| Definition | Polaromonas sp. JS666 chromosome, complete genome. |
|---|---|
| Accession | NC_007948 |
| Length | 5,200,264 |
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The map label for this gene is amiC [H]
Identifier: 91789059
GI number: 91789059
Start: 3383238
End: 3384653
Strand: Direct
Name: amiC [H]
Synonym: Bpro_3199
Alternate gene names: 91789059
Gene position: 3383238-3384653 (Clockwise)
Preceding gene: 91789058
Following gene: 91789060
Centisome position: 65.06
GC content: 66.53
Gene sequence:
>1416_bases ATGACGAATAAAAGCGGTCGCCACTTCAACCGGCGCAGCGCCTTGCAGCTGGGCGGCGTGGTCCTGCTGCTGGGCGCGCA GCACATTGCCCGTGGCGCCACCATTGTCGCCGTGCGCGTCTGGCCGGCCAAAGACTACACCCGGGTCACCATCGAGTCCG ACGGTGAAATCAAGGCGCGGCAGTTCTTTGTGGCCGAGCCGCCGCGCCTGGCGGTTGACATTGAGGGCATAGACCTGATT CCGGCCTTGCGCGAGTTGGTGGCCAAGGTGAAATCGGACGATCCGAATATTTCAGGGATTCGCGTCGGGCAAAACGCACC AGGCGTGGTGCGGCTGGTGATTGACCTGAAGCAGGCCATGCTGCCGCAGGTCTTCACGCTTCCGCCGGTGGCTGCTTACC AGCACCGGCTGGTGCTGGACCTGTACCCGGCGCAGGCCATCGACCCGCTGGAGGCCCTGATTGCCGAGCGGCTGGCCGAC AAGCAGGCCGGCGCCGGCTCATCCACGAGCGCAGCCCCCGACCCGCTGGGCGAGTTGATCGCCCGCCAGACCGGACGAGC CCCATCGGGCGCAATGGCCGGTGGCGACAAGCCAGCGGGCAACCCGCCGCTGGCTGGCTCTGCAGACGACCGCTTGCCCT TACCGGCCGCCCGGCCGCCTGGCGACATGGCGGCCGCTGGCAAGGGACGGACCGAAGGCAAGACAGACCGGCTCGTCATC GTGGCGCTGGACCCGGGACATGGCGGCGAGGACCCGGGCGCGGTTGGGCCCGGTGGCACACGCGAGAAAGACGTGGTGCT GCAAATCGCGCATCGCCTGCGCGACCGCATCAACCAGCAACCGAACATGCGCGCCTACCTCACGCGCGATGCGGATTTTT TTGTGCCGCTGCACATCCGCGTGCAAAAAGCGCGCCGCGTGCAGGCCGATCTTTTCATCAGCATCCATGCGGACGCCTTC TTTACCGCCCGCCCGCAAGGCGCCAGCGTGTTTGCCTTGAGTGACAAGGGCGCCTCCAGCAGCGCCGCGCGCTGGCTGGC CGAGAAGGAAAATTCCGCCGACCTCGTGGGCGGCGTCAATGTGAAGGCCCGGGACGTGCAGGTCCAGAAGGCCCTGCTGG ACATGAGCACCACCGCGCAGATCAATGACAGCCTCAAGCTGGGCAGCGCCATGCTGGGCGAGATCGGCCATGTCGGCAGG CTGCACAAGCCGCGGGTTGAACAAGCCGGCTTTGCGGTGCTCAAGGCGCCCGACATCCCCAGCGTGCTGGTAGAAACCGC CTTCATCAGCAATCCGGAGGAAGAAGCCCGATTGCGCAGCGATGACTATCAGATCCAGCTCGCGGATGCGCTGATGCGCG GCATCAGCCGCTACTTTGCCCGCAACCCGCCGCTGGCGCGGAATCGCGCTCTGTAG
Upstream 100 bases:
>100_bases TGCCGCCGCCCGACCTGCTGCTCAAGATGGAGGTGTTGGGTGACACGTCCCGTTCAGTCACAGCGACGGCTCACAGTGCC ACCGGCGCAAAACTGCTGGC
Downstream 100 bases:
>100_bases TCACCCTGATCAGTCCTCGCGGACAGCGGGTCCGTGCGCGGCTGCTCCCTGCGGCTTTTTTCAAGCCCAACCGACAGGCG GCAACGGCCCTCTCCTACAG
Product: N-acetylmuramoyl-L-alanine amidase
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 471; Mature: 470
Protein sequence:
>471_residues MTNKSGRHFNRRSALQLGGVVLLLGAQHIARGATIVAVRVWPAKDYTRVTIESDGEIKARQFFVAEPPRLAVDIEGIDLI PALRELVAKVKSDDPNISGIRVGQNAPGVVRLVIDLKQAMLPQVFTLPPVAAYQHRLVLDLYPAQAIDPLEALIAERLAD KQAGAGSSTSAAPDPLGELIARQTGRAPSGAMAGGDKPAGNPPLAGSADDRLPLPAARPPGDMAAAGKGRTEGKTDRLVI VALDPGHGGEDPGAVGPGGTREKDVVLQIAHRLRDRINQQPNMRAYLTRDADFFVPLHIRVQKARRVQADLFISIHADAF FTARPQGASVFALSDKGASSSAARWLAEKENSADLVGGVNVKARDVQVQKALLDMSTTAQINDSLKLGSAMLGEIGHVGR LHKPRVEQAGFAVLKAPDIPSVLVETAFISNPEEEARLRSDDYQIQLADALMRGISRYFARNPPLARNRAL
Sequences:
>Translated_471_residues MTNKSGRHFNRRSALQLGGVVLLLGAQHIARGATIVAVRVWPAKDYTRVTIESDGEIKARQFFVAEPPRLAVDIEGIDLI PALRELVAKVKSDDPNISGIRVGQNAPGVVRLVIDLKQAMLPQVFTLPPVAAYQHRLVLDLYPAQAIDPLEALIAERLAD KQAGAGSSTSAAPDPLGELIARQTGRAPSGAMAGGDKPAGNPPLAGSADDRLPLPAARPPGDMAAAGKGRTEGKTDRLVI VALDPGHGGEDPGAVGPGGTREKDVVLQIAHRLRDRINQQPNMRAYLTRDADFFVPLHIRVQKARRVQADLFISIHADAF FTARPQGASVFALSDKGASSSAARWLAEKENSADLVGGVNVKARDVQVQKALLDMSTTAQINDSLKLGSAMLGEIGHVGR LHKPRVEQAGFAVLKAPDIPSVLVETAFISNPEEEARLRSDDYQIQLADALMRGISRYFARNPPLARNRAL >Mature_470_residues TNKSGRHFNRRSALQLGGVVLLLGAQHIARGATIVAVRVWPAKDYTRVTIESDGEIKARQFFVAEPPRLAVDIEGIDLIP ALRELVAKVKSDDPNISGIRVGQNAPGVVRLVIDLKQAMLPQVFTLPPVAAYQHRLVLDLYPAQAIDPLEALIAERLADK QAGAGSSTSAAPDPLGELIARQTGRAPSGAMAGGDKPAGNPPLAGSADDRLPLPAARPPGDMAAAGKGRTEGKTDRLVIV ALDPGHGGEDPGAVGPGGTREKDVVLQIAHRLRDRINQQPNMRAYLTRDADFFVPLHIRVQKARRVQADLFISIHADAFF TARPQGASVFALSDKGASSSAARWLAEKENSADLVGGVNVKARDVQVQKALLDMSTTAQINDSLKLGSAMLGEIGHVGRL HKPRVEQAGFAVLKAPDIPSVLVETAFISNPEEEARLRSDDYQIQLADALMRGISRYFARNPPLARNRAL
Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]
COG id: COG0860
COG function: function code M; N-acetylmuramoyl-L-alanine amidase
Gene ontology:
Cell location: Periplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]
Homologues:
Organism=Escherichia coli, GI87082163, Length=465, Percent_Identity=45.5913978494624, Blast_Score=387, Evalue=1e-109, Organism=Escherichia coli, GI1788776, Length=220, Percent_Identity=49.0909090909091, Blast_Score=206, Evalue=3e-54, Organism=Escherichia coli, GI1790611, Length=242, Percent_Identity=44.2148760330579, Blast_Score=201, Evalue=8e-53,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR021731 - InterPro: IPR002508 - InterPro: IPR006311 [H]
Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]
EC number: =3.5.1.28 [H]
Molecular weight: Translated: 50375; Mature: 50244
Theoretical pI: Translated: 10.07; Mature: 10.07
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.0 %Cys (Translated Protein) 1.7 %Met (Translated Protein) 1.7 %Cys+Met (Translated Protein) 0.0 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 1.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTNKSGRHFNRRSALQLGGVVLLLGAQHIARGATIVAVRVWPAKDYTRVTIESDGEIKAR CCCCCCCCCCHHHHHHHCCEEEEECCHHHHCCCEEEEEEEECCCCCEEEEEECCCCEEEE QFFVAEPPRLAVDIEGIDLIPALRELVAKVKSDDPNISGIRVGQNAPGVVRLVIDLKQAM EEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCEEHHHHHHHHH LPQVFTLPPVAAYQHRLVLDLYPAQAIDPLEALIAERLADKQAGAGSSTSAAPDPLGELI CCHHCCCCCHHHHCCEEEEEECCCHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH ARQTGRAPSGAMAGGDKPAGNPPLAGSADDRLPLPAARPPGDMAAAGKGRTEGKTDRLVI HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE VALDPGHGGEDPGAVGPGGTREKDVVLQIAHRLRDRINQQPNMRAYLTRDADFFVPLHIR EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEE VQKARRVQADLFISIHADAFFTARPQGASVFALSDKGASSSAARWLAEKENSADLVGGVN EHHHHCCEEEEEEEEECCEEEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEECCC VKARDVQVQKALLDMSTTAQINDSLKLGSAMLGEIGHVGRLHKPRVEQAGFAVLKAPDIP CEEHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCEEEEECCCCH SVLVETAFISNPEEEARLRSDDYQIQLADALMRGISRYFARNPPLARNRAL HHHHHHHHCCCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCC >Mature Secondary Structure TNKSGRHFNRRSALQLGGVVLLLGAQHIARGATIVAVRVWPAKDYTRVTIESDGEIKAR CCCCCCCCCHHHHHHHCCEEEEECCHHHHCCCEEEEEEEECCCCCEEEEEECCCCEEEE QFFVAEPPRLAVDIEGIDLIPALRELVAKVKSDDPNISGIRVGQNAPGVVRLVIDLKQAM EEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCEEHHHHHHHHH LPQVFTLPPVAAYQHRLVLDLYPAQAIDPLEALIAERLADKQAGAGSSTSAAPDPLGELI CCHHCCCCCHHHHCCEEEEEECCCHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH ARQTGRAPSGAMAGGDKPAGNPPLAGSADDRLPLPAARPPGDMAAAGKGRTEGKTDRLVI HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE VALDPGHGGEDPGAVGPGGTREKDVVLQIAHRLRDRINQQPNMRAYLTRDADFFVPLHIR EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEE VQKARRVQADLFISIHADAFFTARPQGASVFALSDKGASSSAARWLAEKENSADLVGGVN EHHHHCCEEEEEEEEECCEEEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEECCC VKARDVQVQKALLDMSTTAQINDSLKLGSAMLGEIGHVGRLHKPRVEQAGFAVLKAPDIP CEEHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCEEEEECCCCH SVLVETAFISNPEEEARLRSDDYQIQLADALMRGISRYFARNPPLARNRAL HHHHHHHHCCCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 12471157 [H]