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The map label for this gene is braE [H]

Identifier: 91789190

GI number: 91789190

Start: 3527994

End: 3529160

Strand: Reverse

Name: braE [H]

Synonym: Bpro_3334

Alternate gene names: 91789190

Gene position: 3529160-3527994 (Counterclockwise)

Preceding gene: 91789191

Following gene: 91789189

Centisome position: 67.87

GC content: 57.24

Gene sequence:

>1167_bases
ATGAAACAACAAGAATCCATGACTCCCGCGAGATCTGCCTTGATGCTCAGCGTCGCGGCGCTTGTCCTGACCGTATATGT
GTGGTTTTTCCTGCATGCAGAGAGTCAAATCGGCATTGCCATTTTGCTGGTTGCCGCAATCGTGGCTGTAATTTTGAGTC
GCCGATTCGGTGTGACGCTGCTGGTCGAGCAGTCGGCCGCGCGGCGCCCGGGCCTGTCCCGATGGTTGGCCGTTGGCTGC
GGTGTGGCCTTGATAGCGGCTTTCCACAGCTCACACTTCGTGTTGTTGATGCTGTGCGGCGTGTTGCTGTACTCCACGGC
CTGTCTCGGTTTGACGGTGCAATTCGGATACTCGGGCGTGGCCAACTTTGCCGGGGCCGCATTTTTTGGCATTGGCAGTT
ACGCCACGGCCGTGCTCGCATCCCACACGGCCATTCCCCACGCCCTCGTCATCCTGATCTCTGGCCTGAGTGCCGCCGTC
ATCGGTTCGCTGCTGATTCTGCCGGTGTTGCGAACGCGCGGCCACTATGCGGCGCTGGTCACCATCGCGTTCGGTATCCT
GTTCAAGACCTTCATCGAGGTCAACGATGTGCTGGGTGGGCCGCAAGGCTTGCAGGTGCCTGGAATGAAGTTGTTCGGCT
TGTCGTTGAACGAGGGCTTCAGCCTGTTCGGCCTGGAGATCTCTTTCTATGTCACCTACGCATTGCTCAGTCTTGCCATG
TTCGCGGGGGTCTATGCCCTGGTCAACGCACTGGAGCGGTCCTGGGTCGGCCTGAGCATGGACGTGGTGCGAACCGATGA
AACAGCGGCATCCACCTTCGGCCTGCACATCGTTCGCTGGAAGGTCATCGCCTTCACCATGGGCAACTTTTTCGCGGGTA
TCGCCGGCGCTCTTTACGGGATGATCATTGCATTTGTCGCGCCCAACAACTTCACTTTTGGCGACTCGCTGCTGATGCTC
TCGATTGTCATTTTGGGTGGCCTGGGCAATATTGCCGGCTTGATTCCGGCCGCGATTATTGTGCTGATTCTTCCTGAAAA
ACTTCAAGTCATTCAGGAATACCGTTTCCTCTTGTATGCGGCGCTGGTGATTGTGATTTTGCTGTTTAGGTCGCAGGGGC
TGCTGCCGCGCAAGACACGGATTTTCTTCGCCAGGAAGGGTTCATGA

Upstream 100 bases:

>100_bases
TCTTACAAGGATGTTTTTGCCTTCGCCGCCGTCATCATTTTTATGGCCTGGAAACCGACTGGTCTGATCGCCGAAAAGTC
GAGCGAGCGAGTTTAGTACC

Downstream 100 bases:

>100_bases
TGGCCGGCCAGTCCCTGATCTCGGTTGACAGGCTGACCATGCGTTTTGGTGGCCTGACGGCGCTCGATGAACTGTCCATG
ACTGTGCATGAGGGAGAAAT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MKQQESMTPARSALMLSVAALVLTVYVWFFLHAESQIGIAILLVAAIVAVILSRRFGVTLLVEQSAARRPGLSRWLAVGC
GVALIAAFHSSHFVLLMLCGVLLYSTACLGLTVQFGYSGVANFAGAAFFGIGSYATAVLASHTAIPHALVILISGLSAAV
IGSLLILPVLRTRGHYAALVTIAFGILFKTFIEVNDVLGGPQGLQVPGMKLFGLSLNEGFSLFGLEISFYVTYALLSLAM
FAGVYALVNALERSWVGLSMDVVRTDETAASTFGLHIVRWKVIAFTMGNFFAGIAGALYGMIIAFVAPNNFTFGDSLLML
SIVILGGLGNIAGLIPAAIIVLILPEKLQVIQEYRFLLYAALVIVILLFRSQGLLPRKTRIFFARKGS

Sequences:

>Translated_388_residues
MKQQESMTPARSALMLSVAALVLTVYVWFFLHAESQIGIAILLVAAIVAVILSRRFGVTLLVEQSAARRPGLSRWLAVGC
GVALIAAFHSSHFVLLMLCGVLLYSTACLGLTVQFGYSGVANFAGAAFFGIGSYATAVLASHTAIPHALVILISGLSAAV
IGSLLILPVLRTRGHYAALVTIAFGILFKTFIEVNDVLGGPQGLQVPGMKLFGLSLNEGFSLFGLEISFYVTYALLSLAM
FAGVYALVNALERSWVGLSMDVVRTDETAASTFGLHIVRWKVIAFTMGNFFAGIAGALYGMIIAFVAPNNFTFGDSLLML
SIVILGGLGNIAGLIPAAIIVLILPEKLQVIQEYRFLLYAALVIVILLFRSQGLLPRKTRIFFARKGS
>Mature_388_residues
MKQQESMTPARSALMLSVAALVLTVYVWFFLHAESQIGIAILLVAAIVAVILSRRFGVTLLVEQSAARRPGLSRWLAVGC
GVALIAAFHSSHFVLLMLCGVLLYSTACLGLTVQFGYSGVANFAGAAFFGIGSYATAVLASHTAIPHALVILISGLSAAV
IGSLLILPVLRTRGHYAALVTIAFGILFKTFIEVNDVLGGPQGLQVPGMKLFGLSLNEGFSLFGLEISFYVTYALLSLAM
FAGVYALVNALERSWVGLSMDVVRTDETAASTFGLHIVRWKVIAFTMGNFFAGIAGALYGMIIAFVAPNNFTFGDSLLML
SIVILGGLGNIAGLIPAAIIVLILPEKLQVIQEYRFLLYAALVIVILLFRSQGLLPRKTRIFFARKGS

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=290, Percent_Identity=35.5172413793103, Blast_Score=142, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 41429; Mature: 41429

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQQESMTPARSALMLSVAALVLTVYVWFFLHAESQIGIAILLVAAIVAVILSRRFGVTL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEE
LVEQSAARRPGLSRWLAVGCGVALIAAFHSSHFVLLMLCGVLLYSTACLGLTVQFGYSGV
EEECHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEECCCCHH
ANFAGAAFFGIGSYATAVLASHTAIPHALVILISGLSAAVIGSLLILPVLRTRGHYAALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
TIAFGILFKTFIEVNDVLGGPQGLQVPGMKLFGLSLNEGFSLFGLEISFYVTYALLSLAM
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHH
FAGVYALVNALERSWVGLSMDVVRTDETAASTFGLHIVRWKVIAFTMGNFFAGIAGALYG
HHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MIIAFVAPNNFTFGDSLLMLSIVILGGLGNIAGLIPAAIIVLILPEKLQVIQEYRFLLYA
HHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ALVIVILLFRSQGLLPRKTRIFFARKGS
HHHHHHHHHHCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MKQQESMTPARSALMLSVAALVLTVYVWFFLHAESQIGIAILLVAAIVAVILSRRFGVTL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEE
LVEQSAARRPGLSRWLAVGCGVALIAAFHSSHFVLLMLCGVLLYSTACLGLTVQFGYSGV
EEECHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEECCCCHH
ANFAGAAFFGIGSYATAVLASHTAIPHALVILISGLSAAVIGSLLILPVLRTRGHYAALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
TIAFGILFKTFIEVNDVLGGPQGLQVPGMKLFGLSLNEGFSLFGLEISFYVTYALLSLAM
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHH
FAGVYALVNALERSWVGLSMDVVRTDETAASTFGLHIVRWKVIAFTMGNFFAGIAGALYG
HHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MIIAFVAPNNFTFGDSLLMLSIVILGGLGNIAGLIPAAIIVLILPEKLQVIQEYRFLLYA
HHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ALVIVILLFRSQGLLPRKTRIFFARKGS
HHHHHHHHHHCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]