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The map label for this gene is livM [H]

Identifier: 91789238

GI number: 91789238

Start: 3575274

End: 3576254

Strand: Reverse

Name: livM [H]

Synonym: Bpro_3382

Alternate gene names: 91789238

Gene position: 3576254-3575274 (Counterclockwise)

Preceding gene: 91789239

Following gene: 91789237

Centisome position: 68.77

GC content: 66.36

Gene sequence:

>981_bases
ATGAACAACAAACCACTCCTGCCGGTCCGGCAGTCCCTGCTGAATGCCTCGCGCTGGAAACCCTGGGAGCCGGTGCTGTG
GCTGCTGGCGTTTGCCTCGCCGCTGCTGCTGTCCAGCCATGCGCTGATCATCAATGAAATCGCCATCGTCGCGCTGTTTG
CCATCTCGCTGGACCTGATCCTGGGCTTCACCGGCATTGTGTCGCTGGGGCACGCGGCGTTTTTTGGCCTGGGCGCTTAT
GCCGCCGCGCTGTTTGCCAAACTGGTCATGCCCGATCCGCTGGTCGGGCTGGCGGTGGCGATTGCGGTGTCCACGCTGAT
GGGCGCCGCCTGCAGCCTGACCATCATGCGCGGCAGCGACCTGACGCGCCTGATGGTCACGCTGGGCATGGGCCTGATCC
TGCTGGAGCTGGCCAACAAGCTTGACTGGCTCACCGGCGGGGCCGACGGCCTGCAGGGCGTGCTGATGGGGCCGCTGCTG
GGCCGCTTCGAGTTTGACCTGTCGGGCCGCACCGCCGCGTATTACTCGCTGTGCGTCGGCCTGATTCTCTTTTTGCTCGC
CCGGCGCCTGGTGCATTCGCCGTTTGGCGCCACCCTGATGGCGATTCGCGACAACCGGCTGCGCGCGATGGCCATCGGCA
TTCCGGTGCATGCGCGCCTGGCCGTGATCTACACGGTGTCGGCCGGGGTTGCCGGTGCGGCCGGCGGCCTGCTGGCGCAG
ACCACCGGCTTTGCCTCGCTGGACGTGTTTGACTTTCACCGCTCGGCCGACGTGATGCTGGTGCTGGTGGTGGGCGGCGT
CGGCTGGCTGTACGGCGGTATTGCCGGTGCGGTGGTGTTCAAGCTGATGCAGGACATCCTGTCGGCCATCACCCCGCAGT
ACTGGACGTTCTGGATTGGCCTGTTCCTTGTGGTGCTGGTGCTGGTCGGGCGCGAGCGCCTGCTGCGGCCCTGGACCTGG
TTCAGGAGGGCCAAGGCATGA

Upstream 100 bases:

>100_bases
ACTACATCCCCAAACTCGGCGCCTTCATCGTCTACAGCCTGATGATCGCCATCCTGATCTGGCGTCCGCAAGGCCTGTTT
GTGCGCAAAGGCGGCAAGTG

Downstream 100 bases:

>100_bases
GAACCGATCAGCCCCCTCCGACCGGTCAGGTCGATCAGACCGTGCTGTCGGCCAGCGGCCTGGTGATGCGCTTTGGCGGC
ATCACGGCCACCAACAACGT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MNNKPLLPVRQSLLNASRWKPWEPVLWLLAFASPLLLSSHALIINEIAIVALFAISLDLILGFTGIVSLGHAAFFGLGAY
AAALFAKLVMPDPLVGLAVAIAVSTLMGAACSLTIMRGSDLTRLMVTLGMGLILLELANKLDWLTGGADGLQGVLMGPLL
GRFEFDLSGRTAAYYSLCVGLILFLLARRLVHSPFGATLMAIRDNRLRAMAIGIPVHARLAVIYTVSAGVAGAAGGLLAQ
TTGFASLDVFDFHRSADVMLVLVVGGVGWLYGGIAGAVVFKLMQDILSAITPQYWTFWIGLFLVVLVLVGRERLLRPWTW
FRRAKA

Sequences:

>Translated_326_residues
MNNKPLLPVRQSLLNASRWKPWEPVLWLLAFASPLLLSSHALIINEIAIVALFAISLDLILGFTGIVSLGHAAFFGLGAY
AAALFAKLVMPDPLVGLAVAIAVSTLMGAACSLTIMRGSDLTRLMVTLGMGLILLELANKLDWLTGGADGLQGVLMGPLL
GRFEFDLSGRTAAYYSLCVGLILFLLARRLVHSPFGATLMAIRDNRLRAMAIGIPVHARLAVIYTVSAGVAGAAGGLLAQ
TTGFASLDVFDFHRSADVMLVLVVGGVGWLYGGIAGAVVFKLMQDILSAITPQYWTFWIGLFLVVLVLVGRERLLRPWTW
FRRAKA
>Mature_326_residues
MNNKPLLPVRQSLLNASRWKPWEPVLWLLAFASPLLLSSHALIINEIAIVALFAISLDLILGFTGIVSLGHAAFFGLGAY
AAALFAKLVMPDPLVGLAVAIAVSTLMGAACSLTIMRGSDLTRLMVTLGMGLILLELANKLDWLTGGADGLQGVLMGPLL
GRFEFDLSGRTAAYYSLCVGLILFLLARRLVHSPFGATLMAIRDNRLRAMAIGIPVHARLAVIYTVSAGVAGAAGGLLAQ
TTGFASLDVFDFHRSADVMLVLVVGGVGWLYGGIAGAVVFKLMQDILSAITPQYWTFWIGLFLVVLVLVGRERLLRPWTW
FRRAKA

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=315, Percent_Identity=26.031746031746, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 34972; Mature: 34972

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKPLLPVRQSLLNASRWKPWEPVLWLLAFASPLLLSSHALIINEIAIVALFAISLDLI
CCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LGFTGIVSLGHAAFFGLGAYAAALFAKLVMPDPLVGLAVAIAVSTLMGAACSLTIMRGSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEECCCC
LTRLMVTLGMGLILLELANKLDWLTGGADGLQGVLMGPLLGRFEFDLSGRTAAYYSLCVG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCEEECCCCCHHHHHHHHHH
LILFLLARRLVHSPFGATLMAIRDNRLRAMAIGIPVHARLAVIYTVSAGVAGAAGGLLAQ
HHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCHHHHEEEEEEECCCCCCCCCCHHHH
TTGFASLDVFDFHRSADVMLVLVVGGVGWLYGGIAGAVVFKLMQDILSAITPQYWTFWIG
CCCCCEEEHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LFLVVLVLVGRERLLRPWTWFRRAKA
HHHHHHHHHCHHHHHCHHHHHHHCCC
>Mature Secondary Structure
MNNKPLLPVRQSLLNASRWKPWEPVLWLLAFASPLLLSSHALIINEIAIVALFAISLDLI
CCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LGFTGIVSLGHAAFFGLGAYAAALFAKLVMPDPLVGLAVAIAVSTLMGAACSLTIMRGSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEECCCC
LTRLMVTLGMGLILLELANKLDWLTGGADGLQGVLMGPLLGRFEFDLSGRTAAYYSLCVG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCEEECCCCCHHHHHHHHHH
LILFLLARRLVHSPFGATLMAIRDNRLRAMAIGIPVHARLAVIYTVSAGVAGAAGGLLAQ
HHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCHHHHEEEEEEECCCCCCCCCCHHHH
TTGFASLDVFDFHRSADVMLVLVVGGVGWLYGGIAGAVVFKLMQDILSAITPQYWTFWIG
CCCCCEEEHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LFLVVLVLVGRERLLRPWTWFRRAKA
HHHHHHHHHCHHHHHCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]