHPALPAADNAIPTPPDDNQLMVERREKLKALRSAGVAFPNDVRPEHRAEALFAQYDSKTKEELGPLAVKVSVAGRMML
KRVMGKASFATLQDASFGPSGGRIQVYINNEGVGEEVHNAFKHWDLGDIVAATGTLFKTKTGELSIHATSIRLVTKSLRP
LPDKFHGMADQEVKYRQRYVDLIMDESARKRFMARSKAVSGLREFMVAHDFLEVETPMLHPIPGGANARPFKTHHNALDQ
EMFLRIAPELYLKRLIVGGFERVFEINRSYRNEGISVRHNPEFTMMEFYAAWWNYSDLMTFTEALIRDAAQKVTGSLQLS
YGGKAVDLSQPFERLTIREAILKHTEAGEHVDDMVWLTNALKKLGATEAKDRLSTRSLASLQVLYFEETVEEKLWQPTFI
MEHPTEISPLARANDARPEVTERFELYITGREFGNGFSELNDAEEQAARFNAQVAAKDDGDDEAMFYDHDFIRALEYGMP
PTGGCGVGIDRLMMLLTDSPSIRDVILFPALRREA

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=470, Percent_Identity=41.2765957446808, Blast_Score=347, Evalue=2e-95,
Organism=Homo sapiens, GI194272210, Length=470, Percent_Identity=41.2765957446808, Blast_Score=346, Evalue=4e-95,
Organism=Homo sapiens, GI40789249, Length=286, Percent_Identity=24.1258741258741, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1790571, Length=498, Percent_Identity=59.6385542168675, Blast_Score=606, Evalue=1e-174,
Organism=Escherichia coli, GI1789256, Length=498, Percent_Identity=59.0361445783133, Blast_Score=599, Evalue=1e-172,
Organism=Escherichia coli, GI87082379, Length=330, Percent_Identity=30.6060606060606, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1788173, Length=314, Percent_Identity=26.4331210191083, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=523, Percent_Identity=38.4321223709369, Blast_Score=337, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI17535927, Length=523, Percent_Identity=38.4321223709369, Blast_Score=336, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI71994340, Length=513, Percent_Identity=38.7914230019493, Blast_Score=334, Evalue=6e-92,
Organism=Caenorhabditis elegans, GI32566633, Length=251, Percent_Identity=27.8884462151394, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=525, Percent_Identity=39.2380952380952, Blast_Score=341, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6324256, Length=479, Percent_Identity=29.4363256784969, Blast_Score=180, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6323011, Length=404, Percent_Identity=23.7623762376238, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24640849, Length=505, Percent_Identity=39.6039603960396, Blast_Score=353, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24640851, Length=505, Percent_Identity=39.6039603960396, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24584738, Length=292, Percent_Identity=26.7123287671233, Blast_Score=79, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 58128; Mature: 57997

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIHPALPAADNAIPTPPDDNQLMVERREKLKALRSAGVAFPNDVRPEHRAEALFAQYDS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCC
KTKEELGPLAVKVSVAGRMMLKRVMGKASFATLQDASFGPSGGRIQVYINNEGVGEEVHN
HHHHHHCCEEEEEHHHHHHHHHHHHCCHHHHECCCCCCCCCCCEEEEEECCCCCCHHHHH
AFKHWDLGDIVAATGTLFKTKTGELSIHATSIRLVTKSLRPLPDKFHGMADQEVKYRQRY
HHHCCCCHHHHHHCCCEEEECCCCEEEEEHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHH
VDLIMDESARKRFMARSKAVSGLREFMVAHDFLEVETPMLHPIPGGANARPFKTHHNALD
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
QEMFLRIAPELYLKRLIVGGFERVFEINRSYRNEGISVRHNPEFTMMEFYAAWWNYSDLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCHHHHH
TFTEALIRDAAQKVTGSLQLSYGGKAVDLSQPFERLTIREAILKHTEAGEHVDDMVWLTN
HHHHHHHHHHHHHHHCCEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ALKKLGATEAKDRLSTRSLASLQVLYFEETVEEKLWQPTFIMEHPTEISPLARANDARPE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHCCCCCCC
VTERFELYITGREFGNGFSELNDAEEQAARFNAQVAAKDDGDDEAMFYDHDFIRALEYGM
HHCEEEEEEECHHHCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCEEECHHHHHHHHHCC
PPTGGCGVGIDRLMMLLTDSPSIRDVILFPALRREA
CCCCCCCCCHHHHHHHHCCCCCCCCEEECCHHHCCC
>Mature Secondary Structure 
SIHPALPAADNAIPTPPDDNQLMVERREKLKALRSAGVAFPNDVRPEHRAEALFAQYDS
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCC
KTKEELGPLAVKVSVAGRMMLKRVMGKASFATLQDASFGPSGGRIQVYINNEGVGEEVHN
HHHHHHCCEEEEEHHHHHHHHHHHHCCHHHHECCCCCCCCCCCEEEEEECCCCCCHHHHH
AFKHWDLGDIVAATGTLFKTKTGELSIHATSIRLVTKSLRPLPDKFHGMADQEVKYRQRY
HHHCCCCHHHHHHCCCEEEECCCCEEEEEHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHH
VDLIMDESARKRFMARSKAVSGLREFMVAHDFLEVETPMLHPIPGGANARPFKTHHNALD
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
QEMFLRIAPELYLKRLIVGGFERVFEINRSYRNEGISVRHNPEFTMMEFYAAWWNYSDLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCHHHHH
TFTEALIRDAAQKVTGSLQLSYGGKAVDLSQPFERLTIREAILKHTEAGEHVDDMVWLTN
HHHHHHHHHHHHHHHCCEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ALKKLGATEAKDRLSTRSLASLQVLYFEETVEEKLWQPTFIMEHPTEISPLARANDARPE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHCCCCCCC
VTERFELYITGREFGNGFSELNDAEEQAARFNAQVAAKDDGDDEAMFYDHDFIRALEYGM
HHCEEEEEEECHHHCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCEEECHHHHHHHHHCC
PPTGGCGVGIDRLMMLLTDSPSIRDVILFPALRREA
CCCCCCCCCHHHHHHHHCCCCCCCCEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA