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The map label for this gene is livJ [H]

Identifier: 91789192

GI number: 91789192

Start: 3530165

End: 3531343

Strand: Reverse

Name: livJ [H]

Synonym: Bpro_3336

Alternate gene names: 91789192

Gene position: 3531343-3530165 (Counterclockwise)

Preceding gene: 91789197

Following gene: 91789191

Centisome position: 67.91

GC content: 55.56

Gene sequence:

>1179_bases
ATGCGTAGGCGGAACACGATATTGGCAGGAACGGCGTTGTTGACGACATGTCTGAGTCTGGCTCTCCTGGCCAGTCCAGT
CATGGCTAAAGAAGTGGTGAAAATCGCCTTTGTCGGTCCGTTGACCGGTGGGGTCTCCAATATGGGTCTGGGGGGGCGCA
ATTCAGCAGACTTGGCAGTGCGCCTGCGCAATCAGGACCCCAAATCCAAATACACCTATGAATTGGTGGCGCAGGACGAC
GAGTGCAAGCCCAATGTGGGTGTGCAGGTGGCGACCAAGGTGGCCGCAGACAAAAATATCGTGGCGGGCGTCACACACTA
TTGCTCGGCAGTCGCCATGGCCACGGCGGGCGTGTACAACCGTTTTGGCATGCCGGCTGTGGTGTGGGGTGCGGTTCTGC
CCGAGGTCACCTACGGCAACAACTTCCAGGAAGTTCATCGTGTCAACGGCACGATGATCAATCAGAACGAGGTCGCCTAC
AAGTTCATGGCCGGACTGGGCTACAAAAAATGGGCCATTATTCATGACACAACGGACTACGGCAAAGGACACAACAAATA
CTTCAGCGAATTCCTGAAAAAAGGTGGCGGTACGGTCCTGGGCACCTTCGGTGTGACGGCGGACCAACAGGATTTCACCA
CCGAACTGACCAAGATTCGTGAGTTGAATCCCGACGTCATTTACTTCGGCGGACTGACGCCGCTCGGGGTACGGATTCGG
ACGCAGATGGAGAAGCTGGGCATCAAGGCTCAATTCCAGGGTACCTCCGGGATAAAGTCCGATGCCTATATTCAGGGTGC
TGGCAAGGAGCTGGCTGAAGGCACGCTTTCGTTTATCGAAGGGGCGCCATGGGAGAAGCTGCCAGGCGGGCTTTTCTTCA
TCAGCAAATACGCGCAGCAAAAATACGGTGAATCGGCAGAGGCTTATGGTCCCTTTGCATTTGCCGCAGCCAATCTGATC
ATGGATGCCGTCGAGAAGGTCGGGCCGGATCGCAAAAAAGTGCGTGACGTTCTGAACAGGACCAAGGATGCGGACACCAT
TGTTGGCAAGGTGACCTTTGACGATCATCGCCAGAACATCGTGCCGCTCATCACCAAGTACGTGGTGGAAAACGGTGCAT
GGGTGGTTTGGGAAGACAGCAGCTACGGCAAGGGCAAGCACAAGCTGGCCGGTTTGTAA

Upstream 100 bases:

>100_bases
CTTGCGAGTATTCAAGTGACTTGAATTCTATTCAATAGACTGGCAACCAGAATCATGTCACTTAATTCAAGTTCATCAAT
TCACCCGAAAGGAAGCGACT

Downstream 100 bases:

>100_bases
TCCCCAAGATGCCCTCCTGCACGCCATCGGCGACGCGGGAGGGTAACCCTCAATCGTTTCAGGAAACCTGATGAGTGAAG
TTAGTCAATACATCTTCAAT

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-BP [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MRRRNTILAGTALLTTCLSLALLASPVMAKEVVKIAFVGPLTGGVSNMGLGGRNSADLAVRLRNQDPKSKYTYELVAQDD
ECKPNVGVQVATKVAADKNIVAGVTHYCSAVAMATAGVYNRFGMPAVVWGAVLPEVTYGNNFQEVHRVNGTMINQNEVAY
KFMAGLGYKKWAIIHDTTDYGKGHNKYFSEFLKKGGGTVLGTFGVTADQQDFTTELTKIRELNPDVIYFGGLTPLGVRIR
TQMEKLGIKAQFQGTSGIKSDAYIQGAGKELAEGTLSFIEGAPWEKLPGGLFFISKYAQQKYGESAEAYGPFAFAAANLI
MDAVEKVGPDRKKVRDVLNRTKDADTIVGKVTFDDHRQNIVPLITKYVVENGAWVVWEDSSYGKGKHKLAGL

Sequences:

>Translated_392_residues
MRRRNTILAGTALLTTCLSLALLASPVMAKEVVKIAFVGPLTGGVSNMGLGGRNSADLAVRLRNQDPKSKYTYELVAQDD
ECKPNVGVQVATKVAADKNIVAGVTHYCSAVAMATAGVYNRFGMPAVVWGAVLPEVTYGNNFQEVHRVNGTMINQNEVAY
KFMAGLGYKKWAIIHDTTDYGKGHNKYFSEFLKKGGGTVLGTFGVTADQQDFTTELTKIRELNPDVIYFGGLTPLGVRIR
TQMEKLGIKAQFQGTSGIKSDAYIQGAGKELAEGTLSFIEGAPWEKLPGGLFFISKYAQQKYGESAEAYGPFAFAAANLI
MDAVEKVGPDRKKVRDVLNRTKDADTIVGKVTFDDHRQNIVPLITKYVVENGAWVVWEDSSYGKGKHKLAGL
>Mature_392_residues
MRRRNTILAGTALLTTCLSLALLASPVMAKEVVKIAFVGPLTGGVSNMGLGGRNSADLAVRLRNQDPKSKYTYELVAQDD
ECKPNVGVQVATKVAADKNIVAGVTHYCSAVAMATAGVYNRFGMPAVVWGAVLPEVTYGNNFQEVHRVNGTMINQNEVAY
KFMAGLGYKKWAIIHDTTDYGKGHNKYFSEFLKKGGGTVLGTFGVTADQQDFTTELTKIRELNPDVIYFGGLTPLGVRIR
TQMEKLGIKAQFQGTSGIKSDAYIQGAGKELAEGTLSFIEGAPWEKLPGGLFFISKYAQQKYGESAEAYGPFAFAAANLI
MDAVEKVGPDRKKVRDVLNRTKDADTIVGKVTFDDHRQNIVPLITKYVVENGAWVVWEDSSYGKGKHKLAGL

Specific function: This protein is a component of the leucine, isoleucine, valine, (threonine) transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=350, Percent_Identity=28.5714285714286, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1789867, Length=361, Percent_Identity=26.8698060941828, Blast_Score=111, Evalue=8e-26,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42568; Mature: 42568

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRRNTILAGTALLTTCLSLALLASPVMAKEVVKIAFVGPLTGGVSNMGLGGRNSADLAV
CCCCCEEEEHHHHHHHHHHHHHHHCHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCEEEE
RLRNQDPKSKYTYELVAQDDECKPNVGVQVATKVAADKNIVAGVTHYCSAVAMATAGVYN
EECCCCCCCCEEEEEEECCCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RFGMPAVVWGAVLPEVTYGNNFQEVHRVNGTMINQNEVAYKFMAGLGYKKWAIIHDTTDY
HCCCHHHHHHHHCCCCCCCCCHHHHHHCCCEEECCHHHHHHHHHCCCCCEEEEEECCCCC
GKGHNKYFSEFLKKGGGTVLGTFGVTADQQDFTTELTKIRELNPDVIYFGGLTPLGVRIR
CCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHH
TQMEKLGIKAQFQGTSGIKSDAYIQGAGKELAEGTLSFIEGAPWEKLPGGLFFISKYAQQ
HHHHHCCCEEEECCCCCCCCCCEEECCCHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHH
KYGESAEAYGPFAFAAANLIMDAVEKVGPDRKKVRDVLNRTKDADTIVGKVTFDDHRQNI
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCHH
VPLITKYVVENGAWVVWEDSSYGKGKHKLAGL
HHHHHHHHHHCCCEEEECCCCCCCCCHHCCCC
>Mature Secondary Structure
MRRRNTILAGTALLTTCLSLALLASPVMAKEVVKIAFVGPLTGGVSNMGLGGRNSADLAV
CCCCCEEEEHHHHHHHHHHHHHHHCHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCEEEE
RLRNQDPKSKYTYELVAQDDECKPNVGVQVATKVAADKNIVAGVTHYCSAVAMATAGVYN
EECCCCCCCCEEEEEEECCCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RFGMPAVVWGAVLPEVTYGNNFQEVHRVNGTMINQNEVAYKFMAGLGYKKWAIIHDTTDY
HCCCHHHHHHHHCCCCCCCCCHHHHHHCCCEEECCHHHHHHHHHCCCCCEEEEEECCCCC
GKGHNKYFSEFLKKGGGTVLGTFGVTADQQDFTTELTKIRELNPDVIYFGGLTPLGVRIR
CCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHH
TQMEKLGIKAQFQGTSGIKSDAYIQGAGKELAEGTLSFIEGAPWEKLPGGLFFISKYAQQ
HHHHHCCCEEEECCCCCCCCCCEEECCCHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHH
KYGESAEAYGPFAFAAANLIMDAVEKVGPDRKKVRDVLNRTKDADTIVGKVTFDDHRQNI
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCHH
VPLITKYVVENGAWVVWEDSSYGKGKHKLAGL
HHHHHHHHHHCCCEEEECCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA