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The map label for this gene is pbuE [H]

Identifier: 91789331

GI number: 91789331

Start: 3679410

End: 3680018

Strand: Direct

Name: pbuE [H]

Synonym: Bpro_3476

Alternate gene names: 91789331

Gene position: 3679410-3680018 (Clockwise)

Preceding gene: 91789330

Following gene: 91789332

Centisome position: 70.75

GC content: 67.49

Gene sequence:

>609_bases
ATGCCCCCCGTTCTCTATTTTTCCGCGTTGTGCAACCTCGTCATTGGCAGCGGCGCTTTTGTGCTGGGCGGCATTCTGGA
GCCGATGAGCGAATCGCTGAACATCAGCGTGGCCGCAGCGGGCCAGGCCATGACCGCCTACGCCACCGCCACGGCCGTGC
TGGCACCGCTGCTGATCATCCTGACCGCCAGATGGACCCGCAAGCGCGCCGTCCAGCTGGCGCTGTTGCTCTTTGCAACG
GACTGCCTGCTGTGTGCACTGGCGCCCAGCCTGGCTGTCCTTCTGCTGGGGCGGGTGCTGATGGGTGCGGGCGCCATGTT
CACGGCAGCCGCCTCCGCGCTGGCCGTCAGCATGGTGGCGCCCTCGCAGCGCGGCCGTGCGCTGTCCATCACCTTCCTCG
GCATGAGCATCAGTTACGCCGTGGGGCTGCCAGTGGATGCCTGGCTGGGTTTTGAATACGGCTGGCGCGTGCCGGTGTGG
CTGTCGGCCGCGGCCAGCTGGCTGATCCCGGCCACGATGGCCAGTGCTTTGGCCGCATCGATGGCCCGTGGCGAGCAGGC
CGGTCGTGACGAAGCATCGGCCAAACCCGCCTCACCCAGCGCCAGGTAA

Upstream 100 bases:

>100_bases
GTTTCGATGCCGTCCGCGCAAGCCATCGGGCACTGATCTTCCTCGTCCCCTCTTCCCTCTTCCCTCTTCCCTCTTCCCTC
TTCCCTCTTCCCTAATATCC

Downstream 100 bases:

>100_bases
CGTCCCAGCCACTTGCGTGCCGTTGGGGCTGTGACCCCGTGCATCCGGGCGGCATTGCAGGCGCTCAGCCCACGCAAAGT
AATGTCTTTGAGCATCTCGA

Product: MFS transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MPPVLYFSALCNLVIGSGAFVLGGILEPMSESLNISVAAAGQAMTAYATATAVLAPLLIILTARWTRKRAVQLALLLFAT
DCLLCALAPSLAVLLLGRVLMGAGAMFTAAASALAVSMVAPSQRGRALSITFLGMSISYAVGLPVDAWLGFEYGWRVPVW
LSAAASWLIPATMASALAASMARGEQAGRDEASAKPASPSAR

Sequences:

>Translated_202_residues
MPPVLYFSALCNLVIGSGAFVLGGILEPMSESLNISVAAAGQAMTAYATATAVLAPLLIILTARWTRKRAVQLALLLFAT
DCLLCALAPSLAVLLLGRVLMGAGAMFTAAASALAVSMVAPSQRGRALSITFLGMSISYAVGLPVDAWLGFEYGWRVPVW
LSAAASWLIPATMASALAASMARGEQAGRDEASAKPASPSAR
>Mature_201_residues
PPVLYFSALCNLVIGSGAFVLGGILEPMSESLNISVAAAGQAMTAYATATAVLAPLLIILTARWTRKRAVQLALLLFATD
CLLCALAPSLAVLLLGRVLMGAGAMFTAAASALAVSMVAPSQRGRALSITFLGMSISYAVGLPVDAWLGFEYGWRVPVWL
SAAASWLIPATMASALAASMARGEQAGRDEASAKPASPSAR

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine and 6-mercaptopurine. Therefore plays a role in maintaining t

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787947, Length=183, Percent_Identity=30.6010928961749, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI145693198, Length=134, Percent_Identity=35.8208955223881, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786595, Length=197, Percent_Identity=25.8883248730964, Blast_Score=61, Evalue=5e-11,
Organism=Escherichia coli, GI1787808, Length=149, Percent_Identity=26.1744966442953, Blast_Score=60, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 20758; Mature: 20627

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPVLYFSALCNLVIGSGAFVLGGILEPMSESLNISVAAAGQAMTAYATATAVLAPLLII
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHH
LTARWTRKRAVQLALLLFATDCLLCALAPSLAVLLLGRVLMGAGAMFTAAASALAVSMVA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
PSQRGRALSITFLGMSISYAVGLPVDAWLGFEYGWRVPVWLSAAASWLIPATMASALAAS
CCCCCCEEEEEEEEEEEEHEECCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MARGEQAGRDEASAKPASPSAR
HHHCHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PPVLYFSALCNLVIGSGAFVLGGILEPMSESLNISVAAAGQAMTAYATATAVLAPLLII
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHH
LTARWTRKRAVQLALLLFATDCLLCALAPSLAVLLLGRVLMGAGAMFTAAASALAVSMVA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
PSQRGRALSITFLGMSISYAVGLPVDAWLGFEYGWRVPVWLSAAASWLIPATMASALAAS
CCCCCCEEEEEEEEEEEEHEECCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MARGEQAGRDEASAKPASPSAR
HHHCHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA