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The map label for this gene is nuoB

Identifier: 91789115

GI number: 91789115

Start: 3446215

End: 3446694

Strand: Reverse

Name: nuoB

Synonym: Bpro_3255

Alternate gene names: 91789115

Gene position: 3446694-3446215 (Counterclockwise)

Preceding gene: 91789116

Following gene: 91789114

Centisome position: 66.28

GC content: 61.25

Gene sequence:

>480_bases
ATGTCACTTGAAGGTGTTTTCAACGAAGGCTTCATGACTACGAGTTACGACTCGGTGGTCAACTGGGCCAAGACCGGCTC
GCTGTGGCCCATGACTTTTGGTCTGGCCTGCTGCGCCGTGGAAATGATGCATGCGGGCGCGGCCCGTTATGACATGGACC
GTTTCGGCATGCTGTTTCGGCCCAGCCCGCGCCAGTCCGACCTGATGATTGTTGCCGGCACGCTGTGCAACAAGATGGGG
CCCGCCCTGCGCAAGGTGTACGACCAGATGTCCGAGCCGCGCTGGGTGCTCTCCATGGGCTCCTGCGCCAACGGAGGCGG
TTACTACCACTACAGTTATTCCGTGGTTCGCGGTTGCGACCGCATCGTTCCGGTGGATGTCTATGTGCCGGGCTGCCCGC
CCACCGCCGAAGCGCTGTTGTACGGCATCATCCAGTTGCAGCAGAAAATCCGCCGCACCAACACGATCGCGAGGGCCTGA

Upstream 100 bases:

>100_bases
CTACGAGTGGAAAAAGGGTGCGCTGGACTGGGAATAAGAAAAGATCCCGAGGGTTCTTCGCGACGAGGTCGCCACCAAAT
CCACCAAATAAGGTACGGGT

Downstream 100 bases:

>100_bases
TGCTGTCCTCGTTTCCCGTTTCCGCCACGCAGCTTGGCGACACCATCGCGGCGGTGCTCGGCGACAAGGTCAAGAGCCTG
AAGATCGCGCTGGGTGAGGT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MSLEGVFNEGFMTTSYDSVVNWAKTGSLWPMTFGLACCAVEMMHAGAARYDMDRFGMLFRPSPRQSDLMIVAGTLCNKMG
PALRKVYDQMSEPRWVLSMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIIQLQQKIRRTNTIARA

Sequences:

>Translated_159_residues
MSLEGVFNEGFMTTSYDSVVNWAKTGSLWPMTFGLACCAVEMMHAGAARYDMDRFGMLFRPSPRQSDLMIVAGTLCNKMG
PALRKVYDQMSEPRWVLSMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIIQLQQKIRRTNTIARA
>Mature_158_residues
SLEGVFNEGFMTTSYDSVVNWAKTGSLWPMTFGLACCAVEMMHAGAARYDMDRFGMLFRPSPRQSDLMIVAGTLCNKMGP
ALRKVYDQMSEPRWVLSMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIIQLQQKIRRTNTIARA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=146, Percent_Identity=78.0821917808219, Blast_Score=254, Evalue=2e-68,
Organism=Escherichia coli, GI1788624, Length=149, Percent_Identity=50.3355704697987, Blast_Score=164, Evalue=2e-42,
Organism=Escherichia coli, GI1789074, Length=118, Percent_Identity=42.3728813559322, Blast_Score=98, Evalue=3e-22,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=40.1639344262295, Blast_Score=90, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=136, Percent_Identity=74.2647058823529, Blast_Score=238, Evalue=1e-63,
Organism=Drosophila melanogaster, GI18859983, Length=140, Percent_Identity=76.4285714285714, Blast_Score=252, Evalue=7e-68,
Organism=Drosophila melanogaster, GI24642371, Length=140, Percent_Identity=76.4285714285714, Blast_Score=252, Evalue=7e-68,
Organism=Drosophila melanogaster, GI24651058, Length=140, Percent_Identity=77.1428571428572, Blast_Score=247, Evalue=2e-66,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_POLSJ (Q127X3)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_550067.1
- ProteinModelPortal:   Q127X3
- SMR:   Q127X3
- STRING:   Q127X3
- GeneID:   4014063
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_3255
- NMPDR:   fig|296591.1.peg.1195
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   GKPIGSN
- PhylomeDB:   Q127X3
- ProtClustDB:   PRK06411
- BioCyc:   PSP296591:BPRO_3255-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 17644; Mature: 17513

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
6.9 %Met     (Translated Protein)
10.7 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
6.3 %Met     (Mature Protein)
10.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLEGVFNEGFMTTSYDSVVNWAKTGSLWPMTFGLACCAVEMMHAGAARYDMDRFGMLFR
CCCCCCCCCCCEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHCCHHHCCEEEC
PSPRQSDLMIVAGTLCNKMGPALRKVYDQMSEPRWVLSMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHCCC
RIVPVDVYVPGCPPTAEALLYGIIQLQQKIRRTNTIARA
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLEGVFNEGFMTTSYDSVVNWAKTGSLWPMTFGLACCAVEMMHAGAARYDMDRFGMLFR
CCCCCCCCCCEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHCCHHHCCEEEC
PSPRQSDLMIVAGTLCNKMGPALRKVYDQMSEPRWVLSMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHCCC
RIVPVDVYVPGCPPTAEALLYGIIQLQQKIRRTNTIARA
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA