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The map label for this gene is cysL [H]

Identifier: 91789291

GI number: 91789291

Start: 3636706

End: 3637590

Strand: Direct

Name: cysL [H]

Synonym: Bpro_3436

Alternate gene names: 91789291

Gene position: 3636706-3637590 (Clockwise)

Preceding gene: 91789289

Following gene: 91789292

Centisome position: 69.93

GC content: 67.23

Gene sequence:

>885_bases
ATGCTCAATCTGGACCACCTGCGCACCTATGCGCTCATCATCGAAACCGGCAGCTTCTCGGGCGCGGCCGAAAGGCTGGG
TCTGTCGCAACCCGCCGTGAGCCTGCAGATCCGGCAGCTTGAGCAGCGGCTGCGGGTACGCCTGGTCGAGCGCGTGGCGC
GCCGAGCCACGCCCACGCCGGCGGGGCTGGATTTGCTGGAAGGCATTCAGCAGGTCAACACGGCGGTGGACGGCGTGATG
GCCGCCATGGCCTCGCACTCATCCAACGTCGAGGGCCGCGTGACACTGGGAACCGGCGCCACGGCGTGCCTGTATTTGCT
GCCGCAGATCCTGCGTTCGCTGAGGATCCGCTTTCCCAAGCTCAGCGTCGTGGTCAGTACCGGCAATACGGCCGACTTCG
TCAAGGCCGTGGAAGGCAATACGCTGGACCTGGCGCTGGTGACCCTGCCGGTCAAGCGCCGCGCGCTGGTCACGACGCCG
CTGCTGAACGATGAATTCGTGGCGATCGCCCCCAAGCGCGAGGCCGCGCTGCCTGCCCGGATCACGCCCGCCTTCCTCGC
CAAGCGCGAGCTTGTGCTGTTCGAGCCGGCGGCCAACACCCGCGTGATCATCGACCAGTGGTTTCGCGACGCGGGCCATG
CCCCCAAGCCCATCATGGAGCTGGGCAGCGTGGAAGCCATCAAGGAAATGGTGGCCGCGGGTCTGGGCTGCAGCATCCTG
CCGGCCATGGCCGTCACCGGCCCGGGCCAGCATCCCGAGCTGGTGGTCCACCCGCTTTACCCGCGGCTGCAAAGAACGCT
GGCGCTGGTCATGCGCGGCGACAAGCCGGTGAACAAGGCCTTGCACCAGGTCATTGCCGCGATCGGGCAACGAACGGGCA
ACTGA

Upstream 100 bases:

>100_bases
TGGGGGAGTCTGTGAAAAAAGCTGCATGCCGCTATGGTTGCGCAAGACCATGCATAAGTAAATATGTCAAATCTTATGGT
TTTCATTAGAATTTCTTTGC

Downstream 100 bases:

>100_bases
GCATCGCTGGCGCTGGCGCTGGCGCTGATCCTGGCCGGCATCGTCGGGCTGAAGATGGGCGGCGCCTGGCTTGAGACCGC
TGCTGAGCCGGTTTTCGTCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MLNLDHLRTYALIIETGSFSGAAERLGLSQPAVSLQIRQLEQRLRVRLVERVARRATPTPAGLDLLEGIQQVNTAVDGVM
AAMASHSSNVEGRVTLGTGATACLYLLPQILRSLRIRFPKLSVVVSTGNTADFVKAVEGNTLDLALVTLPVKRRALVTTP
LLNDEFVAIAPKREAALPARITPAFLAKRELVLFEPAANTRVIIDQWFRDAGHAPKPIMELGSVEAIKEMVAAGLGCSIL
PAMAVTGPGQHPELVVHPLYPRLQRTLALVMRGDKPVNKALHQVIAAIGQRTGN

Sequences:

>Translated_294_residues
MLNLDHLRTYALIIETGSFSGAAERLGLSQPAVSLQIRQLEQRLRVRLVERVARRATPTPAGLDLLEGIQQVNTAVDGVM
AAMASHSSNVEGRVTLGTGATACLYLLPQILRSLRIRFPKLSVVVSTGNTADFVKAVEGNTLDLALVTLPVKRRALVTTP
LLNDEFVAIAPKREAALPARITPAFLAKRELVLFEPAANTRVIIDQWFRDAGHAPKPIMELGSVEAIKEMVAAGLGCSIL
PAMAVTGPGQHPELVVHPLYPRLQRTLALVMRGDKPVNKALHQVIAAIGQRTGN
>Mature_294_residues
MLNLDHLRTYALIIETGSFSGAAERLGLSQPAVSLQIRQLEQRLRVRLVERVARRATPTPAGLDLLEGIQQVNTAVDGVM
AAMASHSSNVEGRVTLGTGATACLYLLPQILRSLRIRFPKLSVVVSTGNTADFVKAVEGNTLDLALVTLPVKRRALVTTP
LLNDEFVAIAPKREAALPARITPAFLAKRELVLFEPAANTRVIIDQWFRDAGHAPKPIMELGSVEAIKEMVAAGLGCSIL
PAMAVTGPGQHPELVVHPLYPRLQRTLALVMRGDKPVNKALHQVIAAIGQRTGN

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788748, Length=279, Percent_Identity=27.9569892473118, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790399, Length=293, Percent_Identity=30.0341296928328, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI145693105, Length=288, Percent_Identity=26.0416666666667, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1788481, Length=298, Percent_Identity=31.2080536912752, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082132, Length=290, Percent_Identity=27.5862068965517, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787530, Length=239, Percent_Identity=28.4518828451883, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787806, Length=251, Percent_Identity=25.4980079681275, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790262, Length=247, Percent_Identity=27.9352226720648, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787879, Length=245, Percent_Identity=29.3877551020408, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789204, Length=240, Percent_Identity=25.8333333333333, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI157672245, Length=272, Percent_Identity=26.4705882352941, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789506, Length=169, Percent_Identity=30.7692307692308, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788297, Length=252, Percent_Identity=25.3968253968254, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788887, Length=299, Percent_Identity=26.4214046822742, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI2367136, Length=292, Percent_Identity=23.972602739726, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790208, Length=253, Percent_Identity=27.2727272727273, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31693; Mature: 31693

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLDHLRTYALIIETGSFSGAAERLGLSQPAVSLQIRQLEQRLRVRLVERVARRATPTP
CCCHHHHHEEEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AGLDLLEGIQQVNTAVDGVMAAMASHSSNVEGRVTLGTGATACLYLLPQILRSLRIRFPK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCC
LSVVVSTGNTADFVKAVEGNTLDLALVTLPVKRRALVTTPLLNDEFVAIAPKREAALPAR
EEEEEECCCHHHHHHHHCCCEEEEEEEEECCCCCEEEECCCCCCCEEEECCCCCCCCCCC
ITPAFLAKRELVLFEPAANTRVIIDQWFRDAGHAPKPIMELGSVEAIKEMVAAGLGCSIL
CCHHHHHCCCEEEECCCCCCEEHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHCCCHHHH
PAMAVTGPGQHPELVVHPLYPRLQRTLALVMRGDKPVNKALHQVIAAIGQRTGN
HHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLNLDHLRTYALIIETGSFSGAAERLGLSQPAVSLQIRQLEQRLRVRLVERVARRATPTP
CCCHHHHHEEEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AGLDLLEGIQQVNTAVDGVMAAMASHSSNVEGRVTLGTGATACLYLLPQILRSLRIRFPK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCC
LSVVVSTGNTADFVKAVEGNTLDLALVTLPVKRRALVTTPLLNDEFVAIAPKREAALPAR
EEEEEECCCHHHHHHHHCCCEEEEEEEEECCCCCEEEECCCCCCCEEEECCCCCCCCCCC
ITPAFLAKRELVLFEPAANTRVIIDQWFRDAGHAPKPIMELGSVEAIKEMVAAGLGCSIL
CCHHHHHCCCEEEECCCCCCEEHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHCCCHHHH
PAMAVTGPGQHPELVVHPLYPRLQRTLALVMRGDKPVNKALHQVIAAIGQRTGN
HHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]