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The map label for this gene is acsA [H]

Identifier: 91789214

GI number: 91789214

Start: 3553874

End: 3555940

Strand: Reverse

Name: acsA [H]

Synonym: Bpro_3358

Alternate gene names: 91789214

Gene position: 3555940-3553874 (Counterclockwise)

Preceding gene: 91789219

Following gene: 91789213

Centisome position: 68.38

GC content: 63.47

Gene sequence:

>2067_bases
ATGTCAAAAGACAAGTTGCCGCGCCACGCCACCGAGCAGCAGAGTGCCGATATCTCCGAGATCTCCGAGGCCCAGATCGC
GGTGCATTGGCCGGAGGAGGGCTACTTCTCGCCGTCCAACCCCTTCATCGCGCAGGCCAATTTGACGGACGCGGGCATCT
TCGCGCGTTTTGCCCTCGACAAGTTTCCCGAGTGCTTCAAGGAGTTCGCGGACCTGCTGGACTGGTACAAGTACTGGGAG
ACGACGCTCGACACCAGCAATCCCCCGTTCTGGCGCTGGTTCGTCGGCGGCAAGATCAACGCTTGCTACAACTGCGTGGA
CCGCCACCTTGGCCAGCACAAGAACAAGACGGCGATCCATTTTGTGCCCGAGCCGGAGGACGAGGCGCCACAGCACCTCA
CCTACCAGGAGCTTTTCGTGCGCGTCAACGAGTTCGCGGCCTTGTTGCGTGACTTCTGCGGCCTGAAGGCGGGCGACCGG
GTGACCTTGCATATGCCGATGGTGGCGGAGCTGCCCATCACCATGCTTGCCTGCGCGCGGCTCGGCGTGATTCACTCGCA
GGTGTTCAGCGGATTCAGCGGCAAGGCCTGCGCCGACCGCGTCGCGGATTCCGAGAGCCGCGTGTTGATCACCATGGACG
CCTATCACCGCGCCGGCAATCTGCTGGACCATAAGGAGAAAGCCGACATCGCGGTCATGGAGGCCGCCAGCCACGGCCAA
CAGGTCGACAAGGTGCTGATCTGGCAGCGCTACCCGGGCAAAAACTCCAGCCCCACGAAGCTCGTGGAAGGACGCGACTT
CGTCATCAACGACGTCCTCAAGCAGTTCCGTGGCAAGCGTGTCGAGCCCGTGCAGATGCCGGCAGAGGCGCCGCTTTTCC
TGATGTACACGAGCGGCACGACGGGGCGGCCCAAAGGCTGCCAGCACGGCATCGGCGGCTACCTCGCCTACGTCACCTGG
ACATCGAAGTTCATCCAGGACATCCACCCCGAGGACGTGTACTGGTGCATGGCCGACATCGGCTGGATCACAGGCCATTC
CTACATCGTCTACGGCCCGCTGGCGCTCGCCGCTTCTTCGGTGGTGTACGAAGGTGTGCCAACCTATCCGGACGCCGGAC
GGCCCTGGCGTATCGCTGAAAACCTCGACGTCAATATCTTCCACACGTCGCCCACCGCGATCCGCGCGCTACGGCGCGAC
GGACCCGACGAGCCCGCCAAGTACAACCACCACTTCAAACACATGACGACCGTGGGCGAGCCGATCGAGCCCGAAGTCTG
GAAGTGGTACCACCGCATGGTCGGCAAGGGCGAAGCAGTGATCGTGGACACCTGGTGGCAGACCGAAAACGGCGGATTCC
TTTGCAGCACGGTGCCGGCGATCCACAAGATGAAGCCCGGCAGCACGGGCCCCGCGATTCCAGGCATTCATCCGGTGATC
TACGACGACGAGGGCAAGGAGCTTGTGAGCGGTTCGGGCAAGGCGGGCAACATCTGCATCCGCAACCCCTGGCCCGGTGG
TTTCCAGACCATCTGGAAAGACCCCGAGCGCTACGTGCGCCAGTACTACGCGCGCTATTGCGCAGATCCCAAGAGCAAAG
ACTGGCGCGACTGGCCCTACATGGCGGGTGATGGCGCGGTGCAGGCGGCCGACGGTTACTACCGGATCCTCGGCCGCATC
GACGACGTGATCAACGTCGCCGGCCATCGCCTGGGCACCAAGGAAATCGAATCGGCCGCGCTGCTCGTGCCCGAGGTCGT
CGAAGCGGCCGTGGTTCCAGTCGCCGACCCGATCAAGGGCAAGGTGCCCGACCTCTATGTTGCGTTGAAGCCGGGACTGG
CGCCTTCCGAGGAGATCGCGAACAGGGTCTCGGCATCGGTCGTGTCCGAGATCGGCGCCATCGCGCGGCCTCGCCGAGTG
ATTGTCGTTCCCGATATGCCCAAGACCCGCTCGGGAAAGATCATGCGCCGCGTGCTCGCGGCGATCTCGAATCACCTGGA
TCCTGGCGACGTGTCCACCCTCGCCAATCCGGAGGTCGTCGACAAGATCAAGGAGCTGGCCAAGTAG

Upstream 100 bases:

>100_bases
ATTATTGCGACAAACGCAGTTCGCTCGTTGCGCTTGACGGCGGCACCAAGGCGCGCCGAGTGCCTGCATCGGAGTCACGG
AAAATCAAGGAGGAACGGTC

Downstream 100 bases:

>100_bases
CGCCAATGCGCGCACGACGCGGGATCCCCGCGAGCTCTCGCGCAGCCAGGGGCGGGGTTGCCACGCCCTTGCGCAAAGCT
GACTTTTGCCATGAGGGTCA

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Acetate--CoA ligase; Acyl-activating enzyme [H]

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MSKDKLPRHATEQQSADISEISEAQIAVHWPEEGYFSPSNPFIAQANLTDAGIFARFALDKFPECFKEFADLLDWYKYWE
TTLDTSNPPFWRWFVGGKINACYNCVDRHLGQHKNKTAIHFVPEPEDEAPQHLTYQELFVRVNEFAALLRDFCGLKAGDR
VTLHMPMVAELPITMLACARLGVIHSQVFSGFSGKACADRVADSESRVLITMDAYHRAGNLLDHKEKADIAVMEAASHGQ
QVDKVLIWQRYPGKNSSPTKLVEGRDFVINDVLKQFRGKRVEPVQMPAEAPLFLMYTSGTTGRPKGCQHGIGGYLAYVTW
TSKFIQDIHPEDVYWCMADIGWITGHSYIVYGPLALAASSVVYEGVPTYPDAGRPWRIAENLDVNIFHTSPTAIRALRRD
GPDEPAKYNHHFKHMTTVGEPIEPEVWKWYHRMVGKGEAVIVDTWWQTENGGFLCSTVPAIHKMKPGSTGPAIPGIHPVI
YDDEGKELVSGSGKAGNICIRNPWPGGFQTIWKDPERYVRQYYARYCADPKSKDWRDWPYMAGDGAVQAADGYYRILGRI
DDVINVAGHRLGTKEIESAALLVPEVVEAAVVPVADPIKGKVPDLYVALKPGLAPSEEIANRVSASVVSEIGAIARPRRV
IVVPDMPKTRSGKIMRRVLAAISNHLDPGDVSTLANPEVVDKIKELAK

Sequences:

>Translated_688_residues
MSKDKLPRHATEQQSADISEISEAQIAVHWPEEGYFSPSNPFIAQANLTDAGIFARFALDKFPECFKEFADLLDWYKYWE
TTLDTSNPPFWRWFVGGKINACYNCVDRHLGQHKNKTAIHFVPEPEDEAPQHLTYQELFVRVNEFAALLRDFCGLKAGDR
VTLHMPMVAELPITMLACARLGVIHSQVFSGFSGKACADRVADSESRVLITMDAYHRAGNLLDHKEKADIAVMEAASHGQ
QVDKVLIWQRYPGKNSSPTKLVEGRDFVINDVLKQFRGKRVEPVQMPAEAPLFLMYTSGTTGRPKGCQHGIGGYLAYVTW
TSKFIQDIHPEDVYWCMADIGWITGHSYIVYGPLALAASSVVYEGVPTYPDAGRPWRIAENLDVNIFHTSPTAIRALRRD
GPDEPAKYNHHFKHMTTVGEPIEPEVWKWYHRMVGKGEAVIVDTWWQTENGGFLCSTVPAIHKMKPGSTGPAIPGIHPVI
YDDEGKELVSGSGKAGNICIRNPWPGGFQTIWKDPERYVRQYYARYCADPKSKDWRDWPYMAGDGAVQAADGYYRILGRI
DDVINVAGHRLGTKEIESAALLVPEVVEAAVVPVADPIKGKVPDLYVALKPGLAPSEEIANRVSASVVSEIGAIARPRRV
IVVPDMPKTRSGKIMRRVLAAISNHLDPGDVSTLANPEVVDKIKELAK
>Mature_687_residues
SKDKLPRHATEQQSADISEISEAQIAVHWPEEGYFSPSNPFIAQANLTDAGIFARFALDKFPECFKEFADLLDWYKYWET
TLDTSNPPFWRWFVGGKINACYNCVDRHLGQHKNKTAIHFVPEPEDEAPQHLTYQELFVRVNEFAALLRDFCGLKAGDRV
TLHMPMVAELPITMLACARLGVIHSQVFSGFSGKACADRVADSESRVLITMDAYHRAGNLLDHKEKADIAVMEAASHGQQ
VDKVLIWQRYPGKNSSPTKLVEGRDFVINDVLKQFRGKRVEPVQMPAEAPLFLMYTSGTTGRPKGCQHGIGGYLAYVTWT
SKFIQDIHPEDVYWCMADIGWITGHSYIVYGPLALAASSVVYEGVPTYPDAGRPWRIAENLDVNIFHTSPTAIRALRRDG
PDEPAKYNHHFKHMTTVGEPIEPEVWKWYHRMVGKGEAVIVDTWWQTENGGFLCSTVPAIHKMKPGSTGPAIPGIHPVIY
DDEGKELVSGSGKAGNICIRNPWPGGFQTIWKDPERYVRQYYARYCADPKSKDWRDWPYMAGDGAVQAADGYYRILGRID
DVINVAGHRLGTKEIESAALLVPEVVEAAVVPVADPIKGKVPDLYVALKPGLAPSEEIANRVSASVVSEIGAIARPRRVI
VVPDMPKTRSGKIMRRVLAAISNHLDPGDVSTLANPEVVDKIKELAK

Specific function: Enables The Cell To Use Acetate During Aerobic Growth To Generate Energy Via The Tca Cycle, And Biosynthetic Compounds Via The Glyoxylate Shunt. Acetylates Chey, The Response Regulator Involved In Flagellar Movement And Chemotaxis. [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI8923896, Length=622, Percent_Identity=40.8360128617363, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI253970489, Length=634, Percent_Identity=40.6940063091483, Blast_Score=445, Evalue=1e-125,
Organism=Homo sapiens, GI28416953, Length=600, Percent_Identity=38.3333333333333, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI13375727, Length=637, Percent_Identity=34.850863422292, Blast_Score=354, Evalue=2e-97,
Organism=Homo sapiens, GI42544132, Length=535, Percent_Identity=26.5420560747664, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI38505220, Length=538, Percent_Identity=26.7657992565056, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI115511026, Length=534, Percent_Identity=26.2172284644195, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI122937307, Length=530, Percent_Identity=26.6037735849057, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI58082049, Length=537, Percent_Identity=25.8845437616387, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI31982927, Length=601, Percent_Identity=21.9633943427621, Blast_Score=135, Evalue=2e-31,
Organism=Homo sapiens, GI157311624, Length=537, Percent_Identity=24.9534450651769, Blast_Score=129, Evalue=9e-30,
Organism=Homo sapiens, GI157311622, Length=537, Percent_Identity=24.9534450651769, Blast_Score=129, Evalue=9e-30,
Organism=Escherichia coli, GI1790505, Length=631, Percent_Identity=41.5213946117274, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI1786529, Length=643, Percent_Identity=36.2363919129082, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71982997, Length=604, Percent_Identity=39.4039735099338, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71983001, Length=604, Percent_Identity=39.4039735099338, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17569507, Length=692, Percent_Identity=22.6878612716763, Blast_Score=125, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6323182, Length=607, Percent_Identity=41.0214168039539, Blast_Score=439, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6319264, Length=641, Percent_Identity=40.0936037441498, Blast_Score=427, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6319699, Length=402, Percent_Identity=24.3781094527363, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24667955, Length=674, Percent_Identity=36.646884272997, Blast_Score=429, Evalue=1e-120,
Organism=Drosophila melanogaster, GI62472339, Length=566, Percent_Identity=39.3992932862191, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24667959, Length=528, Percent_Identity=39.3939393939394, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI21355225, Length=618, Percent_Identity=34.6278317152104, Blast_Score=332, Evalue=8e-91,
Organism=Drosophila melanogaster, GI24581924, Length=538, Percent_Identity=22.6765799256506, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648260, Length=380, Percent_Identity=22.6315789473684, Blast_Score=71, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011904
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.1 [H]

Molecular weight: Translated: 76671; Mature: 76540

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDKLPRHATEQQSADISEISEAQIAVHWPEEGYFSPSNPFIAQANLTDAGIFARFALD
CCCCCCCCCCCHHHCCCHHHHCCCEEEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
KFPECFKEFADLLDWYKYWETTLDTSNPPFWRWFVGGKINACYNCVDRHLGQHKNKTAIH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCEEE
FVPEPEDEAPQHLTYQELFVRVNEFAALLRDFCGLKAGDRVTLHMPMVAELPITMLACAR
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHH
LGVIHSQVFSGFSGKACADRVADSESRVLITMDAYHRAGNLLDHKEKADIAVMEAASHGQ
HHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEEECHHHCCCCCCCCCCCCEEEEHHHHHHH
QVDKVLIWQRYPGKNSSPTKLVEGRDFVINDVLKQFRGKRVEPVQMPAEAPLFLMYTSGT
HHHHEEEEEECCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCC
TGRPKGCQHGIGGYLAYVTWTSKFIQDIHPEDVYWCMADIGWITGHSYIVYGPLALAASS
CCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHH
VVYEGVPTYPDAGRPWRIAENLDVNIFHTSPTAIRALRRDGPDEPAKYNHHFKHMTTVGE
HHHCCCCCCCCCCCCEEEECCCEEEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCC
PIEPEVWKWYHRMVGKGEAVIVDTWWQTENGGFLCSTVPAIHKMKPGSTGPAIPGIHPVI
CCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCEEEECCHHHHHCCCCCCCCCCCCCCCEE
YDDEGKELVSGSGKAGNICIRNPWPGGFQTIWKDPERYVRQYYARYCADPKSKDWRDWPY
ECCCCCHHHCCCCCCCCEEEECCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCE
MAGDGAVQAADGYYRILGRIDDVINVAGHRLGTKEIESAALLVPEVVEAAVVPVADPIKG
ECCCCCEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KVPDLYVALKPGLAPSEEIANRVSASVVSEIGAIARPRRVIVVPDMPKTRSGKIMRRVLA
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
AISNHLDPGDVSTLANPEVVDKIKELAK
HHHCCCCCCCHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SKDKLPRHATEQQSADISEISEAQIAVHWPEEGYFSPSNPFIAQANLTDAGIFARFALD
CCCCCCCCCCHHHCCCHHHHCCCEEEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
KFPECFKEFADLLDWYKYWETTLDTSNPPFWRWFVGGKINACYNCVDRHLGQHKNKTAIH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCEEE
FVPEPEDEAPQHLTYQELFVRVNEFAALLRDFCGLKAGDRVTLHMPMVAELPITMLACAR
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHH
LGVIHSQVFSGFSGKACADRVADSESRVLITMDAYHRAGNLLDHKEKADIAVMEAASHGQ
HHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEEECHHHCCCCCCCCCCCCEEEEHHHHHHH
QVDKVLIWQRYPGKNSSPTKLVEGRDFVINDVLKQFRGKRVEPVQMPAEAPLFLMYTSGT
HHHHEEEEEECCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCC
TGRPKGCQHGIGGYLAYVTWTSKFIQDIHPEDVYWCMADIGWITGHSYIVYGPLALAASS
CCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHH
VVYEGVPTYPDAGRPWRIAENLDVNIFHTSPTAIRALRRDGPDEPAKYNHHFKHMTTVGE
HHHCCCCCCCCCCCCEEEECCCEEEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCC
PIEPEVWKWYHRMVGKGEAVIVDTWWQTENGGFLCSTVPAIHKMKPGSTGPAIPGIHPVI
CCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCEEEECCHHHHHCCCCCCCCCCCCCCCEE
YDDEGKELVSGSGKAGNICIRNPWPGGFQTIWKDPERYVRQYYARYCADPKSKDWRDWPY
ECCCCCHHHCCCCCCCCEEEECCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCE
MAGDGAVQAADGYYRILGRIDDVINVAGHRLGTKEIESAALLVPEVVEAAVVPVADPIKG
ECCCCCEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KVPDLYVALKPGLAPSEEIANRVSASVVSEIGAIARPRRVIVVPDMPKTRSGKIMRRVLA
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
AISNHLDPGDVSTLANPEVVDKIKELAK
HHHCCCCCCCHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500908 [H]