Click here to switch to the map view.

The map label for this gene is lexA

Identifier: 91788814

GI number: 91788814

Start: 3114757

End: 3115461

Strand: Direct

Name: lexA

Synonym: Bpro_2954

Alternate gene names: 91788814

Gene position: 3114757-3115461 (Clockwise)

Preceding gene: 91788812

Following gene: 91788815

Centisome position: 59.9

GC content: 59.57

Gene sequence:

>705_bases
ATGAGCATATTGAACGATTTTTCGCACTTATCCTCCGAAGGTCCCAAGCTCACGGCACGGCAGCAGCAAATCCTGGAATT
GATCCAGAGCGCCATCACCCGCACCGGCGCGCCGCCAACCCGCGCCGAAATCGCCAACGAATTGGGCTTCAAGTCTGCCA
ACGCCGCTGAAGAGCACCTGCAAGCCCTGGCCCGCAAAGGCGTGATTGAACTGGTCAGCGGCACCTCGCGCGGCATACGC
CTGCGCAGCGATACCTTGCGCTCCATCCACGAATCCAGAGTCAAACAGTTTTCCCTGCCGTTGCAAAGCCTGGCCCAACT
CGCCCTGCCTCTGGTGGGGCGGGTTGCTGCTGGCAGCCCGATTCTCGCGCAGGAACACATCGAGCAGACCTATTATTTTG
AAAGCAGCCTGTTCCAGCGACAGCCCGATTACCTGCTCAAGGTGCGTGGCATGAGCATGCGGGACGCCGGCATCATCGAC
GGCGATCTGCTGGCCGTCAAGCAGGCCAAGGAAGCCAGGAACGGCCAAATTGTCGTGGCCCGTATCGGCGACGAAGTGAC
GGTCAAGCGTTTTAGGCGCACCAAGCATCTGATTGAATTGTTGCCTGAGAACCCGGACTTCAAAACCATTGTGGTCGAGC
CCGGCGAGCCCTTCGAGCTCGAGGGCCTGGCCGTCGGTTTGATCCGCAACACCATGCTCATCTAG

Upstream 100 bases:

>100_bases
CAGTTACTGGATATTAAAACAGGTCACTACAACAGATGACGCACGCGACAACCCCGGGTTGCCCGGCGCACTCAACCTGA
GAAACCAAGGAGTCCTGGTC

Downstream 100 bases:

>100_bases
TCAACCCCTTTCCGGCCGCGCGACAGGTGGCGGGCGTTTGGTCATTGATTGACCAACACCCTGGCGTTGCGGCCCCACTG
GCGATTAGCCAGTCCGAAAT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSILNDFSHLSSEGPKLTARQQQILELIQSAITRTGAPPTRAEIANELGFKSANAAEEHLQALARKGVIELVSGTSRGIR
LRSDTLRSIHESRVKQFSLPLQSLAQLALPLVGRVAAGSPILAQEHIEQTYYFESSLFQRQPDYLLKVRGMSMRDAGIID
GDLLAVKQAKEARNGQIVVARIGDEVTVKRFRRTKHLIELLPENPDFKTIVVEPGEPFELEGLAVGLIRNTMLI

Sequences:

>Translated_234_residues
MSILNDFSHLSSEGPKLTARQQQILELIQSAITRTGAPPTRAEIANELGFKSANAAEEHLQALARKGVIELVSGTSRGIR
LRSDTLRSIHESRVKQFSLPLQSLAQLALPLVGRVAAGSPILAQEHIEQTYYFESSLFQRQPDYLLKVRGMSMRDAGIID
GDLLAVKQAKEARNGQIVVARIGDEVTVKRFRRTKHLIELLPENPDFKTIVVEPGEPFELEGLAVGLIRNTMLI
>Mature_233_residues
SILNDFSHLSSEGPKLTARQQQILELIQSAITRTGAPPTRAEIANELGFKSANAAEEHLQALARKGVIELVSGTSRGIRL
RSDTLRSIHESRVKQFSLPLQSLAQLALPLVGRVAAGSPILAQEHIEQTYYFESSLFQRQPDYLLKVRGMSMRDAGIIDG
DLLAVKQAKEARNGQIVVARIGDEVTVKRFRRTKHLIELLPENPDFKTIVVEPGEPFELEGLAVGLIRNTMLI

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=215, Percent_Identity=57.6744186046512, Blast_Score=236, Evalue=7e-64,
Organism=Escherichia coli, GI1787431, Length=116, Percent_Identity=31.8965517241379, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_POLSJ (Q129C4)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_549766.1
- ProteinModelPortal:   Q129C4
- STRING:   Q129C4
- MEROPS:   S24.001
- GeneID:   4014373
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_2954
- NMPDR:   fig|296591.1.peg.5544
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- PhylomeDB:   Q129C4
- ProtClustDB:   PRK00215
- BioCyc:   PSP296591:BPRO_2954-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 25883; Mature: 25752

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: ACT_SITE 152-152 ACT_SITE 189-189

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILNDFSHLSSEGPKLTARQQQILELIQSAITRTGAPPTRAEIANELGFKSANAAEEHL
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHH
QALARKGVIELVSGTSRGIRLRSDTLRSIHESRVKQFSLPLQSLAQLALPLVGRVAAGSP
HHHHHCCHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
ILAQEHIEQTYYFESSLFQRQPDYLLKVRGMSMRDAGIIDGDLLAVKQAKEARNGQIVVA
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEE
RIGDEVTVKRFRRTKHLIELLPENPDFKTIVVEPGEPFELEGLAVGLIRNTMLI
ECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEECHHHHHHHHHHHCC
>Mature Secondary Structure 
SILNDFSHLSSEGPKLTARQQQILELIQSAITRTGAPPTRAEIANELGFKSANAAEEHL
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHH
QALARKGVIELVSGTSRGIRLRSDTLRSIHESRVKQFSLPLQSLAQLALPLVGRVAAGSP
HHHHHCCHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
ILAQEHIEQTYYFESSLFQRQPDYLLKVRGMSMRDAGIIDGDLLAVKQAKEARNGQIVVA
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEE
RIGDEVTVKRFRRTKHLIELLPENPDFKTIVVEPGEPFELEGLAVGLIRNTMLI
ECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEECHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA