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The map label for this gene is trmB

Identifier: 91788765

GI number: 91788765

Start: 3065214

End: 3065954

Strand: Direct

Name: trmB

Synonym: Bpro_2905

Alternate gene names: 91788765

Gene position: 3065214-3065954 (Clockwise)

Preceding gene: 91788764

Following gene: 91788766

Centisome position: 58.94

GC content: 63.16

Gene sequence:

>741_bases
GTGATTGAAAACCCCTCCCCCAGCGCTGCCAACCTCCCCGAGCACCCGTCCACCGACGCGAGCCACCCCCGCAACATCCG
CAGTTTTGTCCGGCGCACCGGCCGCACGACCGTCGGCCAGGCCAAGGCCTTTGCCGACGTGGGGCCACGGTTTTTACTCG
CCTACAAAGCGGAACCCCTGGACTTCGCAGCCGCCTTTGGGCGTGTGGCCCCCACCATCCTCGAGATCGGCTTCGGCATG
GGCGAGGCCACGGCGCATATTGCGGCCCTGAGCCCCGATAAAAACTTCCTTTGCTGCGAAGTACACACACCCGGCGTCGG
CGCGCTGCTCAAGCGCATTCAGGAGCAGACCCTGGGCAACATCCGCATCCTGCAGCACGATGCGGTCGAAGTCATTGACA
ACATGTTGCCAGCCGGCAGCCTGGACGGCGTTCATATCTTCTTTCCCGACCCCTGGCATAAAAAGAAACACAACAAGCGC
CGGCTGATCCAGGCGCCCCTGATTGCCAAGCTGGCGGCCAGGCTCAAACCCGGCGGCTACCTGCACTGCGCCACCGACTG
GCAGCCTTACGCCGAACAGATTCTCGAAGTCCTGAGCGCTGAAGCCCTGCTGAAAAACACGGCTGATGCATCGGGTGGCG
GTTACGCGCCCAAGCCCGACTACCGCCCCCTGACCAAGTTCGAAAACCGCGGCATCAAGCTCGGGCACGGTGTCTGGGAT
GTGGTGTTCACCCGTGTGTGA

Upstream 100 bases:

>100_bases
GCAGGACAATGCGACGGCCAGTACACCGGCGGCATGGCTGCAGCAGGCTGTGCCCCAGTGGGCAAGCCGTCATGCAGACG
CGGCGCACTAAAATTGCGTT

Downstream 100 bases:

>100_bases
GACATTCCGACAGCCGGTCACGCAATGAATGCCGAAAGCCTGATGATTCACGGGCTTCCCACCCGCAACGGCGTGAGTCC
GAGTTGCGTGGGCCTGCCCG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MIENPSPSAANLPEHPSTDASHPRNIRSFVRRTGRTTVGQAKAFADVGPRFLLAYKAEPLDFAAAFGRVAPTILEIGFGM
GEATAHIAALSPDKNFLCCEVHTPGVGALLKRIQEQTLGNIRILQHDAVEVIDNMLPAGSLDGVHIFFPDPWHKKKHNKR
RLIQAPLIAKLAARLKPGGYLHCATDWQPYAEQILEVLSAEALLKNTADASGGGYAPKPDYRPLTKFENRGIKLGHGVWD
VVFTRV

Sequences:

>Translated_246_residues
MIENPSPSAANLPEHPSTDASHPRNIRSFVRRTGRTTVGQAKAFADVGPRFLLAYKAEPLDFAAAFGRVAPTILEIGFGM
GEATAHIAALSPDKNFLCCEVHTPGVGALLKRIQEQTLGNIRILQHDAVEVIDNMLPAGSLDGVHIFFPDPWHKKKHNKR
RLIQAPLIAKLAARLKPGGYLHCATDWQPYAEQILEVLSAEALLKNTADASGGGYAPKPDYRPLTKFENRGIKLGHGVWD
VVFTRV
>Mature_246_residues
MIENPSPSAANLPEHPSTDASHPRNIRSFVRRTGRTTVGQAKAFADVGPRFLLAYKAEPLDFAAAFGRVAPTILEIGFGM
GEATAHIAALSPDKNFLCCEVHTPGVGALLKRIQEQTLGNIRILQHDAVEVIDNMLPAGSLDGVHIFFPDPWHKKKHNKR
RLIQAPLIAKLAARLKPGGYLHCATDWQPYAEQILEVLSAEALLKNTADASGGGYAPKPDYRPLTKFENRGIKLGHGVWD
VVFTRV

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=223, Percent_Identity=51.1210762331839, Blast_Score=241, Evalue=4e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_POLSJ (Q129H3)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_549717.1
- ProteinModelPortal:   Q129H3
- STRING:   Q129H3
- GeneID:   4014441
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_2905
- NMPDR:   fig|296591.1.peg.5496
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- PhylomeDB:   Q129H3
- ProtClustDB:   PRK00121
- BioCyc:   PSP296591:BPRO_2905-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 26881; Mature: 26881

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: BINDING 75-75 BINDING 100-100 BINDING 127-127 BINDING 150-150 BINDING 154-154 BINDING 186-186

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIENPSPSAANLPEHPSTDASHPRNIRSFVRRTGRTTVGQAKAFADVGPRFLLAYKAEPL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCH
DFAAAFGRVAPTILEIGFGMGEATAHIAALSPDKNFLCCEVHTPGVGALLKRIQEQTLGN
HHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCC
IRILQHDAVEVIDNMLPAGSLDGVHIFFPDPWHKKKHNKRRLIQAPLIAKLAARLKPGGY
EEEEHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LHCATDWQPYAEQILEVLSAEALLKNTADASGGGYAPKPDYRPLTKFENRGIKLGHGVWD
EEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCEEECCCHHH
VVFTRV
HHHCCC
>Mature Secondary Structure
MIENPSPSAANLPEHPSTDASHPRNIRSFVRRTGRTTVGQAKAFADVGPRFLLAYKAEPL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCH
DFAAAFGRVAPTILEIGFGMGEATAHIAALSPDKNFLCCEVHTPGVGALLKRIQEQTLGN
HHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCC
IRILQHDAVEVIDNMLPAGSLDGVHIFFPDPWHKKKHNKRRLIQAPLIAKLAARLKPGGY
EEEEHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LHCATDWQPYAEQILEVLSAEALLKNTADASGGGYAPKPDYRPLTKFENRGIKLGHGVWD
EEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCEEECCCHHH
VVFTRV
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA