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The map label for this gene is cueO [H]

Identifier: 91789358

GI number: 91789358

Start: 3701717

End: 3703372

Strand: Direct

Name: cueO [H]

Synonym: Bpro_3504

Alternate gene names: 91789358

Gene position: 3701717-3703372 (Clockwise)

Preceding gene: 91789357

Following gene: 91789359

Centisome position: 71.18

GC content: 66.06

Gene sequence:

>1656_bases
ATGAACAATCAAAGGCGCCGCATCCTGATCCGTTCAGCCGCCACTGCGGCGGTCGCTTCGCTCGGCCTGCGGGCGTTAAG
CACAAGGGCCGGCAACGAAGAGCACACCGCCGCCGGCATCGATGTGCACATCGAACTGCATGCGGTTCAAGATAAGGTTG
CCATCCGCCCGGGTGAGCAGACCCAGGTGTGGCGCTACCGGGGCAGGCTGCTGCACGGCGACGCGAGCGCGCTCAATACG
TGGGCCAGCAACTACCTGGGGCCCATCATCCGAGTGCGCCGGGGTCAGCGGGTGCGCATCGACCTGATCAACGCGCTGCC
TGAGTCGACCATCACTCATTGGCACGGACTGCACGTGCCGGAGGCGATGGATGGCCATCCGCGCTACGCGATTGCGCCGG
GCGAGCGCTACGTCTACGAGTTCACGGTCGTGGATCGGGCCGGCAGCTACTGGTTCCACCCGCACCCCCATGGCCGGACG
GGCAAGCAGATCTACTTCGGCCTGGCGGGCCTGTTCCTGGTAAGCGACGACGAGGAGGCTGCCCTTGATCTGCCCACCGG
CCCGCACGACCTTCCGCTGGTGATCCAGGACCGCACGTTCGACGACGACAACCAGCTCGTCTACCTGTCGGAGAGCGCGG
CCGGTGCTGTATCGCAGGGCACTGGCCAACACGGGATGATGGGCGGCGGCATGATGGGTGGAGACGGCCGCAGCGGTGCC
ATGGGTGCCATGGATGCCATGGGCGGCATGGGCCAGATGATGGCCCGCATGATGGGCGTGCTCGGGGACAATATCCTCGT
TAACGGCAGACCCGGCGCGAACCTGGAGGTCGAGCGCCGCCCCCATCGCCTGCGCTTGCTCAATGCCTCGAACACTCGCA
TGTACAAGCTGGCGTGGCATGACCAGTCGCCGCTGACGGTGATCGCGACCGACGGCGGCTTGCTGGCGGCGCCGCTGCAA
CGCGACTACGTCATGCTCTCGCCGGCCGAGCGCGTCGACTTGTGGGTGGACTTCGGCCGCTGGACCGCGGGCACAGAACT
GACGCTGCAAAGCCTCGCCTTCGCGGGCGGCATGGCCATGGGCGGAATGATGGATGGAACGGCGCTGCCCGATGGCGCGC
CGTTCCCAGTGCTCAAGCTGCGGGTCGAGGGTGGCACGAGAGGCCGCGCCGCGCTGCCACAACGCCTGTCCCAGTTGCGG
CGCCCGCAACCGCGCGCTGCCGTCAACTTCAACCACCCCAAGGTGTTCAATCTCACCATGGGCATGATGGTCTGGGGCAT
CAACGGCCAAAGCTTCGACATGCTCGGTGCGAGCCCGCTTGAAACCGTCAAGCTGGGCACCCACGAGGTCTGGGAGTTTC
GCAACGATGCCGCGAGCGGGATGATGGATATGACGATGCCCCACTCGATGCACGTGCACGGGTTGCAGTTCCGGGTCATC
GGGCGCTCCGTTGCCGGCAAGTTCTCGCGGCAACACGGCACGGTGAAGGCCGGCTTCGTCGACGAAGGGTGGAAGGATAC
GGTGCTGGTGATGCCGGGCGAGCGCGTGCGCCTCCTGCTGGGCTTCACCGACTACCCGGGGCTGTTTCCCTACCACTGCC
ACATGCTGGAGCACGAGGACTCCGGCTTGATGCGCAACTACCTCGTCAAGGAGTAG

Upstream 100 bases:

>100_bases
GCGCCATCGGCATGGCGGCGATGCATTTCGGAATGATGGGTCGCTGGAGCTGCTGACAGGTCCCGTTCCCCGCACCAAAA
CCAGATCGGAGGCGTCCCAC

Downstream 100 bases:

>100_bases
ACGATGATGGAGCGTCTGCTGATGGTCATCGCGGCGGCGACCGTGGCAACGGTTGCCCGCAGCGACGACGCCGCGGTGCG
CGCCACGTTGCAGCGCACCT

Product: multicopper oxidase, type 3

Products: NA

Alternate protein names: Copper efflux oxidase [H]

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MNNQRRRILIRSAATAAVASLGLRALSTRAGNEEHTAAGIDVHIELHAVQDKVAIRPGEQTQVWRYRGRLLHGDASALNT
WASNYLGPIIRVRRGQRVRIDLINALPESTITHWHGLHVPEAMDGHPRYAIAPGERYVYEFTVVDRAGSYWFHPHPHGRT
GKQIYFGLAGLFLVSDDEEAALDLPTGPHDLPLVIQDRTFDDDNQLVYLSESAAGAVSQGTGQHGMMGGGMMGGDGRSGA
MGAMDAMGGMGQMMARMMGVLGDNILVNGRPGANLEVERRPHRLRLLNASNTRMYKLAWHDQSPLTVIATDGGLLAAPLQ
RDYVMLSPAERVDLWVDFGRWTAGTELTLQSLAFAGGMAMGGMMDGTALPDGAPFPVLKLRVEGGTRGRAALPQRLSQLR
RPQPRAAVNFNHPKVFNLTMGMMVWGINGQSFDMLGASPLETVKLGTHEVWEFRNDAASGMMDMTMPHSMHVHGLQFRVI
GRSVAGKFSRQHGTVKAGFVDEGWKDTVLVMPGERVRLLLGFTDYPGLFPYHCHMLEHEDSGLMRNYLVKE

Sequences:

>Translated_551_residues
MNNQRRRILIRSAATAAVASLGLRALSTRAGNEEHTAAGIDVHIELHAVQDKVAIRPGEQTQVWRYRGRLLHGDASALNT
WASNYLGPIIRVRRGQRVRIDLINALPESTITHWHGLHVPEAMDGHPRYAIAPGERYVYEFTVVDRAGSYWFHPHPHGRT
GKQIYFGLAGLFLVSDDEEAALDLPTGPHDLPLVIQDRTFDDDNQLVYLSESAAGAVSQGTGQHGMMGGGMMGGDGRSGA
MGAMDAMGGMGQMMARMMGVLGDNILVNGRPGANLEVERRPHRLRLLNASNTRMYKLAWHDQSPLTVIATDGGLLAAPLQ
RDYVMLSPAERVDLWVDFGRWTAGTELTLQSLAFAGGMAMGGMMDGTALPDGAPFPVLKLRVEGGTRGRAALPQRLSQLR
RPQPRAAVNFNHPKVFNLTMGMMVWGINGQSFDMLGASPLETVKLGTHEVWEFRNDAASGMMDMTMPHSMHVHGLQFRVI
GRSVAGKFSRQHGTVKAGFVDEGWKDTVLVMPGERVRLLLGFTDYPGLFPYHCHMLEHEDSGLMRNYLVKE
>Mature_551_residues
MNNQRRRILIRSAATAAVASLGLRALSTRAGNEEHTAAGIDVHIELHAVQDKVAIRPGEQTQVWRYRGRLLHGDASALNT
WASNYLGPIIRVRRGQRVRIDLINALPESTITHWHGLHVPEAMDGHPRYAIAPGERYVYEFTVVDRAGSYWFHPHPHGRT
GKQIYFGLAGLFLVSDDEEAALDLPTGPHDLPLVIQDRTFDDDNQLVYLSESAAGAVSQGTGQHGMMGGGMMGGDGRSGA
MGAMDAMGGMGQMMARMMGVLGDNILVNGRPGANLEVERRPHRLRLLNASNTRMYKLAWHDQSPLTVIATDGGLLAAPLQ
RDYVMLSPAERVDLWVDFGRWTAGTELTLQSLAFAGGMAMGGMMDGTALPDGAPFPVLKLRVEGGTRGRAALPQRLSQLR
RPQPRAAVNFNHPKVFNLTMGMMVWGINGQSFDMLGASPLETVKLGTHEVWEFRNDAASGMMDMTMPHSMHVHGLQFRVI
GRSVAGKFSRQHGTVKAGFVDEGWKDTVLVMPGERVRLLLGFTDYPGLFPYHCHMLEHEDSGLMRNYLVKE

Specific function: Probably involved in periplasmic detoxification of copper by oxidizing Cu(+) to Cu(2+) and thus preventing its uptake into the cytoplasm. Possesses phenoloxidase and ferroxidase activities and might be involved in the production of polyphenolic compounds

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 plastocyanin-like domains [H]

Homologues:

Organism=Escherichia coli, GI1786314, Length=521, Percent_Identity=30.7101727447217, Blast_Score=186, Evalue=5e-48,
Organism=Escherichia coli, GI1789394, Length=511, Percent_Identity=28.1800391389432, Blast_Score=145, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6323703, Length=117, Percent_Identity=38.4615384615385, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24650186, Length=278, Percent_Identity=26.2589928057554, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011706
- InterPro:   IPR011707
- InterPro:   IPR002355
- InterPro:   IPR008972
- InterPro:   IPR006311 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07731 Cu-oxidase_2; PF07732 Cu-oxidase_3 [H]

EC number: NA

Molecular weight: Translated: 60590; Mature: 60590

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS00079 MULTICOPPER_OXIDASE1 ; PS00080 MULTICOPPER_OXIDASE2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNQRRRILIRSAATAAVASLGLRALSTRAGNEEHTAAGIDVHIELHAVQDKVAIRPGEQ
CCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEECCEEEECCCCC
TQVWRYRGRLLHGDASALNTWASNYLGPIIRVRRGQRVRIDLINALPESTITHWHGLHVP
CHHEEECCEEEECCHHHHHHHHHHHHCHHHEECCCCEEEEEEECCCCCHHHHHCCCCCCC
EAMDGHPRYAIAPGERYVYEFTVVDRAGSYWFHPHPHGRTGKQIYFGLAGLFLVSDDEEA
CCCCCCCCEEECCCCEEEEEEEEEECCCCEEECCCCCCCCCCEEEEEEEEEEEEECCCCC
ALDLPTGPHDLPLVIQDRTFDDDNQLVYLSESAAGAVSQGTGQHGMMGGGMMGGDGRSGA
EEECCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEECCCCCCCC
MGAMDAMGGMGQMMARMMGVLGDNILVNGRPGANLEVERRPHRLRLLNASNTRMYKLAWH
CHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCEEEEEECCCCEEEEEEEC
DQSPLTVIATDGGLLAAPLQRDYVMLSPAERVDLWVDFGRWTAGTELTLQSLAFAGGMAM
CCCCEEEEEECCCEEECCCCCCEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHCCHHC
GGMMDGTALPDGAPFPVLKLRVEGGTRGRAALPQRLSQLRRPQPRAAVNFNHPKVFNLTM
CCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCEEEEEEE
GMMVWGINGQSFDMLGASPLETVKLGTHEVWEFRNDAASGMMDMTMPHSMHVHGLQFRVI
EEEEECCCCCCEECCCCCCCCEEECCCHHHHHHHCHHHCCEEEECCCCCEEEEEEEEEEE
GRSVAGKFSRQHGTVKAGFVDEGWKDTVLVMPGERVRLLLGFTDYPGLFPYHCHMLEHED
CHHHHHHHHHCCCCEEECCCCCCCCCEEEEECCCEEEEEEEECCCCCCCCEEEEEEEECC
SGLMRNYLVKE
CCHHHHHHCCC
>Mature Secondary Structure
MNNQRRRILIRSAATAAVASLGLRALSTRAGNEEHTAAGIDVHIELHAVQDKVAIRPGEQ
CCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEECCEEEECCCCC
TQVWRYRGRLLHGDASALNTWASNYLGPIIRVRRGQRVRIDLINALPESTITHWHGLHVP
CHHEEECCEEEECCHHHHHHHHHHHHCHHHEECCCCEEEEEEECCCCCHHHHHCCCCCCC
EAMDGHPRYAIAPGERYVYEFTVVDRAGSYWFHPHPHGRTGKQIYFGLAGLFLVSDDEEA
CCCCCCCCEEECCCCEEEEEEEEEECCCCEEECCCCCCCCCCEEEEEEEEEEEEECCCCC
ALDLPTGPHDLPLVIQDRTFDDDNQLVYLSESAAGAVSQGTGQHGMMGGGMMGGDGRSGA
EEECCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEECCCCCCCC
MGAMDAMGGMGQMMARMMGVLGDNILVNGRPGANLEVERRPHRLRLLNASNTRMYKLAWH
CHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCEEEEEECCCCEEEEEEEC
DQSPLTVIATDGGLLAAPLQRDYVMLSPAERVDLWVDFGRWTAGTELTLQSLAFAGGMAM
CCCCEEEEEECCCEEECCCCCCEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHCCHHC
GGMMDGTALPDGAPFPVLKLRVEGGTRGRAALPQRLSQLRRPQPRAAVNFNHPKVFNLTM
CCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCEEEEEEE
GMMVWGINGQSFDMLGASPLETVKLGTHEVWEFRNDAASGMMDMTMPHSMHVHGLQFRVI
EEEEECCCCCCEECCCCCCCCEEECCCHHHHHHHCHHHCCEEEECCCCCEEEEEEEEEEE
GRSVAGKFSRQHGTVKAGFVDEGWKDTVLVMPGERVRLLLGFTDYPGLFPYHCHMLEHED
CHHHHHHHHHCCCCEEECCCCCCCCCEEEEECCCEEEEEEEECCCCCCCCEEEEEEEECC
SGLMRNYLVKE
CCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]