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The map label for this gene is phnA [H]

Identifier: 91789080

GI number: 91789080

Start: 3408234

End: 3409460

Strand: Direct

Name: phnA [H]

Synonym: Bpro_3220

Alternate gene names: 91789080

Gene position: 3408234-3409460 (Clockwise)

Preceding gene: 91789079

Following gene: 91789081

Centisome position: 65.54

GC content: 64.38

Gene sequence:

>1227_bases
ATGAACAAAGACATTACCGTGAACGACAGGCGCTACCAATGGATGGACCACCCGGTGGTGGTGGTCTGCGTGGACGGCTG
CCAGTACGAATACATCACCGCCGCCGTGGCGGCCGGCGTGGCACCCTTCCTGGGCAGGCTGCTGGCCGGGCGCGGCACCT
GCTACATGGCGGACTGCGTCATTCCAAGTTTCACCAACCCGAACAACCTGTCCATCGTGACGGGTGCGCCACCCTCGGTG
CACGGCATCTGCGGCAACTACTTCTTCGACCGCGAGCGGGGTGAAGAGGTGATGATGAACGACCCCCGCTACCTGCGCGC
GGGCACCATCCTGGCAGCCTTCGCCGATGCGGGCGCCAAGGTGGCCGTGATCACGGCGAAGGACAAACTGCGCAAGCTGC
TGGGCCACAAAATGGACGGCATCTGCTTTTCCTCGGAAAAGGCCGACCAGGTGAGCGTCGAGGAAAACGGCATTGACGAC
GTGCTCGGCATGGTCGGCATGCCGGTGCCTTCGGTCTACAGCGCCGAGCTGTCGGAGTTCGTGTTTGTCGCCGGCGTCAA
GCTGATGGAGCGCGAGCGGCCTGACCTGATGTATTTGTCCACTACCGACTACATCCAGCACAAGGCAGCCCCCGGCACCA
CGATGGCAAACGACTTCTACGCCATGATGGATCGCAACCTCGCCCGTCTGGACATGCTGGGCGCCACCATTGTGCTCACC
GCGGACCACGGCATGAACGACAAGCACGGCCGGGACGGGCAGCCCAATGTGATTTACCTGCAGGACACGCTGGACGAATG
GATTGGCCAGGACCATGCCCGCGTCATCCTGCCCATCACCGACCCGTATGTGGTGCACCACGGCGCGCTCGGCTCATTCG
CGCTCGTCTATGTGCCGGAAGATGTGGACGCCAGCGCGCTGCGTGACCGCATTGCCGCGCTGCCAGGCATTGAGACGGTG
CTGTCGCGCCTGGAGGCCTGCGACCATTTCGAGCTGCCGTTCGACCGGGTTGGAGACATCGTGGTGGTGGCAAGCCAGGA
CACAGTGATCGGCACCAACGCCGAGCGCCATGACCTCTCGGGGCTGGACGTGCCGCTGCGCTCCCACGGGGGCGTGTCGG
AGCAGAAAGTGCCGCTGGTCGTCAACCGCCAGGTGCCCCAGGCCTTCCCCGACCGGCGCTGGCGCAATTTTGACGCCTTC
GAGCTCGGCCTCAACCTGGCCCGCTGA

Upstream 100 bases:

>100_bases
ATGCACAAGGCTTTTGGCAGAGGCGCCAGTGAGCCAGTCTCTCCCGCAATTTCCCTGAAAAAACCACGGCCCATCATGGC
CATCAACCCCTGAGGACATC

Downstream 100 bases:

>100_bases
TCCACCGCAAAAACATCACCGAACCGCCACAGAAACAGAAACAAGGAACCAACATGTCTGCCGTACTTGCCGATGCTCCT
TCCCAGGCACTTCCTTCCCG

Product: phosphonoacetate hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MNKDITVNDRRYQWMDHPVVVVCVDGCQYEYITAAVAAGVAPFLGRLLAGRGTCYMADCVIPSFTNPNNLSIVTGAPPSV
HGICGNYFFDRERGEEVMMNDPRYLRAGTILAAFADAGAKVAVITAKDKLRKLLGHKMDGICFSSEKADQVSVEENGIDD
VLGMVGMPVPSVYSAELSEFVFVAGVKLMERERPDLMYLSTTDYIQHKAAPGTTMANDFYAMMDRNLARLDMLGATIVLT
ADHGMNDKHGRDGQPNVIYLQDTLDEWIGQDHARVILPITDPYVVHHGALGSFALVYVPEDVDASALRDRIAALPGIETV
LSRLEACDHFELPFDRVGDIVVVASQDTVIGTNAERHDLSGLDVPLRSHGGVSEQKVPLVVNRQVPQAFPDRRWRNFDAF
ELGLNLAR

Sequences:

>Translated_408_residues
MNKDITVNDRRYQWMDHPVVVVCVDGCQYEYITAAVAAGVAPFLGRLLAGRGTCYMADCVIPSFTNPNNLSIVTGAPPSV
HGICGNYFFDRERGEEVMMNDPRYLRAGTILAAFADAGAKVAVITAKDKLRKLLGHKMDGICFSSEKADQVSVEENGIDD
VLGMVGMPVPSVYSAELSEFVFVAGVKLMERERPDLMYLSTTDYIQHKAAPGTTMANDFYAMMDRNLARLDMLGATIVLT
ADHGMNDKHGRDGQPNVIYLQDTLDEWIGQDHARVILPITDPYVVHHGALGSFALVYVPEDVDASALRDRIAALPGIETV
LSRLEACDHFELPFDRVGDIVVVASQDTVIGTNAERHDLSGLDVPLRSHGGVSEQKVPLVVNRQVPQAFPDRRWRNFDAF
ELGLNLAR
>Mature_408_residues
MNKDITVNDRRYQWMDHPVVVVCVDGCQYEYITAAVAAGVAPFLGRLLAGRGTCYMADCVIPSFTNPNNLSIVTGAPPSV
HGICGNYFFDRERGEEVMMNDPRYLRAGTILAAFADAGAKVAVITAKDKLRKLLGHKMDGICFSSEKADQVSVEENGIDD
VLGMVGMPVPSVYSAELSEFVFVAGVKLMERERPDLMYLSTTDYIQHKAAPGTTMANDFYAMMDRNLARLDMLGATIVLT
ADHGMNDKHGRDGQPNVIYLQDTLDEWIGQDHARVILPITDPYVVHHGALGSFALVYVPEDVDASALRDRIAALPGIETV
LSRLEACDHFELPFDRVGDIVVVASQDTVIGTNAERHDLSGLDVPLRSHGGVSEQKVPLVVNRQVPQAFPDRRWRNFDAF
ELGLNLAR

Specific function: Specifically hydrolyzes phosphonoacetate. Does not have activity on other organophosphonates or acetates [H]

COG id: COG1524

COG function: function code R; Uncharacterized proteins of the AP superfamily

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family. PhnA subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR002591
- InterPro:   IPR012710
- InterPro:   IPR023116 [H]

Pfam domain/function: PF01663 Phosphodiest [H]

EC number: =3.11.1.2 [H]

Molecular weight: Translated: 44855; Mature: 44855

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKDITVNDRRYQWMDHPVVVVCVDGCQYEYITAAVAAGVAPFLGRLLAGRGTCYMADCV
CCCCEEECCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
IPSFTNPNNLSIVTGAPPSVHGICGNYFFDRERGEEVMMNDPRYLRAGTILAAFADAGAK
CCCCCCCCCEEEEECCCCCCCCCCCCCEECCCCCCEEEECCCCEEEHHHEEEEECCCCCE
VAVITAKDKLRKLLGHKMDGICFSSEKADQVSVEENGIDDVLGMVGMPVPSVYSAELSEF
EEEEECHHHHHHHHCCCCCCEEECCCCCCEEECCCCCHHHHHHHHCCCCCCHHHHHHHHH
VFVAGVKLMERERPDLMYLSTTDYIQHKAAPGTTMANDFYAMMDRNLARLDMLGATIVLT
HHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHCCEEEEEE
ADHGMNDKHGRDGQPNVIYLQDTLDEWIGQDHARVILPITDPYVVHHGALGSFALVYVPE
ECCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCCEEEEEECCCEEEECCCCCCEEEEEECC
DVDASALRDRIAALPGIETVLSRLEACDHFELPFDRVGDIVVVASQDTVIGTNAERHDLS
CCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHCCCEEEEECCCEEEECCCCCCCCC
GLDVPLRSHGGVSEQKVPLVVNRQVPQAFPDRRWRNFDAFELGLNLAR
CCCCCHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHCCCCCC
>Mature Secondary Structure
MNKDITVNDRRYQWMDHPVVVVCVDGCQYEYITAAVAAGVAPFLGRLLAGRGTCYMADCV
CCCCEEECCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
IPSFTNPNNLSIVTGAPPSVHGICGNYFFDRERGEEVMMNDPRYLRAGTILAAFADAGAK
CCCCCCCCCEEEEECCCCCCCCCCCCCEECCCCCCEEEECCCCEEEHHHEEEEECCCCCE
VAVITAKDKLRKLLGHKMDGICFSSEKADQVSVEENGIDDVLGMVGMPVPSVYSAELSEF
EEEEECHHHHHHHHCCCCCCEEECCCCCCEEECCCCCHHHHHHHHCCCCCCHHHHHHHHH
VFVAGVKLMERERPDLMYLSTTDYIQHKAAPGTTMANDFYAMMDRNLARLDMLGATIVLT
HHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHCCEEEEEE
ADHGMNDKHGRDGQPNVIYLQDTLDEWIGQDHARVILPITDPYVVHHGALGSFALVYVPE
ECCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCCEEEEEECCCEEEECCCCCCEEEEEECC
DVDASALRDRIAALPGIETVLSRLEACDHFELPFDRVGDIVVVASQDTVIGTNAERHDLS
CCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHCCCEEEEECCCEEEECCCCCCCCC
GLDVPLRSHGGVSEQKVPLVVNRQVPQAFPDRRWRNFDAFELGLNLAR
CCCCCHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA