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The map label for this gene is gudD [H]

Identifier: 91789276

GI number: 91789276

Start: 3618476

End: 3619810

Strand: Direct

Name: gudD [H]

Synonym: Bpro_3421

Alternate gene names: 91789276

Gene position: 3618476-3619810 (Clockwise)

Preceding gene: 91789275

Following gene: 91789277

Centisome position: 69.58

GC content: 66.52

Gene sequence:

>1335_bases
ATGCCCGACACCTCCACACCCCTGATCACCGAGCTGCGCGTGATCCCGGTCGCCGGCCGCGACAGCATGCTGCTCAACCT
GAGCGGCGCGCACGGCCCCTTCTTCACACGCAACCTGTTGATCCTCACCGACAATTCCGGGCGCACCGGCGTGGGCGAGG
TGCCTGGCGGCGAGAAGATACGCCAGACCCTCGAAGACGCGCGCCCGCTGGTGGTGGGCCAGCCGATAGGCGCCCACCAG
CGCGTGCTGCAGCAGATGCAGCAGCAGTTTGCCGACCGCGACAGCGGCGGACGTGGACTGCAAACCTTCGACTTGCGCAC
CACCATCCACGCGGTGACCGCCGTTGAGTCGGCACTGCTGGATTTGCTGGGCCAGCACCTGGATGTGCCGGTCGCCGCAT
TGCTGGGCGAAGGCCAGCAGCGCGAGGCGGTCGAGATGCTGGGCTACCTGTTCTTTGTGGGCGACCGCAGCAAGACAGAC
CTGCCCTACAACAGCGAACCCAATGCCGACAACGCCTGGTCGCGGATACGCCACGAAGAAGCACTGACCCCCGAAACCAT
CGTGCGGCAGGCCGAAGCGGCGCAGGAGCGCTACGGCTTCAACGACTTCAAGCTCAAGGGCGGCGTGCTGCGCGGCGAGG
AAGAAATCGAGGCCGTGACGGCGCTGCACGAGCGCTTTCCGAACGCGCGGGTGACGCTGGACCCGAACGGAGGCTGGCTG
CTCAAGGACGCCATCCGCCTGATGCGCGACATGAAAAATGTCGTAGCCTACGCCGAAGACCCGTGCGGCGCCGAAGAAGG
CTTCTCGGGCCGCGAGGTCATGGCCGAGTTCCGCCGCGCCACCGGCCTGCCCACCGCCACCAACATGATCGCCACCGACT
GGCGGCAACTGCAACACGCCCTGTCGCTGCAGTCAGTGGACATTCCGCTGGCCGACCCGCATTTCTGGACCATGGCCGGC
TCGGTACGCGTGGCGCAGACTTGCCGCGACTGGGGTCTGACCTGGGGCTCGCATTCGAACAACCACTTCGACGTGTCGCT
CGCCATGTTCACCCATGTCGGCGCAGCCGCGCCGGGCCGCGTGACGGCCATCGACACGCACTGGATCTGGCAGGACGGCC
AGCGCCTCACCAAGGAGCCGCTGCAGATCGTGGGCGGGCAGGTAGCTGTGCCGAAGAAGCCCGGCCTCGGTGTCGAACTC
GACATGGCCGAGGTGGAAAAGGCGCACCAGCTCTACAAGCAGCACGGCCTGGGCGCGCGCGACGACGCTGTCGCCATGCA
AAGCCTGATACCCGGCTGGAAATTCAACCCCAAGCGTCCTGCTTTCGATCGCTGA

Upstream 100 bases:

>100_bases
AAAGTCATAGACACTGGAAAAATTACCGCCGATTGAGGCCGATTGAGGCTGATTCAACGATTCAAGATTCAAAGCGATTG
AGACCCCTTCCATGTCCAAC

Downstream 100 bases:

>100_bases
AAATGGACCGCTGAAAACTGGGTGCTGAAAATTGCCCCCCGAACCATACCGAAAGACGACTACCGATGTTTAAAAACCTC
ATCAACGGCGACTGGGTCGA

Product: D-glucarate dehydratase

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MPDTSTPLITELRVIPVAGRDSMLLNLSGAHGPFFTRNLLILTDNSGRTGVGEVPGGEKIRQTLEDARPLVVGQPIGAHQ
RVLQQMQQQFADRDSGGRGLQTFDLRTTIHAVTAVESALLDLLGQHLDVPVAALLGEGQQREAVEMLGYLFFVGDRSKTD
LPYNSEPNADNAWSRIRHEEALTPETIVRQAEAAQERYGFNDFKLKGGVLRGEEEIEAVTALHERFPNARVTLDPNGGWL
LKDAIRLMRDMKNVVAYAEDPCGAEEGFSGREVMAEFRRATGLPTATNMIATDWRQLQHALSLQSVDIPLADPHFWTMAG
SVRVAQTCRDWGLTWGSHSNNHFDVSLAMFTHVGAAAPGRVTAIDTHWIWQDGQRLTKEPLQIVGGQVAVPKKPGLGVEL
DMAEVEKAHQLYKQHGLGARDDAVAMQSLIPGWKFNPKRPAFDR

Sequences:

>Translated_444_residues
MPDTSTPLITELRVIPVAGRDSMLLNLSGAHGPFFTRNLLILTDNSGRTGVGEVPGGEKIRQTLEDARPLVVGQPIGAHQ
RVLQQMQQQFADRDSGGRGLQTFDLRTTIHAVTAVESALLDLLGQHLDVPVAALLGEGQQREAVEMLGYLFFVGDRSKTD
LPYNSEPNADNAWSRIRHEEALTPETIVRQAEAAQERYGFNDFKLKGGVLRGEEEIEAVTALHERFPNARVTLDPNGGWL
LKDAIRLMRDMKNVVAYAEDPCGAEEGFSGREVMAEFRRATGLPTATNMIATDWRQLQHALSLQSVDIPLADPHFWTMAG
SVRVAQTCRDWGLTWGSHSNNHFDVSLAMFTHVGAAAPGRVTAIDTHWIWQDGQRLTKEPLQIVGGQVAVPKKPGLGVEL
DMAEVEKAHQLYKQHGLGARDDAVAMQSLIPGWKFNPKRPAFDR
>Mature_443_residues
PDTSTPLITELRVIPVAGRDSMLLNLSGAHGPFFTRNLLILTDNSGRTGVGEVPGGEKIRQTLEDARPLVVGQPIGAHQR
VLQQMQQQFADRDSGGRGLQTFDLRTTIHAVTAVESALLDLLGQHLDVPVAALLGEGQQREAVEMLGYLFFVGDRSKTDL
PYNSEPNADNAWSRIRHEEALTPETIVRQAEAAQERYGFNDFKLKGGVLRGEEEIEAVTALHERFPNARVTLDPNGGWLL
KDAIRLMRDMKNVVAYAEDPCGAEEGFSGREVMAEFRRATGLPTATNMIATDWRQLQHALSLQSVDIPLADPHFWTMAGS
VRVAQTCRDWGLTWGSHSNNHFDVSLAMFTHVGAAAPGRVTAIDTHWIWQDGQRLTKEPLQIVGGQVAVPKKPGLGVELD
MAEVEKAHQLYKQHGLGARDDAVAMQSLIPGWKFNPKRPAFDR

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc). Also acts on L-idarate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=441, Percent_Identity=72.7891156462585, Blast_Score=689, Evalue=0.0,
Organism=Escherichia coli, GI1789151, Length=447, Percent_Identity=63.3109619686801, Blast_Score=576, Evalue=1e-166,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 48951; Mature: 48820

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTSTPLITELRVIPVAGRDSMLLNLSGAHGPFFTRNLLILTDNSGRTGVGEVPGGEKI
CCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCEECEEEEEECCCCCCCCCCCCCHHHH
RQTLEDARPLVVGQPIGAHQRVLQQMQQQFADRDSGGRGLQTFDLRTTIHAVTAVESALL
HHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHH
DLLGQHLDVPVAALLGEGQQREAVEMLGYLFFVGDRSKTDLPYNSEPNADNAWSRIRHEE
HHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHH
ALTPETIVRQAEAAQERYGFNDFKLKGGVLRGEEEIEAVTALHERFPNARVTLDPNGGWL
CCCHHHHHHHHHHHHHHCCCCCEEECCCEECCHHHHHHHHHHHHHCCCCEEEECCCCCEE
LKDAIRLMRDMKNVVAYAEDPCGAEEGFSGREVMAEFRRATGLPTATNMIATDWRQLQHA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LSLQSVDIPLADPHFWTMAGSVRVAQTCRDWGLTWGSHSNNHFDVSLAMFTHVGAAAPGR
HHHHHCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHCCCCCCC
VTAIDTHWIWQDGQRLTKEPLQIVGGQVAVPKKPGLGVELDMAEVEKAHQLYKQHGLGAR
EEEEEEEEEECCCHHHHHHHHHHHCCEEECCCCCCCCEEECHHHHHHHHHHHHHHCCCCC
DDAVAMQSLIPGWKFNPKRPAFDR
HHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PDTSTPLITELRVIPVAGRDSMLLNLSGAHGPFFTRNLLILTDNSGRTGVGEVPGGEKI
CCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCEECEEEEEECCCCCCCCCCCCCHHHH
RQTLEDARPLVVGQPIGAHQRVLQQMQQQFADRDSGGRGLQTFDLRTTIHAVTAVESALL
HHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHH
DLLGQHLDVPVAALLGEGQQREAVEMLGYLFFVGDRSKTDLPYNSEPNADNAWSRIRHEE
HHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHH
ALTPETIVRQAEAAQERYGFNDFKLKGGVLRGEEEIEAVTALHERFPNARVTLDPNGGWL
CCCHHHHHHHHHHHHHHCCCCCEEECCCEECCHHHHHHHHHHHHHCCCCEEEECCCCCEE
LKDAIRLMRDMKNVVAYAEDPCGAEEGFSGREVMAEFRRATGLPTATNMIATDWRQLQHA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LSLQSVDIPLADPHFWTMAGSVRVAQTCRDWGLTWGSHSNNHFDVSLAMFTHVGAAAPGR
HHHHHCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHCCCCCCC
VTAIDTHWIWQDGQRLTKEPLQIVGGQVAVPKKPGLGVELDMAEVEKAHQLYKQHGLGAR
EEEEEEEEEECCCHHHHHHHHHHHCCEEECCCCCCCCEEECHHHHHHHHHHHHHHCCCCC
DDAVAMQSLIPGWKFNPKRPAFDR
HHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]