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The map label for this gene is nrtB [H]

Identifier: 91789128

GI number: 91789128

Start: 3459144

End: 3460130

Strand: Direct

Name: nrtB [H]

Synonym: Bpro_3268

Alternate gene names: 91789128

Gene position: 3459144-3460130 (Clockwise)

Preceding gene: 91789127

Following gene: 91789129

Centisome position: 66.52

GC content: 63.53

Gene sequence:

>987_bases
ATGGTCAGTGCCGTATTCCACAATCCGCTGGACGCCGCAGCAGCGCCAGCCGCTCGAAGCAGCGAACGCAGTGCTGCCAA
TTCAATAGCAGCCACCGCGCACAAACCCGGGGCGCCAGCCCTCAATGACATGAAGAATCCAGCCCCATCCGCCACCGCGG
TCCCAGTGGCCGCAGCCGCACCTGCCGCGTCCCGGCAGCCATGGGACTCTTCCTTTCTCTGGCTCGCCATCCTGCCACCC
CTGCTGGGCCTGGGCCTGCTGGTGTTGGTGTGGGAGCTGGTCTCCGTGGGCACTGGCGCCAGCATTCCCTCGCCCAGGGA
CACCTTCACGCAGGCCGTGCAGGTGTTCAGCGACCCCTTCTACAACAAGGGACCGAATGACCAGGGCGTGGGCTGGAACG
TGCTGTCGTCGCTCAAACGCGTGGCCATGGGCTTCGGCATGGCGGCCATCGTCGGCATTCCGGTCGGCTTCATGATTGGC
CGCTTCGACTTTTTGAGCCGCATGTTCAACCCGCTGATCAGCCTGCTGCGCCCCGTCTCGCCGCTGGCCTGGCTGCCGAT
CGGCCTGCTGGTGTTCAAGGGCGCCAACCCGGCCGCCATCTGGACCATCTTCATCTGCTCGATCTGGCCCATGATCATCA
ACACCGCCGTGGGCGTGCAGCGCGTGCCCCAGGACTACATGAACGTGGCGCGGGTGCTCAACCTGTCGGAGTGGAAGATC
GTCAGCAAGATCCTCTTTCCTGCCGTGCTGCCCTACATGCTGACCGGTGTGCGCCTGGCCGTCGGCACCGCCTGGCTGGT
GATCGTGGCAGCCGAAATGCTGACCGGTGGCGTCGGCATCGGCTTCTGGGTCTGGGACGAGTGGAACAACCTGAACGTCA
AGAACATCATCATCGCGATTTTCGTGATCGGCATTGTGGGGCTGGTGCTGGAGTTTGCGCTGATCAAGCTCGCCACGGCC
TTCACGTTCGAAGAAGTGAAGAACTGA

Upstream 100 bases:

>100_bases
GGGACGGCAAGGATCCGAAAAAGTACGCCGATGGCTTCAAGGTCCGCGCCTGAAATAGCCGCCGAGCATTCATCAATATC
CCACCAAAGGACGTGACACC

Downstream 100 bases:

>100_bases
TAACCCCCAAACAACCGAGGATGACGACCATGAGCACATCTTTCACCACCAAGTACATCGAAATCCAGGGCGTCGAGCAG
ACCTTCAAGACCAAGAAAGG

Product: nitrate transport permease

Products: taurine [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MVSAVFHNPLDAAAAPAARSSERSAANSIAATAHKPGAPALNDMKNPAPSATAVPVAAAAPAASRQPWDSSFLWLAILPP
LLGLGLLVLVWELVSVGTGASIPSPRDTFTQAVQVFSDPFYNKGPNDQGVGWNVLSSLKRVAMGFGMAAIVGIPVGFMIG
RFDFLSRMFNPLISLLRPVSPLAWLPIGLLVFKGANPAAIWTIFICSIWPMIINTAVGVQRVPQDYMNVARVLNLSEWKI
VSKILFPAVLPYMLTGVRLAVGTAWLVIVAAEMLTGGVGIGFWVWDEWNNLNVKNIIIAIFVIGIVGLVLEFALIKLATA
FTFEEVKN

Sequences:

>Translated_328_residues
MVSAVFHNPLDAAAAPAARSSERSAANSIAATAHKPGAPALNDMKNPAPSATAVPVAAAAPAASRQPWDSSFLWLAILPP
LLGLGLLVLVWELVSVGTGASIPSPRDTFTQAVQVFSDPFYNKGPNDQGVGWNVLSSLKRVAMGFGMAAIVGIPVGFMIG
RFDFLSRMFNPLISLLRPVSPLAWLPIGLLVFKGANPAAIWTIFICSIWPMIINTAVGVQRVPQDYMNVARVLNLSEWKI
VSKILFPAVLPYMLTGVRLAVGTAWLVIVAAEMLTGGVGIGFWVWDEWNNLNVKNIIIAIFVIGIVGLVLEFALIKLATA
FTFEEVKN
>Mature_328_residues
MVSAVFHNPLDAAAAPAARSSERSAANSIAATAHKPGAPALNDMKNPAPSATAVPVAAAAPAASRQPWDSSFLWLAILPP
LLGLGLLVLVWELVSVGTGASIPSPRDTFTQAVQVFSDPFYNKGPNDQGVGWNVLSSLKRVAMGFGMAAIVGIPVGFMIG
RFDFLSRMFNPLISLLRPVSPLAWLPIGLLVFKGANPAAIWTIFICSIWPMIINTAVGVQRVPQDYMNVARVLNLSEWKI
VSKILFPAVLPYMLTGVRLAVGTAWLVIVAAEMLTGGVGIGFWVWDEWNNLNVKNIIIAIFVIGIVGLVLEFALIKLATA
FTFEEVKN

Specific function: Probably part of a high-affinity binding-protein- dependent transport system for nitrate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0600

COG function: function code P; ABC-type nitrate/sulfonate/bicarbonate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786564, Length=212, Percent_Identity=30.188679245283, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87081802, Length=142, Percent_Identity=28.169014084507, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005889 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35084; Mature: 35084

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSAVFHNPLDAAAAPAARSSERSAANSIAATAHKPGAPALNDMKNPAPSATAVPVAAAA
CCCCHHCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCHHHCC
PAASRQPWDSSFLWLAILPPLLGLGLLVLVWELVSVGTGASIPSPRDTFTQAVQVFSDPF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
YNKGPNDQGVGWNVLSSLKRVAMGFGMAAIVGIPVGFMIGRFDFLSRMFNPLISLLRPVS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLAWLPIGLLVFKGANPAAIWTIFICSIWPMIINTAVGVQRVPQDYMNVARVLNLSEWKI
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHH
VSKILFPAVLPYMLTGVRLAVGTAWLVIVAAEMLTGGVGIGFWVWDEWNNLNVKNIIIAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHH
FVIGIVGLVLEFALIKLATAFTFEEVKN
HHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVSAVFHNPLDAAAAPAARSSERSAANSIAATAHKPGAPALNDMKNPAPSATAVPVAAAA
CCCCHHCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCHHHCC
PAASRQPWDSSFLWLAILPPLLGLGLLVLVWELVSVGTGASIPSPRDTFTQAVQVFSDPF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
YNKGPNDQGVGWNVLSSLKRVAMGFGMAAIVGIPVGFMIGRFDFLSRMFNPLISLLRPVS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLAWLPIGLLVFKGANPAAIWTIFICSIWPMIINTAVGVQRVPQDYMNVARVLNLSEWKI
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHH
VSKILFPAVLPYMLTGVRLAVGTAWLVIVAAEMLTGGVGIGFWVWDEWNNLNVKNIIIAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHH
FVIGIVGLVLEFALIKLATAFTFEEVKN
HHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: taurine [Periplasm]; ATP; H2O [C]

Specific reaction: taurine [Periplasm] + ATP + H2O = taurine [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]