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The map label for this gene is fixL [H]

Identifier: 91789362

GI number: 91789362

Start: 3708586

End: 3710250

Strand: Direct

Name: fixL [H]

Synonym: Bpro_3508

Alternate gene names: 91789362

Gene position: 3708586-3710250 (Clockwise)

Preceding gene: 91789361

Following gene: 91789363

Centisome position: 71.32

GC content: 61.08

Gene sequence:

>1665_bases
ATGGATGCTCGTGCCAAGACAGTAAAGGAGCGCAATGGCGCGCCAATAACGCCACCAGGAGAGCAATCGGTTTTCCTGCA
AAACATCCTCGAATCGTCGACCGAGTATTCGATCATCGGCAAGGATCTCAGCGGCAAGATCCAGCTCTGGAACGCCGGTG
CACGGCGCCTCTATGGCTACGAGCCGGAAGAAGTCATTGGCAAGGCCAACTCGTCCATGCTCCATACGCCGGAGGACCTT
GCCGCCGGCAAGCCGGCCCAGATCATGGACGCCGCCTTGCAAGCCGGCAAATGGGAAGGTACCCTCACGCGATGCCGCAA
TAACGGCGAGCGATTCACCGCGCGCGTCGTCATCACGCCACGCCGCGATGCCACCGGCAAGCCCATCGGCTTCCTGCTGA
TCTCGAAAGACATCTCGGATGAAATCCGCCTGACGGAAGAACTCAAGGCCACGCAGTTCTACGCGCGCAGCCTGATCGAG
GCCTCGCTCGACCCGCTGGTCACCATCAGCCCTGAAGGCAAGATCACCGATGTCAATGAAGGCTCGATCAAGGTCACCGG
CGTGCCGCGCGAGAAGTTGATCGGAGCCGACTTCTCCGACTACTTCACCGAGCCGGAAAAGGCCCGCGAAGGCTACCAGC
AGGTTTTTTCCAAGGGTGCCGTCACCGACTACCCCCTGACGATCCGGCACAAGGACGGGCGCCTCACCGACGTGCTCTAC
AACGCCTCGGTGTACAAGGACGCGCGCGGAAATGTGCTGGGCGTGTTTGCGGCAGCGCGCGATGTGACGGAAAGCCGCAG
GGCGGAACAGAGATTCCGGACCCTCATCGAGTCGGCGCCGGATGCGATGGTGCTTGCCAACCGGGAAGGAATCATCCTGC
TGGTGAACAACCAGACCGAAAGCCTGTTCGGCTATGCGCGCGAAGAGATGCTTGGGAAGTCGGTTGATCTCTTCGTTCCC
AGCCGCTACCGCAGCGCCCATCCTGGAAATCGGGCGAAGTTCTTCGGCGAACCGCGCCTGCGTCCCATGGGCGCGGGCCT
GGAACTCTGGGGTGCTCGCAAGGACGGAAGCGAGTTCCCGATCGAGATCAGTCTCAGCCCGATCGAAAGCCCCCAGGGTT
TGTCTGTCGCGGCGACGATTCGGGATGTCACGGTGCAAAAGACATCCTCCCAATATGCCCGCAGCCTGATCGAGGCTTCG
CTCGATCCACTGGTCACCATCAGCACGGAAGGCAAGATCACCGACGTCAATGAGGGATCGATCAAGGTCACCGGCATCTC
GCGCGACAAGCTGATCGGGACCGACTTCTCCGACTACTTTACCGAACCGAAAAAAGCGCGCGAAGGCTACCAGCAGGTGT
TCGCCGAGGGCTTCGTGACCGACTACCCGCTGACAATCCGACACAAGGACGGGCGCCTCACCGACGTGCTCTACAACGCC
TCAGTCTACAAGGACACACTCGGCAACGTGTTGGGCGTGTTCGCCGCCGCGCGTGACGTGACGGCGCAGAAGAGGGCCGA
GGCCGAGGTCGCCGAACAGCGCAACAAGGAGTTGGAGCGGCTGGCGGAACTGGAGCGTTTCCAGCGGCTTACCGTCGGAC
GGGAGCTCAAGATGATCGAGCTCAAGAAGGAGATTGAAGAACTCAAGAAAACCGGGGCCAGGTGA

Upstream 100 bases:

>100_bases
CGCGTAGCCGCACGAGCCGATGGTGTGGCACCCAGCATTTTCATCGAGGCGCGATAGCGACCGCGGCTCATTCGCCAACC
CCTCTCGGGAGGAGCCTCAA

Downstream 100 bases:

>100_bases
CAGACTATGCCGGCGAACATGGCGCACAGCGTGGACACCGAGCAGCCGTTCAGTACCGCCGACTATGCGAAGGCGGTTCT
CAATATTCTTGAAGACGCCG

Product: putative PAS/PAC sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MDARAKTVKERNGAPITPPGEQSVFLQNILESSTEYSIIGKDLSGKIQLWNAGARRLYGYEPEEVIGKANSSMLHTPEDL
AAGKPAQIMDAALQAGKWEGTLTRCRNNGERFTARVVITPRRDATGKPIGFLLISKDISDEIRLTEELKATQFYARSLIE
ASLDPLVTISPEGKITDVNEGSIKVTGVPREKLIGADFSDYFTEPEKAREGYQQVFSKGAVTDYPLTIRHKDGRLTDVLY
NASVYKDARGNVLGVFAAARDVTESRRAEQRFRTLIESAPDAMVLANREGIILLVNNQTESLFGYAREEMLGKSVDLFVP
SRYRSAHPGNRAKFFGEPRLRPMGAGLELWGARKDGSEFPIEISLSPIESPQGLSVAATIRDVTVQKTSSQYARSLIEAS
LDPLVTISTEGKITDVNEGSIKVTGISRDKLIGTDFSDYFTEPKKAREGYQQVFAEGFVTDYPLTIRHKDGRLTDVLYNA
SVYKDTLGNVLGVFAAARDVTAQKRAEAEVAEQRNKELERLAELERFQRLTVGRELKMIELKKEIEELKKTGAR

Sequences:

>Translated_554_residues
MDARAKTVKERNGAPITPPGEQSVFLQNILESSTEYSIIGKDLSGKIQLWNAGARRLYGYEPEEVIGKANSSMLHTPEDL
AAGKPAQIMDAALQAGKWEGTLTRCRNNGERFTARVVITPRRDATGKPIGFLLISKDISDEIRLTEELKATQFYARSLIE
ASLDPLVTISPEGKITDVNEGSIKVTGVPREKLIGADFSDYFTEPEKAREGYQQVFSKGAVTDYPLTIRHKDGRLTDVLY
NASVYKDARGNVLGVFAAARDVTESRRAEQRFRTLIESAPDAMVLANREGIILLVNNQTESLFGYAREEMLGKSVDLFVP
SRYRSAHPGNRAKFFGEPRLRPMGAGLELWGARKDGSEFPIEISLSPIESPQGLSVAATIRDVTVQKTSSQYARSLIEAS
LDPLVTISTEGKITDVNEGSIKVTGISRDKLIGTDFSDYFTEPKKAREGYQQVFAEGFVTDYPLTIRHKDGRLTDVLYNA
SVYKDTLGNVLGVFAAARDVTAQKRAEAEVAEQRNKELERLAELERFQRLTVGRELKMIELKKEIEELKKTGAR
>Mature_554_residues
MDARAKTVKERNGAPITPPGEQSVFLQNILESSTEYSIIGKDLSGKIQLWNAGARRLYGYEPEEVIGKANSSMLHTPEDL
AAGKPAQIMDAALQAGKWEGTLTRCRNNGERFTARVVITPRRDATGKPIGFLLISKDISDEIRLTEELKATQFYARSLIE
ASLDPLVTISPEGKITDVNEGSIKVTGVPREKLIGADFSDYFTEPEKAREGYQQVFSKGAVTDYPLTIRHKDGRLTDVLY
NASVYKDARGNVLGVFAAARDVTESRRAEQRFRTLIESAPDAMVLANREGIILLVNNQTESLFGYAREEMLGKSVDLFVP
SRYRSAHPGNRAKFFGEPRLRPMGAGLELWGARKDGSEFPIEISLSPIESPQGLSVAATIRDVTVQKTSSQYARSLIEAS
LDPLVTISTEGKITDVNEGSIKVTGISRDKLIGTDFSDYFTEPKKAREGYQQVFAEGFVTDYPLTIRHKDGRLTDVLYNA
SVYKDTLGNVLGVFAAARDVTAQKRAEAEVAEQRNKELERLAELERFQRLTVGRELKMIELKKEIEELKKTGAR

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI87081921, Length=149, Percent_Identity=30.8724832214765, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 61453; Mature: 61453

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS50112 PAS ; PS50113 PAC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDARAKTVKERNGAPITPPGEQSVFLQNILESSTEYSIIGKDLSGKIQLWNAGARRLYGY
CCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCHHCCCC
EPEEVIGKANSSMLHTPEDLAAGKPAQIMDAALQAGKWEGTLTRCRNNGERFTARVVITP
CHHHHHCCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCHHHHHCCCCCEEEEEEEEEC
RRDATGKPIGFLLISKDISDEIRLTEELKATQFYARSLIEASLDPLVTISPEGKITDVNE
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCC
GSIKVTGVPREKLIGADFSDYFTEPEKAREGYQQVFSKGAVTDYPLTIRHKDGRLTDVLY
CEEEEECCCHHHHCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEE
NASVYKDARGNVLGVFAAARDVTESRRAEQRFRTLIESAPDAMVLANREGIILLVNNQTE
CCHHEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCH
SLFGYAREEMLGKSVDLFVPSRYRSAHPGNRAKFFGEPRLRPMGAGLELWGARKDGSEFP
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEECCCCCCCCCCCCEEEEECCCCCCCCC
IEISLSPIESPQGLSVAATIRDVTVQKTSSQYARSLIEASLDPLVTISTEGKITDVNEGS
EEEEECCCCCCCCCEEEEEEEHHEEEHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCE
IKVTGISRDKLIGTDFSDYFTEPKKAREGYQQVFAEGFVTDYPLTIRHKDGRLTDVLYNA
EEEEECCCCCEECCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEECC
SVYKDTLGNVLGVFAAARDVTAQKRAEAEVAEQRNKELERLAELERFQRLTVGRELKMIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LKKEIEELKKTGAR
HHHHHHHHHHCCCC
>Mature Secondary Structure
MDARAKTVKERNGAPITPPGEQSVFLQNILESSTEYSIIGKDLSGKIQLWNAGARRLYGY
CCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCHHCCCC
EPEEVIGKANSSMLHTPEDLAAGKPAQIMDAALQAGKWEGTLTRCRNNGERFTARVVITP
CHHHHHCCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCHHHHHCCCCCEEEEEEEEEC
RRDATGKPIGFLLISKDISDEIRLTEELKATQFYARSLIEASLDPLVTISPEGKITDVNE
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCC
GSIKVTGVPREKLIGADFSDYFTEPEKAREGYQQVFSKGAVTDYPLTIRHKDGRLTDVLY
CEEEEECCCHHHHCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEE
NASVYKDARGNVLGVFAAARDVTESRRAEQRFRTLIESAPDAMVLANREGIILLVNNQTE
CCHHEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCH
SLFGYAREEMLGKSVDLFVPSRYRSAHPGNRAKFFGEPRLRPMGAGLELWGARKDGSEFP
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEECCCCCCCCCCCCEEEEECCCCCCCCC
IEISLSPIESPQGLSVAATIRDVTVQKTSSQYARSLIEASLDPLVTISTEGKITDVNEGS
EEEEECCCCCCCCCEEEEEEEHHEEEHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCE
IKVTGISRDKLIGTDFSDYFTEPKKAREGYQQVFAEGFVTDYPLTIRHKDGRLTDVLYNA
EEEEECCCCCEECCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEECC
SVYKDTLGNVLGVFAAARDVTAQKRAEAEVAEQRNKELERLAELERFQRLTVGRELKMIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LKKEIEELKKTGAR
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2046550; 1766374 [H]